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Horizontal Electrophoresis System
4849.1 ROTIPHORESE
4850.1 ROTIPHORESE
electrophoresis unit ROTIPHORESE
9958.1 ROTIPHORESE
electrophoresis unit ROTIPHORESE
9961.1 ROTIPHORESE
electrophoresis unit ROTIPHORESE
runVIEW – made by Cleaver Scientific Ltd, UK
Warning: The runVIEW real-time horizontal DNA electrophoresis system has been thoroughly tested
and found to comply within the limits of CE regulation. It has been manufactured using the latest
technology and does not require maintenance. When used correctly this unit poses no particular
health risk, although it can deliver dangerous voltage levels if used incorrectly. Accordingly, this
power supply must only be operated by fully qualified personnel adhering to the guidelines laid
out within this instruction manual. Although this power supply is equipped with all necessary safety
features against abuse and accidental failure, caution should always be exercised when working with
high voltage equipment. Any individual intending to use this instrument should read the entire manual
thoroughly before operation.
The horizontal electrophoresis units from ROTH are designed for long term laboratory use and to
obtain reproducible results. Please spend a few moments reading the instruction manual thoroughly.
These units comply with the statutory CE safety rules:
73/23/EEC: Low voltage directive: IEC 1010-1:1990 plus amendment 1:1992
EN 61010-1:1993/BS EN 61010-1:1993
Please verify that you received the unit completely and without any damage. Any faults or losses have
to be reported to ROTH immediately. ROTH can not accept responsibility for goods that were sent
back without informing them.
Please take a look at the packing list and check whether all components and accessories are present.
Please retain all packaging material until the warranty period has expired.
Environmental Conditions
This unit may only be installed and operated only under the following environmental conditions:
o
This apparatus is intended for indoor use only.
o
The unit can be operated safely at an altitude of 2000 m.
o
The normal operating temperature range is ambient to 40 °C.
Atmospheric pressure: 75 kPa – 106 kPa
o
Relative humidity: ≤95 %
o
This apparatus is rated POLLUTION DEGREE 2 in accordance with IEC 664.
POLLUTION DEGREE 2, states that: "Normally only non-conductive pollution occurs. Occasionally,
however, a temporary conductivity caused by condensation must be expected".
All ROTH products are supplied having passed rigorous quality control procedures. For
additional questions please give us a call: ++49-721-5606-0
®
PROfessional runVIEW System
®
PROfessional runVIEW Base for
®
PROfessional runVIEW Base MINI 1 for
®
PROfessional runVIEW Base MINI 2 for
1
®
PROfessional III (2850)
®
PROfessional I (2788)
®
PROfessional II (2799)
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Summary of Contents for Roth ROTIPHORESE PROfessional runVIEW
- Page 1 EN 61010-1:1993/BS EN 61010-1:1993 Please verify that you received the unit completely and without any damage. Any faults or losses have to be reported to ROTH immediately. ROTH can not accept responsibility for goods that were sent back without informing them.
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Page 2: Packing List
PACKING LIST ® 4849.1 ROTIPHORESE PROfessional runVIEW System: 1x runVIEW base station (including power cord) 1x special lid with spectral emission filter and extractor fan within its viewing pane for dyes with green and red fluorescence ® 1x ROTIPHORESE PROfessional III tank w/o lid, with electrodes, 1x 15 x 15 cm gel tray, 2x casting dams 1 pair of 4 mm power output cables 8x double-sided combs:... -
Page 3: Technical Features
METHOD ® ROTIPHORESE PROfessional runVIEW for real-time electrophoresis under blue light ROTIPHORESE ® PROfessional runVIEW is an innovative new system designed for real-time size fractionation and recovery of nucleic acids. You can watch your bands migrate through the gel in blue light –... - Page 4 PROfessional I (Art. No. 2788.1) and ROTIPHORESE PROfessional II (Art. No. 2799.1). No UV safety equipment required for blue light illumination. Convenient power supplies are EV1450 (Art. No. CP56.1) or Roth MINI (Art. No. 2907.1). Operational (runVIEW Base MINI) Wave length (excitation)
- Page 5 If any leakage is seen do not attempt to repair or use the apparatus, but notify Carl Roth GmbH + Co. KG immediately (+49/0721/5606-172).
- Page 6 D. Control interface There are five buttons and four LED indicators on the faceplate. Each LED indicates the activation status or mode of operation of the unit. To select Voltage, Current or Time To switch blue light ON or OFF Increase select Decrease select Start / stop the program...
- Page 7 This will ensure that there is no possibility of gel leakage. 2. Place the gel casting tray on a flat surface or use the Roth levelling table (Art. No. N854.1).
- Page 8 Note: 1.) Blue light bands may only be seen in a darkened room or by shielding the unit from light by a dark cloth or paper. 2.) In order to conserve the blue light lamp, the light will be there for 10 seconds only, and then switch off again by its own.
- Page 9 TIP: For extractions performed with samples of low DNA amount, a small piece of dialysis membrane may be inserted into the extraction wells ahead of elution. The paper may be washed by changing the salt concentration to release the DNA, whereas for the membrane, reversal of the power output cables in the base unit (i.e.
- Page 10 **comb with especially broad wells + narrow marker well (for preparative gels) ® I. Accessories for ROTIPHORESE PROfessional I electrophoresis unit Special lid for runVIEW system, green and red fluorescence 3663.1 Power Supply Roth-MINI 2907.1 Power Supply EV1450 CP56.1 Further accessories see Professional I unit, Art. No. 2788.1. ®...
- Page 11 Gel Running Buffers: ROTIPHORESE - 10 x TBE-Buffer 3061 ROTIPHORESE - 10 x TAE-Buffer T845 ROTIPHORESE - 10 x TAE-Buffer light (for gel elution) 0122 DNA Markers: Please call ++49-0721-5606-0 for our brochure on the complete range of DNA Markers. Staining Reagents: Ethidium bromide solution 1 % 2218...
- Page 12 Problem Cause Solution Check presence of DNA using a UV illuminator or No DNA there methylene blue staining Darken the room or use a dark cloth or paper to shield Too much light present No bands seen in the unit from light. blue light Bands can only be seen through the orange runVIEW Lid not used...
- Page 13 ® Suitable for: Unit PROfessional I 9958.1 Electrophoresis Station ROTIPHORESE PROfessional runVIEW Base MINI 2, ® Suitable for: Unit PROfessional II 9961.1 Carl Roth GmbH+Co. KG Schoemperlenstraße 3-5 76185 Karlsruhe Postfach 100121 76231 Karlsruhe Telefon: (+49)721/5606-0 Telefax: (+49)721/5606-149 E-Mail: info@carlroth.de Internet: www.carlroth.com...
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