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DMT DMT110P-XL Instruction Manual

Myograph system
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MYOGRAPH SYSTEM – 110P-XL
U S E R M A N U A L , V O L . 3 . 7
1

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Summary of Contents for DMT DMT110P-XL

  • Page 1: Trademarks

    MYOGRAPH SYSTEM – 110P-XL U S E R M A N U A L , V O L . 3 . 7...
  • Page 2 Changes may have been made to the software and hardware it describes since then. New information may be supplied separately. This documentation is provided with the DMT Pressure Myograph System – Model 110P-XL All rights reserved. No part of this manual may be reproduced or transmitted in any form or by any means without the written permission of Danish Myo Technology A/S.

  • Page 3: Table Of Contents

    CONTENTS TRADEMARKS SAFETY CHAPTER 1 - SYSTEM OVERVIEW 1.1 INTERFACE 1.2 110P-XL PRESSURE MYOGRAPH CHAMBER CHAPTER 2 – SETTING UP THE 110P-XL SYSTEM 2.1 SETTING UP THE COMPLETE PRESSURE MYOGRAPH SYSTEM 2.2 SETTING UP STEP-BY-STEP 2.3 THE FIRST PRESSURE CALIBRATION 2.4 EXTERNAL PRESSURE MYOGRAPH CONNECTIONS CHAPTER 3 - PRESSURE INTERFACE MENUS 3.1 GENERAL DESCRIPTION OF HOW TO NAVIGATE THE TOUCH SCREEN...
  • Page 4 SAFETY The Pressure Myograph System has been designed for use only in teaching and research applications. It is not intended for clinical or critical life-care use and should never be used for these purposes. Nor for the prevention, diagnosis, curing, treatment, or alleviation of disease, injury, or handicap. CAUTION: •...
  • Page 5 EC DECLARATION OF CONFORMITY DMT A/S Certify and declare that the following apparatus: Pressure Myograph System - DMT110P-XL Restrictive use: Only for laboratory use. Manufactured by: DMT A/S Rho 14 8382Hinnerup Denmark Conforms with the essential requirements of the EMC Directive 2004/108/EC.

  • Page 6: Chapter 1 - System Overview

    CHAPTER 1 - SYSTEM OVERVIEW 1.1 INTERFACE Touch display Power indicator 2x500ml Schott Duran bottles Figure 1.1 Pressure Interface - front...

  • Page 7: 110P-Xl Pressure Myograph Chamber

    Ground connection ON/OFF SWITCH Connection port Inlet for for flowmeter oxygen/carbogen to pressure bottles 24 DC port for power (Minimum 0.5bar and max 1bar) Connection port for pH Connection port for USB connection port electrode (optional) Myograph chamber for computer Analogue recorder output - connection to external data acquisition...
  • Page 8 1.2 110P-XL PRESSURE MYOGRAPH CHAMBER Positioner for horizontal Positioner for vertical adjustment of Glass Cannula Outlet pipe for P2 cannula Adjustment of Mounting cannula Inlet pipe for P1 cannula 25 pin serial port for Communication with Pressure Interface P2 cannula P1 cannula 3-way inlet valve Inlet pressure...

  • Page 9: Chapter 2 - Setting Up The 110P-Xl System

    CHAPTER 2 – SETTING UP THE 110P-XL SYSTEM 2.1 SETTING UP THE COMPLETE PRESSURE MYOGRAPH SYSTEM Figure 2.1 is an example of a complete set-up for the Pressure Myograph System. A detailed step-by-step description is provided in the remaining sections of chapter 2. Figure 2.1 Pressure Myograph –...

  • Page 10: Setting Up Step-By-Step

    Objectives for the 110P-XL chamber will typically be 1.25x and up to max 5x objectives. A camera c-mount is needed for the DMT USB camera. Contact the DMT Sales Department for further product information and price on any optional equipment, including microscopes.
  • Page 11 4. Microscope/camera to computer connection: A. DMT Inverted Microscope: Inside the DMT Microscope there is a USB camera wired to a USB cable and one USB cable to power the microscope light. Connect the two USB cables from the DMT Microscope to the computer USB ports.

  • Page 12: The First Pressure Calibration

    However, in order to ensure that the Pressure Myograph System is working at the highest performance, DMT recommends that a new pressure calibration is performed before the first use. The pressure calibration procedure is described in detail in chapter 3.7.1.
  • Page 13 Figure 2.3 External connections for Pressure Myograph – 110P-XL NOTE: TO FILL THE TUBING AND AVOID AIR BUBBLES, MOUNT THE CALIBRATION SHUNT (RESISTANCE TUBE) AS SHOWN BELOW...
  • Page 14 Calibration shunt tubes delivered in a small box with the system is attached to the two steel pipes Calibration shunt tube connected...
  • Page 15 Fill the P1 Schott bottle with a maximum of 300 ml pre-warmed buffer aerated with carbogen. NOTE: It is very important that the buffer is prewarmed to 37 C and aerated with carbogen to avoid air bubbles in the silicone tubing during an experiment. In the pressure interface menu set the pressure P1 and P2 as follow: P1 = 150 mmHg P2 = 70 mmHg...
  • Page 16 Attach a 20ml syringe here 3. Attach a 20ml syringe to the silicone tubing on the P1 cannula and use the syringe to drag buffer backwards into the P1 cannula and silicone tube. When all air is removed, re-connect the silicone tube from the cannula to the Inlet connecting pipe again replacing the Calibration shunt tube as shown below.
  • Page 17 5. Attach a 20ml syringe to the silicone tube (Shown in the figure below) on the P2 side and use the syringe to drag buffer into the P2 cannula and silicone tube. Attach a 20ml syringe here 6. When all air is removed, re-connect the silicone tube to the Outlet connecting pipe on the 110P-XL chamber, see figure below.
  • Page 18 8. Close the 3-way valves toward the chamber at the P1 and P2 sides. Attach a 20ml here 9. Attach a 20ml syringe at the extra P1 perfusion Inlet and drag a small volume of buffer through the 3-way valve to remove air in the valve. Then close the 3-way valve toward the P1 buffer flask on the interface as shown below.
  • Page 19 2.4.2 EXTRA PERFUSION INLET/OUTLET The extra perfusion inlet/outlet 3-way valves on the Pressure Myograph allow the introduction of special agents or reagents to the perfusion circuit during an experiment. They are placed at P1 and P2 on the Pressure Myograph and the small handle on top of the valves controls the flow d irection. See figure 2.4. Figure 2.4 3-way valve flow settings at P1.

  • Page 20: Chapter 3 - Pressure Interface Menus

    CHAPTER 3 - PRESSURE INTERFACE MENUS This chapter contains a detailed description of how to navigate the touch screen menus and how to use the special features of the Pressure Interface. 3.1 GENERAL DESCRIPTION OF HOW TO NAVIGATE THE TOUCH SCREEN Menus on the Pressure Interface are all accessible by a touch screen.

  • Page 21: Power-Up Screen

    3.2 POWER-UP SCREEN After turning on the Pressure Interface, an introduction screen appears. It displays the software version number. While this screen is displayed the system is auto-initializing. After a few seconds, the Main Menu display will appear, showing the firmware version of the system. 3.3 MAIN MENU The Main Menu gives a good overall picture of how the Pressure Interface is working.
  • Page 22 Buzzer icon: This icon indicates the status on the buzzer. If the icon is grey the buzzer is OFF. If the icon is green the buzzer is ON (active) and will make a sound when the timer reaches zero. Timer: The Timer is a countdown timer that can be set to maximum 00:00:00 24 hours.

  • Page 23: Pressure Menu

    Pressing ENTER will save the new setpoint. NOTE: IT IS NOT POSSIBLE TO CREATE FLOW IN THE 110P-XL SYSTEM THAT WILL CORRELATED WITH THE P1 AND P2 PRESSURE DIFFERENCE. DMT RECOMMENDS THAT NO FLOW IS ALWAYS SELECTED DURING AN EXPERIMENT. IN NO FLOW THE MOUNTED ARTERY WILL BE EXPOSED TO THE P1 PRESSURE ONLY.
  • Page 24 PRESSURE SET P1 Inlet setpoint: +120.0mmHg SELECT P2 Outlet setpoint: +85.0mmHg SELECT Pressure gradient: 5.0mmHg/sec. SELECT Pressure: Flow: ENTER DEFAULT IMPORTANT: • P1 INLET SET POINT CANNOT BE LOWER THAN P2 OUTLET SET POINT. THIS IS TO PREVENT BACKFLOW IN THE SYSTEM. •...

  • Page 25: Heat Menu

    In Main Menu the flow icon also turns green when flow is on. Turning flow on will demand that the P1 is equal or higher than P2 pressure set point. The pressure interface will automatically make the correction of P1 set to the same value as P2 if P1 is lower than P2. Turn flow OFF to simulate a “blind sac”...

  • Page 26: Timer And Buzzer Menu

    3.6 TIMER AND BUZZER MENU The timer and buzzer are controlled from this menu. Use SELECT to program the timer. The timer can be programmed in intervals from 0 to 23:59:59 (Hours: minutes: seconds). When Set Timer is selected press the UP and DOWN arrows to program the timer.

  • Page 27: Setting Menu

    3.7 SETTING MENU The Settings Menu for the 114P system contains several sub- menus. These sub- menus include: • Pressure Calibration • Temperature difference • Record Out Select • Factory diagnostics The Settings Menu for the 110P-XL with pH electrode module •...
  • Page 28 PRESSURE CALIBRATION PROCEDURE FOR PRESSURE MYOGRAPH 110P-XL Figure 3.3 Illustration of proper set up for pressure calibration. 1. Connect the Pressure Interface with the Pressure Myograph chamber. 2. Connect the Big Ben Pressure Manometer to the 3-way valve at P1 on the Pressure Myograph as illustrated in figure 3.3.
  • Page 29 Calibration shunt tubes delivered in a small box with the system is attached to the two steel pipes Calibration shunt tube connected Figure 3.4 Mounting of calibration shunt (resistance tube) in the 110P-XL chamber...
  • Page 30 6. Close the P1 3-way valve towards the P1 bottle on the Pressure Interface, see figure 3.5 normal inlet way, see figure 3.5. The inlet to P1 now comes from the pressure manometer and the Pressure Myograph system is now ready for a calibration procedure. Connected to the pressure manometer Figure 3.5 Three-way valve flow setting at P1 3.7.1.2 Set-up the pressure calibration on the Pressure Interface for the 110P-XL System...
  • Page 31 Step 3 - Apply a pressure of 125mmHg to the Pressure Myograph using the Big Ben Pressure Manometer. When the 3 blue lines of relative values stabilize, go to NEXT STEP. The relative values should be between 3900 to 4500. Step 4 - Apply a pressure of 200mmHg to the Pressure Myograph using the Big Ben Pressure Manometer.
  • Page 32 PH CALIBRATION PROCEDURE (OPTIONAL) The pH Module menu in the 110P-XL is only visible for users that have bought a pH electrode and pH module at DMT. By ordering the pH sensor from DMT the pH sensor is delivered with the unlocking code and a quick guide how to do it.
  • Page 33 The pH calibration procedure is listed in 4 individual steps and needs to be performed one at a time. NOTE: BEFORE THE PH CALIBRATION IS PERFORMED BE SURE TO SELECT THE WAY THE PH ELECTRODE IS TO BE USED. GO TO THE PH SET-UP MENU UNDER INTERFACE SETTINGS The pH calibration procedure is listed in 4 individual steps and needs to be performed one at a time.
  • Page 34 Step 4 - The calibration is now finished. The values in the two bottom lines are the actual pH and temperature reading. 3.7.5.1 pH Set-up Menu Before the pH calibration is performed be sure to select the way the pH electrode is to be used.
  • Page 35 SELECT RECORD OUT SELECT (OPTIONAL) 3.7.4 The Record Select Out Menu determines which data is sent to the BNC analog outputs (REC 1, REC 2 REC 3, REC 4) at the back of the Pressure Interface, four in total. Each output is individually programmable.
  • Page 36 3.7.5.2 Factory Diagnostics Entering Factory Diagnostics will display the Login code to diagnostics window. This window is for trained technicians and is used for diagnostics and troubleshooting purposes. The general user will not have access to this window. However, entering the proper five-digit login code, will give the trained technician access to diagnostics panels that will provide information during a malfunction, or mechanisms to change other settings controlled by the on-board computer.

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