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LS-450 Series Blue LED Pulsed Light Sources
LS-450 Series Blue LED Pulsed Light Sources
The LS-450 Series Blue LED Pulsed Light Sources are compact, low-cost light-emitting diodes that produce
pulsed or continuous spectral output at 470 nm – the blue region – for high-sensitivity emission fluorescence
measurements.
The LS-450 light source can be combined with other sampling optics for a variety of fluorescence applications.
The LS-450 series consists of the following light sources:
•
LS-450 (stand-alone version)
•
R-LS-450 (rack-mount version)
•
R-LS-450-2 (rack-mount version with 2 LEDs)
•
USB-LS-450 (connects directly to USB spectrometers, documented separately)
LS-450 Stand-alone Version
Included Materials
•
LS-450 light source
•
DB-15 accessory cable
•
Power supply
Connecting the LS-450
To connect the LS-450, follow the steps below:
1. Plug the wall transformer into a standard 110V outlet.
2. Connect the power cord to the power input on the LS-
450.
3. Connect an optical fiber to the SMA 905 connector on
the LS-450.
4. Connect the other end of the optical fiber to the SMA
905 connector on your spectrometer or sampling device.
Operation
To use the LS-450, connect the light source by following the instructions above, then proceed:
Using Continuous Mode
To operate the LS-450 in Continuous mode, follow the steps below:
1. Move the switch on the back of the LS-450 to the "contin" setting. This means that the light coming out of
the LS-450 is continuous.
2. Move the switch on the back of the LS-450 to the "off" setting to turn the lamp off.
LS-450 Series Blue LED Pulsed Light Sources
LS-450 Blue LED Pulsed Light Source -
Stand-alone Version
1
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Related Manuals for Ocean Optics LS-450 Series
Summary of Contents for Ocean Optics LS-450 Series
- Page 1 LS-450 Series Blue LED Pulsed Light Sources LS-450 Series Blue LED Pulsed Light Sources The LS-450 Series Blue LED Pulsed Light Sources are compact, low-cost light-emitting diodes that produce pulsed or continuous spectral output at 470 nm – the blue region – for high-sensitivity emission fluorescence measurements.
- Page 2 LS-450 Series Blue LED Pulsed Light Sources Using Pulsed Mode To operate the LS-450 in Pulsed mode, follow the steps below: 1. Connect one end of the DB-15 accessory connector to the back of the LS-450. 2. Connect the other end of the DB-15 accessory connector to the 15-pin connector or adapter on your spectrometer.
- Page 3 LS-450 Series Blue LED Pulsed Light Sources 4. Ensure that there are a constant number of flashes for every integration time by adjusting the integration time in OOIBase to a figure in the table below. ADC2000-PCI ADC1000 S2000 JP3 Pin Row Acceptable Int.
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Page 4: Operation
LS-450 Series Blue LED Pulsed Light Sources R-LS-450 Rack-mount Blue LED Pulsed Light Sources The R-LS-450 Blue LED Pulsed Light Source is the rack- mount version of the LS-450. You can configure the R-LS- 450 to operate in continuous wave mode via manual operation as well as through the software. - Page 5 LS-450 Series Blue LED Pulsed Light Sources • Jumping the CW pins makes the R-LS-450 operate continuously. This means that there is no pulsing of the light source. Other jumper blocks must be configured correctly for this setting to work properly.
- Page 6 LS-450 Series Blue LED Pulsed Light Sources Using Jumper Block 3 (JP3) on the S2000 You can also configure functionality on the R-LS-450 through JP3 on the S2000 circuit board (if you are using the S2000 spectrometer). JP3 on the S2000 spectrometer allows you to control the R-LS-450 through the OOIBase32 software.
- Page 7 LS-450 Series Blue LED Pulsed Light Sources Setting the Integration Time with the R-LS-450 and the S2000 When using any of the pulsed modes and the R-LS-450, you must ensure that a constant number of flashes occur for every integration cycle to ensure a continuous and stable signal. Set the integration time in the OOIBase32 software.
- Page 8 LS-450 Series Blue LED Pulsed Light Sources USB-LS-450 Blue LED Pulsed Light Source Functionally similar to the LS-450, the USB-LS-450 attaches directly to the 10-pin accessory connector of the USB2000 Spectrometer. Its small-footprint design combines with the portability of the USB2000 to form a complete, self-contained light source and spectrometer combination.
- Page 9 LS-450 Series Blue LED Pulsed Light Sources USB-LS-450 Specifications USB-LS-450 LED Specifications Item Specification Minimum of 60 µW into a 600 µm optical fiber Power output: LED drive current: 20 mA +/- 0.15 mA Maximum modulation frequency: 1 kHz 0.5% stability time:...
- Page 10 LS-450 Series Blue LED Pulsed Light Sources LS-450 Series Technical Specifications Lamp Specifications LS-450, R-LS-450, R-LS-450-2 USB-LS-450 Physical dimensions: 90 mm x 50 mm x 32 mm 89 mm x 102 mm x 38 mm (LS-450 only) Power consumption: 25 mA @ 12 VDC 60 mA @ 5 VDC 50 µW into 600 µm fiber...
- Page 11 LS-450 Series Blue LED Pulsed Light Sources Bulb Replacement for LS-450 Series Light Sources The LS-450 and R-LS-450 light sources come with a standard LED that emits blue light at approximately 450-470 nm (see Absolute Spectral Output graphic above). However, the LS-450 and R-LS-450 can accommodate LEDs of different wavelengths, which are sold as accessories to the units.
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Page 12: Troubleshooting
LS-450 Series Blue LED Pulsed Light Sources Changing a Bulb in the R-LS-450 To change the bulb in the R-LS-450, follow the steps below: 1. Ensure that the unit is off and that power is disconnected from the R-LS-450. 2. Use the 1/20 Allen wrench to loosen the small setscrew located on the side of the blue optical housing. If you remove the screw fully, place it in a safe place until you replace it later in this procedure.
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