1. Manuals
  2. Brands
  3. Gow-Mac Manuals
  4. Laboratory Equipment
  5. 400 Series
  6. Operating manual

Gow-Mac 400 Series Operating Manual

Thermal conductivity gas chromatograph
Hide thumbs
Table of Contents

Advertisement

Quick Links

Operating Manual
Series 400/402 & 400-P/402-P
Thermal Conductivity
Gas Chromatograph
Series 400: 120 V, 50/60 Hz
Series 402: 230 V, 50/60 Hz
May 2017
Rev. 7
READ INSTRUCTIONS
BEFORE OPERATING
PO Box 25444, Lehigh Valley, PA 18002-5444 U.S.A. Tel: (610) 954-9000

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the 400 Series and is the answer not in the manual?

Questions and answers

Related Manuals for Gow-Mac 400 Series

Summary of Contents for Gow-Mac 400 Series

  • Page 1 Operating Manual Series 400/402 & 400-P/402-P Thermal Conductivity Gas Chromatograph Series 400: 120 V, 50/60 Hz Series 402: 230 V, 50/60 Hz May 2017 Rev. 7 READ INSTRUCTIONS BEFORE OPERATING PO Box 25444, Lehigh Valley, PA 18002-5444 U.S.A. Tel: (610) 954-9000...
  • Page 2 GOW-MAC Instrument Co. Series 400 / 402 Gas Chromatograph Operating Manual May 2017, Rev. 7...

  • Page 3: Table Of Contents

    Chapter 8 - Experimental Run Through ................41 Chapter 9 - Maintenance ..................... 47 Chapter 10 - Replacement Parts ..................53 Chapter 11 - Mechanical Drawings ..................55 Series 400 / 402 Gas Chromatograph Operating Manual GOW-MAC Instrument Co. May 2017, Rev. 7...
  • Page 4 GOW-MAC Instrument Co. Series 400 / 402 Gas Chromatograph Operating Manual May 2017, Rev. 7...

  • Page 5: Disclaimers

    All information referred to and/or included in this report is current as of the original issue date of this manual. GOW- Instrument Co. makes no warranty or representation with respect to the accuracy of the information or with ® respect to the suitability of the use of such information outside GOW-MAC Instrument Co., nor does GOW-MAC ®...

  • Page 6: Important Information

    Equipment, or from any other use thereof, without the prior written consent of Seller. IMPORTANT INFORMATION These instructions are written for personnel operating the GOW-MAC ® Series 400 Isothermal TCD Gas Chromatograph.

  • Page 7: Warranty

    All requests for service or repair under this warranty must be received within the warranty period by GOW-MAC ® or its authorized representative. All repairs are made at GOW-MAC plants or at the offi ce of authorized representatives. All repairs, adjustments, and other service under this warranty shall be performed free of charge to the purchaser.
  • Page 8 GOW-MAC Instrument Co. Series 400 / 402 Gas Chromatograph Operating Manual May 2017, Rev. 7...

  • Page 9: Important Warning

    PERSONS WHO RELY ON THIS PRODUCT FOR THEIR SAFETY COULD SUSTAIN SEVERE BODILY INJURY OR DEATH. The warranties made by GOW-MAC Instrument Co. with respect to the product are voided if the product is not installed, used and serviced in accordance with the instructions in this manual.

  • Page 10: Important: Gas Flow Adjustment Note

    fi laments! Failure to set fl ow rates on both sides causes oxidation and destruction of the fi laments in the presence of oxygen. GOW-MAC Instrument Co. Series 400 / 402 Gas Chromatograph Operating Manual May 2017, Rev. 7...

  • Page 11: Chapter 1 - Safety Precautions

    D. Store cylinders in a well ventilated area away from heat or ignition sources. E. When installing tubing, provide ONLY approved, adequate pressure reducing regulators and pressure relief devices to prevent over-pressurizing of tubing and equipment. Series 400 / 402 Gas Chromatograph Operating Manual GOW-MAC Instrument Co. May 2017, Rev. 7...
  • Page 12 D. Keep combustibles away from gas cylinders and elimi nate ignition sources. E. DO NOT place papers, charts, samples, etc. on top of the GC. F. Maintain adequate ventilation. G. Dispose of wastes properly. GOW-MAC Instrument Co. Series 400 / 402 Gas Chromatograph Operating Manual May 2017, Rev. 7...

  • Page 13: Chapter 2 - Specifi Cations

    Oven Fittings Accommodates 1/8" or 1/4" o.d. metal or glass columns Oven Capacity Two 1/4" o.d. x 12' columns or correspondingly longer lengths of 1/8" o.d. Series 400 / 402 Gas Chromatograph Operating Manual GOW-MAC Instrument Co. May 2017, Rev. 7...
  • Page 14 Thermal Conductivity Bridge Control Polarity Switch Bridge Zero Adjust Current Adjustment: continuous adjustment to 300 mA (using He carrier) Attenuator: 12 positions to 1024 plus infi nity () GOW-MAC Instrument Co. Series 400 / 402 Gas Chromatograph Operating Manual May 2017, Rev. 7...

  • Page 15: Chapter 3 - Installation

    GOW-MAC does not supply fi eld service. All repairs are made at our Bethlehem, PA facility or by an authorized GOW-MAC representative.
  • Page 16 The ac power cord for the Series 400 is terminated with a straight-blade 3-prong plug rated for 10 amp service that requires a matching receptacle. A cord, with no plug, is supplied with the Series 402 GC. GOW-MAC Instrument Co. Series 400 / 402 Gas Chromatograph Operating Manual May 2017, Rev. 7...
  • Page 17 Follow the recorder NOTE manufacturer's instructions for grounding the recorder. Series 400 / 402 Gas Chromatograph Operating Manual GOW-MAC Instrument Co. May 2017, Rev. 7...
  • Page 18 A. A 1/8" o.d. stainless steel tube extends from the rear of the GC (Figure 3-2). This is the CARRIER GAS INLET. B. Either using the GOW-MAC Installation Kit (Part No. 59-400) or fi ttings and clean copper tubing of your own, connect a 1/8" o.d. piece of copper tubing from the gas outlet located on the gas regulator of the gas cylinder to the CARRIER GAS INLET on the rear of the GC.
  • Page 19 ® 8. Adjust the PRESSURE REGULATOR on the cylinder to a gauge pressure of approximately 30 psig, then shut off the gas at the cylinder. Series 400 / 402 Gas Chromatograph Operating Manual GOW-MAC Instrument Co. May 2017, Rev. 7...
  • Page 20 15 to 20 minutes, there is a leak in the system. 10. The easiest way to locate leaks in the system is through the use of a GOW-MAC Gas Leak Detector, Series 21-070. If one is not available, the use of a leak testing solution (soap solution) and checking for bubbles may be used.
  • Page 21 B. Change the flowmeter to SIDE "B" OUTLET PORT and adjust column "B" for the same fl ow rate (about 60 mL/min) C. The fl ow rates have now been adjusted. Disconnect the fl owmeter. Series 400 / 402 Gas Chromatograph Operating Manual GOW-MAC Instrument Co. May 2017, Rev. 7...
  • Page 22 _____ Cylinders chained or strapped to wall or bench _____ Regulators set for suggested delivery pressure (20-30 psig) _____ Connecting lines and fi ttings leak checked _____ Flow rates checked and adjusted GOW-MAC Instrument Co. Series 400 / 402 Gas Chromatograph Operating Manual May 2017, Rev. 7...

  • Page 23: Chapter 4 - Operation

    6. Digital Panel Meter 15. Injection Port "B" 7. Selector Buttons Column Oven Lid 8. GC Power Switch Exit Ports A & B 9. Polarity Switch Series 400 / 402 Gas Chromatograph Operating Manual GOW-MAC Instrument Co. May 2017, Rev. 7...
  • Page 24 ZERO CONTROL: Adjusts the detector output level for zeroing the baseline of the recorder. Recorder should be zeroed fi rst with short input. GOW-MAC Instrument Co. Series 400 / 402 Gas Chromatograph Operating Manual May 2017, Rev. 7...
  • Page 25 1/8" tubing can be wound on a mandrel of 4" o.d. Columns of 1/8" o.d. with 1/4" ends can be ordered from GOW-MAC. Figure 4-2 illustrates columns "A" & "B". An appropriate column or bypass must be installed in both "A" and "B" sides or damage will result to the detector.
  • Page 26 5. Set COLUMN TEMPERATURE CONTROL to desired setting. 6. Set DETECTOR TEMPERATURE CONTROL to desired setting. Chart 4-1 illustrates the maximum currents for cell temperatures and carrier gases helium, nitrogen, and argon. GOW-MAC Instrument Co. Series 400 / 402 Gas Chromatograph Operating Manual May 2017, Rev. 7...
  • Page 27 0 - 1 mV is recommended. An adjustable chart drive is also recommended: 1/4, 1/2, 1, 2 in/min. A. Connect the recorder to the GC. Series 400 / 402 Gas Chromatograph Operating Manual GOW-MAC Instrument Co. May 2017, Rev. 7...
  • Page 28 The Series 400 GC may also be used with most commercially available chromatography data software. GOW-MAC off ers a chromatography data software program called "DACS". This software enables the customer to directly control the data coming from the GC on a personal computer.
  • Page 29 F. Elute all samples from the column BEFORE the columns cool down. G. Shut ac power OFF. H. Turn helium or other carrier gas "OFF". Series 400 / 402 Gas Chromatograph Operating Manual GOW-MAC Instrument Co. May 2017, Rev. 7...
  • Page 30 (Chart 4-1). _____ Recording device zeroed. _____ Detector fi laments are ON (CARRIER GAS MUST BE FLOWING THROUGH BOTH SIDES OF THE DETECTOR). GOW-MAC Instrument Co. Series 400 / 402 Gas Chromatograph Operating Manual May 2017, Rev. 7...

  • Page 31: Chapter 5 - Microscale Prep

    Push selector button DETECTOR of the Panel Meter. Push and hold the MOMENTARY SWITCH on the rear of the GC to read the temperature. Momentary Switch Outlet Temperature Control Figure 5-1 Series 400 / 402 Gas Chromatograph Operating Manual GOW-MAC Instrument Co. May 2017, Rev. 7...
  • Page 32 1. Attach the adapter to either column "A" or column "B" exit port of the GC. Which exit port is determined by which column the analysis is being performed on. If not using the adapter supplied in GOW-MAC's Microscale Prep Kit, P/N 59-425, make sure the adapter has 6-32 NPT threads.
  • Page 33 12 mL Centrifuge Tube 100 μL Conical Vial Sample Collects GC Connection Vial, Here Conical Reaction Adapter (Threaded) Cotton Plug Figure 5-2 Microscale Prep Glassware Series 400 / 402 Gas Chromatograph Operating Manual GOW-MAC Instrument Co. May 2017, Rev. 7...
  • Page 34 GOW-MAC Instrument Co. Series 400 / 402 Gas Chromatograph Operating Manual May 2017, Rev. 7...

  • Page 35: Chapter 6 - Valves

    A. All valves must be treated with CARE. Foreign materials such as metal fi lings or abrasive particles can permanently damage the sliders of the valves. GOW-MAC recommends 2-micron particle fi ltration of gases connected to the gas sample valves.
  • Page 36 Other sample loops are available, including: 0.25 mL, 0.50 mL, 1.0 mL, 2.0 mL, 3.0 mL, 4.0 mL, 5.0 mL, 10.0 mL, and 20.0 mL. GOW-MAC Instrument Co. Series 400 / 402 Gas Chromatograph Operating Manual May 2017, Rev. 7...
  • Page 37 C. Connect the Interface Cable provided to the VALVE ACTUATORS Terminal Strip located on the back of the Valve Housing(s). Moving top to bottom along the strip, attach the color coded wires in the following sequence: Series 400 / 402 Gas Chromatograph Operating Manual GOW-MAC Instrument Co. May 2017, Rev. 7...
  • Page 38 Green Brown Yellow Black D. Refer to the External Control Option in the software operating manual for complete installation/operating instructions. GOW-MAC Instrument Co. Series 400 / 402 Gas Chromatograph Operating Manual May 2017, Rev. 7...

  • Page 39: Chapter 7 - Software

    Check the requirements for each device or instrument in their respective manuals. GOW-MAC GCs can be set up many diff erent ways to acquire and control the data produced by the GC. These setups can range from being completely manual, to almost fully automated with the proper devices.
  • Page 40 GOW-MAC Instrument Co. Series 400 / 402 Gas Chromatograph Operating Manual May 2017, Rev. 7...

  • Page 41: Chapter 8 - Experimental Run Through

    fl ow meter at the exit ports.. E. Disconnect fl owmeter. Electrical A. DETECTOR CURRENT SWITCH "OFF". B. BRIDGE CURRENT CONTROL fully counterclockwise (CCW). Series 400 / 402 Gas Chromatograph Operating Manual GOW-MAC Instrument Co. May 2017, Rev. 7...
  • Page 42 C. Draw a 1 μL SAMPLE into the syringe and then draw about 5 μL of air. With the recorder running (1"/min. or faster), inject the sample onto the Carbowax column. GOW-MAC Instrument Co. Series 400 / 402 Gas Chromatograph Operating Manual...
  • Page 43 Figure 8-1 Typical Chromatogram on Carbowax Compound Boiling Point 98 °C n-Heptane Tetrahydrofuran 64 °C 2-Butanone 80 °C 97 °C n-Propanol Table 8-1 Components of Sample Series 400 / 402 Gas Chromatograph Operating Manual GOW-MAC Instrument Co. May 2017, Rev. 7...
  • Page 44 This is important because peaks get wider the longer they stay in the column. One way of expressing n is: retention time n = 16 = 16 peak width GOW-MAC Instrument Co. Series 400 / 402 Gas Chromatograph Operating Manual May 2017, Rev. 7...
  • Page 45 F. Elute all samples from the column BEFORE the columns cool down. G. Shut ac power OFF. H. Turn helium or other carrier gas "OFF". Series 400 / 402 Gas Chromatograph Operating Manual GOW-MAC Instrument Co. May 2017, Rev. 7...
  • Page 46 TURNED OFF BEFORE THE HELIUM IS TURNED OFF. AS CAUTION LONG AS THE CELL AND FILAMENTS ARE HOT, THE HELIUM SHOULD BE FLOWING THOUGH THE FLOW RATE CAN BE REDUCED TO CONSERVE HELIUM. GOW-MAC Instrument Co. Series 400 / 402 Gas Chromatograph Operating Manual May 2017, Rev. 7...

  • Page 47: Chapter 9 - Maintenance

    The Detector and Elements A. Limits of Operation The elements (aka hotwires or fi laments) used in the Series 400 GC are GOW-MAC rhenium-tungsten (WX). They may be purchased from GOW-MAC in "quads". A quad consists of four matched and balanced fi laments with cable (P/N 13-355-400). WX fi...
  • Page 48 H. Remove the two (2) screws from the CELL BRACKET. Remove the two (2) upper screws from the CELL HOUSING. J. CAREFULLY remove the CELL HOUSING. GOW-MAC Instrument Co. Series 400 / 402 Gas Chromatograph Operating Manual May 2017, Rev. 7...
  • Page 49 B. Figure 9-2 shows the injection port for low dead volume utilizing on-column injection. Series 400 / 402 Gas Chromatograph Operating Manual GOW-MAC Instrument Co. May 2017, Rev. 7...
  • Page 50 A. The septums used in the Series 400 GC are standard 9 mm o.d. and may be obtained directly from GOW-MAC (part no. 180-123S). The INJECTION PORT NUT may be easily removed and the septum replaced. The INJECTION PORT NUT acts as a heat sink, and should be kept clean and polished.
  • Page 51 ± 0.25%. An external voltage control provides continue adjustment of the output voltage from 3 to 20 DC. This control is marked CURRENT on the front panel of the GC. Series 400 / 402 Gas Chromatograph Operating Manual GOW-MAC Instrument Co. May 2017, Rev. 7...
  • Page 52 TCDs received without a completed Health & Safety form or RMA will not be permitted into our facility. Display the RMA number on your shipping label to insure prompt attention upon arrival at GOW-MAC. GOW-MAC Instrument Co. Attn: Repair Dept, RMA# _______ 277 Brodhead Road Bethlehem, PA 18017-8600 Please include a contact person, phone number, service required and a P.O.

  • Page 53: Chapter 10 - Replacement Parts

    127-354 Knob, locking type 127-386 Meter, Panel, LED 3 ½ digit digital 128-274 *Potentiometer, 1M (Outlet) 111-105 * Replacement parts for instruments with preparative option Series 400 / 402 Gas Chromatograph Operating Manual GOW-MAC Instrument Co. May 2017, Rev. 7...
  • Page 54 180-123S *Microscale Prep Kit 59-425 (2 prep columns & GC glassware) *Microscale Prep Glassware 180-350 Replacement Kit * Replacement parts for instruments with preparative option GOW-MAC Instrument Co. Series 400 / 402 Gas Chromatograph Operating Manual May 2017, Rev. 7...

  • Page 55: Chapter 11 - Mechanical Drawings

    Wiring Schematic (115 V) Drawing C-18002 Wiring Schematic (230 V) Drawing C-20462 Wiring Schematic 400-P (115 V) Drawing C-18002-1 Wiring Schematic 402-P (230 V) Drawing C-20462-1 Series 400 / 402 Gas Chromatograph Operating Manual GOW-MAC Instrument Co. May 2017, Rev. 7...
  • Page 56 GOW-MAC Instrument Co. Series 400 / 402 Gas Chromatograph Operating Manual May 2017, Rev. 7...
  • Page 57 Series 400 / 402 Gas Chromatograph Operating Manual GOW-MAC Instrument Co. May 2017, Rev. 7...
  • Page 58 GOW-MAC Instrument Co. Series 400 / 402 Gas Chromatograph Operating Manual May 2017, Rev. 7...
  • Page 59 INSTRUMENT CO. .X ±.04 DWG. .XX ±.02 277 BRODHEAD ROAD BETHLEHEM, PA 18017-8600 .XXX ±.010 REV. NO. A-17065 HOLES ±.003 DATE CHANGE ANGULAR ± 1° Series 400 / 402 Gas Chromatograph Operating Manual GOW-MAC Instrument Co. May 2017, Rev. 7...
  • Page 60 GOW-MAC Instrument Co.
  • Page 61 115VAC, 60 HZ HEATERS PRESSURE COLUMN SWITCH OVEN 300 W P/N 120-130 DETECTOR DETECTOR (3) RTD SENSORS 100 W COLUMN DET. INJECTOR INJ. PORT (2) 60 W POWER SWITCH & CIRCUIT BREAKER CASE GND. TO RECORDER CONSTANT CURRENT POWER SUPPLY SHIELD PCB ASSEM.
  • Page 62 GOW-MAC Instrument Co. Series 400 / 402 Gas Chromatograph Operating Manual May 2017, Rev. 7...
  • Page 63 REP-005 Health-Safety Declaration Doc REV 6 (0212).docx Rev. 6 2/15/2012, gsj 277 Brodhead Road, Bethlehem, PA 18017 U.S.A. Tel: (610) 954-9000 Fax: (610) 954-0599 E-mail: sales@gow-mac.com URL: www.gow-mac.com Series 400 / 402 Gas Chromatograph Operating Manual GOW-MAC Instrument Co. May 2017, Rev. 7...
  • Page 64 GOW-MAC Instrument Co. Series 400 / 402 Gas Chromatograph Operating Manual May 2017, Rev. 7...

This manual is also suitable for:

402 series402-p series400-p series

Table of Contents