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D. O
PERATING
Your new QS-710 Quick Screen Horizontal Unit is cleaned and wiped prior to packaging; however,
components should be washed in warm soapy water prior to use in the laboratory. A mild dish wash-
ing liquid, like Joy, works well.
Gently wash the tank, lid, UVT casting tray, and casting fixture in warm soapy water, taking care not
to scratch any of the acrylic components such as the tank and UVT tray. Do NOT wash Power Cords.
NOTE: It is also recommended that the UVT casting tray be cleaned with alcohol prior to use. Be
certain the entire unit is dry prior to use.
P
REPARATION
1.) Select the percentage gel necessary to effectively resolve your sample, use Table 1 as a guide.
2.) Weigh an appropriate quantity of agarose (0.3% means 0.3gm of agarose per 100ml of gel
volume) and place it into a 250ml flask.
3.) Make up 500ml of either 1X TAE or 1X TBE electrophoresis buffer. See below:
Electrophoresis Buffers
The two most commonly used buffers for horizontal electrophoresis of double stranded DNA in
agarose gels are Tris-Acetate-EDTA (TAE) [IB70160] and Tris-Borate-EDTA (TBE) [IB70150].
While the resolving powers of these buffers are very similar, the relative buffer capacities are very
different, conferring different run attributes which are summarized below:
TAE (IB70160): Tris-acetate has traditionally been the more commonly used buffer. However, its
TBE (IB70150): Tris-borate's significantly greater buffering capacity and its relatively low current
4
I
NSTRUCTIONS
O
A
G
F THE
GAROSE
EL
Table 1 Gel Concentrations and Resolving Ranges
Concentration of
Agarose in Gel
(% w/V)
0.3%
0.6%
0.7%
0.9%
1.2%
1.5%
2.0%
* Table taken from Sambrook, J., Fritsch, E.F., & Maniatis, T. (1989)
Molecular Cloning, A Laboratory Manual, 1, 6. 8, 613.
relatively low buffer capacity will become exhausted during extended elec-
trophoresis, making buffer recirculation necessary in runs exceeding 140 mA-
hours. Potential advantages of using TAE buffer over TBE buffer include superi-
or resolution of supercoiled DNA and approximately 10 % faster migration of
double-stranded linear DNA fragments.
draw eliminates the need for recirculation in all but the most extended runs (>
300 mA-hours). TBE buffer systems are not recommended when fragments are to
be recovered from the gel after electrophoresis.
- DNA
Efficient Range of
Separation of Linear DNA
(Kb)
5 - 60
1 - 20
0.8 - 10
0.5 - 7
0.4 - 6
0.2 - 3
0.1 - 2
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