Table of Contents
Advertisement
Quick Links
Advertisement
Table of Contents
Related Manuals for Ibi IB44000
Summary of Contents for Ibi IB44000
- Page 1 OPERATION MANUAL Semi-Dry Blotters 16cm x 16cm | 24cm x 30cm IB44000 | IB45000...
-
Page 3: Important Safety Information
If there is ANY sign of shipping damage, please contact the carrier and file a claim with them immediately. Contact the distributor from which you purchased the item or IBI Scientific directly for assistance at (800) 253-4942 or (563) 690-0484. -
Page 4: Principles Of Operation
The transfer of these components from gels to a solid support requires either an electrical current, vacuum suction or diffusion to move the components from the gel to the solid support. The IBI Semi-Dry Blotting apparatus is a device that can migrate these components from the gel to a solid support by way of electrical current. - Page 5 Several trans units can be stacked on top of each other in order to increase the throughput of the appa- ratus. The IB44000 Semi-Dry unit is designed to accommodate as many as 6 trans units stacked on top of each other and run simultaneously.
- Page 6 • Anode Buffer Solution #1: 0.3M TRIS, 20% methanol, pH 10.4 • Anode Buffer Solution #2: 0.025M TRIS, 20% methanol, pH 10.4 • Cathode Buffer Solution: 0.025M TRIS/0.04M amino caproic acid, 20% methanol, pH 9.4 • IBI TRIS Powder: IB70142(500gm), IB70144(1Kg), IB70145(5Kg) Operational Procedures and Techniques for Western Blotting Warning: Be sure to wear gloves, safety glasses and lab coat/apparel when working with gels, buffers and semi-dry apparatus.
- Page 7 NOTE: The filter pads and blotting paper noted below are 10cm x 10cm. If you need larger you will need to source elsewhere. • DNA is separated on an IBI Molecular Biology agarose gel (0.7 – 1%) poured with 1X TBE buffer or IBI Electrophast buffer. For genomic DNA use 0.7% and for plasmid digest use 0.8% - 1% •...
- Page 8 Operational Procedures and Techniques for Semi-Dry RNA Blotting Denaturing Formaldehyde Agarose gels • RNA is separated on a 1.2% Agarose gel with 1X MOPS and 1.8% formaldehyde. (IB70042 – Molecular Biology Grade Agarose & IB70175 – 10X MOPS w/DEPC water.) •...
-
Page 9: Troubleshooting
Recommended Solutions for DNA / RNA Semi-Dry Transfers DNA Solutions: • Solution 1 Denaturing solution 0.5M NaOF, 1.5M NaCl • Solution 2 Neutralizing solution 1.0M TRIS-Hcl/pH 8.0 1.5M NaCl • Solution 3 1X TBE 0.089M TRIS 0.089M Boric Acid 0.002M EDTA, pH 8.3 10X TBE 108gm TRIS base 55gm Boric acid... -
Page 10: Limited Warranty
Our limited warranty as noted above extends to the direct end user of IBI Scientific products only. This warranty is in lieu of all other warranties whether expressed or implied, including warranties of merchantability or fitness for a particular purpose. In no situation shall IBI Scientific be... - Page 11 IB44000 IB45000...
- Page 12 7445 Chavenelle Road Dubuque, IA 52002 800-253-4942 (563) 690-0484 info@ibisci.com IBISCI.com Rev. 1...