Related Manuals for Ibi QS-710
Summary of Contents for Ibi QS-710
- Page 1 ’ PERATOR ANUAL QS-710 - UICK CREEN ORIZONTAL LECTROPHORESIS CM X IBI Catalog Number: IB51000 IBI SCIENTIFIC SCIENTIFIC...
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Page 2: Table Of Contents
EPARATED RAGMENTS HOICE OF UFFER OLTAGE TAINING OLUTION E. M AINTENANCE OF F. R & A EPLACEMENT ARTS CCESSORIES QS-710 A CCESSORY TEMS AND EPLACEMENT ARTS QS-710 C OMBS G. R IBI P ELATED RODUCTS H. R IBI C 12-14... -
Page 3: Products
AFETY NFORMATION Important Safety Information! Please read this manual carefully before operating your new IBI QS-710 unit. This manual contains important operating and safety information. To best use the product, please read the entire manual carefully prior to use. To avoid possible injury, this product should only be used for its intended purpose. - Page 4 PERATING NSTRUCTIONS Your new QS-710 Quick Screen Horizontal Unit is cleaned and wiped prior to packaging; however, components should be washed in warm soapy water prior to use in the laboratory. A mild dish wash- ing liquid, like Joy, works well.
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Page 5: Preparation Of The
4.) Add ethidium bromide (IB40075) to the diluted electrophoresis buffer to a final concentration of 0.5μg/ml. NOTE: The addition of ethidium bromide to both the gel and the running buffer will result in maximum detection levels by providing high levels of sample fluorescence with an evenly low level of background. -
Page 6: Casting The Gel
The following protocol will make 50ml of a 1.5% agarose gel containing 1X MOPS [3-(N- Morpholino)-Propanesulfonic Acid]-Acetate-EDTA (MAE) buffer and 2.2M formaldehyde, result- ing in a 7.5mm thick gel: 1.) Weigh out 0.5gm of agarose, and place into a 125ml flask. 2.) Add 43.5ml of DEPC (or acetic anhydride) treated water. -
Page 7: Removing The Comb
EMOVING THE 1.) When the gel is solidified and fully opaque, carefully remove the comb with a gentle wiggling, upward motion. If the comb is difficult to remove or if a low percentage gel is being used, overlay the comb area with a small volume of 1X electrophoresis buffer to preserve the integri- ty of the wells. -
Page 8: Sample Electrophoresis
1.) The QS-710 is designed for quick screen electrophoresis. The maximum suggested applied voltage for the electrophoresis of DNA in agarose gels using the QS-710 is 100V. In a 1% TBE gel, this translates into a run time of approximately 1/2 hour. Lower voltages may be used, of course, and as a general rule, a 50V run will take twice as long as a 100V run. -
Page 9: Etection And Choice Of Buffer
2.) Follow the sample migration into the gel using the loading dye as an indicator. (See “Choice of Buffer” for the Sample Loading Buffer recipe) Allow the samples to migrate until the fragments have separated, normally until the bromophenol blue dye front has migrated 3/4 of the way down the gel. -
Page 10: Voltage
8.0 OLTAGE The QS-710 is designed for rapid electrophoresis with moderate resolution. Suggested voltage is 100V for a 30 to 60 minute run. Higher voltages may be used to decrease run time, however, the volt-hours should remain constant. One should not exceed 200 volt-hours without changing the buffer in the unit. -
Page 11: Maintenance Of
Replacement Bubble Level IB50505 Replacement Tank Connector Kit IB51020 QS-710 UV Transmittant Casting Tray IB51025 QS-710 Casting Fixture IB51030 QS-710 UVT Casting Tray with Casting Fixture IB51200 Replacement QS-710 Buffer Tank IB51300 Replacement QS-710 Lid IB51305 Replacement QS-710 Electrode Assembly... -
Page 12: Qs-710 Combs
0.8mm by 12-tooth combs, power cords, and manual SH-300 IBI 300V Power Supply (300V / 400mA / 120W) The SH-300 has constant voltage or constant current capability, memory settings, and a LED display. Comes complete with power supply, 120V grounded power cord, and manual. - Page 13 H. R IBI C ELATED ERTIFIED EAGENTS IB01010 6X Loading Dye IB01015 5X RNA Gel Loading Dye Kit 100RxN IB01020 10X TBE Pouch 1 Pouch IB01030 25X Tris-Acetate EDTA Buffer Pouch 1 Pouch IB74020 Acridine Orange 25gm IB70016 Acrylamide:Bisacrylamide, 29:1...
- Page 14 IB21040 Dithiothreitol (DTT) IB21045 Dithiothreitol (DTT) 25gm IB70180 EDTA, disodium salt 100gm IB70182 EDTA, disodium salt 500gm IB70184 EDTA Solution (0.5M), pH 8 100ml IB70185 EDTA Solution (0.5M), pH 8 4x100ml IB40060 Ethidium Bromide IB40075 Ethidium Bromide Solution, 10mg/mL 10ml IB72028 Formamide, ACS Grade 500ml...
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Page 15: References
IBI Scientific to the customer, whether based on contract, tort, or any other legal theory. In no such event shall IBI Scientific be liable for dam- ages which exceed the purchase price of any products.