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Allevi 1 User Manual

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Allevi 1
User Guide – 1.0.6
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Summary of Contents for Allevi Allevi 1

  • Page 1 Allevi 1 User Guide – 1.0.6...
  • Page 2 B U I L D W I T H L I F E...

  • Page 3: Table Of Contents

    Table of Contents WELCOME PECIFICATIONS AFETY AND OMPLIANCE GETTING STARTED ’ S IN THE LLEVI IAGRAM ETTING UP YOUR RINTER ETTING UP YOUR OFTWARE START PRINTING IRST IOPRINT MAINTENANCE & TROUBLESHOOTING ROUBLESHOOTING AND ONTACT...

  • Page 4: Welcome

    Supported File Types: STL, gcode Operating System: Windows, MacOSX, Linux WARNING: The Allevi 1 includes moving parts that can cause injury. Never reach inside the printer while it is in operation. WARNING: Do not leave the printer unattended while it is in operation.

  • Page 5: Getting Started

    Let’s Get Started!

  • Page 6: Whats In The Box

    WHAT’S IN THE BOX Power cord Allevi 1 bioprinter Air compressor* Compressor hose Starter bioink kit...

  • Page 7: Allevi 1 Diagram

    ALLEVI 1 DIAGRAM Air Compressor Line CORE™ printhead Autocalibration pad Build Plate Power On/Off...

  • Page 8: Setting Up Your Printer

    SETTING UP YOUR PRINTER 1. Carefully remove your printer from the packaging by grasping the printer frame firmly with both hands. 2. Use the power cable to connect the printer to a power supply via the port located on the right side of the printer (labelled Step 2 below). 3.

  • Page 9: Setting Up Your Software

    SETTING UP YOUR SOFTWARE You can also follow a video at allevi3d.com/getting-started 1. Go to bioprint.allevi3d.com. Our software runs out of a browser and requires no downloads! We recommend Google Chrome or Firefox, but Safari and Edge are also acceptable. 2.
  • Page 10 4. Type in your device serial number and click ‘Add printer.’ Your serial number can be found on the back of your printer or in your onboarding email from the Allevi Support Team. 5. Once your printer is registered, it will appear as an available printer in your printer list.
  • Page 11 6. To connect your Allevi bioprinter to the Internet, open your WiFi settings, and look for a network called “Allevi WiFi Connect.” After connecting to it, the WiFi Dashboard should pop up automatically. If it does not, go to 192.168.42.1 in your browser.

  • Page 12: Start Printing

    Let’s Start Printing!

  • Page 13: Your First Bioprint

    GETTING STARTED Your First Bioprint The Allevi Bioprint Online software is the most comprehensive and versatile bioprinting software. We recommend watching this introduction video. If you prefer watching videos, visit allevi3d.com/getting-started and watch the ‘Your First Bioprint’ video. 1. Turn on your printer. Yellow lights will stay on while the printer loads its firmware and connects to the Internet.
  • Page 14 3. This will bring you to your printer Status section. Here you can get an overview of your print settings. We recommend always autocalibrating first. Always autocalibrate with a syringe and needle loaded in the printhead. Do NOT autocalibrate with a dish on the bedplate. 4.
  • Page 15 5. Select your print file. 6. Select your wellplate. For your first bioprint, we’ll be using a petri dish.
  • Page 16 7. If you haven’t already autocalibrated, you can here. You also manually calibrate. a) To manually calibrate the printer with the manual controls: i. First, autocalibrate with a syringe loaded. ii. Press the button to center the extruder over your chosen build plate. iii.
  • Page 17 For Pluronic, set the pressure to 80 PSI. Leave it at room temperature. 9. For photocurable bioinks, the Allevi 1 offers both UV and visible light crosslinking, which can be changed using the UV/Visible toggle. There are three main settings for crosslinking: Always On, During Print, and Post Print.
  • Page 18 10. Once the everything is ready to go, ‘Print’ to start building with life! 11. Sit back and watch your printer go!

  • Page 19: Maintenance & Troubleshooting

    When you buy an Allevi bioprinter, you’re not just getting a biofabrication platform; you’re gaining the Allevi Advantage, which is all the experience and expertise Allevi has to offer. One very useful resource is Allevi’s Build With Life repository. We upload protocols, experiments, and guides to help you be more successful with bioprinting.
  • Page 20 Allevi, Inc | 3401 Grays Ferry Ave | Building 176 | Philadelphia PA | 19146 Copyright Allevi, Inc 2018...

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