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Summary of Contents for Allevi 3
- Page 1 Allevi 3 User Guide – 1.0.3...
- Page 2 B U I L D W I T H L I F E...
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Page 3: Table Of Contents
Table of Contents WELCOME PECIFICATIONS AFETY AND OMPLIANCE GETTING STARTED ’ S IN THE LLEVI IAGRAM ETTING UP YOUR PRINTER ETTING UP YOUR SOFTWARE START PRINTING LLEVI OFTWARE ROJECT ORKFLOW REPARING YOUR IOPRINTING IN THE EVICE TAB MAINTENANCE & TROUBLESHOOTING ROUBLESHOOTING AND ONTACT... -
Page 4: Welcome
This User Manual will help get you started using your Allevi bioprinter. In the first section of this guide you will learn about the basic components of your Allevi, how to set it up, download the correct software, and load bioinks. -
Page 5: Specifications
Supported File Types: STL, gcode Operating System: Windows, MacOSX, Linux WARNING: The Allevi 3 includes moving parts that can cause injury. Never reach inside the printer while it is in operation. WARNING: Do not leave the printer unattended while it is in operation. -
Page 6: Getting Started
Let’s Get Started! -
Page 7: What's In The Box
WHAT’S IN THE BOX Power cord Allevi 3 bioprinter Starter bioink kit Safety glasses Air compressor* Compressor hose *Air compressor arrives separately... -
Page 8: Allevi 3 Diagram
ALLEVI 3 DIAGRAM Air Compressor Line Swappable Extruder (heating and cooling) Autocalibration pad Build Plate Power On/Off... -
Page 9: Setting Up Your Printer
(labelled Step 2). 3. Power on the printer by pressing the power button located front of the printer. 4. Attach the air compressor to the printer by connecting the compressor hose to nozzle on the left side of the printer (labelled Step 4). -
Page 10: Setting Up Your Software
We recommend Google Chrome or Firefox, but any of the following browsers are also acceptable: Safari, Opera, Edge, or Internet Explorer 2. To set up an account, fill out the form and click ‘Register.’ 3. To register your printer, go to http://bioprintallevi.com/devices. Click ‘Register.’... - Page 11 4. Next, type in your device number and click ‘Register’ again. Your device number can be found in your onboarding email sent to you by the Allevi Support Team. 5. Once your printer is registered, it will appear as an available printer.
- Page 12 6. To connect your Allevi bioprinter to the Internet, open your WiFi settings, and look for a network called “WiFi Connect.” Connect to it, then go to the Offline Dashboard at http://192.168.42.1:8007. By connecting to this, your computer will briefly lose access to the Internet.
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Page 13: Start Printing
Let’s Start Printing! - Page 14 INTRODUCTION TO THE ALLEVI SOFTWARE The Allevi software is designed with the 3D printing scientist in mind. It houses the various projects you’re working on and makes them easily accessible. In a project you can store data, notes, pictures, and prints.
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Page 15: Preparing Your Stl
To begin, we will use a predesigned STL file. The Allevi software project workflow incorporates a built-in slicer. However, for now, we will use Repetier-Host as our slicing software. Repetier-Host is a third-party software that converts STL files (3D models) into gcode files (instruction the printer can read). - Page 16 ‘Connect.’ 3. Load your syringe with Pluronic F127. Visit the Allevi Protocols page to watch a video on how to load syringes for your Allevi 3. We suggest filling your syringe with about 3 ml of material for your first print: a) Attach a 30 gauge 0.25”...
- Page 17 4. Select your wellplate and then activate your extruders by clicking on the red extruder button. 7. Now that you’ve activated your extruder, you can test its extrusion, move it around (in the ‘Jog Panel’), or calibrate it. a. For autocalibration, click the ‘Autocalibrate’ button. (Autocalibration takes ~2 minutes and will avoid the need for the ‘Jog Panel’.) For short needles, only add the wellplate after autocalibration is complete, otherwise the extruder will crash.
- Page 18 b. To manually calibrate the printer, use the Jog Panel. i. First, home the Z axis, followed by the X/Y axis. ii. Using the X and Y arrows, move the loaded extruder to over where you want to print. If it’s for a wellplate, calibrate in the upper left well. We recommend using the 20mm step size.
- Page 19 5. For photocurable bioinks, the Allevi 3 offers both UV and visible light crosslinking, which can be changed using the UV/Visible toggle. There are three main settings for crosslinking: Always On, During Print, and Post Print. They use variables such as light intensity, layer frequency, and duration.
- Page 20 7. Once the wellplate, calibration, and gcode file are all ready, you can press ‘Start Print’ to start building with life! a) Note: As the printer loads the gcode, the printer’s yellow lights will turn on. 8. Sit back and watch your printer go!
- Page 21 TROUBLESHOOTING AND MORE INFORMATION When you buy an Allevi bioprinter, you’re not just getting a biofabrication platform; you’re gaining the Allevi Advantage, which is all the experience and expertise Allevi has to offer. One very useful resource is Allevi’s Build With Life repository. We upload protocols, experiments, and guides to help you be more successful with bioprinting.
- Page 22 Allevi, Inc | 3401 Grays Ferry Ave | Building 176 | Philadelphia PA | 19146 Copyright Allevi, Inc 2018...
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