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Allevi 2 User Manual

Bioprinter
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Allevi 1
User Guide
Allevi 2
User Guide – 1.6.0
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Summary of Contents for Allevi 2

  • Page 1 Allevi 1 User Guide Allevi 2 User Guide – 1.6.0...
  • Page 2 B U I L D W I T H L I F E...

  • Page 3: Table Of Contents

    Table of Contents WELCOME PECIFICATIONS AFETY AND OMPLIANCE GETTING STARTED ’ S IN THE LLEVI IAGRAM ETTING UP YOUR PRINTER ETTING UP YOUR SOFTWARE YOUR FIRST BIOPRINTS RINTING INGLE ATERIAL TRUCTURES RINTING ATERIAL TRUCTURES MAINTENANCE & TROUBLESHOOTING ESETTING ASSWORDS ROUBLESHOOTING AND ONTACT...

  • Page 4: Welcome

    This User Manual will help get you started using your Allevi bioprinter. In the first section of this guide you will learn about the basic components of your Allevi, how to set it up, download the correct software, and load bioinks.

  • Page 5: Specifications

    Supported File Types: gcode Operating System: Windows, Mac OSX, Linux WARNING: The Allevi 2 includes moving parts that can cause injury. Never reach inside the printer while it is in operation. WARNING: Do not leave the printer unattended while it is in operation.

  • Page 6: Getting Started

    Let’s Get Started!

  • Page 7: What's In The Box

    WHAT’S IN THE BOX Allevi 2 Printer Safety Goggles USB Cable Power Adapter Starter Bioink Kit Compressor Hose Air Compressor* *Air compressor arrives separately...

  • Page 8: Allevi 2 Diagram

    ALLEVI 2 DIAGRAM Air Compressor Lines Dual Extruder (Left: Extruder 1, Right: Extruder 2) Build Plate Power On/Off...

  • Page 9: Setting Up Your Printer

    1. Carefully remove your printer from the packaging by grasping the printer frame firmly with both hands. 2. Use the power cable to connect the printer to a power supply via the port located on the right side of the printer (labelled Step 2).

  • Page 10: Setting Up Your Software

    FTDI Drivers b. MacOSX: FTDI Drivers. 2. Download the installer for your operating system from the Allevi Software page. Double-click desktop application to run Bioprint. 4. In the setup window, create a username and password to use with Bioprint and then proceed to register your printer.
  • Page 11 7. Install Repetier Host. Close window if Repetier-Host Server opens in your browser and continue. 8. Open Repetier-Host. Go to “Config” > “Printer Settings” and assign the following values. 9. Download the Slic3r Config Bundle file. 10. In Repetier Host, go to the Slicer tab and click “Configuration”...
  • Page 12 11. Go to “File” > “Load Config Bundle…” and select the Slic3r Config Bundle file you just downloaded. Troubleshoot: Sometimes when you type in bioprint/ or bioprint:9999/ on the web browser nothing appears. When this happens open command prompt in the start menu. In command prompt type “ipconfig”, Find the IPv4 number.

  • Page 13: Your First Bioprints

    Let’s Start Printing!
  • Page 14 Save, copy, or scale object with the items on the right. c. Assign the STL to an extruder by using with the extruder drop down menu. 2. Enter your print settings. a. Select the “Slicer” tab to assign preconfigured Slic3r settings to slice the file.
  • Page 15 Recommendation: 2-6 mm/s is good for a small accurate design (example: lattice). 8-16 mm/s is good for a large design (example: ear). Speed is a print parameter that is very dependent on...
  • Page 16 c. Nozzle Diameter – Enter the number that accurately depicts your nozzle tip inner diameter NOTE: Your layer height must be less than your nozzle diameter to correctly slice NOTE: Once the changes have been made, remember to save and label your settings. 4.
  • Page 17 Printing One STL 1. Navigate to the Bioprint Window and log in. 2. Navigate to the Bioprint window and Upload the G-code file. NOTE: You can sort your gcode files by name, date, or size by clicking the wrench.
  • Page 18 3. Connect your printer to your computer. 4. First, home the Z-axis. 5. Second, home the E-axis.
  • Page 19 6. Third, home the X/Y-axis. 7. Use the dropdown menu to select “Petri Dish.” Click the button “Select Well Plate.” NOTE: If you want to print in multiple wells, all you need to do is select that option. Our software will automatically replicate your single gcode file across all the wells.
  • Page 20 NOTE: Bioinks will be loaded differently depending on the type of material it is. Watch this video to learn how to prepare Pluronic to print with your Allevi 2. We suggest filling your syringe with about 3 ml of material for your first print.
  • Page 21 b. Adjust pressure between approximately 30-50 PSI for pluronic until material extrudes continuously. Press extrude again to stop the flow. 3. Switch step size from 10mm to 1mm. Then use up and down buttons to move the bed plate (Z) closer to the extruder (Extruder 1) to the point where the dish is almost touching the needle.
  • Page 22 NOTE: You must always calibrate Extruder 1 first and then Extruder 2. If you only plan to use Extruder 1, you can skip calibrating Extruder 2. However, if you only want to use Extruder 2, you can calibrate Extruder 1 to any position. However, you must still press...
  • Page 23 6. Now that your tool is calibrated, you are ready to print!! When ready, click ‘Load and Print.’ 7. Sit back and marvel at your awesome, first bioprint!
  • Page 24 Slicing Two STL Designs FOR WINDOWS: 1. Import two STL designs and click “Center Object” to align them both. 2. Find the two downloads here: Ring Support Ring Infill 3. Import the STLs into Repetier Host and assign each STL to an extruder using the Extruder drop down menu.
  • Page 25 5. Select desired Slic3r settings as before and click “Slice with Slic3r”. 6. Save the G-Code created. NOTE: You must always calibrate the first extruder and then the second one. If you only plan to use one, you can calibrate the other at any position. However, you must still press ‘Calibrate.’...
  • Page 26 Printing Two STL Designs 1. Load your two separate syringes with Pluronic and attach 30 gauge 0.25” needles (pink). 2. Connect your Allevi 2 to your computer. 3. Home Z. Home E. Home X/Y. 4. Select “Petri Dish” from the “Select Plate Type” menu. Then click “Select well plate”.
  • Page 27 b. Adjust pressure between approximately 30-50 PSI for pluronic until material extrudes continuously. 6. Use the same step controls from before to bring the bed plate to point where the dish is almost touching the needle. 7. Once the Z axis is in place, press “extrude” for Extruder 1 to turn the pressure on and deposit a small amount of material, then quickly press “extrude”...
  • Page 28 Next, use X/Y controls as well to line up the right extruder directly on top of the dot you created with the left extruder. 10. Once you have the correct orientation, click “Update” under Extruder 2, then “Calibrate.” 11. Load your dual print G-Code file.
  • Page 29 12. Click “Load and Print” on the dual gcode file. 13. Pat yourself on the back! You’re on your way to becoming a 3D bioprinting master!
  • Page 30 BIOPRINTING YOUR APPLICATION Now, to the point. You wanted an Allevi bioprinter so that you can print what you care about. We’ve carefully designed protocols to prepare you for any application that you want to bioprint. These are challenges that are based on replicating landmark papers published in the field of bioprinting. We want to show you that bioprinting with Allevi gets you further, faster.
  • Page 31 Resetting BioPrint Password On MAC 1. Open Finder and select "Go to Folder" from the "Go" menu in the menubar 2. Enter `/Users/[your computer username]/Library/Application Support` as the folder to go to. If your computer username is Allevi you will enter `/Users/Allevi/Library/Application Support` here.
  • Page 32 Resetting BioPrint Password on Windows 1. Navigate to “C:\Users\<username>\” 2. Unhide hidden items to view the “AppData” folder a. As depicted below, navigate to “View” and check the box next to “Hidden Items” 3. Navigate into “…\AppData\Roaming\” 4. Delete the BioPrint Folder Connection Issues on Mac If you are having trouble with reconnecting your printer on a MAC.
  • Page 33 TROUBLESHOOTING AND MORE INFORMATION When you buy an Allevi bioprinter, you’re not just getting a biofabrication platform; you’re gaining the Allevi Advantage, which is all the experience and expertise Allevi has to offer. One very useful resource is Allevi’s Build With Life repository.
  • Page 34 Allevi, Inc | 3401 Grays Ferry Ave | Building 176 | Philadelphia PA | 19146 Copyright Allevi, Inc 2018...