MAKING A STANDARD % BRIX SOLUTION ....... 9 TO CHANGE TEMPERATURE UNIT ..........9 BATTERY REPLACEMENT ............10 WARRANTY................10 ERROR MESSAGES ..............11 PRELIMINARY EXAMINATION Each HI 96811 instrument is supplied with: • 9 V battery • Instruction manual...
The HI 96811 is optical instruments that are based on the measurement of the refractive index of a solution. The measurement of refractive index is simple and quick and provides the vintner a standard accepted method for sugar content analysis.
In the HI 96811, light from an LED passes through a prism in contact with the sample. An image sensor determines the critical angle at which the light is no longer refracted through the sample.
The HI 96811 converts the refractive index of the sample to sucrose concentration in units of percent by weight, %Brix (also referred to as °Brix). The conversion used is based on the ICUMSA Methods Book (International Commission for Uniform Methods of Sugar Analysis). Since the majority of sugar in grape juice is fructose and glucose and not sucrose, the reading is sometimes referred to as “Apparent Brix”.
DISPLAY ELEMENTS 1. Battery (blinks when low battery condition detected) 2. Primary Display (displays measurement and error messages) 3. Measurement in Progress Tag 4. SETUP: Factory Calibration Tag 5. CAL: Calibration Tag 6. Measurement Unit 7. Automatic Temperature Compensation (blinks when temperature exceeds 10-40 °C / 50-104 °F range) 8.
CALIBRATION PROCEDURE Calibration should be performed daily, before measurements are made, when the battery has been replaced, between a long series of measurements, or if environmental changes have occurred since the last calibration. 1. Press the ON/OFF key, then release. Two instrument test screens will be displayed briefly;...
MEASUREMENT PROCEDURE Verify the instrument has been calibrated before taking measurements. 1. Wipe off prism surface located at the bottom of the sample well. Make sure the prism and sample well are completely dry. 2. Using a plastic pipette, drip sample onto the prism surface. Fill the well completely.
MAKING A STANDARD % BRIX SOLUTION To make a Brix Solution, follow the procedure below: • Place container (such as a glass vial or dropper bottle that has a cover) on an analytical balance. • Tare the balance. • To make an X BRIX solution weigh out X grams of high purity Sucrose (CAS #: 57-50-1) directly into the container.
BATTERY REPLACEMENT To replace the instrument’s battery, follow these steps: • Make sure the instrument is off. • Turn instrument upside down and remove the battery cover by turning it counterclockwise. • Extract the battery from its location. • Replace with new 9V battery making certain to observe polarity. •...