Invitrogen Qubit 2.0 User Manual
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Invitrogen Qubit 2.0 User Manual

Fluorometer
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®
Qubit
2.0 Fluorometer
Catalog no. Q32866
Rev. Date: 4 October 2010
MAN0003231
User Manual

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Summary of Contents for Invitrogen Qubit 2.0

  • Page 1 ® Qubit 2.0 Fluorometer Catalog no. Q32866 Rev. Date: 4 October 2010 MAN0003231 User Manual...

  • Page 2: Table Of Contents

    Contents Product Contents......................3 Safety Information ......................4 Introduction..................5 ® Description of Qubit 2.0 Fluorometer ..............5 Product Specifications ....................8 Methods ....................9 Getting Started.......................9 ® Guidelines for Using the Qubit 2.0 Fluorometer ..........13 ® Calibrating the Qubit 2.0 Fluorometer..............14 Reading Samples ......................19 Calculating the Original Sample Concentration ............22 Cleaning and Maintenance..................29 Troubleshooting ......................30...

  • Page 3: Product Contents

    See page 9 to install the instrument. Visit probes.invitrogen.com/qubit to register your instrument. Registering Your You will be asked to supply the serial number, your name, and Instrument your contact details.

  • Page 4: Safety Information

    Safety Information Review and follow the safety instructions below. Safety Precautions  Do not install the instrument in heavy humidity, such as in a greenhouse or an incubator, to avoid a danger of electric shock. If water or other material enters the instrument, the adaptor, or power inlet, disconnect the power cord and contact a service person.

  • Page 5: Introduction

    Introduction ® Description of Qubit 2.0 Fluorometer ® ® The Qubit 2.0 Fluorometer is a benchtop fluorometer for the Qubit quantitation of DNA, RNA, and protein, using the highly sensitive Fluorometer ™ and accurate fluorescence-based Qubit quantitation assays. Use of the state-of-the-art dyes selective for dsDNA, RNA, and protein minimizes the effects of contaminants in your sample that affect the quantitation.
  • Page 6 ® Description of Qubit 2.0 Fluorometer, Continued The image below shows the location of the various parts of the Instrument ® Qubit 2.0 Fluorometer. Exterior Components ® Power Inlet connects the Qubit 2.0 Fluorometer to an electrical outlet using the supplied power cord and the appropriate plug, based on the electrical outlet configuration in your country.
  • Page 7 ® Description of Qubit 2.0 Fluorometer, Continued ® Use the touch screen of the Qubit 2.0 Fluorometer to operate the User Interface instrument. The touch screen consists of:  The touch screen buttons to operate the instrument, including alphanumeric buttons to enter sample names. ...

  • Page 8: Product Specifications

    Product Specifications ® Environmental Conditions Qubit Fluorometer Operating power: 100–240 VAC, 0.4 A Specifications Frequency: 50–60 Hz Electrical output: 9 VDC, 1.33 A Installation site: Indoor use only Operating temperature: 15–40°C Maximum relative humidity: 20–80%, non-condensing Instrument Specifications Instrument type: Benchtop fluorometer Instrument dimensions: 5.3”...

  • Page 9: Methods

    Methods Getting Started ® The Qubit 2.0 Fluorometer is a stand-alone instrument that does Installing the ® not require a connection to a computer. Qubit Fluorometer After unpacking the instrument, place the instrument on a flat, level, dry surface. ® Plug one end of the supplied power cord into the Qubit Fluorometer.
  • Page 10 Getting Started, Continued ® The Qubit 2.0 Fluorometer has an auto-standby (i.e., sleep) mode Sleep Mode that is triggered after 30 minutes of inactivity. You can also trigger the stand-by mode by touching the Power button ( ) located on the top left of the touch screen.
  • Page 11 Getting Started, Continued The date and time is already preset when you receive the Date and Time instrument. To reset the date and time, follow these steps: Setup Press Date/Time. The Date/Time Properties Screen contains 6 roller wheels that respond to movement of a finger across the screen as if they were wheels.
  • Page 12 ® To update your Qubit 2.0 Fluorometer with the latest firmware Updating the available from Invitrogen, follow these steps: Firmware Download the latest firmware to your USB drive from our website (www.invitrogen.com/qubit). Press the Version button located on the top portion of the touch screen.

  • Page 13: Guidelines For Using The Qubit

    30 seconds before taking another reading.  Visit www.invitrogen.com/qubit for additional application notes, technical notes, citations, firmware updates, and a list ™ of validated Qubit assays that have been tested using the ®...

  • Page 14: Calibrating The Qubit ® 2.0 Fluorometer

    ® Calibrating the Qubit 2.0 Fluorometer For each assay, you have the choice to run new standards for Introduction ® calibrating the Qubit 2.0 Fluorometer or to use the values from the previous calibration (for more information, see Calibrating the ®...
  • Page 15 ® Calibrating the Qubit 2.0 Fluorometer, Continued If you have already performed a calibration for the selected Running New ® assay, Qubit 2.0 Fluorometer will prompt you to choose Standards for between reading new standards and using the previous Calibration, calibration.
  • Page 16 ® Calibrating the Qubit 2.0 Fluorometer, Continued Insert Standard #1 into the Sample Chamber and press Read. Running New Ensure that you are using the Standard #1 appropriate for the Standards for assay you are performing. The reading takes approximately Calibration, 3 seconds.
  • Page 17 ® Calibrating the Qubit 2.0 Fluorometer, Continued  In the Fluorescence vs. Concentration graph, open circles represent correct standards, black circles represent samples that fall within the assay's range, and red circles represent samples or standards that are not within the assay’s range. The most recent sample is signified by a large black circle.
  • Page 18 ® Calibrating the Qubit 2.0 Fluorometer, Continued The Check Standards Screen displays the raw fluorescence values Checking for Standard #1, Standard #2, Standard #3 (if applicable), and the Standards last measurement taken for a given assay. These values can assist you in making a judgment regarding the performance of the ®...

  • Page 19: Reading Samples

    Reading Samples ® After you have completed the calibration of the Qubit Introduction Fluorometer by running the appropriate standards for the assay you want to use or have accepted the values from the previous calibration, you are ready to read your samples. This section ®...
  • Page 20 Reading Samples, Continued Insert a sample into the Sample Chamber and press Read. The Reading measurement takes approximately 3 seconds. Samples, continued Upon the completion of the measurement, the result is displayed on the screen. The number displayed is the concentration of the nucleic acid or protein in the assay tube.
  • Page 21 Reading Samples, Continued  Notes on Using Pressing Hide Graph hides the Fluorescence vs. Concentration graph and the screen only displays the latest measurement. Graphs Note that the Hide Graph function is not available on the Standards Screen.  Pressing Show Graph brings the graph back onto the screen. ...

  • Page 22: Calculating The Original Sample Concentration

    Calculating the Original Sample Concentration ® The Dilution Calculator feature of the Qubit 2.0 Fluorometer Introduction calculates the concentration of your original sample based on the volume of sample you have added to the assay tube. To calculate the concentration of your original sample, press Dilution Calculate Stock Conc.
  • Page 23 Calculating the Original Sample Concentration, Continued To change the units in which the original sample Dilution concentration is displayed, press ng/mL. A pop-up window Calculator, showing the current unit selection (as indicated by an adjacent continued red dash) opens. Select the unit for your original sample concentration by touching the desired unit in the unit selection pop-up window.
  • Page 24 Calculating the Original Sample Concentration, Continued ® To save the data from your calculation to the Qubit Saving the Fluorometer, press Save on the Dilution Calculator Screen. Calculation to ® the Qubit Fluorometer The last calculated value of your measurement is saved in the .CSV file and tagged with a time and date stamp.
  • Page 25 Data Handling ® The Qubit 2.0 Fluorometer presents comprehensive data with Introduction graphic reports, and it allows saving of data as a .CSV (comma separated value) file for sample comparisons. Each measurement data point in the .CSV file is numbered and exhibits a time and date stamp.
  • Page 26 Data Handling, Continued Select the data file to rename by touching the corresponding Renaming Data line of the spreadsheet on the Data Screen. The selected line Files will be highlighted. If no lines are selected, you are prompted to select a line or multiple lines to rename. Note: To select multiple files, highlight each line by-one-one or press the column headers to highlight all files.
  • Page 27 Data Handling, Continued ® The Qubit 2.0 Fluorometer is designed for stand-alone use; it does Saving the not require the use of an external computer. However, to archive Data to the ® data and generate reports, you may transfer the numeric data Qubit 2.0 USB stored in the .CSV file to your computer using the USB drive and...
  • Page 28 Data Handling, Continued Select the data file to save by touching the corresponding line Saving the of the spreadsheet on the Data Screen. The selected line is Data to the ® highlighted. Qubit 2.0 USB Note: By default, the entire .CSV file will be saved to the USB drive if Drive, continued no lines are selected...

  • Page 29: Cleaning And Maintenance

    Cleaning and Maintenance ® The Qubit 2.0 Fluorometer does not need regular maintenance. To Cleaning and troubleshoot problems with the instrument, contact Technical Maintenance Support (page 38). Do not perform any repairs or service on the ® Qubit 2.0 Fluorometer to avoid damaging the instrument. ...

  • Page 30: Troubleshooting

    Troubleshooting  Handling ™ The calibration standards included in the Qubit RNA and Samples ™ Qubit RNA BR Assay Kits are high-quality rRNA standards. The integrity and concentration of these standards is critical to ™ the optimal performance of the Qubit RNA assay.
  • Page 31 Troubleshooting, Continued Low Reading  Your sample is out of range. Use a sample that is more concentrated or use a lower dilution (i.e., 20 μL in 180 μL instead of 10 μL in 190 μL).  View the Check Standards Screen to confirm that the values for the samples fall between the values of the standards (see page 18).
  • Page 32 Troubleshooting, Continued High Reading  Your sample is out of range. Use a sample that is less concentrated.  View the Check Standards Screen to confirm that the values for the samples fall between the values of the standards (see page 18).

  • Page 33: Appendix

    Appendix ™ Critical Qubit Assay Considerations ® The Qubit 2.0 Fluorometer generates concentration data based on How the ® the relationship between the two Standards used in calibration Qubit ™ (three for the Qubit protein assay). The plot below shows the line Fluorometer corresponding to the curve-fitting algorithm (a modified Hill plot) Calculates...
  • Page 34 ™ Critical Qubit Assay Considerations, Continued ™ The Qubit assays were designed to be performed at room Assay temperature (22–28°C), and temperature fluctuations can influence Temperature the accuracy of the assay. To minimize temperature fluctuations, store all kit reagents at room temperature and insert all assay tubes ®...
  • Page 35 ™ Critical Qubit Assay Considerations, Continued ™ ® The Qubit reagents exhibit high photostability in the Qubit Photobleaching ™ Fluorometer, showing <0.3% drop in fluorescence after 9 readings of Qubit and <2.5% drop in fluorescence after 40 readings. It is important Reagents to remember, however, that if the assay tube remains in the ®...

  • Page 36: Accessory Products

    Accessory Products ® ® The following products can be used with the Qubit 2.0 Fluorometer Qubit and are available separately from Invitrogen. Products For more information, visit www.invitrogen.com or contact Technical Support (page 38). Product Quantity Catalog no. ® Qubit 2.0 Fluorometer...

  • Page 37: Regulatory Requirements

    Regulatory Requirements ® The Qubit 2.0 Fluorometer has been tested and complies with the following regulatory requirements: U.S. and Canadian Safety Standards UL 61010-1:2004 for U.S. CAN/CSA-C22.2 No.61010-1:2004 for Canada U.S. and Canadian EMC Requirements FCC CFR 47, Part 15 Sections 15.107 and 15.109 (Class B) Industry Canada Interference Causing Equipment Standard ICES-003 Issue 4, Sections 5.3 and 5.5.

  • Page 38: Technical Support

    Technical Support Visit the Invitrogen website at www.invitrogen.com for: Web Resources  Technical resources, including manuals, vector maps and sequences, application notes, MSDSs, FAQs, formulations, citations, handbooks, etc.  Complete technical support contact information  Access to the Invitrogen Online Catalog ...

  • Page 39: Purchaser Notification

    Purchaser Notification The purchase of this product conveys to the purchaser the limited, Limited Use non-transferable right to use the purchased amount of the product Label License only to perform internal research for the sole benefit of the purchaser. No. 358: No right to resell this product or any of its components is conveyed Research Use expressly, by implication, or by estoppel.
  • Page 40 Warranty will be free from defects in materials and workmanship for a period of one (1) year from the date of delivery. Invitrogen agrees, as its sole responsibility under this limited warranty, and upon prompt notice of a defect, to repair, replace or credit the purchase price, at its discretion, any Instrument discovered to be defective within the warranty period.
  • Page 41 Corporate Headquarters 5791 Van Allen Way Carlsbad, CA 92008 T: 1 760 603 7200 F: 1 760 602 6500 E: tech_support@invitrogen.com For country-specific contact information visit our web site at www.invitrogen.com...

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