Contents 1.0 Introduction Lucetta™ 2 Luminometer Overview Light Detection 2.0 General Use Instructions Use Restrictions Maintenance Safety Instructions and Use Precautions Storage Conditions Batteries Return Shipment Instrument Disposal 3.0 Setup Instructions Lucetta™ 2 Luminometer Components Setup Instructions Optimal Operation Temperature Battery Operation Electrical Outlet Operation Consumables...
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4.0 Lucetta™ 2 Luminometer Operation Menu MycoAlert™ PLUS/ MycoAlert™ Run Single Read Run 5.0 Data Storage and PC Upload Maximum Storage Capacity Viewing Logged Measurements Upload to a PC 6.0 Setting Instrument Parameters Password Customer Settings Date & Time System Information 7.0 Troubleshooting 8.0 Error Codes...
– Single Read mode: allows the user to obtain unprocessed The Lucetta™ 2 Luminometer is low maintenance. It should be protected luminescence readings. This mode can be used for Lonza’s from dust and debris. ViaLight™ or ToxiLight™ Assay or other luminescent assays.
Do not operate the instrument if internal components – Read and understand the the Lucetta™ 2 Luminometer Manual have been exposed to fluids. The instrument must be cleaned by Lonza prior to using the instrument. All potential users should have authorized personnel.
2.6 Return Shipment If the instrument has to be returned to Lonza for servicing or inspection, please follow the instructions below: – Contact the Lonza Scientific Support team to obtain the required decontamination and repair form. Complete both forms before return shipment.
3.3 Optimal Operation Temperature It is strongly recommended to operate luminescence measurements at room temperature (20°C) to achieve the performance characteristics listed in the technical data. The general operating range is 15–30°C and below 70% humidity. 3.4 Battery Operation The Lucetta™ 2 Luminometer is equipped with a rechargeable battery. –...
End user settings can be protected with a password here. The submenu the display. “MycoAlert Settings” is only accessible by Lonza personnel. 11. Press OK to continue with the next sample. 12. After reading B is finished for all samples, press OK. The complete results for all samples will be displayed (starting with 4.2 MycoAlert™...
Interpretation of Results 1. Turn on the instrument by pressing the ON button. 2. Use the arrow button to select RUN SINGLE READ and press 1. MycoAlert™ PLUS Assay OK. Navigate up to ASSAY PROTOCOL NAME and press OK. Use arrow buttons ...
5.0 Data Storage and PC Upload The Lucetta™ 2 Luminometer has excellent data storage and Display data transfer capabilities. Each completed measurement is logged in the starting at: Date: 10/18/2018 instrument’s internal memory. When the maximum storage capacity is Time: 09:40 reached, the oldest data are automatically overwritten.
Instrument settings for MycoAlert measurements are password- 5. Create Protocol name. Name is limited to 10 characters. protected and can only be accessed by Lonza service personnel. 6. Use arrow button and select LIMIT FAIL number. Software version and serial number of the luminometer can also be 7.
6.4 System Display brightness and contrast are adaptable to the lighting conditions. Assay: Protocol 1. Navigate down to SETTINGS and press OK. Limit Fail: 10000 2. Select SYSTEM and press OK. Limit Pass: 5000 3. To adjust brightness, press OK. Use arrow buttons to Meas.
8.0 Error Codes Error Message Action Display - 10 Turn off instrument and allow it to dry fully. Switch instrument on. If error message appears again, contact Lonza Scientific Support. Other error messages Contact Lonza Scientific Support 9.0 Appendix 9.1 Factory Settings for Single Read...
9.2 Technical Data Sample and Data Handling Sample Tube Format Transparent polystyrol tubes and microcentrifuge tubes Diameter: maximum up to 15 mm; minimum approximately 12 mm Length: 47-75 mm Detector Ultra fast single photon counter with spectral sensitivity range of 380-630 nm Sensitivity 1 fmol ATP using the ATP Bioluminescence Assay Kit CLSII (Boehringer Mannheim, FRG) Background Value...
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