1. Manuals
  2. Brands
  3. Life technologies Manuals
  4. Laboratory Equipment
  5. Invitrogen Tali
  6. User manual

Life technologies Invitrogen Tali User Manual

Image-based cytometer
Hide thumbs
Table of Contents

Advertisement

Quick Links

Download this manual
user guide
Tali
Image-Based Cytometer
™
Catalog Number T10796
Publication Part Number MAN0003766
Revision Date 28 April 2011

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the Invitrogen Tali and is the answer not in the manual?

Questions and answers

Related Manuals for Life technologies Invitrogen Tali

Summary of Contents for Life technologies Invitrogen Tali

  • Page 1 user guide Tali Image-Based Cytometer ™ Catalog Number T10796 Publication Part Number MAN0003766 Revision Date 28 April 2011...

  • Page 2: Table Of Contents

    Contents Product Contents............................3 Introduction ........................4 Description of Tali ™ Image-Based Cytometer..................4 Specifications............................9 Methods ........................11 Getting Started............................11 Guidelines for Performing Tali ™ Assays....................14 Select a Tali ™ Assay ..........................15 Load Sample ............................16 Focus Image............................18 Measure the Background (optional).....................19 Run Sample............................20 Analyze Data ............................25 Export Data ............................28 Maintenance .......................

  • Page 3: Product Contents

    Product Contents ™ The Tali Image-Based Cytometer (Cat. no. T10796) is shipped with the Tali Image-Based ™ components listed below: Cytometer Component Quantity ™ Tali Image-Based Cytometer 1 each ™ Tali Cellular Analysis Slides, box of 50 1 box ™ Tali Image-Based Cytometer power cords, pack of 4 cords 1 each...

  • Page 4: Introduction

    Introduction Description of Tali Image-Based Cytometer ™ ™ The Tali Image-Based Cytometer is a 3-channel (bright field, green fluorescence, Tali ™ Image-Based red fluorescence) benchtop assay platform that uses state-of-the-art optics and Cytometer image analysis to perform assays for cells in suspension, including GFP and RFP expression, apoptosis, cell viability (live, dead, and total cells), and cell counting assays.
  • Page 5 Description of Tali ™ Image-Based Cytometer, continued ™ The Tali Image-Based Cytometer simultaneously captures a series of bright field How Tali Image- ™ ™ and fluorescent images (i.e., fields of view) of the sample in the Tali Cellular Based Cytometer Analysis Slide and uses sophisticated digital image analysis algorithms to determine counts cells ™...
  • Page 6 Description of Tali ™ Image-Based Cytometer, continued ™ The front view of the Tali Image-Based Cytometer illustrating the various parts of Front view of Tali ™ the instrument is shown below. Image-Based Power Button Cytometer The power button is used to turn the instrument on and off. The blue status light indicates that the instrument is on.
  • Page 7 Description of Tali ™ Image-Based Cytometer, continued ™ The rear and side view of the Tali Image-Based Cytometer illustrating the various Rear and side view parts of the instrument is shown below. of Tali ™ Image- Power Inlet Based Cytometer The power inlet connects the instrument to an electrical outlet through the supplied power cord and the appropriate plug, based on the electrical outlet configuration in your country.
  • Page 8 Description of Tali ™ Image-Based Cytometer, continued ™ The touch screen user interface of the Tali Image-Based Cytometer is used to User interface operate the instrument and consists of:  The touch screen buttons to operate the instrument  The digital display to show the image of cells and data from the sample The example below shows the Measure screen of the Apoptosis assay.

  • Page 9: Specifications

    Specifications Instrument type: Benchtop cell counter and suspension cell-based Physical assay platform characteristics 11 ½” (W)  11 ½” (H)  17 ½” (D) Instrument dimensions: Weight: 19.4 lbs Operating power: 100–240 VAC, 2.5 A, 120 V Frequency: 50/60 Hz Electrical input: 12 VDC, 13 A Installation site:...
  • Page 10 Using the Tali ™ Image-Based Cytometer ™ The Tali Image-Based Cytometer captures a series of images (i.e., fields of view) of Workflow ™ the sample in Tali Cellular Analysis Slides and uses sophisticated digital image ™ analysis algorithms to perform assays for cells in suspension. All Tali Assays share the same basic workflow and user interface, but differ in which channels the images are captured and which algorithm is used for analysis.

  • Page 11: Methods

    Methods Getting Started ™ This section provides information on installing your Tali Image-Based Cytometer, Introduction setting the date and time, and updating the firmware. It also provides information on adjusting the counting settings to optimize the analysis of different sample types. 1.
  • Page 12 Getting Started, continued Press Settings on the navigation bar to display the Settings screen. Settings screen The Settings screen allows you to:  Calibrate and align the instrument to ensure optimal performance (page 32)  Update to install new firmware versions as they become available (page 31) ...
  • Page 13 Getting Started, continued ™ The Tali Image-Based Cytometer comes with pre-set parameters to match the Adjust counting majority of cultured cell types which can be accurately counted without changing settings ™ any of the default parameters. Counting settings of the Tali Image-Based Cytometer allows you to adjust the global image analysis parameters for specific or mixed cell types;...

  • Page 14: Guidelines For Performing Tali Assays

    Guidelines for Performing Tali ™ Assays To obtain the best results, follow these recommendations: General guidelines  Wear gloves during sample handling.  ™ Do not touch the optical surfaces of the Tali Cellular Analysis Slides. Hold the ™ slides by the edges. See page 16 for guidelines on loading the Tali Cellular Analysis Slides.

  • Page 15: Select A Tali Assay

    Select a Tali ™ Assay ™ 1. To select a Tali Assay, press the appropriate assay button (Quick count, Select a Tali Assay ™ Viability, Apoptosis, or GFP/RFP) on the Home screen. In this example, the Apoptosis Assay is selected. ™...

  • Page 16: Load Sample

    Load Sample ™ The Tali Cellular Analysis Slides are plastic, disposable slides that hold the sample Load the Tali ™ in two separate, enclosed chambers (A and B) that allow the analysis two different Cellular Analysis samples or replicates of the same sample. Each chamber has a 25-μL sample Slide ™...

  • Page 17: Load Your Sample

    Load Sample, continued ™ 1. Load 25 μL of your sample (per slide chamber) into the Tali Cellular Analysis Insert the Tali ™ Slide as described on page 16. Cellular Analysis ™ 2. The Tali Image-Based Cytometer counts the cells in the chamber opposite the Slide into the loading direction.

  • Page 18: Focus Image

    Focus Image 1. Before running your sample, focus your cells using the image adjustment Focus the field of (focus) knob on the right side of the instrument. view 2. Touch Zoom to review the image.  Correctly focused images have uniformly dark-colored cells surrounded by bright halos.

  • Page 19: Measure The Background (Optional)

    Measure the Background (optional) Measuring the background fluorescence using unstained samples allows you to Measure the obtain more accurate sample counts. Note that the Background feature is not background available for the Quick count assay, because this assay does not use fluorescent (optional) ™...

  • Page 20: Run Sample

    Run Sample 1. To specify the number of fields of Capture images view to capture, touch the arrow button on the # of images to capture drop-down menu and select from the available options. 2. To capture the specified number of fields of view, touch Press to run sample. While the image capture is in progress, an ongoing update is provided by the progress bar.

  • Page 21: Review Captured Images

    Run Sample, continued 1. To select a captured field of view for review, press the thumbnail of the image Review captured in the Zoom tab. images 2. To zoom into and out of the selected image, use the zoom slider bar by touching the red dot and moving it horizontally.
  • Page 22 Run Sample, continued 1. To review images captured through different channels (bright field, green Reviewing layers fluorescence, red fluorescence), select the image by touching the thumbnail in the Zoom tab, and then press the Layers tab. Note: The number of layers that are available for viewing and the layer button labels depend on the Tali ™...

  • Page 23: Set Gates And Thresholds

    Run Sample, continued ™ The Tali Image-Based Cytometer allows you to set gates or thresholds on cell size Set gates and and relative fluorescence from available channels for data collection. After capturing thresholds images of your sample, follow the instructions below to set the thresholds for cell ™...
  • Page 24 Run Sample, continued 5. To set the threshold for Annexin V fluorescence, touch the Annexin V Set gates and thumbnail histogram. thresholds, The pop-up window displays the “# of cells vs. Fluorescence” histogram for continued Annexin V fluorescence, the slider bar for adjusting the threshold for fluorescence, and the buttons for displaying the Sample and/or the Control fluorescence data in the graph.

  • Page 25: Analyze Data

    Analyze Data ™ The Tali Image-Based Cytometer working memory holds 145 Gigabytes of data, Introduction sufficient for storing numeric and graphic data files from 1000 sample runs. You can access the stored data files through the Data tab, where you can analyze, annotate, rename, or delete them.
  • Page 26 Analyze Data, continued 1. Select the data file to rename by touching the corresponding line of the file list. Rename data files The selected line will be highlighted. 2. To rename the selected file(s), touch Rename. The alpha-numeric keyboard screen is displayed. 3.
  • Page 27 Analyze Data, continued ™ 1. To review the data from counts previously saved in the Tali Image-Based Review stored data Cytometer, select the desired file from the Data screen. 2. Press Layers, Zoom, or Analysis tabs to display the corresponding screens. 1.

  • Page 28: Export Data

    Export Data ™ The Tali Image-Based Cytometer is designed for stand-alone use; it does not require Data export options the use of an external computer. However, to archive data and generate reports, you may transfer the data stored in the instrument to your computer using the USB drive. ™...
  • Page 29 Export Data, continued ™ 1. Insert the Tali Image-Based Cytometer USB Drive (or any other USB drive) Export data ™ into one of the USB ports on the Tali Image-Based Cytometer. 2. Select the data file to export by touching the corresponding line of the file list. The selected line will be highlighted.
  • Page 30 Export Data, continued The image below shows an example of a cell analysis report exported as a .pdf file. Cell analysis report...

  • Page 31: Maintenance

    Maintenance Update the Tali ™ Image-Based Cytometer Firmware ™ The update feature allows you to update the Tali Image-Based Cytometer when Introduction new firmware versions are available. This ensures the optimal performance of the instrument and adds new assays and features, if available. ™...

  • Page 32: Adjust The Camera Alignment

    Adjust the Camera Alignment ™ The proper alignment of the two cameras inside the Tali Image-Based Cytometer Introduction is essential for obtaining the most accurate data from the instrument. Follow the protocol below to align the cameras so that the fluorescent image is properly ™...
  • Page 33 Adjust the Camera Alignment, continued 8. Once the alignment has been processed, use the directional keys to position the Align the cameras, ™ green circles over the corresponding Tali Alignment Bead. continued 9. Touch Set alignment to confirm the correct alignment of the cameras. To go back to the previous screen without completing the alignment, touch Cancel.

  • Page 34: Calibrate The Tali Image-Based Cytometer

    Calibrate the Tali ™ Image-Based Cytometer ™ Calibrating the green and red fluorescent channels of the Tali Image-Based Introduction Cytometer sets the dynamic range of the instrument. The calibration is most effective when performed after the alignment sequence. ™ Tali Calibration Beads (Cat.
  • Page 35 Calibrate the Tali™ Image-Based Cytometer, continued 5. When the green channel calibration is complete, the cellular analysis slide will Calibrate the green automatically be ejected and the Red channel calibration dialog box will be and red channels, displayed. continued ™ ™...

  • Page 36: Clean The Tali Image-Based Cytometer

    Clean the Tali ™ Image-Based Cytometer ™ We recommend that the Tali Image-Based Cytometer be cleaned periodically to Introduction prevent the buildup of dust and dirt that might reduce its performance and cause contamination.  ™ To avoid electrical shock, always turn off the Tali Image-Based Cytometer and Important unplug the power cord before cleaning or decontaminating the instrument.

  • Page 37: Appendix

    Appendix Safety Information Review and follow the safety instructions below. Safety precautions  Do not install the instrument in heavy humidity such as a greenhouse or an incubator to avoid a danger of electric shock. If water or other material enters the instrument, the adaptor, or power inlet, disconnect the power cord and contact a service person.
  • Page 38 Safety Information, continued ™ The symbols used on the Tali Image-Based Cytometer are explained below. Symbols Used on the instrument to indicate a warning (caution, risk of danger). Consult the manual to avoid possible personal injury or instrument damage. Protective conductor terminal (main ground) WEEE (Waste Electrical and Electronic Equipment) symbol indicates that this product should not be disposed of in unsorted municipal waste.

  • Page 39: Accessory Products

    Accessory Products ™ Some of the components of Tali Image-Based Cytometer are also available Tali Image-Based ™ separately from Invitrogen. These products are listed below. For more Cytometer information, refer to www.invitrogen.com or contact Technical Support products and (see page 40). accessories Product Amount...

  • Page 40: Technical Support

    Technical Support Visit the Invitrogen website at www.invitrogen.com for: Web resources  Technical resources, including manuals, vector maps and sequences, application notes, FAQs, formulations, citations, handbooks, etc.  Complete technical support contact information  Access to the Invitrogen Online Catalog ...

  • Page 41: Purchaser Notification

    Purchaser Notification Invitrogen (a part of Life Technologies Corporation) is committed to providing our Limited Warranty customers with high-quality goods and services. Our goal is to ensure that every customer is 100% satisfied with our products and our service. If you should have any questions or concerns about an Invitrogen product or service, contact our Technical Support Representatives.
  • Page 42 Headquarters 5791 Van Allen Way | Carlsbad, CA 92008 USA | Phone +1 760 603 7200 | Toll Free in USA 800 955 6288 For support visit www.invitrogen.com/support or email techsupport@invitrogen.com www.lifetechnologies.com...

Table of Contents