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Chemyx Fusion Series User Manual

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CHEMYX
Fusion Series User Manual
Chemyx Inc.
10905 Cash Road
Stafford, TX 77477 USA
www.chemyx.com
Updated: 2016-11-08

Intellectual Property

All Intellectual Properties, as defined below, owned by or which is otherwise the property of
Chemyx Inc. or its suppliers relating to the Chemyx syringe pumps, including but not limited to,
accessories, parts or software relating thereto (Chemyx Syringe Pumps), are proprietary to
federal and state laws, and international treaty provisions. Intellectual Property includes but is
not limited to, inventions (patentable or unpatentable), patents, trade secrets, copyrights,
software, firmware, computer programs, and related documentation and other works of
authorship. Moreover, you agree that you will not, and will not attempt to, modify, prepare
derivative works of, reverse engineer, disassemble the Chemyx syringe pumps, decompile or
otherwise attempt to create source code from the related software/firmware. No title to or
ownership in Intellectual Property is transferred to you. All applicable rights of the Intellectual
Property shall remain with Chemyx and its suppliers.

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  • Page 1: Intellectual Property

    All Intellectual Properties, as defined below, owned by or which is otherwise the property of Chemyx Inc. or its suppliers relating to the Chemyx syringe pumps, including but not limited to, accessories, parts or software relating thereto (Chemyx Syringe Pumps), are proprietary to federal and state laws, and international treaty provisions.

  • Page 2: Eu Declaration Of Conformity (Doc)

    Fusion Series User Manual Chemyx, Inc. EU Declaration of Conformity (DoC) The PDF version of the latest DoC can be downloaded at www.chemyx.com/support.

  • Page 3: Table Of Contents

    Fusion Series User Manual Chemyx, Inc. INTELLECTUAL PROPERTY EU DECLARATION OF CONFORMITY (DOC) MANUAL DESCRIPTION GENERAL INFORMATION AFETY NFORMATION ARRANTY AND EPAIR NFORMATION IMITED ARRANTY EPAIR ACILITIES AND ARTS IXEL OLICY ERIAL UMBERS ALIBRATIONS PRODUCT OVERVIEW USION ERIES YRINGE UMPS...
  • Page 4 Fusion Series User Manual Chemyx, Inc. NTERING ALUES NTERING UMBERS NTERING ETTERS ELECTION CREEN ASIC YRINGE ELECTION ARGET RANSFER OLUME NFUSION ITHDRAWAL USION ONLY TART ELAY PTIONS Flow-Rate Units Saving Run Parameters Priming/Bolus Rate Enable Volume Lock ULTI ETUP Syringe Selection...
  • Page 5 Fusion Series User Manual Chemyx, Inc. PUMP CONTROL BY COMPUTER ABLE EQUIREMENTS ONNECTING ETTINGS OMMANDS HEMYX ONTROLLER ROGRAMS PUMP MAINTENANCE UBRICATING THE EPLACING THE PUMP ACCESSORIES WITCH ONNECTING THE WITCH SING THE WITCH 10-C HANNEL YRINGE USION ONLY TTACHING THE...

  • Page 6: Manual Description

    Please read the following safety precautions to ensure personal safety and operational longevity of the Chemyx syringe pump. Chemyx is not responsible for the equipment if used in a manner not specified by the manufacturer; warranty coverage provided by the equipment may be dropped as a result.

  • Page 7: Warranty And Repair Information

    Warranty and Repair Information Limited Warranty Chemyx provides a two-year warranty from the shipment date for its pumps against defects in materials and workmanship. Chemyx will repair any product that proves defective during its stated warranty period. The foregoing warranty will not apply to damage resulting from: ●...

  • Page 8: Repair Facilities And Parts

    Although the pump is highly accurate, syringes of different materials can attribute significant volume errors. Typically, these errors can range from 1% for glass syringes to 5% for plastic syringes. Chemyx is not responsible for errors generated from syringes.

  • Page 9: Product Overview

    A syringe pump is a small, positive-displacement pump used to gradually transfer precise volumes of fluid. All Chemyx Fusion-series syringe pumps are driven via a stepper motor. This stepper motor precisely turns a lead screw that is threaded through a pusher block, which causes the pusher block to move.
  • Page 10 When the stepper motor turns in the opposite direction, the pusher block moves such that the syringe plunger is pulled, thus drawing fluid into the syringe. For the Fusion Series syringe pumps, the pusher block moves to the right for infusion and to the left for withdrawal.

  • Page 11: Features

    Pump Fusion 100 pump components All Fusion Series pumps have a single lead screw through which the pusher block is threaded. The pusher block moves when the lead screw turns. The two guide rods keep the pusher block horizontal and perfectly perpendicular to the lead screw. The block-release button disengages the pusher block from the lead screw, which allows the pusher block to be easily moved to a new position as long as the button is held.
  • Page 12 Fusion Series User Manual Chemyx, Inc. Fusion 200-specific pump components In addition to having all of the same features and components of the Fusion 100, the Fusion 200 is capable of both infusion and withdrawal. Because of this difference, the Fusion 200 uses adjustable retaining brackets on the pusher block and the syringe-holder block that keep the syringe in place during both infusion and withdrawal.

  • Page 13: Controls

    Controls Front panel of pump The Fusion Series uses a bright, 4.25”, resistive, LCD touchscreen with an intuitive graphical user interface (GUI) for setting up detailed pump run methods. Buttons and entry fields within the GUI are all selectable using the touchscreen. As a resistive, LCD touchscreen, the interface can be operated with any object, not just the touch of a fingertip.

  • Page 14: Power And Data Connectors

    TTL port is also available, which provides easy control of the pump. Audible Alerts The Fusion series pumps have an audible beep that sounds when the pump powers on and when any of the pump control keys are pressed. A longer beep will sound if the pump has stalled.

  • Page 15: Specifications

    Fusion Series User Manual Chemyx, Inc. Specifications Specification Fusion 100 Fusion 200 Fusion 400 Available Movement Infusion Infusion / Withdrawal Infusion Modes 0.5 μL / 60 mL 0.5 μL / 60 mL 0.5 μL / 10 mL Syringe Sizes (Min/Max) 0.0001 - 1.5679 μL/min...

  • Page 16: Pump Setup

    Fusion Series User Manual Chemyx, Inc. Pump Setup Powering On/Off the Pump To provide power to the pump, an IEC 60320 C13 cable should be plugged into the power socket on the back of the pump. The cable should be plugged into an appropriate power source (110V or 220V) with proper grounding.

  • Page 17: Loading A Syringe

    Chemyx, Inc. Loading a Syringe The Fusion series can accommodate up to two syringes in the Fusion 100 and 200 models, while the Fusion 400 can house up to four syringes. The Fusion 200 can be expanded to 10 channels using the 10-Channel Syringe Rack accessory.

  • Page 18: Fusion 200

    Fusion Series User Manual Chemyx, Inc. Fusion 200 1. If necessary, move and tighten the infusion safety collar (see Adjusting the Safety Collar). 2. While pressing in the block release button, slide the pusher block all the way to the left.

  • Page 19: Fusion 400

    Adjusting the Safety Collar The Fusion series of pumps can generate a significant amount of linear force. While this is extremely useful for dispensing viscous fluids at higher flow rates, the higher linear force of the Fusion series can also potentially damage the syringes in use.

  • Page 20: Fusion 100 And 200

    Fusion Series User Manual Chemyx, Inc. Fusion 100 and 200 Adjusting the safety collar requires the use of the hex key, which is conveniently stored on the back left of the pump, right above the power switch. Loosening the hex screw on...

  • Page 21: Pump Operation

    Fusion Series User Manual Chemyx, Inc. safety bar clamp knob will keep the bar from moving. Pump Operation Selecting Items The LCD screen displays a graphical user interface (GUI) from which all the pump settings and run parameters are entered. Whenever an item is currently selected, the color scheme of the element typically turns to a darker shade of the color.

  • Page 22: Navigating Between Screens

    Chemyx, Inc. Navigating Between Screens All of the screens in the Fusion Series GUI exist in a hierarchy, with the Mode Selection screen at the top. Entering a new screen is performed simply by activating, or tapping, a button or icon that opens the new screen.

  • Page 23: Entering Letters

    Mode Selection Screen The Mode Selection screen is the top level menu for the Fusion series pumps. This screen allows users to select the Basic or Multi-Step modes, load a previously saved method, and adjust system settings or power settings.

  • Page 24: Basic Mode

    Fusion Series User Manual Chemyx, Inc. Basic Mode Basic Mode (or Single-Step Mode) is used for applications that do not require advanced automation, variable volume and rate settings, step looping, or ramping rates. The Basic Mode screen can be reached by tapping on the Basic icon on the Mode Selection screen.
  • Page 25 Fusion Series User Manual Chemyx, Inc. Tapping the Find Syringe button to the right of the Syringe editable field will open a new Syringe Library screen. Syringe manufacturers are found in the list on the left. Selecting a manufacturer will display all of...

  • Page 26: Target Transfer Volume

    Fusion Series User Manual Chemyx, Inc. Target Transfer Volume The Volume field represents the total volume of liquid that the pump will transfer (infusion or withdrawal) before stopping. The amount of time that the pump will run with the set target volume can be determined by dividing the volume by the flow rate.

  • Page 27: Start-Time Delay

    Fusion Series User Manual Chemyx, Inc. Caution Flow rate is often set for the needs of the experiment; however, users should be aware that setting high flow rates, especially for viscous fluids, may require high linear force beyond the limits of the pump which may cause the pump to stall out. Additionally, high flow rates can exert tremendous pressure within a syringe that may cause glass or plastic syringes to burst.

  • Page 28: Options

    Fusion Series User Manual Chemyx, Inc. Options Basic Mode Options screen Flow-Rate Units The flow-rate units determines which units of volume and time will be used for the Volume and Rate fields. It also determines the units for the Priming/Bolus Rate. This setting in Basic Mode also changes the units for Multi-Step Mode.

  • Page 29: Priming/Bolus Rate

    Chemyx, Inc. user selects “No” to the overwrite question, the screen will return to the Save As name field for the user to edit the name. The Fusion series pumps can store up to a maximum of 20 different methods.

  • Page 30: Multi-Step Mode

    Fusion Series User Manual Chemyx, Inc. Multi-Step Mode Multi-Step Mode is used for more advanced applications that may require numerous steps, multiple different volume and rate settings, step looping, or ramping rates. The Multi-Step Mode screen can be reached by tapping on the Multi-Step icon on the Mode Selection screen.

  • Page 31: Total Steps

    Fusion Series User Manual Chemyx, Inc. Total Steps The Total Steps field determines how many steps will occur in the method. Each step has independent values for Volume, Rate, Delay, and Infusion/Withdrawal. Upon entering the number of steps, the list on the left of the screen will update to show all of the steps. Each one of the steps will have their own set of parameters that will need to be set (see Step Parameters).

  • Page 32: Flow-Rate Units

    Fusion Series User Manual Chemyx, Inc. Flow-Rate Units Tapping the Options button opens a screen that allows flow-rate units to be set. The flow-rate unit determines which unit of volume and time will be used for the Volume and Rate fields for all of the steps.

  • Page 33: Infusion/Withdrawal (Fusion 200 Only)

    Fusion Series User Manual Chemyx, Inc. The value entered can be any integer or decimal within the limits for the syringe. The min/max volume limits can be found in the blue bar at the top of the screen when the Volume field is selected.

  • Page 34: Static Flow Rate

    Fusion Series User Manual Chemyx, Inc. The rate can be stated with four different units: mL/min, mL/hr, μL/min, and μL/hr. Adjusting the units will affect the min/max limits for the Rate field. The units for rate can be set in Options during the multi-step setup.

  • Page 35: Step Loop

    Fusion Series User Manual Chemyx, Inc. Step Loop The Step Loop field determines how many additional times to sequentially perform the particular step. This is the lowest-level loop that iterates whenever that particular step is reached and completed. Example In a multi-step method of five steps (1, 2, 3, 4, 5) where Step 4 has a Step Loop of “2”. The method will run the following steps sequentially: 1, 2, 3, 4, 4, 4, 5.

  • Page 36: Running The Pump

    Fusion Series User Manual Chemyx, Inc. Running the Pump The pump’s actions are controlled by the Stop, Pause, and Start keys on the front. In order to start a run, the screen should be showing either the Basic Mode (for basic runs) or Multi-Step Mode (for multi-step runs) main screens.

  • Page 37: Basic Mode System Status

    Fusion Series User Manual Chemyx, Inc. Upon starting a pump run, the screen will switch to the System Status screen. There are two different versions of this screen depending upon whether the pump was started in Basic Mode or Multi-Step Mode.

  • Page 38: Multi-Step Mode System Status

    Fusion Series User Manual Chemyx, Inc. Multi-Step Mode System Status Multi-Step Mode System Status screen The System Status screen for Multi-Step Mode highlights the current activity of the pump during a step of the multi-step method. The All Loop field at the top of the screen shows the current loop and the number of total loops for the multi-step method.
  • Page 39 Fusion Series User Manual Chemyx, Inc. For the Rate field, Rate 1 (first number) and Rate 2 (second number) are both displayed. If the two numbers are the same, the current step is running a static flow rate. If they are different, the...

  • Page 40: Load Run

    Fusion Series User Manual Chemyx, Inc. Load Run The Load Run screen allows users to recall Basic Mode methods that were saved in the Options screen. The Load Run screen can be reached by tapping on the Load folder icon on the Mode Selection screen.

  • Page 41: System Settings

    The Fusion Series pumps provide two possible baud rates: 9600 and 38400. Serial connections using the USB-B port on the back of the pump should use the faster baud rate of 38400.

  • Page 42: Power Settings

    Selection screen. Motor Power The Fusion Series pumps can generate up to a maximum of 50 lbs (35 lbs for the Fusion 100) of linear force. Some syringes made of more fragile materials may not be able to withstand the full linear force of the pump.

  • Page 43: Lcd Power-Off Time

    Fusion Series User Manual Chemyx, Inc. LCD Power-Off Time This setting sets the amount of time until the LCD display goes to sleep after the last interaction with the pump controls. When the display goes to sleep, the pump is still on. It will continue to...

  • Page 44: Pump Control By Computer

    Fusion Series User Manual Chemyx, Inc. Pump Control by Computer The Fusion Series syringe pumps can be controlled with an external computer using an RS232 serial connection. Cable Requirements Two types of connections are available for external computer control: DB9 and USB, cables for each are available for purchase through Chemyx (call 1-281-277-5499 or visit www.chemyx.com/contact-us).

  • Page 45: Connecting

    Fusion Series User Manual Chemyx, Inc. Connecting Most computer operating systems (e.g., Windows, macOS, Linux, etc.) have built-in terminal programs that can be used to type in the commands that run the pump. On Windows XP and earlier, HyperTerminal can be used to control to the pump. On macOS and Linux, the screen command in Terminal can also be used to control the pump.
  • Page 46 Fusion Series User Manual Chemyx, Inc. help Displays a list of available commands. start Starts the pump with the currently set parameters. The parameters that will run depend upon which mode (Basic Mode or Multi-Step Mode) the pump touchscreen is showing.
  • Page 47 Fusion Series User Manual Chemyx, Inc. sets whether the pump is infusing or withdrawing (Fusion 200 only) and the number of steps used in Multi-Step Mode. X must be an integer or decimal (up to five decimal places) within the volume limits for the syringe (See read limit parameter command).
  • Page 48 Fusion Series User Manual Chemyx, Inc. spaces. The number of steps will be automatically adjusted to the number of values entered (i.e., entering only three values will change the number of steps to three). Example set time 1.0,0.5,1.3 Returns target time and rate (in Basic Mode).
  • Page 49 Fusion Series User Manual Chemyx, Inc. set delay X,X,X,... Sets the start time delay (in minutes) prior to each step of a pump run. Upon starting the a step, the pump will wait until the delay time elapses before starting to transfer volume.
  • Page 50 Fusion Series User Manual Chemyx, Inc. Returns: rate units, syringe inner diameter, transfer rate, priming rate, time (in whole number minutes), transfer volume, time delay (in whole number minutes) status Returns the current status of the pump. The returned integer correlates to the status of the pump.

  • Page 51: Chemyx Pump Controller Programs

    Chemyx Pump Controller Programs In addition to the manual control of the pumps through a terminal, Chemyx provides pump controller programs as an unsupported open-source applications written in Python, a LabVIEW package, and a MATLAB package. More information on these tools can be found on the website...

  • Page 52: Pump Maintenance

    Pump lubrication should be performed every three months. Approved Lubricants ● Engine/motor oil ● Silicone oil ● Machine oil ● Tooling oil or “WD-40” ● Lithium-based grease Please contact Chemyx (call +1 281-277-5499 or visit www.chemyx.com/contact-us) to confirm the safe use of a different lubricant.

  • Page 53: Replacing The Fuse

    In the event of a power surge, the fuse in the syringe pump may burn out. If a fuse burns out, the pump will be unable to receive power. Chemyx syringe pumps come with a complimentary backup fuse inside the pump. Please review the following instructions for accessing the fuse holder and replacing the burned-out fuse.

  • Page 54: Pump Accessories

    Connecting the Foot Switch The Chemyx Foot Switch for Fusion Series pumps has a USB-B cable that plugs into the TTL port on the back of the pump. Using the Foot Switch To use the foot switch, the pump must be in either the Basic Mode or Multi-Step Mode screens.

  • Page 55: 10-Channel Syringe Rack (Fusion 200 Only)

    Fusion Series User Manual Chemyx, Inc. 10-Channel Syringe Rack (Fusion 200 only) 10-Channel Syringe Rack Chemyx 10-Channel Syringe Rack for the Fusion 200 provides users with an expansion of the number of syringes that can be used simultaneously. The 10-channel syringe rack can accommodate up to 10 syringes with volumes from 1 μL to 10 mL.

  • Page 56: Attaching The Rack

    Fusion Series User Manual Chemyx, Inc. Attaching the Rack 10-Channel Syringe Rack components...
  • Page 57 Fusion Series User Manual Chemyx, Inc. 1. Completely unscrew the bracket clamp knob for the plunger retaining bracket and remove the bracket. Replace the retaining bracket with the 10-channel plunger retaining bracket. Screw the bracket clamp knob back on and tighten to secure the 10-channel plunger retaining bracket.

  • Page 58: Loading Syringes

    Fusion Series User Manual Chemyx, Inc. Loading Syringes 1. If necessary, move and tighten the infusion safety collar (see Adjusting the Safety Collar). 2. While pressing in the block-release button, slide the pusher block all the way to the left.

  • Page 59: Appendices

    Fusion Series User Manual Chemyx, Inc. Appendices Appendix A: Syringe Volume/Diameter Reference Table Chemyx Stainless Steel Size Diameter 6.00 mL 9.53 mm 20.00 mL 19.13 mm 50.00 mL 28.6 mm BD Glass Size Diameter 0.50 mL 4.64 mm 1.00 mL 4.64 mm...
  • Page 60 Fusion Series User Manual Chemyx, Inc. 20.00 mL 19.13 mm 30.00 mL 21.69 mm 60.00 mL 26.72 mm Hamilton Glass Size Diameter 0.50 μL 0.10 mm 1.00 μL 0.15 mm 2.00 μL 0.21 mm 5.00 μL 0.33 mm 10.00 μL 0.48 mm...
  • Page 61 Fusion Series User Manual Chemyx, Inc. 2.00 mL 9.20 mm 3.00 mL 10.30 mm 5.00 mL 12.20 mm 10.00 mL 15.00 mm 20.00 mL 19.00 mm 30.00 mL 22.50 mm 50.00 mL 25.50 mm 100.00 mL 34.00 mm JMC Air-Tite Plastic...
  • Page 62 Fusion Series User Manual Chemyx, Inc. 5.00 mL 9.50 mm Nipro Size Diameter 1.00 mL 4.71 mm 3.00 mL 9.50 mm 5.00 mL 12.91 mm 10.00 mL 15.40 mm 20.00 mL 19.30 mm 30.00 mL 23.40 mm 50.00 mL 29.00 mm...
  • Page 63 Fusion Series User Manual Chemyx, Inc. 3.00 mL 8.99 mm 5.00 mL 11.70 mm 10.00 mL 14.70 mm 20.00 mL 19.58 mm 30.00 mL 22.70 mm 50.00 mL 29.00 mm Ranfac Size Diameter 2.00 mL 9.12 mm 5.00 mL 12.34 mm 10.00 mL...
  • Page 64 Fusion Series User Manual Chemyx, Inc. Size Diameter 1.00 mL 4.65 mm 3.00 mL 8.94 mm 6.00 mL 12.70 mm 12.00 mL 15.90 mm 20.00 mL 20.40 mm 35.00 mL 23.80 mm 50.00 mL 26.60 mm Terumo Size Diameter 1.00 mL 4.73 mm...
  • Page 65 Fusion Series User Manual Chemyx, Inc. 50.00 mL 29.10 mm Size Diameter 1.00 mL 4.70 mm 2.00 mL 6.40 mm 3.00 mL 9.30 mm 6.00 mL 13.10 mm 12.00 mL 15.40 mm 25.00 mL 21.00 mm 30.00 mL 23.00 mm 50.00 mL...
  • Page 66 Fusion Series User Manual Chemyx, Inc. 20.00 mL 20.40 mm 35.00 mL 23.80 mm 60.00 mL 26.60 mm...

  • Page 67: Appendix B: Available Parts

    Fusion Series User Manual Chemyx, Inc. Appendix B: Available Parts Fusion Series Syringe Pumps Item Description Part Number Fusion 100 Touch 07100 Fusion 200 Touch 07200 Fusion 400 Touch 07400 Options and Accessories Item Description Part Number Foot Switch (w/ USB-B connection cable)

  • Page 68: Appendix C: Contact Information

    Fusion Series User Manual Chemyx, Inc. Appendix C: Contact Information Chemyx Inc. 10905 Cash Rd. Stafford, TX 77477 Webpage: www.chemyx.com Contact Us: www.chemyx.com/contact-us Phone: +1 281-277-5499 Fax: +1 281-277-5499 Office Hours: Monday–Friday, 8:30 am – 5 pm (Central Time) For more articles and tutorial videos, visit: www.chemyx.com/support...

This manual is also suitable for:

Fusion 200Fusion 100Fusion 400

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