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6
Instruction Manual
4. Preparation of the Stock Solutions
4.1
Allow all vials to warm to room temperature before opening.
4.1.1
For the preparation of a 20 µM stock solution of EdU (2X EdU), add the
appropriate amount of aqueous solution (1X PBS) to EdU (component A)
according to table 3 and mix until the compound is completely dissolved. After
use, store any remaining solution at -20°C. When stored as directed, this stock
solution is stable for up to one year.
EdU-based HTS Kit
1 well plate
2 x 96 well plates
Table 3: Amounts of aqueous solution needed to dissolve EdU to a final concentration of 20 µM.
4.1.2
For the preparation of a stock solution of the buffer additive, add the appropriate
amount of deionized water (see table 4) to the component E and mix until the
compound is dissolved completely. After use, store any remaining solution at -
20°C. When stored as directed, this stock solution is stable for up to 6 months.
We recommend preparing aliquots to avoid repeated thaw and freeze cycles!
EdU-based HTS Kit
1 well plate
2 x 96 well plates
Table 4: Amounts of aqueous solution needed to dissolve the buffer additive to the final work
solution.
4.1.3
For the preparation of a stock solution of the buffer additive, add the appropriate
amount of deionized water (see table 4) to the component E and mix until the
compound is dissolved completely. After use, store any remaining solution at -
20°C. When stored as directed, this stock solution is stable for up to 6 months.
We recommend preparing aliquots to avoid repeated thaw and freeze cycles!
5. Labeling of Cells with EdU
5.1
Suspend the cells in an appropriate tissue culture medium to obtain optimal cell
growth conditions. Please note that the growth of the cells during incubation
decelerates, if the temperature changes or the cells are washed prior to
incubation with EdU.
20X EdU solution
1 mL
2 mL
Buffer additive
Dilution volume of deionized water
(solide)
100 mg
200 mg
In dilution Volume for 2X EdU
solution in PBS
9 mL
18 mL
1 mL
2.5 mL
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