Materials Provided With The Kit And Storage Conditions - PromoKine PK-CA724-488HTS Instruction Manual

4 Instruction Manual

1. Materials provided with the Kit and Storage Conditions

Amount for 2 x 96
Vial-label
assays/well plates
Component A
yellow
Component B
red
Component C
orange
Component D
green
Component E
blue
Component F **
grey
Table 2: Contents of the kit and storage conditions.
Cell Proliferation Kit III (EdU; HTS) ready for 2 x 96 well plate assays:
Product number
PK-CA724-488HTS
PK-CA724-555HTS
PK-CA724-594HTS
PK-CA724-647HTS
The Kit contains sufficient material for 2 x 96 well plate assays.
This kit is stable up to 1 year after receipt, when stored as directed.
1 Stable if stored at -20°C in the dark for at least one year.
* When dissolved the component E has to be kept at -20°C for long-term storage.
** Cautions: The rinse buffer (Component F): contains hazardous components. Use with appropriate
precautions. Keep away from acids to avoid dangerous gases.
Handle reagents containing the rinse buffer using equipment and practices appropriate for the hazards
posed by such materials. Use gloves. Dispose of the reagents in compliance with all related local
arrangements. For the correct handling we refer you to the MSDS which can be downloaded from our
webpage www.promocell.com.
This solution is stored at RT and will crystallize at lower temperatures. If crystallized, the solution has to
be brought to RT, mixed thoroughly and can then, once homogenously dissolved, be used without
further considerations. The activity of this compound is not affected hereby.
2 mL 5-Ethynyl-deoxyuridine (5-EdU)
•
6-FAM Azide
488HTS)
•
5-TAMRA-PEG3-Azide
CA724-555HTS)
130 µL •
5/6-Sulforhodamine101-
PEG3-Azide
•
(PK-CA724-594HTS)
•
Eterneon-Red 645 Azide
(PK-CA724-647HTS)
20 mL Reaction buffer
1 mL Catalyst solution
200 mg Buffer additive
6 ml Rinse buffer (10X)
20X EdU in PBS
2 mL
6-FAM Azide (λ
2 mL
5-TAMRA-PEG3-Azide (λ
5/6-Sulforhodamine 101-PEG3-Azide
2 mL
(λ
abs
2 mL
Eterneon Red 645-Azide(λ
Component
Component long term
1 (PK-CA724-
1 (PK-
1
1
Used fluorescent dye
=496/λ
abs em
abs
=584/λ =603)
em
abs
Kit storage
storage
-20°C
2 - 8°C
Dark & Dry
-20°C
dark
Do not
freeze
RT
RT
RT /
-20°C*
RT
RT
=516)
=546/λ =579)
em
=643/λ =662)
em