Example Data - PromoKine PK-CA724-488HTS Instruction Manual

10 Instruction Manual
Product number
PK-CA724-488HTS
PK-CA724-555HTS
PK-CA724-594HTS*
PK-CA724-647HTS*
Table 8: Emission and excitation maxima of the available dyes. * Currently not available.
11. Example of the data derived from an experiment using the EdU-based HTS Kit:
Figure 1. Detection of EdU incorporation depending on cell number and EdU incubation time.
HeLa cells were seeded in a transparent 96well cell culture plate with indicated cell
numbers per well. After 42 hours cells were incubated with or without 10 μM EdU for
2, 4 or 6 h and subsequently EdU incorporation was detected using the EdU-based Cell
Proliferation Assays III (EdU; HTS) and a fluorescence plate reader. Mean and SD values
from quadruplicates are shown.
Dye
6-FAM Azide
5-TAMRA-PEG3-Azide
5/6-Sulforhodamine 101-PEG3-Azide
Eterneon Red 645-Azide
Excitation Emission
(nm) (nm)
496 516
546 579
584 603
643 662
Filter
Green
Violet
Orange
Red