Applied Biosystems 3730xl User Manual page 31

3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide
8. Fill, then assemble the reservoirs.
Buffer reservoir assembly
1. Add 80 mL 1X run buffer to the
Buffer reservoir.
2. Assemble the reservoir assembly as
shown below.
6
Retainer
1
Septum
2
Reservoir cap
3
Reservoir
4
Heated plate base
5
Power cable
6
9. To prevent damage to the capillary array, inspect each reservoir for the following
conditions.
• Septa fit snugly and flush on the reservoir cap
• Rubber gasket surrounding the reservoir cap is seated correctly
Rubber gasket not seated correctly
1
Chapter 2 Prepare the instrument
Prepare buffer and fill the reservoirs
Water and waste reservoir assemblies
1. Add 80 mL high-quality deionized
water to each reservoir.
2. Assemble each reservoir assembly
as shown below.
1
2
3
4
5
Retainer
1
Septum
2
Reservoir cap
3
Reservoir
4
Plate base
5
2
1
2
3
4
5
1
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