Applied Biosystems 3730xl User Manual
  1. Manuals
  2. Brands
  3. Applied Biosystems Manuals
  4. Measuring Instruments
  5. 3730xl
  6. User manual

Applied Biosystems 3730xl User Manual

Dna analyzer
Hide thumbs
Table of Contents

Advertisement

Quick Links

Download this manual
3730xl DNA Analyzer
USER GUIDE
3730xl Data Collection Software 5
™
Windows
10 Operating System
Catalog Number A39079
Publication Number 100077621
Revision A
For Research Use Only. Not for use in diagnostic procedures.

Advertisement

Table of Contents
loading

Related Manuals for Applied Biosystems 3730xl

Summary of Contents for Applied Biosystems 3730xl

  • Page 1 3730xl DNA Analyzer USER GUIDE 3730xl Data Collection Software 5 ™ Windows 10 Operating System Catalog Number A39079 Publication Number 100077621 Revision A For Research Use Only. Not for use in diagnostic procedures.
  • Page 2 Manufacturer: Life Technologies Holdings Pte Ltd | Block 33 | Marsiling Industrial Estate Road 3 | #07-06, Singapore 739256 The information in this guide is subject to change without notice. DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, THERMO FISHER SCIENTIFIC INC. AND/OR ITS AFFILIATE(S) WILL NOT BE LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT.

  • Page 3: Table Of Contents

    Check the active spectral calibration ..........36 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...
  • Page 4 Move a plate in the Input stack during a run ........64 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...
  • Page 5 Analysis protocol parameters ..........89 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...
  • Page 6 Change polymer type ............120 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...
  • Page 7 Power and communication connections ......... . 145 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...
  • Page 8 Biological hazard safety ............164 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...
  • Page 9 Index ..............167 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 10: Chapter 1 Instrument And Software Description

    Workflow for a typical run ..........26 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 11: Overview

    The user provides inadequate maintenance · The equipment is used in a manner not specified by the manufacturer (Thermo Fisher Scientific) IMPORTANT! Observe current good laboratory practices when using this instrument. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 12: Parts Of The Instrument

    Oven door (The detector cell and capillary array are inside this compartment. ) Stacker (automated plate handler) Stacker door indicator light Power button and status lights Tray button Buffer, water, and waste reservoirs 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 13: Instrument Interior Components

    Detection cell (behind door) Capillary array Figure 3 Polymer delivery pump (PDP) Pump block Polymer supply tube Polymer reservoir Anode buffer jar Capillary array Interconnect tube Lower polymer block 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 14: Instrument Front Panel Indicators

    • The instrument has detected a problem. See Appendix A, Solid Red “Troubleshooting“. • Restart the instrument and computer. See “Power on the computer and the instrument“ on page 27. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...
  • Page 15 • Out Stack— Empty, no plates in stack • In Stack—Empty, no plates in stack Solid Red • Out Stack— Full, remove plates before you can start another run 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 16: Consumables And Usage Limits

    Life Technologies instructions for the instrument system, software, or accessories. Please see your specific service contract or limited product warranty for exact language regarding coverage and ask your Life Technologies representative if you have further questions. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 17: Computer And Software Requirements

    Do not change the static IP settings. For minimum computer requirements, see “Instrument specifications“ on page 142. IMPORTANT! Do not rename the computer after the 3730xl Data Collection Software 5 is installed. The instrument computer has been assigned a unique name. Changing the name may cause the software to malfunction.

  • Page 18: Preparing The Instrument

    • For fragment analysis, the software uses the internal size standard to assign a fragment size and a sizing quality value to each peak. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 19: Overview Of The Software

    Use the Navigation pane (Figure 4) to set up your run, view and interrogate results, or to determine the amount of free space remaining in your database. Figure 4 Navigation pane 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...
  • Page 20 Run History—View previous runs. See “View data from a completed run (Run History)“ on page 71. Instrument name —View the status of the instrument. See “Check instrument status“ on page 67. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...
  • Page 21 Spectral Calibration Viewer—View the spectral calibration from a specific run. Reextraction—Check autoextraction success or failure and re-analyze samples with different settings. See “Check the autoextraction status and manually re-extract“ on page 72. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 22: Use The Instrument With Thermo Fisher Connect

    • Receive instrument status email notifications. • View instrument status on InstrumentConnect or a mobile device. For more information, see Chapter 3, “Use the instrument with Thermo Fisher Connect“. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 23: Secondary Analysis Software

    Sizing Analysis Module Peak Performs peak sizing. Scanner ™ Software Microsatellite Analysis Analyzes a mixture of DNA fragments, separated by size, and Software determines the microsatellite alleles present in the sample. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 24: Data Collection Software Terminology

    10 operating system. Older versions may or may not be able to analyze data files that are generated by the 3730xl Data Collection Software 5. Contact Support for information on obtaining the latest versions of software if you did not buy these applications with the instrument.
  • Page 25 (injections) may occur for a plate. For example, a 96-well plate on a 48-capillary instrument will require 2 runs (injections) to sample all wells on the plate. Injection 1 Injection 2 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 26: Workflow For A Typical Run

    View data from the run (page 69) Create a plate record (page 55) ▼ Review results in secondary analysis software on Thermo Fisher Connect or on desktop software (page 23) 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 27: Chapter 2 Prepare The Instrument

    In the User Name field, enter your user name. b. In the Password field, enter your password. c. Click OK. 3. Close the oven door. 4. Close the stacker door. 5. Close the instrument door. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 28: Start The Software

    Chapter 2 Prepare the instrument Start the software Start the software 1. In the desktop, double-click the 3730xl Data Collection Software 5 icon. The Service Console dialog box is displayed and services start. Note: If the dialog box is not displayed, click the Service Console tab in the ™...

  • Page 29: Prepare Buffer And Fill The Reservoirs

    • 3730 Running Buffer (10X) , 20 mL • Graduated cylinder, 250-mL • Gloves, silicone-free, powder-free 1X run buffer • 2–8°C for up to 1 month storage • Room temperature for 1 week 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 30: When To Change The 1X Run Buffer

    5. Disassemble each reservoir assembly then empty the contents of the reservoirs into an aqueous waste container. 6. Clean each reservoir using deionized water. 7. Dry the reservoirs using lint-free wipes. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...
  • Page 31 9. To prevent damage to the capillary array, inspect each reservoir for the following conditions. • Septa fit snugly and flush on the reservoir cap • Rubber gasket surrounding the reservoir cap is seated correctly Rubber gasket not seated correctly 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 32: Place Reservoirs Into The Instrument

    Plate base cable 2. Move the cable out of the way of the autosampler. Move the buffer reservoir to the Buffer position (left). Buffer reservoir Left reservoir position Middle reservoir position 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 33: Fill The Anode Buffer Jar

    Add 5 mL 1X run buffer to the anode buffer jar. Overflow hole b. Tilt the anode buffer jar 90°. c. Rotate the jar to rinse the interior with buffer. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 34: Check The Polymer Level

    1 week. See “Replenish or change polymer type“ on page 119. IMPORTANT! Allow polymer to reach room temperature before installing on the instrument. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 35: Check For Bubbles In The Pump System

    IMPORTANT! When this wizard runs, it introduces polymer into the anode buffer jar. Replace the anode jar buffer after you run this wizard. See “Fill the anode buffer jar“ on page 33. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 36: Check The Active Spectral Calibration

    2. Select the dye set for the run. 3. If the active spectral calibration is not appropriate for your application, see “Select a previous calibration as the active spectral calibration“ on page 111. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 37: Prepare And Load Sample Plates

    After you load samples in plates, see “Seal and assemble plates“ on page 41. Default injection positions—48-capillary arrays 96-Well plate, 2 injections are required to sample all wells on the plate. 48 capillaries Injection 1 Injection 2 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 38: 384-Well Plate, 48 Capillaries

    2 injections from each quadrant are required to sample all wells on the plate. 48 capillaries Injection 1 Injection 2 No injection Quadrant 1 Quadrant 2 Quadrant 3 Quadrant 4 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 39: Default Injection Positions-96-Capillary Arrays

    Chapter 2 Prepare the instrument Default injection positions—96-capillary arrays Default injection positions—96-capillary arrays 96-Well plate, 1 injection 96 capillaries 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 40: 384-Well Plate, 96 Capillaries

    384-Well plate, 1 injection from each quadrant is required to sample all wells on the plate. 96 capillaries Injection 1 No injection Quadrant 1 Quadrant 2 Quadrant 3 Quadrant 4 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 41: Seal And Assemble Plates

    Figure 8 Examples of bad and good septa alignment Septum and well are not aligned Septum and well are not aligned Septum and well are not aligned Septum and well are properly aligned 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 42: Prepare The Plate Assemblies

    Seal and assemble plates Prepare the plate 1. Prepare the plate assembly as shown in the following figures. assemblies Plate retainer Plate septum Septum-sealed sample plate Black plate base Assembled components 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...
  • Page 43 3. Make sure when you assemble a plate that the retainer clip is flush with the plate base. A simple way to ensure that they are flush is to run your finger along the edge. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 44: Important Heat Seal Recommendations

    3. Place the plate assemblies into the stacker. Ensure that the notched corner of the plate assembly is at the rear right corner of the stacker. IMPORTANT! Do not place more than 16 plates in the stacker. Notched corner 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...
  • Page 45 Chapter 2 Prepare the instrument Place plate assemblies into the instrument 4. Close the metal In Stack tower door. 5. Close the Stacker drawer. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 46: Chapter 3 Use The Instrument With Thermo Fisher Connect

    View instrument status on InstrumentConnect or a mobile device. IMPORTANT! The instrument communicates with the computer by Ethernet connection. Do not make any changes to the computer ethernet/internet connections during a run or during calibration. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 47: Register And Obtain A Thermo Fisher Connect Account

    3. Fill in all information, then click Create account. Connect the instrument to your Thermo Fisher Connect account 1. In the navigation pane of the Data Collection Software, select Instruments4 ga37304 Thermo Fisher Connect. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 48: Set Up The Data Storage Location And Email Notifications

    User ID is the active ID. 4. Select the Receive Notifications checkbox. Run status emails will be sent to your Thermo Fisher Connect account email address. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 49: Monitor A Run From Instrumentconnect

    Note: The Add an Instrument function is not supported for the instrument. See “Connect the instrument to your Thermo Fisher Connect account“ on page 47. 3. Click the instrument to display instrument status. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 50: Monitor A Run From A Mobile Device

    1. On your mobile device, download the InstrumentConnect from the Apple Store ™ or from Google Play. 2. On your mobile device, launch InstrumentConnect. 3. Touch the instrument to monitor. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 51: View Notifications From The Instrument On The Cloud

    View notifications from the instrument on the Cloud 1. In any screen in the Thermo Fisher Cloud, click 2. Click a notification, then click Dismiss or Dismiss all to dismiss the notification. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 52: For More Information On Using Instrumentconnect

    • Assign administrator role to one or more users. • Remove an account from an instrument. • Disconnect the instrument from InstrumentConnect. • Change the instrument name. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 53: Manage The Users And Administrators Of Your Instrument

    To... Do this... Assign the Admin role to an additional Select the Admin checkbox, then click user Close. Remove a user Click , then click Confirm. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 54: Disconnect Individual Users From An Instrument

    You can remove a user from the instrument. However, doing so deletes the user data from the instrument. For more information, see “Manage the users and administrators of your instrument“ on page 53. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 55: Chapter 4 Create Results Groups And Plate Records Using

    4. Click the Analysis tab, then select the Analysis Type. For more information, see “Overview of results groups“ on page 79. For information on autoanalysis, see the documentation for the secondary analysis software. 5. Click OK. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 56: Create A Plate Record

    • Select an existing results group from the list. • Click New or Duplicate to create a new results group For information on results groups, see “Overview of results groups“ on page 79. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...
  • Page 57 • 96-well plate: Select Fill Down. the Plate Manager to copy information from an existing • 384-well plate: Fill down Special protocol to a new protocol. (96 Cap). 11. Click OK. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...
  • Page 58 View data from the run ..........69 ■ Check the autoextraction status and manually re-extract ....72 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 59: Chapter 5 Run The Instrument

    You can change this default injection order in two places. • In a new plate record before a run • In the Run Scheduler (See “Change injection order of plate quadrants“ on page 63) 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...
  • Page 60 Well B4 Note: With a 384-well plate and a 48-capillary array, you can change the run order of the main quadrant (bold numbers above) but not the injection numbers. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 61: Run The Instrument In Auto Mode

    Run Scheduler. 2. At the top of the screen, select Instrument4Instrument name Run Mode, then select Manual. Note: The Instrument menu is available only when the Run Scheduler is displayed. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...
  • Page 62 You can also change the Type of Search to Advanced and enter more specific plate criteria to search for. For more information, see “Search for a plate record“ on page 77. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 63: Change Injection Order Of Plate Quadrants

    • “Default injection positions—48- capillary arrays“ on page 37 • “Default injection positions—96- capillary arrays“ on page 39 • “Default injection order on plates“ on page 59 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 64: Move A Plate In The Input Stack During A Run

    Stop after the current run Skip to next run (stops the current run and begins next scheduled run) Pause the current run Resume after pause (start the next scheduled run) 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 65: Monitor The Status Of The Run

    Plate has been validated and will not be run. For more information, see the following sections. • “Display the Event Log“ on page 135 • “Plate validation fails when you start a run“ on page 137 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 66: Add Reinjections To A Plate Record

    2. Select a plate record, then click Edit. 3. Select Edit4Add Sample Run. Columns for Results Group 2 and Instrument Protocol 2 are added to the plate record. 4. Specify the settings for the new injection. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 67: Check Instrument Status

    Displays errors that have occurred during the current run. Some of the error messages provide information for service engineers. A “fatal” error usually requires that you restart the Data Collection Software. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 68: View The Event Log

    2. To delete error messages, select all error messages, then click Clear Errors. Error messages are retained in the Runviewer event log files that are stored in The system status indicator flashes red until all errors are cleared. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 69: View Data From The Run

    1. Click-drag the mouse over the area of interest. 2. Release the mouse, then click to expand the view. 3. Click to return to full view. Click individual colors to view or hide them. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...
  • Page 70 1. To expand the view, click-drag the mouse over the area of interest. 2. Click to return to full view. Display or hide color Click individual colors in the color bar to view or hide the color in the Array View. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 71: View Electrophoresis Conditions (Ept)

    3. Select the run of interest, then select any of the icons that are listed below. Run History views Icon EPT Viewer Spatial Calibration Viewer Capillary Viewer Array Viewer Spectral Calibration Viewer Reextraction 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 72: Check The Autoextraction Status And Manually Re-Extract

    All completed runs from the plate are listed and can be reextracted. Pending runs from the plate are not listed. 3. Select a run. 4. Click (Reextraction) in the navigation pane. The Reextraction window opens. Click-drag the Result column to display messages. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...
  • Page 73 “sample01.2.ab1”. Reextracted sample files are saved in the original folder that data was extracted to, unless you modify the results group settings. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 74: Results Groups

    384-well plates or 96-well plates (48-capillary array). For more information, see “Default injection order on plates“ on page 59. Figure 10 Plate attributes and injection order 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...
  • Page 75 Figure 12 Plate record required fields Fields required by Data Collection Software Fields required by secondary analysis software (this example shows fields required by GeneMapper Software) ™ 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 76: Create And Manage Plate Records

    3. Add replicate injections with the same or different results group or instrument protocol: Select Edit4Add Sample Run. Columns for Results Group 2 and Instrument Protocol 2 are added to the plate record. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 77: Search For A Plate Record

    Between search condition is selected.) 4. Click Search. Add plate records to the search list Select the Append Results checkbox. Remove plate records from the Click Clear or Clear All. search list 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 78: Import A Plate Record

    1. In the Plate Manager, click Import, then navigate to the plate record TXT file to import. 2. Select the file of interest, then click Open. A progress screen opens. Click OK when the import is complete. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 79: Overview Of Results Groups

    • Project • Size Standard ACGT sequence • Project Template • Panel • Specimen • Analysis method • Analysis Protocol • User Defined fields 1–3 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 80: Create And Manage Results Group

    4. Click the Analysis tab, then select the Analysis Type. For more information, see “Overview of results groups“ on page 79. For information on autoanalysis, see the documentation for the secondary analysis software. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...
  • Page 81 79 for default storage location. Custom naming 1. Select the Use Custom Location checkbox, then click conventions Browse and navigate to a location. 2. Click Test to ensure the location is accessible. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 82: Specify Custom Sample File Naming In A Results Group

    The <ext> attribute is determined by the Analysis Type you select in the Analysis tab. Prefix Text you can type. Delimiter Symbols you can include in the file name: dash (-), dot (.), underscore (_), plus (+), dollar ($). 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...
  • Page 83 • Subfolder Delimiter (Run File Name Format only) • User Name (the user logged into the software if you are using the AB Navigator Software) • Well Position 4. Click OK. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 84: Modules, And Analysis Protocols

    Copies information from an existing protocol to a new protocol. Delete Deletes a saved protocol. Import Imports an XML file. Export Saves as an XML file in E:\Users\username\Documents . 3. Click OK. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 85: Create An Instrument Protocol

    5. Select the run module and dye set for the protocol (see Appendix C, “Run modules“). ™ Note: If you will use BigDye XTerminator Purification Kit for sequencing reaction clean-up, select the run modules marked as 'BDx'. 6. Click OK. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 86: Create Run Module

    4. Select Regular in the Type dropdown list. 5. In the Template dropdown list, select a template module. 6. Edit the settings as needed. See “Run module parameters“ on page 87. 7. Click OK. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 87: Run Module Parameters

    • Mixed Bases—(Optional) Mixed base identification and the percent value of the second highest to the highest peak. • Clear Range—The clear range to be used based on base positions, sample quality values, and/or number of ambiguities (Ns) present. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 88: Create And Manage Analysis Protocols

    Sequencing Analysis Software or SeqScape Software will not be performed. Import Imports an XML file. Export Saves as an XML file in E:\Users\username\Documents . 3. Click OK. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 89: Create An Analysis Protocol

    PHD 1 file that can be used with other software. Basecaller and Dye Set Select the appropriate Basecaller and DyeSet/Primer based on the chemistry and capillary array length you are using. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...
  • Page 90 • Use identification of N cells—Sets a window with a specified number of allowed ambiguous base calls (Ns) by removing bases until there are < X number of Ns per Y number of bases. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 91: Chapter 8 Calibrate The Instrument

    Note: You do not need to fill the capillaries each time you perform a spatial calibration. 3. Click Start. 4. Evaluate the calibration. See “Evaluate the spatial calibration data“ on page 92. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 92: Evaluate The Spatial Calibration Data

    2. Verify that an orange cross appears at the top of each peak in the profile. Expand the profile if needed. See “Magnify the spatial profile“ on page 96. Figure 14 Peak that does not have orange cross No orange cross 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...
  • Page 93 Note: Higher values are acceptable if a corresponding gap in the capillaries in the detection cell are visible. Examine every position for the capillary array. 4. Click Accept or Reject. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 94: Examples Of Passing And Failing Spatial Profiles

    Chapter 8 Calibrate the instrument Perform spatial calibration Examples of passing and failing spatial profiles Figure 15 Typical passing profile 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...
  • Page 95 Figure 16 Passing profiles with higher peak heights at the beginning and the end of the profile Figure 17 Passing profile with low signal at the beginning of the profile 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 96: Magnify The Spatial Profile

    Figure 18 Passing profile with high background at the beginning of the profile Figure 19 Failing spectral profile Magnify the 1. Click-drag the cursor to create a box around the area of interest. spatial profile 2. Release the mouse button. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 97: Perform Spectral Calibration For Sequencing And Fragment Analysis

    • After the capillary array is changed or replaced • If you see a decrease in spectral separation (pull-up and/or pull-down peaks) • If you alter any condition (dye set, array type, array length, or polymer type) 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 98: Supported Dye Sets And Calibration Standards

    – Heat-seal with gray plate base ™ • Hi-Di Formamide • Heated block or thermal cycler • Container with ice • Centrifuge with microplate adapter • Microcentrifuge • Vortex • Gloves 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 99: Prepare The Spectral Calibration Standard

    Run Module for the installed Calibration type Chemistry capillary length Matrix Spect36_MtxStd_1 Matrix Standard Spect50_MtxStd_POP-7 ™ Sequencing Spect36_SeqStd_1 Sequence Standard Spect50_SeqStd Note: Do not change settings in the Edit Parameters dialog box. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 100: Create A Spectral Calibration Plate

    4. In row A01, enter a sample name, click the next cell, then enter a comment if needed. 5. In column 1 of row A01, select the instrument protocol you created for the spectral calibration. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 101: Run The Spectral Calibration Plate

    2. In the Add Plate field, type the Plate ID then press Enter or scan the bar code of a plate to add it to the input stack. 3. Place the plate assembly into the instrument. See “Place plate assemblies into the instrument“ on page 44. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 102: Place Plate Assemblies Into The Instrument

    5. When the run is finished, remove the plate from the instrument. Place plate 1. Open the stacker drawer. assemblies into 2. Open the door of the In Stack tower. the instrument 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 103: View The Pass/Fail Status After The Run

    Event Log. status after the 1. In the navigation pane of the Data Collection Software, select Instruments4 ga37304 Instrument name4 Instrument Status4 Event Log. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...
  • Page 104 Quality (Q) value and condition number passing ranges Default Condition Number Dye Set Quality Value minimum Range Z_BigDyeV3 >0.93 2.5–4.5 E_BigDyeV1 3.0–5 >0.95 8.5–13.5 G5-RCT 5.0–8.0 >0.8 Any4Dye 1.0–20.0 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 105: Evaluate The Spectral Calibration Data

    Instruments4 ga37304 Instrument name4 Spectral Viewer. Spectral profile Raw data (matrix standards) Rename or set the active calibration 2. In the Dye Set drop-down list, select the dye set. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...
  • Page 106 See also“Magnify the spatial profile“ on page 96. Attribute Acceptance criteria Example Order of the peaks in 4-dye: blue-green- the spectral profile yellow-red (intensity vs pixel) from left to right 5-dye: blue-green- yellow-red-orange 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...
  • Page 107 Extraneous peaks in None the raw data profile Note: The E5 profile (intensity vs scan) may include extraneous peaks outside the matrix peak region, which can be ignored. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 108: Magnify The Spectral Profile

    (a feature that reduces signal variation between dyes of different fluorescent efficiencies). Magnify the 1. Click-drag the cursor to create a box around the area of interest. spectral profile 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...
  • Page 109 Chapter 8 Calibrate the instrument Perform spectral calibration for sequencing and fragment analysis 2. Release the mouse button. 3. Press R to reset the view. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 110: Examples Of Passing Sequencing Spectral Calibrations

    Perform spectral calibration for sequencing and fragment analysis Examples of Dye set Z created from a sequencing standard passing sequencing spectral calibrations Dye set E created from a sequencing standard 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 111: Example Of A Passing Fragment Analysis Spectral Calibration

    Instrument name4 Spectral Viewer. Failed calibrations are marked with an asterisk *. 2. Select the dye set of interest. 3. Click the List of Calibrations list in the lower right-pane. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...
  • Page 112 Perform spectral calibration for sequencing and fragment analysis 4. Select the spectral calibration you want to use for future runs. 5. Click Set to display the selected spectral calibration in the Active Calibration text box. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 113: Chapter 9 Maintenance

    Ensure the plate assemblies are properly positioned on the plate deck. Plates Before each run should sit snugly on the deck. IMPORTANT! Do not use warped plates. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 114: Weekly Maintenance

    Clean the buffer jar, water, waste, and buffer reservoirs with warm water, then Weekly rinse with distilled/deionized water. Flush the polymer delivery pump water trap. See “Flush the water seal trap“ on Weekly page 122. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 115: Monthly Maintenance

    Change the capillary array using the Install Array wizard (see “Typical conditions As needed for using maintenance wizards“ on page 117). Remove any dried polymer from the capillary tips. Use a lint-free wipe moistened As needed with deionized water. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 116: Wizards Types And Functions

    – To replace old polymer in the polymer delivery pump • To prepare the instrument for a shutdown >1 week Instrument Shutdown • To remove the capillary array 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 117: Typical Conditions For Using Maintenance Wizards

    Install Array wizard. The instrument will Use the Instrument Shutdown wizard. — be idle for >1 week. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 118: Effects Of The Instrument Door State On Wizard Tasks

    When you use Manual Control to move the autosampler, watch all autosampler content movements and positions. If the autosampler reaches its maximum travel position, a fatal instrument error is generated. The software must be re-started after this error. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 119: Clean The Instrument

    IMPORTANT! When this wizard runs, it introduces polymer into the anode buffer jar. Replace the anode jar buffer after you run this wizard. See “Fill the anode buffer jar“ on page 33. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 120: Change Polymer Type

    IMPORTANT! When this wizard runs, it introduces polymer into the anode buffer jar. Replace the anode jar buffer after you run this wizard. See “Fill the anode buffer jar“ on page 33. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 121: Clean The Polymer Delivery Pump (Pdp) And Lower Polymer Block

    • Clean the exterior 1 time per week, when you replenish polymer. • Flush the polymer delivery pump water trap 1 time per week. • Clean the polymer delivery pump chamber, channels, and tubing 1 time per month. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 122: Clean The Polymer Delivery Pump Chamber, Channels, And Tubing

    3. Open the Luer fitting by grasping the body of the fitting and turning it and the attached syringe approximately one-half turn counterclockwise. 4. Open the exit fitting at the top left side of the pump block by turning it approximately one-half turn counterclockwise. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 123: Install The Capillary Array

    Replace the anode jar buffer after you run this wizard. See “Fill the anode buffer jar“ on page 33. 4. Perform a spatial calibration (see “Perform spatial calibration“ on page 91). 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 124: Store A Capillary Array

    5. Attach the detection cell with cover to the storage post on the capillary array frame. Header shipping cover Buffer reservoir Detection cell with cover Storage post on the capillary array frame Vial with the array tip 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 125: Clean The Detection Cell

    3. Turn the capillary array knob 1/4-turn clockwise. The pointer on the knob is pointed to the left. 4. Pull the pump and lower polymer blocks forward until the detection cell is out of the detection block. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 126: Perform A Short-Term Shutdown

    Perform a short-term shutdown Perform the short-term shutdown procedure if the instrument will be idle for <1week. Materials required • 1X run buffer • Deionized water • Lab wipes • Gloves 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 127: Perform A Short-Term Shutdown

    4. Remove the buffer, water, and waste reservoir assemblies from the instrument. 5. Disassemble each reservoir assembly and empty the contents of the reservoirs into an aqueous waste container. Aqueous waste 6. Rinse each reservoir using deionized water. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...
  • Page 128 2. Assemble the reservoir assembly 2. Assemble each reservoir as shown below. assembly as shown below. Retainer Retainer Septum Septum Reservoir cap Reservoir cap Reservoir Reservoir Plate base Heated plate base Power cable 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...
  • Page 129 Buffer reservoir Buffer position 12. Place the water and waste reservoirs in the instrument. Load the three reservoirs in the following order. a. Buffer reservoir 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 130: Maintain Adequate Space For Database And Sample Data Storage

    If sufficient disk space is not available to store the run data, the following occurs. • An error message is displayed in the Error Code pane in the Event Log. • The status bar displays a flashing 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 131: Full Database Error

    “Delete data from the database“ on page 132. Cleanup Processed Plates Check available Check disk space monthly. disk space on E: 1. In the navigation pane of the Data Collection Software, select drive Instruments4 Database Manager. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 132: Delete Data From The Database

    1. In the navigation pane of the Data Collection Software, click Instruments4 Database Manager. The Database Manager opens. 2. Click Cleanup Processed Plates. The Cleanup Database dialog box opens. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...
  • Page 133 Chapter 9 Maintenance Maintain adequate space for database and sample data storage 3. Click OK. Note: It may take several minutes to clean up the database. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 134: Restart The Instrument And The Computer

    5. Power on the instrument and wait until the green status light on the front panel is on and not flashing before proceeding. 6. Log in to Windows ™ operating system. 7. Start the Data Collection Software. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 135: Appendix A Troubleshooting

    3. Perform the required tasks to fix the problem. Review a run log file Run log files are stored in the default directory, not in the destination directory specified by the results group. Navigate to E:/AppliedBiosystems/UDC/DataCollection/Log. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 136: Hi-Di ™ Formamide Preparation And Storage

    2. Ensure that the Ethernet cable is connected to the back of the instrument and to the computer. 3. Display the Event Log and troubleshoot the error codes (see page 135). 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 137: Instrument Run Troubleshooting

    Unknown cause. Review the run log file. See “Review a run log file“ on page 135. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 138: Service Console Troubleshooting

    Clean the detection cell (see page 125). clean. Problem with capillary array. Use the Install Capillary Array wizard to replace the capillary array (see page “Store a capillary array“ on page 124). 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 139: Spectral Calibration Troubleshooting

    Bad dye order detected" in the spectral calibration protocol, then repeat the spectral calibration. instrument protocol. Problem with spectral Repeat the spectral calibration. calibration, even if dyes are in the correct order. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 140: General Troubleshooting

    The check valve is leaking. Remove the polymer bottle to expose the check during pump fill stroke valve. Push 100 mL of DI-water through the check valve with a 50‑cc syringe and adapter. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...
  • Page 141 “Prepare the plate assemblies“ on page 42. Plate sensor malfunction. Contact Support. "Full database" message Insufficient disk space. See “Maintain adequate space for database and sample data storage“ on page 130. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 142: Appendix B Instrument Specifications

    Instrument specifications Instrument specifications Table 9 3730xl DNA Analyzer physical dimensions, weight, and power consumption Component Height Length (depth) Width Weight Instrument 90 cm (35.5 in.) 65 cm (26.0 in.) 100 cm (40.0 in.) with ~186 kg (411 lbs) door closed 170 cm (67.0 in.) with...

  • Page 143: Environmental Requirements

    Typical environments with a Pollution Degree II rating are laboratories and sales and commercial areas. • 15–30°C (59–86°F) (Room temperature should not Operating conditions fluctuate ±2°C during an instrument run) • 20–80% relative humidity, noncondensing 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 144: Electrical Requirements

    If the supplied power fluctuates beyond the rated voltage, a power line regulator may be required. High or low voltages can adversely affect the electronic components of the instrument. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 145: Power And Communication Connections

    Power and communication connections Instrument Computer Monitor Keyboard Because of the Ethernet cable connection between the instrument and the computer, the computer must be located within 9 feet of the instrument. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 146: Appendix C Run Modules

    Extra long read XLRSeq50_POP7 ≥900 BDx_XLRSeq50_POP7 Long read LongSeq50_POP7 ≥850 BDx_LongSeq50_POP7 Fast read FastSeq50_POP7 ≥700 BDx_FastSeq50_POP7 Standard read StdSeq36_POP7 ≥700 BDx_StdSeq36_POP7 Rapid read RapidSeq36_POP7 ≥550 BDx_RapidSeq36_POP7 Short read TargetSeq36_ POP7 ≥400 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...
  • Page 147 Time stated for 400 bases. Module can be customized to run 200-400 bases. IMPORTANT! Use BDX run modules only if you prepare samples with BigDye ™ XTerminator Purification Kit. Use non-BDX run modules for samples purified with other methods. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 148: And Any5Dye

    When using this dye set, perform spectral calibrations each time the capillary array is replaced or moved within the detection cell 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 149: Dye Sets G5 And G5-Rct For Fragment Analysis

    • Fragment analysis with a 96-capillary array dye sets • Applications with a high dynamic range (where the signal intensity of large peaks is much higher than the signal intensity of small peaks) 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 150: Appendix E Catalog Numbers

    Plate Base (heat seal) for 3730/3730 4334877 xl systems, 384 well Plate Retainer (septa seal) for 3730/3730 4334868 Heat Seal Film for Sequencing and Fragment Analysis Sample Plates 4337570 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 151: Instrument Consumables

    3730/3730xl DNA Analyzer Sequencing Standards, BigDye ™ Terminator v1.1 4336799 Fragment analysis DS-33 Matrix Standard Kit (Dye Set G5) 4345833 DS-36 Matrix Standard Kit (Dye set J6, 6-dye) 4425042 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 152: Fragment Analysis Size Standards

    ™ Size Standard 4324287 GeneScan ™ 500 ROX ™ Size Standard 401734 GeneScan ™ 600 LIZ ™ Size Standard v2.0 4408399 GeneScan ™ 1200 LIZ ™ Size Standard 4379950 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 153: Appendix F Safety

    Safety symbols Symbol and description CAUTION! Risk of danger. Consult the manual for further safety information. CAUTION! Risk of electrical shock. CAUTION! Hot surface. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...
  • Page 154 MISE EN GARDE ! Risque de choc électrique. MISE EN GARDE ! Surface chaude. MISE EN GARDE ! Danger biologique potentiel. MISE EN GARDE ! Rayonnement ultraviolet. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 155: Location Of Safety Labels

    Figure 20 Safety symbols that are visible with the oven door closed Physical hazard Electric shock Figure 21 Safety symbols that are visible with the oven door open Laser 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 156: Control And Connection Symbols

    Both direct and alternating current Conformity Conformity mark Description symbols Indicates conformity with safety requirements for Canada and U.S.A. Indicates conformity with China RoHS requirements. Indicates conformity with European Union requirements. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 157: Safety Information For Instruments Not Manufactured By Thermo Fisher Scientific

    Physical injury CAUTION! Moving Parts. Moving parts can crush, pinch and cut. Keep hands clear of moving parts while operating the instrument. Disconnect power before servicing. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 158: Electrical Safety

    AVERTISSEMENT ! Déconnecter l’alimentation. Pour déconnecter entièrement l’alimentation, détacher ou débrancher le cordon d’alimentation. Placer l’instrument de manière à ce que le cordon d’alimentation soit accessible. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 159: Cleaning And Decontamination

    Follow local municipal waste component and ordinances for proper disposal provision and contact customer service for information accessory about responsible disposal options. disposal 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 160: Laser Safety

    Safety and electromagnetic compatibility (EMC) standards The instrument design and manufacture complies with the standards and requirements for safety and electromagnetic compatibility as noted in the following table: 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 161: Safety Standards

    AS/NZS CISPR 11 Limits and Methods of Measurement of Electromagnetic Disturbance Characteristics of Industrial, Scientific, and Medical (ISM) Radiofrequency Equipment Industrial, Scientific and Medical (ISM) Radio Frequency ICES-001, Issue 4 Generators 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 162: Environmental Design Standards

    SJ/T 11364-2014 “China RoHS” Standard—Marking for the Restricted Use of Hazardous Substances in Electronic and Electrical Products For instrument specific certificates, visit our customer resource page at www.thermofisher.com/us/en/home/technical-resources/rohs-certificates.html. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 163: Chemical Safety

    : lunettes de sûreté, gants ou vêtements de protection). · Limiter l’inhalation des produits chimiques. Ne pas laisser les récipients de produits chimiques ouverts. Ils ne doivent être utilisés qu’avec une ventilation adéquate (par exemple, sorbonne). 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 164: Biological Hazard Safety

    Biomedical Laboratories (BMBL), 5th Edition, HHS Publication No. (CDC) 21-1112, Revised December 2009; found at: www.cdc.gov/biosafety/publications/bmbl5/BMBL.pdf · World Health Organization, Laboratory Biosafety Manual, 3rd Edition, WHO/CDS/CSR/LYO/2004.11; found at: www.who.int/csr/resources/publications/biosafety/Biosafety7.pdf 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 165: Documentation And Support

    • Worldwide contact telephone numbers • Product support information – Product FAQs – Software, patches, and updates – Training for many applications and instruments • Order and web support 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 166: Limited Product Warranty

    Life Technologies Corporation and/or its affiliate(s) warrant their products as set forth in the Life Technologies' General Terms and Conditions of Sale at www.thermofisher.com/us/en/home/global/terms-and-conditions.html. If you have any questions, please contact Life Technologies at www.thermofisher.com/support. 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...

  • Page 167: Index

    165 front panel indicators 14 requirements, environmental 143 results, view, mobile device 50 run log file 135 green indicator light 14 GS600 LIZ v2.0 size standard 152 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...
  • Page 168 Thermo Fisher Connect, disconnect instrument 53 condition number 104 ThermoFisher Connect, analysis software 23 quality value 104 spectral calibration standard 99 support, customer and technical 165 symbols warranty 166 conformity 156 3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide...
  • Page 170 thermofisher.com/support | thermofisher.com/askaquestion thermofisher.com 1 October 2018...

Table of Contents