Orflo MOXI Z Quick Start Manual

Quick Start Guide
Before you Begin:
Your Moxi Z comes with a partial battery charge
and can be used immediately. Alternatively, you
can charge the Moxi by connecting it to a
computer or power adapter via the supplied USB
cable. Be sure to disconnect the USB cable prior
to running samples.
Required Materials:
- Moxi Z cassettes and diluent.
- Biological cell sample.
- Pipette and tips for 75μL aliquot.
Sample Prep Options:
-Type S Cassettes. Size range: 3 - 20um avg. dia.
Concentration range: 3,000 to 2,500,000 cells/ml
-Type M Cassettes. Size range: 4 - 25um avg. dia.
Concentration range: 3,000 - 500,000 cells/ml
For Adherent Cells: Aspirate media and detach
cells with appropriate disassociation media
(e.g., Accutase).
User Manual:
An electronic copy of the complete Moxi Z user
manual is on the USB ash drive that
was included in the Moxi Z kit.
MoxiChart Software:
An electronic copy of MoxiChart is on the
USB ash drive that was included in the Moxi Z kit.
MoxiChart facilitates histogram downloading and
rmware updates via Bluetooth and is compatible
with PC and MAC.
Mini Automated
Cell Counter
1) Turn Moxi ON 2) Depress Tray and
Insert Cassette
5) Data Summary
Dec 16 14:03:00
Type M
Cassette Type
500
Curve Fit
Cell Size Scale
0
Count (within
1 5
curve t or gated Curve Fit Count
1.28e+05 Cells/ml
area)
Press for
Done Gating
Gating Mode
Curve Fit Results
3) Load 75μL Sample
TEST: 01
MPI= .99
Touch
Gating
Touch
10 15 20 25 30
Diameter (um)
Average Size
9.218 um (dia)
1.55 pL (vol)
Curve Fit
2-26um Delete
4) Touch Screen to Start
Dec 16 14:03:00
System is Ready ...
Touch Screen When Loaded...
Touch Here
for
Small Particle Mode
Back
MPI - Moxi Population
Dec 16 14:03:00
TEST: 01
Index
MPI= .99
Type M
1.28e+05/mL
Touch to Move Gates
500
Adjust Count Scale
Population Histogram
Avg. Cell Size (dia. & vol.)
0
15 20 25
1 5 10 30
Diameter (um)
Gated Count Average Size
9.218 um (dia)
Press to
1.28e+05 Cells/ml
1.55 pL (vol)
Change Scale:
2-34, 2-26,
Back Curve Fit 2-26um Delete
2-18, 2-10μm
Gated Results
Touch Here
for Cells
< 8μm Dia.
Nov 2011 Rev 3.7
Made in the USA