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Orflo moxi z User Manual

Mini automated cell counter
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  • Page 2 This manual is believed to be complete and accurate at the time of publication. In no event shall Orflo Technologies or any of its affiliated corporations be liable for incidental or consequential damages in connection with or arising from the use of this manual.

  • Page 3: Table Of Contents

    Installing MoxiChart Software on a PC ................... 15 Connecting to Moxi Z via Bluetooth with a PC ................ 15 Connecting to Moxi Z via USB (requires Moxi Z OS v4.0 or greater) ........17 Installing MoxiChart Software on a Mac .................. 18 Connecting to Moxi Z via Bluetooth with a Mac ..............
  • Page 4 Specifications for the Moxi Z Automated Cell Counter ............... 26 Ordering Information ........................28 Technical Service ........................29 Warranty ............................. 29 Applicable Patents ........................30...

  • Page 5: Introduction

    Index (MPI) are displayed for each sample. Test results are also displayed in the form of a histogram. The Moxi Z unit can store approximately 500 histograms, and if desired, the data may be downloaded to a PC or Mac via a Bluetooth wireless connection or via USB connectivity (v 4.0 or greater).

  • Page 6: Safety Precautions

    Safety tested and certified by TÜV SÜD Product Service Division. Safety Precautions Please review and understand the safety instructions below before operating the Moxi Z Cell Counter. WARNINGS: To avoid danger of electric shock, do not install the instrument in an area with a high •...

  • Page 7: Consignes De Sécurité

    Utiliser uniquement les accessoires autorisés (adaptateurs de courant universels, • câble USB).Si l’instrument est échappé et brisé, débrancher le câble USB et communiquer avec Orflo Technologies. Ne pas tenter de désassembler l’instrument. Moxi Z Mini Automated Cell Counter User Manual Page 3...

  • Page 8: Moxi Z Mini Automated Cell Counter Kit

    Moxi Z Mini Automated Cell Counter Kit The Moxi Z Mini Automated Cell Counter kit includes the Moxi Z instrument, USB Cable, Power Adapter (US and EU versions only), Cassette Dispenser, Calibration Check Beads, USB Flash Drive with software/user guide and 25 Cassettes (2 tests per cassette).

  • Page 9: Moxi Z Mini Automated Cell Counter Accessories

    Moxi Z Mini Automated Cell Counter Accessories Cassette Dispenser USB Cable & Power Adapter USB Flash Drive with software Calibration Check Beads Electronic Calibration Cassette (sold separately) Moxi Z Mini Automated Cell Counter User Manual Page 5...

  • Page 10: Installation

    MPI. When not in use, the Moxi Z should be connected to a charging source so that the battery will maintain a continuous charge and be ready for immediate use. If a low battery warning appears on the display, stop using the instrument and charge the battery or operate the Moxi Z using an AC power source.

  • Page 11: Materials Required

    Using the Moxi Z Automated Cell Counter Settings Set the date and time by pressing the Settings icon on the main menu of the Moxi Z. Then use the arrow keys and follow the instructions displayed on the screen. Cell Counting 1.

  • Page 12: Managing The Data

    Press the Small Particle Mode < 8 µm button to initiate the test and run in this mode. 6. The Moxi Z will begin the test and the histogram count results will be complete in approximately 8 seconds (Type M cassette) or 15 seconds (Type S cassette).
  • Page 13 Moxi Z coincidence correction algorithm, but without the full curve-fitting technique, it does not achieve quite the same level of accuracy. As a result, Orflo recommends the use of the curve-fitting count mode over gating mode whenever possible in order to generate the “true”...
  • Page 14 (Type M: 500,000 cells/ml, Type S: 2,500,000 cells/ml) then a warning will be provided in the form of yellow background and red “High Concentration” text in the counts box (see arrows). Moxi Z Mini Automated Cell Counter User Manual Page 10...

  • Page 15: Retrieving And Deleting Data

    4. Use the arrows to the right of the histogram to change the vertical scale of the histogram. The vertical scale can also be changed by vertically swiping a finger on the display up (to increase) or down (to decrease). Moxi Z Mini Automated Cell Counter User Manual Page 11...
  • Page 16 7. Press the Back icon to close the test results and return to the Histogram viewer screen. Press the Close icon to return to the Home screen. (note: changes to histogram view will not be saved) Moxi Z Mini Automated Cell Counter User Manual Page 12...

  • Page 17: Moxi Z Performance Test Using Calibration Check Beads

    Moxi Z Performance Test Using Calibration Check Beads A 5 mL sample of Calibration Check Beads (Cat. No. MXA005) is included with each MOXI Z Automated Cell Counter. The calibration check beads consist of polymer microspheres of a known size in solution at a known concentration. The beads can be used to test the system as well as for practice and troubleshooting.

  • Page 18: Moxi Z Performance Test Using Electronic Calibration Cassette

    Technologies for assistance. Moxi Z Help 1. For help with operation of the Moxi Z, press the Help icon on the Home screen. Visual instructions for inserting a cassette will be presented. 2. Press the Next icon to view the next visual instruction that demonstrates how to pipette a sample into the cassette.

  • Page 19: Installing Moxichart Software On A Pc

    Installing MoxiChart Software on a PC 1. Insert Moxi Z flash drive into a USB port on your computer. 2. If prompted to run MoxiChart Install, click YES and follow the on screen instructions. Otherwise, open ORFLO DISK folder and double click on the file MoxiChart_Win_Install.exe.
  • Page 20 Moxi Z. 4. If a Moxi Z unit is not detected (above screen is not displayed), your Bluetooth radio most likely requires Bluetooth device pairing (Windows) and, on Windows XP systems, a Bluetooth serial port connection to be established.

  • Page 21: Connecting To Moxi Z Via Usb (Requires Moxi Z Os V4.0 Or Greater)

    (appear as a drive on) the computer following completion of this file export process. 5. Open the Moxi Z drive folder and copy the files to your computer (drag and drop or copy and paste) Moxi Z Mini Automated Cell Counter User Manual...

  • Page 22: Installing Moxichart Software On A Mac

    Connecting to Moxi Z via Bluetooth with a Mac The procedure for connecting Moxi Z via Bluetooth with a Mac is the same as that for a PC. See the section entitled “Connecting to Moxi Z via Bluetooth with a PC” on pages 15-16.
  • Page 23 With the unit powered off, quickly press the power/reset button four times. When successful, the white Moxi Z screen will display with text under the Moxi Z logo that indicates the system is in firmware update mode. If unsuccessful, turn the power off and retry.

  • Page 24: Updating Moxi Z Firmware Via Usb (Requires Moxi Z Os V4.0 Or Greater)

    AC power adaptor). Unplug the power adapter to expose the USB connector for the computer. Next, the user needs to put the Moxi Z into a firmware update mode. This can be done in either of the following two ways: With the unit powered off, quickly press the power/reset button four times.

  • Page 25: Moxi Apps

    Moxi Apps Moxi Z applications can be entered from touching the Apps icon on the home screen. Included applications in version 4.3 are: Clock • Programmable timer (with sound notification) • Restore – restores unit to factory settings • Loader – Puts unit is Firmware/OS loader mode •...

  • Page 26: Troubleshooting

    Cells too large Incomplete Test size range of the cassettes being used Highly/strongly Try breaking apart cells using pipette trituration and/or aggregated cells clogging protease treatment. filter and blocking flow Moxi Z Mini Automated Cell Counter User Manual Page 22...
  • Page 27 If problem persists, return instrument for service. Defaults loaded reset all settings to factory defaults False start Sensor malfunction Use new sensor. False stop Moxi Z Mini Automated Cell Counter User Manual Page 23...

  • Page 28: Maintenance And Storage

    Upgrade Moxi Z system firmware to v3.6 or later earlier Maintenance and Storage Storage Store the Moxi Z Cell Counter and Moxi Z Test Cassettes at room temperature in a dry environment. Avoid exposure to ultraviolet light as it may discolor and/or damage the instrument. Charging the Battery The Moxi Z Automated Cell Counter contains a 3.7 V lithium ion battery which can be charged...
  • Page 29 The external surfaces of the Moxi Z Cell Counter body and touch screen display can be sanitized by wiping with a soft, nonabrasive cloth moistened with 70% isopropyl alcohol (IPA) or 70% ethanol. Do not clean the instrument with any more aggressive solutions.

  • Page 30: Specifications For The Moxi Z Automated Cell Counter

    Specifications for the Moxi Z Automated Cell Counter Performance Sample Volume Required 75 µL Dynamic Range 2 - 34 µm Average Cell Size 4 - 25 µm diameter (Type M cassette) 3 - 20 µm diameter (Type S cassette) Average Cell Volume...
  • Page 31 Moxi Z Cassette Specifications (Type M and Type S) Material Polyester Aspiration Volume 75 µL Dimensions Length: 3.3 in. (8.4 cm) Width: 1.2 in. (3.0 cm) Height: 0.035 in. (0.90 mm) Weight 0.1 oz. (2.3 g) Moxi Z Mini Automated Cell Counter User Manual...

  • Page 32: Ordering Information

    Ordering Information This section lists catalog numbers for the Moxi Z Automated Cell Counter and related products. You can purchase Orflo products through a regional distributor or on-line at www.orflo.com. See the Technical Assistance section for information about contacting Orflo.

  • Page 33: Technical Service

    In the event of a breach of the foregoing warranty, Orflo’s sole obligation shall be to repair or replace, at its option, the applicable product or part thereof, provided the customer notifies Orflo promptly of any such breach.

  • Page 34: Applicable Patents

    Applicable Patents The following core patents apply to the Moxi Z and cassettes. This is not intended to be an all- encompassing list of related patents. U.S. Patent # 7,520,164 • U.S. Patent # 8,171,778 • U.S. Patent # 8,182,635 •...

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