Do not allow unauthorized and/or untrained operative to use this device. Any misuse will be the sole responsibility of the user/owner and Sutter Instrument Company assumes no implied or inferred liability for direct or consequential damages from this instrument if it is operated or used in any way other than for which it is designed.
NEVER ALLOW THE FREE FLOW OF AIR TO BE RESTRICTED. NEVER ALLOW THE FREE FLOW OF AIR TO BE RESTRICTED. NEVER ALLOW THE FREE FLOW OF AIR TO BE RESTRICTED. NEVER ALLOW THE FREE FLOW OF AIR TO BE RESTRICTED. LAMBDA 10-2 OPERATION MANUAL – REV. 2.04 (20120224)
2.7.2 Use of Blanking Discs ......................18 2.7.3 Neutral Density Filters ......................19 3. 3. 3. 3. OPERATIONS OPERATIONS ....................................................21 OPERATIONS OPERATIONS ....................................................3.1 First Time Use ..........................21 LAMBDA 10-2 OPERATION MANUAL – REV. 2.04 (20120224)
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4.2.2.2 Special Commands: ON LINE, BATCH, and SHUTTER Commands......34 4.2.2.3 Output Command Structure: BUSY and ERROR lines..........36 4.2.3 Strategies for Controlling the Lambda 10-2 via the Parallel Port........37 4.2.3.1 Using Input Lines Only ....................37 4.2.3.2 Using Fewer than Eight Input Lines................37 4.2.3.3 Using the BUSY Line......................37...
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Table E-2. Filter command structure....................62 TABLE OF LISTINGS Listing 4-1. Batch transfer via PC parallel port program..............36 Listing 4-2. Program to obtain the Lambda 10-2 status on the PC parallel port......41 LAMBDA 10-2 OPERATION MANUAL – REV. 2.04 (20120224)
Introduction Introduction The Lambda 10-2 is an optical filter wheel system designed for rapid change of wavelength and comprises two subsystems: A controller and one or two filter wheels, each with or without a shutter, that are to be placed in the optical pathway of your existing experimental setup.
When a Lambda 10-2 Filter Wheel system is purchased, its configuration may include one to two filter wheels, each one with or without a shutter. If the Lambda 10-2 system is purchased with only one 10-position 25mm (1-inch) filter wheel, you may wish to add a second filter wheel of the same type later, in which case the second filter wheel can be added without modification.
7. Mounting ears 8. Optical port 9. Loading port (plug installed) 10. Shutter housing All filter wheel options are listed in the Sutter Instrument catalogue and in the following list. 10- - - - position, 25mm filter wheels: position, 25mm filter wheels:...
Filters Filters Filters The Lambda 10-2 filter changer is designed for use with any 1-inch or 25-mm filter with a thickness that does not exceed the following dimensions: 4.5 mm (0.18 in.) for Slide 4.5 mm (0.18 in.) for Slide 4.5 mm (0.18 in.) for Slide...
Sutter Instrument. only use shutters installed by Sutter Instrume nt. This upgrade is available only at Sutter Instrument’s factory (or through their distributors) and is listed as “SHUTTER” in the Sutter catalogue (or “WHEEL” in older catalogues). 1.2.6 Adapters 1.2.6...
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For larger rotations, the shaft will stop at the nearest of the 50 correct positions for that command signal when the external force is removed. LAMBDA 10-2 OPERATION MANUAL – REV. 2.04 (20120224)
The second individual filter holder and one position of the wheel (position #1) contain only retainers and spacers. The Lambda 10-2 is shipped to you in a prefabricated foam mold. Please take note of this method of packaging. Should it ever be necessary to ship the puller to another location, the same method of packaging should be employed.
At a minimum, we recommend that the Lambda 10-2 should be firmly mounted to a solid surface by the support stand, which is included with each unit. Do not depend on the optical coupling between the microscope and the Lambda 10 to support and stabilize the Lambda 10.
OFF. Figure 2-2. Lambda 10-2 Cabinet (front view) Plug the power cord provided with the Lambda 10-2 into the Line Input socket on the Power Entry Module and then to a power source of the appropriate voltage and frequency.
(Figure 2-4). If you need to purchase an adapter or require a custom adapter for your experimental setup, please contact the Technical Support staff at Sutter Instrument Company by phone (415 883-0128) or by E- mail (info@sutter.com).
3. Connect the controller and filter wheel(s) with the appropriate cable(s) provided. It is important that the screws anchoring the cable ends be tight to ensure a good connection for the motor power leads and positioning signals. LAMBDA 10-2 OPERATION MANUAL – REV. 2.04 (20120224)
B-OFF Figure 2-7. Initialization screen. 2. The Lambda 10-2 will initially be set to respond to the parallel port as its input source. To manually select a filter you must first press the LOCAL key on the keypad. The display will now indicate that the MANUAL (keypad) input source is active.
At each filter position on the filter wheel, is a filter cup assembly to hold one 1” filter. The Lambda 10-2 also comes with two removable 1” filter holders that can be placed into the optical pathway to accommodate “fixed” filters, such as neutral density filters. The filter holders will be either a “drop in”...
It is intended that the two slots be used for the removal and installation of the retaining ring when using the spanner wrench that is provided with the Lambda 10-2 (shown in Figure 2-9). Note how the design of the spanner wrench differs between 25, 32, and 50 mm filter sizes.
This is important because if you forcefully push on the filter holder while you are trying to load a filter, you run the risk of scratching the filter if the tool slips out of place! LAMBDA 10-2 OPERATION MANUAL – REV. 2.04 (20120224)
Loading filters into the filter cups on the filter wheel follows the same procedure as described above for loading filters into filter holders. There is, however, the additional complication of working through the filter wheel housing. To gain access to the filter cups on LAMBDA 10-2 OPERATION MANUAL – REV. 2.04 (20120224)
After identifying and positioning the filter cup to be loaded, it is advisable to turn off the controller before removing the retaining ring from the filter cup. Follow the procedures described above for installation of filters into filter holders. LAMBDA 10-2 OPERATION MANUAL – REV. 2.04 (20120224)
2.7.3 Neutral Density Filters 2.7.3 2.7.3 Neutral Density Filters Neutral Density Filters Neutral Density Filters The individual filter holders are typically used for neutral density filters and are installed as described above. LAMBDA 10-2 OPERATION MANUAL – REV. 2.04 (20120224)
Rear Panel Connectors Rear Panel Connectors All electrical connections are all made on the rear panel of the Lambda 10-2. See the FIRST TIME USE chapter for details on connecting the power cord and filter wheel cable(s). LAMBDA 10-2 OPERATION MANUAL – REV. 2.04 (20120224)
Figure 3-2. Lambda 10-2 Controller Keypad There are sixteen keys on the Lambda 10-2 keypad: Ten numerical keys (0 to 9), and six function keys. The microprocessor that controls the Lambda 10-2 reads the keys while they are depressed but the appropriate action will occur only when the key is released. If a key is pressed while a move is in progress but released after the move is over, the key will read normally.
“ON LINE” commands. ON LINE: ON LINE: This key transfers control of the Lambda 10-2 to an interfaced external source, ON LINE: ON LINE: such as a computer.
F1 = Wheel A or or or or F2 = Wheel B The selected filter wheel (A or B) will be indicated in the lower left corner of the controller display after the word “MANUAL”. LAMBDA 10-2 OPERATION MANUAL – REV. 2.04 (20120224)
The highest speed that can be used will depend on the weight of the filters mounted in the wheel. In a 25mm, 10-position filter wheel equipped with two typical LAMBDA 10-2 OPERATION MANUAL – REV. 2.04 (20120224)
Error Detection and Recovery Error Detection and Recovery The Lambda 10-2 contains two sensor systems that are used to monitor the actual position of the filter wheel. After the controller sends the step sequence for a move to a new filter position, it compares the output of these sensors with the expected position.
WHY NOT JUST USE A LARGER MOTOR. As the inertia increases, an increase in the force required to achieve a given acceleration and deceleration becomes necessary. Since the step motor in the LAMBDA 10-2 is operated at rated power, an increase in force could only be obtained by using a larger motor.
General General The Lambda 10-2 microprocessor sets the PARALLEL port, by default, as the input source upon startup and anytime the ON LINE key is pressed. An appropriate command from the remote computer through the PARALLEL port will also set that port as the active input source and the display on the controller will reflect that change on its bottom line.
Input Commands to the controller via the parallel port are structured in a one-byte (8-bit) signal. These eight bits are transmitted to the Lambda 10-2 controller via the eight input lines (Pin 2 through Pin 9) of the parallel port connector. The eight bits can be used to convey two types of input commands.
Binary Group Value Binary Group Value Binary Group Value Binary Group Value 0111 Examples Examples Examples Examples Parallel Port Pin # Parallel Port Pin # Parallel Port Pin # Parallel Port Pin # LAMBDA 10-2 OPERATION MANUAL – REV. 2.04 (20120224)
Lambda 10-2 controller’s serial or parallel port. When the controller is in the ON LINE (Remote) mode, the eight input lines on the parallel port are examined at 50 microsecond intervals.
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Sending the commands in the correct order assures the best performance. Microsoft QuickBasic The following listing lists a program for batch transfer through a PC parallel (printer) port. LAMBDA 10-2 OPERATION MANUAL – REV. 2.04 (20120224)
BUSY BUSY BUSY line will stay high until the correct filter is in position, but the ERROR ERROR ERROR ERROR signal will stay high only until Filter 0 (zero) is in position. LAMBDA 10-2 OPERATION MANUAL – REV. 2.04 (20120224)
Obviously, it is also possible for the host computer to control the timing of events based on the expected behavior of the Lambda 10-2 (as described above) using the BUSY BUSY line only to verify that the controller is in the expected state.
The details of the input and output signals were described in previous sections. Now we will discuss the way in which these signals may be used to control the Lambda 10-2 from a PC computer's parallel printer port. This information is provided as a limited example and is only intended to show the elements useful in controlling the Lambda 10-2 through a generic printer port.
The connections to the parallel port are made through a DB 25 connector on your computer. The pin assignments for the parallel port socket are (as viewed from the plug side): Figure 4-2. PC parallel (printer) port Table 4-4. PC and Lambda 10-2 parallel port pin assignments Pin # Pin #...
BUSY, although the polarity of this signal may differ from the corresponding printer BUSY signal. With the Lambda 10-2 BUSY line connected to Pin 11 of the computer’s parallel printer port, the input port can be read to obtain the status of the Lambda10-2. Using Microsoft QuickBasic, this would be done as follows: LAMBDA 10-2 OPERATION MANUAL –...
Listing 4-2. Program to obtain the Lambda 10-2 status on the PC parallel port. BUSY: OUT BASEPORT+2, 16 ‘Required on some computers ‘before data can be read FOR U = 0 TO 5 ‘Delay to allow Lambda 10-2 NEXT U ‘to set busy flag...
The command code structures, however, are quite similar. The connection and Command Structure required to control the Lambda 10-2 via the Serial port are discussed in this chapter.
Connecting to the Serial Port Connecting to the Serial Port The serial interface connector on the back of the Lambda 10-2 controller cabinet is a DB-9 connector. The connections are arranged so a standard DB-9 serial cable can be used to connect the controller to a PC serial port.
This byte is the raw datum that serves as a Filter Command to select the Filter Position, Switching Speed and Filter Wheel on the Lambda 10-2 controller. Typically, this binary value is sent as its decimal equivalent as described below.
Output Command Structure: Command Echo and <CR> Output Command Structure: Command Echo and <CR> With the serial interface, the LAMBDA 10-2 controller responds to each command by echoing the command back to the host computer. This indicates that the command was received and will be executed promptly.
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A similar subroutine can be used to hold the computer in a loop until the carriage return (13) is received. BUSYCHECK: IF LOC(1) > 0 THEN SerialInput$ = INPUT$(1, #1) ASC(SerialInput$)=13 THEN RETURN GOTO BUSYCHECK LAMBDA 10-2 OPERATION MANUAL – REV. 2.04 (20120224)
MAINTENANCE MAINTENANCE Routine cleaning of the Lambda 10-2 system is required to prevent excessive dust accumulations. This is done by wiping all exterior surfaces with a dry, soft, cotton cloth. All retaining rings should be inspected occasionally to be certain that they are seated into the filter holders and cups.
LIMITED WARRANTY LIMITED WARRANTY Sutter Instrument Company, a division of Sutter Instrument Corporation, limits the warranty on this instrument to repair and replacement of defective components for two years from date of shipment, provided the instrument has been operated in accordance with the instructions outlined in this manual.
Liquid light guide (2 meters, 3mm diameter). Includes C-mount, lens, and lens tube. Slide-in filter holders are for filter wheels equipped with a shutter. Drop-in filter holders are for filter wheels that are not equipped with a shutter. LAMBDA 10-2 OPERATION MANUAL – REV. 2.04 (20120224)
DSUB 9-pin male to DIN serial cable for connecting a Macintosh (pre- Mac OS X Apple Macintosh systems only) to the serial RS-232 port. CLAM10 CLAM10 CLAM10 CLAM10 25 pin breakout to BNC cable. LAMBDA 10-2 OPERATION MANUAL – REV. 2.04 (20120224)
Replace the active fuse with the spare and re-install the fuse holder and power cord. If the controller fails to power up with the new fuse installed, call Sutter Instrument technical support personnel for assistance. Figure 5-2. Fuse holder.
Power cord Power cord 10A, 250V, with safety ground plug 10A, 250V, with safety ground plug Power cord Power cord 10A, 250V, with safety ground plug 10A, 250V, with safety ground plug LAMBDA 10-2 OPERATION MANUAL – REV. 2.04 (20120224)
6.25 x 7 x 2 in (15.88 x 17.75 x 5.8 cm) Weight: Weight: Weight: Weight: 2 lb., 14 oz. (1.31 kg) 2 lb., 14 oz. (1.31 kg) 2 lb., 14 oz. (1.31 kg) 2 lb., 14 oz. (1.31 kg) LAMBDA 10-2 OPERATION MANUAL – REV. 2.04 (20120224)
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...........15 F1,F2..............23 holder ..............15 LOCAL..............23 loading............13, 15 numeric...............23 maximum thickness maximum thickness maximum thickness maximum thickness..........14 ON LINE............23 neutral density ...........19 SHUTR ...............23 retaining ring .............15 SPEED..............23 specifications ............4 LAMBDA 10-2 OPERATION MANUAL – REV. 2.04 (20120224)
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T T T T input commands ..........32 power technical specifications..........55 connection ............22 controller controller............55 controller controller consumption............55 dimensions dimensions dimensions dimensions ..........55, 56 voltage selector...........22 technical support .............1 power cord TTL lines ..............42 mains..............55 LAMBDA 10-2 OPERATION MANUAL – REV. 2.04 (20120224)
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..............55 unpacking ..............7 voltage selector switch..........9 V V V V W W W W vacuum fluorescent display........23 warranty ..............49 vibration ..............7 weight .............. 55, 56 weight weight weight NOTES NOTES NOTES NOTES LAMBDA 10-2 OPERATION MANUAL – REV. 2.04 (20120224)
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