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Summary of Contents for opti-sciences CCM-300
- Page 1 CCM-300 Chlorophyll Content meter for small leaves and difficult samples Operation manual 603–883-4400 www.optisci.com...
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Page 2: Table Of Contents
GFPIII operation manual Opti-Sciences, Inc. Introduction..............................3 Items included ..............................3 Initial Setup ..............................3 User interface: ..............................5 Opening screen:............................5 Measurement overview: ..........................6 Research overview: ............................8 The variables: .............................8 Fluorescence emission response to chlorophyll content: ...............8 The Kautsky induction effect .........................9 Re-absorption of the emitted chlorophyll fluorescence by the leaf chlorophylls: .......10 Temperature: ............................10... -
Page 3: Introduction
Opti-Sciences, Inc. Introduction The CCM-300 Chlorophyll Content Meter is a modulated fluorometer that uses the emission ratio of red chlorophyll fluorescence at 700nm to the far red emission fluorescence value at 735nm., utilizing a fiber optic probe. The source and detector wavelengths were selected from the Science developed by Gitelson A. - Page 4 GFPIII operation manual Opti-Sciences, Inc. Install 2 AA batteries as shown The fiber optic probe is attached to the unit with two threaded connections. Use caution when attaching the fiber, do not force the threads. The fiber should easily attach to the unit, and seat securely.
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Page 5: User Interface
GFPIII operation manual Opti-Sciences, Inc. User interface: The CCM-300 uses a touch panel for all user input. Green boxes typically indicate a control or an editable variable. Controls that are on the screen are usually rendered inactive if a popup window is active. -
Page 6: Measurement Overview
Furthermore, it is difficult to get reliable readings on very small leaf plants such as immature rice and wheat. For these reasons, Opti-Sciences decided that it was time to engineer an affordable solution for these difficult applications. This section provides an overview of this technology. - Page 7 In other words, this instrument is designed to work with all of the types of samples listed above, that are a problem for existing absorption systems. Opti-Sciences decided to use the research by Gitelson A. A., Buschmann C., Lichtenthaler H. K. (1999) “The Chlorophyll Fluorescence Ratio F735/F700 as an Accurate Measure of Chlorophyll Content in Plants”...
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Page 8: Research Overview
GFPIII operation manual Opti-Sciences, Inc. Research overview: Starting with Gitelson (1999): “ The ratio between chlorophyll fluorescence at 735 nm and the wavelength range 700nm to 710 nm, F735/F700 was found to be linearly proportional to the chlorophyll content (with determination coefficient, r , more than 0.95) and thus this ratio can be used as a precise... -
Page 9: The Kautsky Induction Effect
Bushman (2007). Measurements were made immediately after dark and light adaptation, and again every 15 seconds for 20 minutes. With the CCM-300 using the F735 nm / F700 nm ratio the Kautsky induction effect is negligible. Negligible Kautsky induction effects with the CCM-300 Unlike the CCM-300 F735 / F700 fluorescence ratio, other ratios such as F 690/ F735 have shown Kautsky induction effects. -
Page 10: Re-Absorption Of The Emitted Chlorophyll Fluorescence By The Leaf Chlorophylls
Furthermore, since longer excitation wavelengths reach deeper into the leaf, causing greater fluorescence re-absorption, the CCM-300 uses a 460nm diode for fluorescence excitation, and as a result, most fluorescence comes from mesophyll cells and re-emitted fluorescence is minimized (Gitelson 1999). - Page 11 GFPIII operation manual Opti-Sciences, Inc. Graph of variation due to fluorescence emission signal strength Each sample was measured 30 times at the same location. Samples with greater variation require an average of multiple measurements for the best results. For this reason, measuring options are included to provide averaging from 2 to 30 measurements, or averaging with outlier removal beyond 2 sigma, or median determination.
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Page 12: References
GFPIII operation manual Opti-Sciences, Inc. The CCM-300 with leaf clip Because chlorophyll content can be measured from a single side with this method, the opportunity to measure difficult samples now exists. This nondestructive method can be used to measure chlorophyll content of algae on rocks, of lichens, of cactus, of fruit, of seeds, and of moss. Because the... -
Page 13: Setup Mode
GFPIII operation manual Opti-Sciences, Inc. Setup Mode After assembling the instrument, go to the Setup mode to set the time and date stamp for all measurements. Use your finger or the stylus to tap the + or – boxes to set the date and time. -
Page 14: Measurement Mode
GFPIII operation manual Opti-Sciences, Inc. Measurement Mode Measure is for measuring samples. Go to the setup mode and adjust the time and date before using the measuring mode. In the measuring mode, a few parameters should to be setup before measuring begins. -
Page 15: For Measurements With The Leaf Clip
GFPIII operation manual Opti-Sciences, Inc. using five to thirty measurements. Median is the final option. Here, measurements from two to thirty can be selected and the Median is reported after the measurements are taken. For most applications, the instrument is now ready to make measurements. -
Page 16: For Measurements Without The Leaf Clip
GFPIII operation manual Opti-Sciences, Inc. The signal window on the bottom left corner can be very valuable. While it does not provide a chlorophyll content indication, it does indicate combined signal measuring strength. If the blue bar is in the green range a single measurement is probably adequate for measurement. -
Page 17: Data Management - File Utilities
GFPIII operation manual Opti-Sciences, Inc. Data Management – File Utilities Erasing a file on the instrument Data saved on the unit may be erased or viewed from this menu. Data may be moved between the unit and a host PC in the menu also. The unit will appear, on the pc, as a Removable Memory device if a USB... -
Page 18: Viewing A File On The Instrument
GFPIII operation manual Opti-Sciences, Inc. Tapping Erase a File will display a list of files that are on the machine. The Up/Down buttons will change color to indicate if any more data exists in that direction. Tapping on a file name in the list box will display a smaller window to confirm the erase action. -
Page 19: Diagnostics Menu
GFPIII operation manual Opti-Sciences, Inc. Diagnostics Menu Instrument operational values are shown here. This information can be useful for checking system functionality. In the event of trouble, the readings can be given to a service person. The top box shows current system values. The Signal reading is a measure of the fluorescence intensity detected. -
Page 20: Retrieving Data
Note: Do not disconnect the instrument without first using the eject function on the PC. Failure to use the eject function may result in the loss of data, and possible corruption of the CCM-300 file system. To use the eject function, select the device from Windows Explorer, right mouse click, and select eject. -
Page 21: Data Formats
GFPIII operation manual Opti-Sciences, Inc. Data formats: The format is stored in a comma separated variable format that can be easily imported into popular spreadsheet programs. A header in each file identifies the specific data field. Sample Test Format: Sample #, Date, Time, Raw Signal, CFR, Chlorophyll content in mg m 1,Oct/11/2011,11:49:43,751,0.33, 600... - Page 22 GFPIII operation manual Opti-Sciences, Inc. • PC interface: Unit appears as USB storage device to host PC • Power Supply: 2 AA batteries. Can use either NiMH or alkaline batteries. A charger is supplied. • Operating Life: Typically 8 to 10 hrs with freshly charged batteries •...
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