opti-sciences ccm-200 plus Operator's Manual
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opti-sciences ccm-200 plus Operator's Manual

Chlorophyll content meter
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CCM-200 plus
Chlorophyll Content Meter

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Summary of Contents for opti-sciences ccm-200 plus

  • Page 1 CCM-200 plus Chlorophyll Content Meter...

  • Page 2: Table Of Contents

    Opti-Sciences, Inc. CCM-200 plus operator's manual Table of contents TABLE OF CONTENTSINTRODUCTION ......................2 INTRODUCTION ...............................3 WELCOME! ................................3 ..............................3 IST OF QUIPMENT ............................3 BOUT THE NSTRUMENT & F ............................4 PPLICATIONS EATURES Applications............................4 Features..............................4 How it Works............................5 ..............................6 HYSICAL EATURES OPERATION OVERVIEW ............................6...

  • Page 3: Introduction

    CCM-200 plus operator's manual Introduction The CCM-200 plus is easy to operate. Contained herein are a few simple guidelines to assist the user. Throughout this manual, you will be shown setup options and response messages. When an example of a program screen is shown within a box, this is how it will appear on the CCM-200 plus.

  • Page 4: Applications & Features

    CCM-200 plus operator's manual Applications & Features The CCM-200 plus Chlorophyll Content Meter is a versatile tool for Crop, Plant and Agronomic Sciences. Listed below are a few examples of the many applications for which the CCM-200 plus can be used along with the user-friendly features that make the instrument indispensable to the field researcher.

  • Page 5: How It Works

    Figure 1 chlorophyll absorbance CCI = %Transmittance at 931nm. / %Transmittance at 653nm. The CCM-200 plus uses transmittance to estimate the chlorophyll content in leaf tissue. Two wavelengths are used for absorbance determinations. One wavelength falls within the chlorophyll absorbance range while the other serves to compensate for mechanical differences such as tissue thickness (see figure 2).

  • Page 6: Physical Features

    GPS port Operation Overview The CCM-200 plus is a self-contained, handheld device powered by a 9V alkaline battery. The sample head is located at the top of the instrument. A liquid crystal display (LCD) is located near the center of the unit. The power switch is at the bottom of the front panel. Seven menu / control keys are used to access all program functions.

  • Page 7: System Settings

    Described on the following pages are the specifics on how to obtain measurements with the CCM-200 plus as well as the overall instrument operation protocols. Calibration, measurements, memory and data storage management and t he five operating modes and each key function are...

  • Page 8: Measurements

    Opti-Sciences, Inc. CCM-200 plus operator's manual Measurements Turn on the instrument. After a few seconds, the main menu screen appears. Press the Run button. Before measuring, use setup to select the correct parameters and test type. Setup Use the arrow keys to scroll through the options.

  • Page 9: Calibration

    Opti-Sciences, Inc. CCM-200 plus operator's manual to scroll through numbers 0-9, and A-Z. To move to the next or previous characters, use the right and left arrows. Press accept to save the entry. The newly created file name is now set to be used for measurements.

  • Page 10: Test Types

    Test Types There are three test types on a standard CCM-200 plus. Single point measurement This test saves every data point. Place the sample in the chamber, and press the head closed. A value will appear when the test is complete.
  • Page 11 Opti-Sciences, Inc. CCM-200 plus operator's manual Note: If the Multi-point std dev option is chosen then the system will only allow averaging of between 10 and 30 points. Press Exit go back to the test menu. Press measure to start taking measurements, or if needed, calibrate the unit.

  • Page 12: Enter Comments For Measurements

    Opti-Sciences, Inc. CCM-200 plus operator's manual Enter Comments for measurements If desired, a comment can be entered for each measurement. It may also be entered only when change is made, such as measuring location or sample type. The comment will only appear with the specific measurement.
  • Page 13 Opti-Sciences, Inc. CCM-200 plus operator's manual Graphic display of Comment entry May 1 1:58:51 Data System Test Menu Measure Back Setup Clear sample area and hold down arm to calibrate. Back !Warning! File already exists Press Overwr or Append Select next action.

  • Page 14: Data Management

    Sciences. A serial cable is not supplied, however it is available at no charge upon request. Connect the RS232 cable to the CCM-200 plus and the host PC. Enter the data management window by pressing the data button form the main menu.

  • Page 15: Communication Settings

    Opti-Sciences, Inc. CCM-200 plus operator's manual Open a terminal emulation program, such as Hyperterminal (included with most installations of Windows). Communication Settings Data is sent in an ASCII text format with carriage returns separating each point. The default host settings are 9600 baud, 8 bit, 1 stop bit, no parity, and no handshaking. This mode is ignored if no data has been saved to memory yet.

  • Page 16: Technical Specifications

    Opti-Sciences, Inc. CCM-200 plus operator's manual Technical Specifications Measured Parameters: Optical Transmittance at 653nm., and at 931nm. CCI = % Transmittance at 931nm. / % Transmittance at 653 nm. Measurement Area: 3/8” diameter circle, or 9.53 mm diameter circle area 0.11in , or 71.22 sq mm...

  • Page 17: Error Codes

    Opti-Sciences, Inc. CCM-200 plus operator's manual ERROR CODES The error codes describe the problem encountered. Error Codes miscellaneous message text info for version 1.3a 5/11 rr Boot up message: errors with uSD memory: "SD init error" "Continue" "SD error #xx"...
  • Page 18 Opti-Sciences, Inc. CCM-200 plus operator's manual Reconnect the CCM to the PC. Go to My Computer, find the CCM200 drive, and right click the drive. Left click Format, and then left click Start. This will reformat the drive. All data that was on the instrument will be lost.
  • Page 19 Opti-Sciences, Inc. CCM-200 plus operator's manual "Warning! Average must " "be>=10 pts for std dev" "mode. A setting of 10 " "points will be used." Fix: This message will appear for the standard deviation test mode when the number of samples is less than 10 points.
  • Page 20 Opti-Sciences, Inc. CCM-200 plus operator's manual "Light Leak!" Fix: This message indicates that there is too much light present during the test zero phase. This may be due to worn gaskets or non-flat samples. Return to Dealer for repair. Calibration errors: "Clean Sample Chamber!"...
  • Page 21 Opti-Sciences, Inc. CCM-200 plus operator's manual Data view & output errors: "File is Corrupt!" Fix: This error may occur due to a file being corrupted by an aborted transfer to the PC or by removing the unit’s battery instead of using the power switch to turn the unit off.

  • Page 22: Technical Support

    CCM-200 plus operator's manual Technical Support Opti-Sciences provides technical support for all products, either directly to end users or through our distributors. Please contact your local representative, or contact Opti-Sciences directly for questions including technical assistance, instrument maintenance and repair. Warranty service period is determined by instrument serial number.

  • Page 23: References

    CCM-200 plus operator's manual References: Knighton N. Bugbee B. A comparison of Opti-Sciences CCM-200 Chlorophyll Meter and the Minolta SPAD 502 Chlorophyll Meter Crop Physiology Dept, Utah State University CATE T. M. , and PERKINS T. D. (2003) Chlorophyll content monitoring in sugar maple (Acer saccharum) Tree Physiology 23, 1077–1079©...
  • Page 24 Opti-Sciences, Inc. CCM-200 plus operator's manual Morris J (2008) Macrotyloma axillare and M. uniflorum : descriptor analysis, anthocyanin indexes, and potential uses Genetic Resources and Crop Evolution Volume 55, Number 1 / February, 2008 Pg 5-8 Morris J., Wang M., Patrick D. Biber P. D.,(2007) Evaluating a Chlorophyll Content Meter on Three Coastal Wetland Plant Species.

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