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Summary of Contents for Sartorius stedim Vivaspin Turbo 15
- Page 1 Technical data and operating instructions ® Vivaspin Turbo 15 For in vitro use only 85032-541-63...
- Page 2 ® Vivaspin Turbo 15 – Introduction Storage conditions |shelf life Equipment Required ® Vivaspin Turbo 15 ultrafiltration spin columns should be stored at room For use with centrifuge temperature. The devices should be 1. Centrifuge with swing bucket or fixed used before the expiry date printed on angle rotor (minimum 25°).
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Page 3: Equipment Required
Equipment Required ® Equipment required Vivaspin Turbo 15 Centrifuge Rotor type Swing bucket Fixed angle Minimum rotor angle – 25° Rotor cavity Conical bottom tubes Rotor compatibility ® Please note: Vivaspin Turbo 15 (30 mm + 118 mm) is designed to fit into rotors that can accommodate Falcon 50 ml conical bottom tubes, e.g. -
Page 4: Operation
Operation 1. Select the most appropriate membrane Desalting | Buffer Exchange cut-off for your sample. For maximum 1. Concentrate sample to desired level. recovery select a MWCO at least 50% smaller than the molecular size of the species of 2. Empty filtrate container. interest. -
Page 5: Technical Specifications
Technical Specifications Table 1: Technical Specifications ® Vivaspin Turbo 15 Dimensions Total Length 77 mm 30 mm OD flange) 118 in tube Width 27 mm Active membrane area 7.2 cm Hold up volume of membrane <10 μl Dead stop volume* in swing out 100 μl Dead stop volume in fixed angle (25°) -
Page 6: Usage Tips
Usage Tips 1. Flow Rate 3. Sterilisation of Polyethersulfone Filtration rate is affected by several parame- Membranes ters, including MWCO, porosity, sample con- Polyethersulfone membranes should not centration, viscosity, centrifugal force and be autoclaved as high temperatures will temperature. Expect significantly longer spin substantially increase membrane MWCO. -
Page 7: Performance Characteristics
Performance Characteristics ® Table 3: Performance Characteristics Vivaspin Turbo™ 15 Time to concentrate up to 20x [min.] at 20°C and solute recovery % Mode Centrifuge Centrifuge Rotor Swing 25° Fixed bucket angle Start volume 15 ml 11 ml Min. Rec. Min. -
Page 8: Chemical Compatibility
Chemical Compatibility Table 4: Chemical Compatibility (2hr contact time) Solutions Solutions Compatible pH range pH 1–14 Compatible pH range pH 1–14 Acetic Acid (25.0%) Lactic Acid (5.0%) Acetone (10.0%) Mercaptoethanol (10 mM) Acetonitrile (10.0%) Methanol (60%) Ammonium Hydroxide (5.0%) Nitric Acid (10.0%) Ammonium Sulphate (saturated) Phenol (1.0%) Benzene (100%) -
Page 9: Ordering Information
Ordering Information Cut off Pack size Prod. no. 3,000 MWCO VS15T91 3,000 MWCO VS15T92 5,000 MWCO VS15T11 5,000 MWCO VS15T12 10,000 MWCO VS15T01 10,000 MWCO VS15T02 30,000 MWCO VS15T21 30,000 MWCO VS15T22 50,000 MWCO VS15T31 50,000 MWCO VS15T32 100,000 MWCO VS15T41 100,000 MWCO VS15T42... -
Page 10: Other Products
Other Products Product Sample volume Mode Membranes available ® Vivaspin 100 μl–600 μl Centrifugal Polyethersulfone ® Vivaspin 0.4 ml–2 ml Centrifugal Polyethersulfone, Cellulose Triacetate, ® Hydrosart Centrisart 0.5 ml–2.5 ml Centrifugal Polyethersulfone, Cellulose Triacetate ® Vivaspin 1 ml–4 ml Centrifugal Polyethersulfone ®... - Page 12 Sartorius Stedim Biotech GmbH August-Spindler-Strasse 11 37079 Goettingen, Germany Phone +49.551.308.0 Fax +49.551.308.3289 www.sartorius-stedim.com Copyright by Sartorius Stedim Biotech GmbH, Goettingen, Germany. All rights reserved. No part of this publication may be reprinted or translated in any form or by any means without the prior written permission of Sartorius Stedim Biotech GmbH.
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