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Blue-Ray Biotech BluePette User Manual

Single and multichannel electronic pipettes
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BluePette
Single and Multichannel Electronic Pipettes
User Manual
Version: 1.0
Copyright © 2018 Blue-Ray Biotech Corp.
All Rights Reserved.

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  • Page 1 BluePette Single and Multichannel Electronic Pipettes User Manual Version: 1.0 Copyright © 2018 Blue-Ray Biotech Corp. All Rights Reserved.

  • Page 3: Table Of Contents

    Table of Contents Safety Precautions ..............1 1.1. Operation Safety Precautions ............. 1 1.2. Battery Safety ................1 General Description ..............3 2.1. Features .................... 3 Getting Started................4 3.1. Unpacking ..................4 3.2. Inserting the Battery ..............5 3.3.
  • Page 4 MIX Mode..................18 10. SE (Sequential Aspirating/Dispensing) Mode ....19 10.1. SA (Sequential Aspirating) Mode......... 1 9 10.2. SD (Sequential Dispensing) Mode ........21 11. Sterilization................22 11.1. Disassemble Lower Part ............23 11.2. Reassemble Lower Part ............24 12. Maintenance ................25 13.

  • Page 5: Safety Precautions

    Congratulations on your new BluePette electronic pipette. Before using it for the first time, please read this entire user guide carefully. To guarantee problem free, safe operation of BluePette electronic pipette, it is essential to observe the following points: 1.1. Operation Safety Precautions...
  • Page 6 2. Never use the pipette while using the DC-in jack to charge the battery. 3. Do not incinerate the Lithium battery or expose it to excessive heat. 4. Do not short-circuit, puncture, crush, disassemble, damage, deep-cycle, re-vitalize or modify the battery. 5.

  • Page 7: General Description

    2. General Description This BluePette electronic pipette features an accurate and efficient, as well as ergonomic and light-weight design. The pipette is designed to conduct various liquid handling tasks in the laboratory with a reduced risk of strain andRepetitive Stress Injury (RSI).

  • Page 8: Getting Started

    3. Getting Started 3.1. Unpacking Open the package and check whether it contains the following items:  Single/8-channel unit  User guide  Certificate of conformity  AC-DC power adaptor  Hanger and accessories  Pipette tips  1 battery charger set and 1 extra battery If there are any items missing, damaged, or not according to your order, please contact your distributor or sales representatives for replacement immediately.

  • Page 9: Inserting The Battery

    3.2. Inserting the Battery Remove the battery cover by pressing its latch and tilting it to the side (see Figure 1). Insert the battery (see Figure 2) with the metallic contacts facing downwards. Close the cover of the battery compartment. Figure 1: Open the battery cover.
  • Page 10 during the charging process. When the battery is fully charged, the blinking will stop and the pipette will beep once to remind the user. Figure 3: Using the internal charging circuit to charge the battery Note: 1. For safety concerns, the pipette shall not be used during the charging period.

  • Page 11: Start Pipetting

    By simply pressing the button, the pipette will start START working. The BluePette is ergonomically designed. Hold the pipette as shown in Figure 4. Use your index finger to press the button, and your START thumb to press the ejector.

  • Page 12: Hanger Installation

    Figure 5: Hanger installation 4. Overview This section presents an overview of the BluePette (see Figure 6), and points out the locations of its buttons. The LCD display panel is also shown below (see Figure 7).
  • Page 13 LCD Display Keypad Battery Cover Reset Button Tip Ejector Start Button Start Button Connecting Nut Bushing Lower Part Tip Fitting (Front View) (Side View) Figure 6: 1-channel pipette overview SYS System Setting AUTO Auto Pipetting MD Multiple Dispensing Count No./Prog No. MIX Mixing SE Sequential Aspiration/Dispensing Volume (μl)

  • Page 14: Keypad Function

    4.1. Keypad Function Button Symbol Function Description Confirm your choice Set/Enter Exit to previous mode/setting Exit/Esc Increase volume/ change parameter Decrease volume/ change Down parameter Piston automatically determines Reset RESET the home position Activate aspirating/dispensing Start START Replace button Eject the tips Ejector Tips On Efficient Operation:...

  • Page 15: Acoustic Signals

    4.2. Acoustic Signals The acoustic signals are a great help when you are familiarizing yourself with the operating procedure of the BluePette. They can also be switched off if required (see Section 6.3.) Acoustic Operation Sound Low-tone beep Aspirating Dispensin...

  • Page 16: Function Map

    5. Function Map The main menu of the pipette includes five function modes (SYS, AUTO, MD, MIX and SE). Each mode includes various function settings (see Figure 8). Function Map Functio n AUTO Mode Aspirating/ Dispensing Aspirating Dispensing Volume Number Speed Volume (COUNT)

  • Page 17: Sys (System) Mode

    6. SYS (System) Mode SYS mode is used to set up the function settings before pipetting. Once users select and store these settings, they will no longer need to set up the settings before every pipetting. There are 7 function settings (Aspirating Speed, Dispensing Speed, Acoustic Signals, Tip Ejection Alarm, Blow-out, Reverse Pipetting and Program Recall) in SYS mode.

  • Page 18: Dispensing Speed

    6.2. Dispensing Speed 1. The “Dispensing Speed” symbol should now be flashing. 2. Press «Up/Down» to change the aspirating speed ( Slowest, : Fastest). 3. Press «SET» to confirm the speed selection and move to the “Acoustic Signals” function setting. 6.3.

  • Page 19: Reverse Pipetting

    the blow-out function in manual pipettes. To avoid the leftover final droplet in tips, the blow-out setting is recommended in all protocols. The blow-out setting will not be performed in “Reverse Pipetting” setting. 1. The “Blow-out” symbol should now be flashing. 2.

  • Page 20: Setting Prog (Programs)

    the pipetting. will appear on the LCD to remind users to dispense the remaining liquid. 6.7. Setting PROG (Programs) The memory program includes 9 different storage locations (PROG 01 ~ 09). Your favorite operating modes with user-selected settings can be stored to these locations for future recalls.

  • Page 21: Auto Mode

    START the volume setting. 8. MD (Multiple Dispensing) Mode In MD mode, the BluePette performs repetitive dispensing of a selected volume. To compensate the possible mechanical gap, users “MUST” select Reverse Pipetting in System Mode and blow out the residual volume in the end of every multiple dispensing cycle.

  • Page 22: Mix Mode

    excessive volume is needed to ensure equal operating condition for each dispensing step. 1. Press «EXIT» to enter the mode selection. 2. Press «Up/Down» to make the symbol start flashing. 3. Press «SET» to confirm the selection. The default pipetting volume will start flashing.

  • Page 23: Se (Sequential Aspirating/Dispensing) Mode

    2. Press «Up/Down» repeatedly until the symbol starts flashing. 3. Press «SET» to confirm the selection. The symbol will then appear. 4. Use «Up/Down» to select the desired mixing count 5. Press «SET» to confirm the selection. The default pipetting volume will start flashing.
  • Page 24 3. Press «SET» to confirm the selection. 4. Use «Up/Down» until the symbol starts flashing. 5. Press «SET» to confirm the selection. 6. Under the symbol, “01” will appear. Press «Up/Down» to set the volume of the first aspiration. 7. Press «SET»...

  • Page 25: Sd (Sequential Dispensing) Mode

    3. Sequential Aspirating (SA) function will not perform Reverse Pipetting even it is selected in System (SYS) mode. 10.2. SD (Sequential Dispensing) Mode The BluePette performs repetitive dispenses of the selected volumes. When the first aspiration is completed, the pipette will sequentially dispense the aspiration volume.

  • Page 26: Sterilization

    Cylinder lock Don't use force to pull the lower portion before release the cylinder lock. Figure 9: Wrong operation of Lower Part disassembly 11. After the sequential dispensing cycle, the pipette will make two beeping sounds. Pressing the button will re-activate the START whole cycle until the user changes the function mode.

  • Page 27: Disassemble Lower Part

    11.1. Disassemble Lower Part Always remember press button before RESET disassemble the lower part of the pipette. 1. Press the button. Wait until you hear two beeps. RESET 2. Loosen the lower part of the pipette by rotating the connecting nut in the clockwise direction as shown in Figure 10.

  • Page 28: Reassemble Lower Part

    Cylinder lock Figure 12: Push the cylinder lock downward Figure 13: The lower part will come off until you hear a click sound. easily and automatically. 11.2. Reassemble Lower Part Always remember to press button after you disassemble RESET the lower part of the pipette. Follow these steps to reassemble lower part of the pipette: 1.

  • Page 29: Maintenance

    12. Maintenance The outside of the pipette may be wiped clean with 60% Isopropanol, 70% ethanol or a suitable detergent such as Trigene, and then wiped with a lint-free cloth. If the pipette is severely contaminated or if very corrosive chemicals are dispensed, the lower part of the pipette should be disassembled and rinsed.
  • Page 30 self-diagnosis program. This program will constantly monitor the accuracy of pipetting volume, battery status and auto-calibration function. Error (Err) messages will appear on the display if the pipette fails to perform the attempted action properly. In case any Error (Err) messages or faults occur, please refer to the solutions in the following table to clear the error messages or faults.
  • Page 31 Foreign objects block the Use MIX mode tip fitting’s channel and distilled water to wash away the foreign objects Piston blocks Lubricate piston Power on failure Bad battery contact Reinsert the battery Rusted battery contact Replace with new battery Dead battery Charge the battery Function Mode...

  • Page 32: Appendix A: Technical Specifications

    Appendix A: Technical Specifications Automatic Pipetting (AUTO), Multiple Dispensing (MD), Mixing (MIX), Operation Mode Sequential Aspirating/Dispensing (SE), System Setup (SYS) Memories 9 sets Aspirating/Dispensing 5 speeds Speed Auto-calibration Power Engine High precision stepping motor Power Saving Yes, after 10 minutes 100/120/220/24 Power Adaptor 0 V AC-DC 3.6 V...

  • Page 33: Appendix B : Tip Ejector Height Adjustment

    Appendix B : Tip Ejector Height Adjustment 1. Remove the lower part of pipette as described in Section 11.1. 2. Remove the flat-head screw and bushing as shown in Figure 14. 3. Use flat-head screwdriver or other tool to rotate the tip ejector height adjustment nut.

  • Page 34: Appendix C: Warranty

    Appendix C: Warranty The BluePette are covered by a warranty for one year against defects in materials and workmanship. This period begins from the date of purchase, and within this period all defective parts will be replaced. The warranty does not cover defects caused by excessive tear and wear or...

  • Page 35: Appendix D: Ce Declaration

    4F., No. 31, Sec. 2, Chang-An E. Rd., Zhong-Shan Dist., Taipei City 10456, Taiwan (R.O.C.) Declaration of Conformity Product Name: BluePette Single/8-channel Electronic Pipettes Model Names: B20-1, B200-1, B1000-1, B20-8, B200-8 All models comply with the following European standards: EMC: EN55011, Class A...
  • Page 40 www.blue-raybio.com 4F., NO.31, Sec. 2, Chang’an E. Rd., ZhongShan Dist., Taipei City 10456, Taiwan (R.O.C) P/N: 401-apbr00-10...

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