Related Manuals for Blue-Ray Biotech TurboCycler 3
Summary of Contents for Blue-Ray Biotech TurboCycler 3
- Page 1 TurboCycler 3 High-Throughput Triple-Block Gradient PCR Thermal Cycler TCTT-4810 Operation Manual Ver. 1.0 Copyright © 2024 Blue-Ray Biotech Corp. All Rights Reserved.
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Page 3: Table Of Contents
Table of Contents 1. Safety Precautions ...................... 1 1.1 Intended Use ........................1 1.2 Safety Label ........................1 1.3 General Instrument Safety ..................... 1 1.4 Electrical Safety ......................2 2. General Description ....................3 2.1 Features ........................... 3 2.2 Product Overview ......................4 3. - Page 4 Mastermix Preparation Wizard ................23 Copy Number Convertor ..................23 Incubator ......................... 23 Help .......................... 23 8. System Setup ......................24 Service Mode ......................24 Administration ....................... 24 Factory Mode ......................24 9. Maintenance ....................... 25 9.1 Cleaning the Unit ......................25 9.2 Cleaning the Heated Lid ....................
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Page 5: Safety Precautions
1. Safety Precautions 1. Safety Precautions Before using the TurboCycler 3 for the first time, please read this entire operation manual carefully. To guarantee problem free, safe operation of the TurboCycler 3, it is essential to observe the following section. -
Page 6: Electrical Safety
Electrical Safety ELECTRICAL SHOCK HAZARD. Severe electrical shock can result from operating the TurboCycler 3 without its instrument panels in place. Do not remove instrument panels. High- voltage contacts are exposed when instrument panels are removed from the instrument. 1.4.1 Fuses FIRE HAZARD. -
Page 7: General Description
2. General Description 2. General Description TurboCycler 3 stands as the most ultimate high-throughput PCR thermal cycler in its class. With the capacity to run three distinct PCR protocols while handling 3 x 48 samples at once, you'll experience unrivaled flexibility. It provides fast gradient optimization and advanced ramp rate control, perfectly tailored to meet your specific assay requirements. -
Page 8: Product Overview
TCTT-4810 Operation Manual 2.2 Product Overview 2.2.1 Top View Lid Handle Front Panel USB Port Air Vents Figure 1. Top view Table 1. Detailed description for top view Name Function 10.1” high-resolution color LCD with capacitive touch panel. It displays the Front Panel current status of the system and allows the user to operate the instrument. - Page 9 2. General Description 2.2.2 Top View of Block With Opened Lid Lid Heater Platen High Temperature Warning Label Sample Block Figure 2. Top view of block with opened lid Table 2. Detailed description for top view of block with opened lid Name Function During and after PCR operation, the lid heater platen and the sample block...
- Page 10 TCTT-4810 Operation Manual 2.2.3 Rear View Air Vents Product Label Power Cable Socket Power Switch Figure 3. Rear view Table 3. Detailed description for rear view Name Function Power Cable Socket Power cable socket and fuse compartment. Power Switch Power On/Off switch. Indicates the model’s name, serial number, power specification, and Product Label other important information.
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Page 11: Getting Started
3. Getting Started 3. Getting Started 3.1 Unpacking Open the TurboCycler 3 package and confirm that all items below are included: ➢ TurboCycler 3 unit x 1 ➢ Quick operation guide x 1 (with QR code for downloading Operation Manual) ➢... -
Page 12: Lid Opening/Closing
TCTT-4810 Operation Manual 3.3 Lid Opening/Closing To open the lid, pull the handle upward and lift the lid backward as shown in Figure 4 to the upright position. Figure 4. Opening the lid To close the lid, pull the lid downward and close the handle to the lock position as shown in Figure Figure 5. - Page 13 Note If TurboCycler 3 is started cold, approximately two to three minutes will be required for the lid heater platen to reach the setting temperature. If the low-profile reaction vessels and the 5 mm compression mat are used, an extra seven minutes will be required to allow the compression mat to be heated to the required 130 °C temperature.
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Page 14: Loading The Reaction Vessel
TCTT-4810 Operation Manual 3.5 Loading the Reaction Vessel For the optimal performance of the TurboCycler 3, the recommended sample volume for 0.2 ml reaction vessels is 5-100 μl, and the Tube Frame should be applied if using tubes or strips. For low-... - Page 15 3. Getting Started To prevent the deformation of the low profile PCR vessels, and to ensure even distribution of the pressure from the lid, when PCR tubes or strip tubes are used, please load extra empty tubes on the 4 corners of the sample block as shown in Figure 8. Always make sure that all the sample tubes/ strip tubes are distributed evenly on the sample block.
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Page 16: Main Screen
TCTT-4810 Operation Manual 3.6 Main Screen On the Main Screen, there are some information items indicating the status of the TurboCycler 3, it contains 8 main function icons. There are three selections of blocks below the 8 main function icons. Tap on the block number or the icon to select the block. Please refer to Figure 9 and Table 5 for a detailed description. -
Page 17: User Folder Management
4. User Folder Management 4. User Folder Management After selecting a block, tap on the User icon on the main screen to enter the User Folder management screen. A sample screen is shown in Figure 10 below. Lock Symbol Figure 10. User folder overview All Protocols are stored inside the user folders. -
Page 18: Editing A User Folder
It is recommended to format your flash drive in FAT or FAT32 file system prior to using it on TurboCycler 3) for the TurboCycler 3 to recognize it and create a required folder in it. Tap on the USB icon to open the specific folder (\\TPCR_Files) in the flash drive and use it as a User Folder. -
Page 19: Protocol Management
5. Protocol Management 5. Protocol Management After entering the selected User Folder, the protocol list will be displayed. A sample screen is shown as Figure 11 below. Users can utilize the function icons on the lower part of the screen to edit, create, delete, duplicate (save as new protocol), and open the protocols. - Page 20 TCTT-4810 Operation Manual Changing Temperature/Time/Ramp Rate Setting 5.1.1 To select the Step, tap on the step segment. The selected Step will be highlighted and the Step Temperature, Time and Ramp Rate will be displayed. Tap on and a spinning wheel will pop up as shown in Figure 13, allowing you to modify the Step Temperature, Step Time, or Step Ramp Rate.
- Page 21 5. Protocol Management Number. Spin the wheel to the desired number and tap on to accept the setting or tap on to abort the operation. The Stage Cycle Number range: 2 to 99. Figure 14. Cycle number setting Advanced Option Settings 5.1.4 Users can set up the following advanced option functions for the Steps in a multi-step Stage: 1) Temperature Gradient...
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Page 22: Saving A Protocol
TCTT-4810 Operation Manual 1. Tap on to accept the setting or tap on to abort the operation. A table will pop up displaying the calculated gradient temperature from row A to H. Tap on confirm the setting. Note The temperature values between the B and G rows are calculated values and represent an algorithmic based on the heat distribution throughout the sample block. -
Page 23: Editing (Viewing) A Protocol
5. Protocol Management 5.3 Editing (Viewing) a Protocol To edit a Protocol in the User's protocol list, tap on the Protocol name to select it first. The selected Protocol will be highlighted. You can then tap it the second time or tap on the Open icon open it. -
Page 24: Protocol Running Management
TCTT-4810 Operation Manual 6. Protocol Running Management Caution: During the running of the protocol, the lid heater and the sample block may become very hot. Please be aware of the high temperature. 6.1 Running a Protocol Please refer to Section 5.3 Editing (Viewing) a Protocol to open and check the setting of a Protocol. - Page 25 6. Protocol Running Management Figure 17. Sample block control mode Tap on the Next icon to go to the Multiple Blocks selection mode screen. Multiple Blocks selection mode. Figure 18. Multiple blocks selection mode Select the additional 1 or 2 block(s) to run the same protocol, if the quantity of your samples is large.
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Page 26: Monitoring The Protocol Running Status
TCTT-4810 Operation Manual 6.2 Monitoring the Protocol Running Status During the running of the protocol, the running status screen will be displayed as shown in Figure 19 below. The running Step is highlighted in red (heating) or blue (cooling). Tap on different blocks to view each status. -
Page 27: Tools
Calculate the volume of the reagents needed to make the mastermix. Copy Number Convertor Convert the DNA concentration into copy number. Incubator Allows you to use TurboCycler 3 as an incubator. Help Display the quick operation guide for TurboCycler 3. -
Page 28: System Setup
Main Screen to enter the System menu as shown in Figure 21 below. Figure 21. System overview Service Mode Here you can adjust several parameters for the TurboCycler 3. Brightness 8.1.1 You can change the brightness of the display panel according to your environment‘s lighting condition. -
Page 29: Maintenance
Air vents should be vacuumed to remove dust. 9.2 Cleaning the Heated Lid Make sure the TurboCycler 3 is turned off, unplugged, and cooled down. Use mild detergents to clean material from the lid. A Kimwipe™ dipped in 70% ethanol will help remove residue from the sealing tape. -
Page 30: Troubleshooting
TCTT-4810 Operation Manual Troubleshooting Problem Cause Action Operating temperature Operate the unit in a temperature During the protoco, environment may be unsuitable. environment between 15 to 30 °C. the running status screen show warning icon Power is not reaching the Check power source voltage. -
Page 31: Error Messages
10. Troubleshooting 10.1 Error Messages Message Cause Action Error 1: Heater overheat! Lid temperature exceeds 145 Reboot the unit. °C. Error 2: Heater Lid heater problem. Reboot the unit. performance failure! Error 3: Heater Lid heater problem. Reboot the unit. temperature inaccuracy! Error 4: Block sensor Block temperature sensor... -
Page 32: Appendix A: Technical Specifications
TCTT-4810 Operation Manual Appendix A: Technical Specifications Sample Block Compatible with regular profile or low profile 0.1 ml PCR 3 X 48 well, Gradient Block tube, strip, non-skirted, and semi-skirted 48-well plate Block Temperature 4.0 – 100 °C Block Temperature Range Maximum Heating/Cooling Rate 5.0/3.0 °C/sec Average Heating/Cooling Rate... -
Page 33: Appendix B: Template Protocols
Appendix B: Template Protocols Appendix B: Template Protocols 2-Step Stage 1 94 °C, 5 min Stage 2 94 °C, 30 sec 30 cycles 67 °C, 30 sec Stage 3 72 °C, 7 min Stage 4 4 °C, Hold 3-Step Stage 1 94 °C, 5 min 94 °C, 30 sec Stage 2... - Page 34 TCTT-4810 Operation Manual Touch down Stage 1 94 °C, 5 min 94 °C, 30 sec Stage 2 60 °C (-0.5°C/Cycle), 30 sec 30 cycles 72 °C, 30 sec 94 °C, 30 sec Stage 3 45 °C, 30 sec 30 cycles 72 °C, 30 sec Stage 4 72 °C, 7 min...
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Page 35: Appendix C: Ce Declaration
5F., No.2, Aly.2, Siwei Ln., Zhongzheng Rd., Xindian Dist., New Taipei City 23148, Taiwan (R.O.C.) Declaration of Conformity Product Name: TurboCycler 3 Model Names: TCTT-4810 All models comply with the following European standards: EMC: EN 61326-1 Safety: EN 61010-1 and EN 61010-2-010 To the best of my knowledge and belief, these units conform to these standards. -
Page 36: Appendix D: Order Information
TCTT-4810 Operation Manual Appendix D: Order Information Description Cat. No. TCTT-4810 Gradient Thermal Cycler with Three Independent Blocks TCTT-a001 Tube Frame TCTT-a002 5 mm Compression mat x 3... - Page 37 Blue-Ray Biotech Corp. 5F., No.2, Aly.2, Siwei Ln., Zhongzheng Rd., Xindian Dist., New Taipei City 23148, Taiwan (R.O.C.) www.blue-raybio.com P/N: 401-T3BR00-10...
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