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Sony SH800 User Manual

Cell sorter ssff.
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ACCOUNTS MUST BE ADDED on BEFORE THE CHIP IS SCANNED
Notes:
Make sure to draw all your gates and name everything before you record your data. Once you have recorded data
you cannot draw new gates. You may copy and paste regions to new tubes.
For sort targeting with real cells
o Set the number of events to sort higher than needed
o Test placement of sorted cell by initiating SORT
o Pause and look at deposition
o It will only do one sort per tube, so if you need to do multiple tests you do not want to reach desired # of events or
it will insist on a new tube being created
1.
Start up
NOTE: The upper lid on the cytometer has pneumatic lifters, so attempting to open this with the air line
vented will create an error. The air to the cytometer should be restored before attempting to load a chip, or to
look at filters. The air line
Check the LCD display for pressure reading before opening the chip loader flap door.
1. Decontaminate and clean all surfaces of the collection chamber. And clean sort collection device if desired.
2.
No Cavicide should be used on the plates. A 70% EtOH wipe daily should be sufficient. The lower
portion of the sort chamber can be wiped with a small amount of Cavicide.
µ
i. For the 70
m chip
Microbubbles are always a greater problem for the 70um than other nozzles and may be contribute
o
to the instability. In the AM, the air line vent should be closed and the vent valve on the tank turned to
reseat the seal.
o This nozzle is VERY sensitive and side streams will not form if the plates are not clean and dry
o IF sheath pressure issue occurs, check the lid of the sheath tank.
January 11,2018
Sony SH800 Cell Sorter
Currently the 70, 100 and 130 µm chips are in use
SSFF
1

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Table of Contents

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   Summary of Contents for Sony SH800

  • Page 1 Sony SH800 Cell Sorter SSFF ACCOUNTS MUST BE ADDED on BEFORE THE CHIP IS SCANNED Currently the 70, 100 and 130 µm chips are in use Notes: Make sure to draw all your gates and name everything before you record your data. Once you have recorded data •...
  • Page 2 4. Turn pressure on before continuing on to any other steps. 5. Power up the SH800. The display screen will show the active levels of fluids. 6. Disconnect the airline in from the sheath tank. Release pressure using pressure relief ring. Fill sheath tank to below the upper weld line.
  • Page 3 iv. If this does not resolve issue, check the optical filters, sheath tank seal, check front cytometer pane for liquid levels. 3. If you fill the di water bottle you will need to de-bubble the di water filter using the option in the software. 4.
  • Page 4 5. Sorting into Tubes 1. The Sony pre-calculates the sort stream voltage and angle automatically. If you have large cells, or want to test the sort stream location before sorting: i. Open “settings” option in the cytometer ribbon ii.
  • Page 5 6. Select the Plate Adjustment tab. 7. Select ‘4 corners and center’ 8. Click ‘Start’ and the SH800 will sort 50 drops into the corner wells and a center well. 9. When it finishes, the plate will unload and you can remove it to check the drop positions over the wells.
  • Page 6 To put the machine in standby mode: 1. From the cytometer ribbon, select “settings” (a) 2. On the settings panel, select “advanced settings” 3. On the “pressure options” tab, select “standby” (b) 4. Once you have replaced the tank after emptying and adding bleach, or filled the sheath tank select “ready” (c) 7.
  • Page 7 4. Spray them with cavicide, then rinse them with hot water and then spray them with alcohol. Leave them on clean diaper to dry 5. While cleaning, spray the counters, mouse and keyboard with cavicide 6. After the bleach has finished running there is a 2 minute probe wash 7.
  • Page 8 " V450 " Live/Dead Violet This SH800 Sony Sorter has NO Laser. These " Zombie Violet colors will get sub-optimal excitation with the 561 " eGFP FITC " Yellow Green Laser. It is recommended that you use " Alexa Fluor 488 one of the dyes when given the opportunity.

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