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Summary of Contents for Thermo Electron BBD 6220
- Page 1 Operating instructions Hot-Air Disinfectable Gassed Incubator BBD 6220 Read this operating instructions and keep it near the equipment for reference purposes ! Valid: 03.2006 / 50 079 041 A...
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Page 2: D - 63505 Langenselbold
As regards foreign-language translations, the German version of this instruction manual is binding. This edition of the instruction manual applies to the BBD 6220 unit specified on the front page. Date of publication: 03.2006 Nominal charge Safety with regard to the protection of persons, the environment and the material under process largely depends on the conduct of the persons using the equipment. -
Page 3: Table Of Contents
LIST OF CONTENTS PAGE 1. GENERAL ................5/7 Explanation of icons in the instructions manual . - Page 4 LIST OF CONTENTS PAGE 7. START-UP ..................32 8.
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Page 5: General
1. GENERAL EXPLANATION OF ICONS IN THE INSTRUCTIONS MANUAL In chapters of the instruction manual which deal with safety, this icon appears under the title of the chapter. Displayed on the equipment, this icon denotes that special at- tention must be paid to the information given in the instruction manual or accompany- ing documents. -
Page 6: Explanation Of Icons In The Short Instructions
1. GENERAL EXPLANATION OF ICONS TO THE SHORT INSTRUCTIONS Switch on unit (operate the main switch) max. Check the water level aqua dest. min. > + 2 °C Set the temperature limit controller °C Close unit doors & Read the operating instructions start/ Start "disinfection routine"... -
Page 7: General Instructions
1. GENERAL General instructions To avoid errors and causing damage, especially personal injuries, be sure to read this manual carefully before using the equipment, and follow all instructions. When setting up and operating the unit, be sure to comply with the instructions contained in this manual as well as all applicable laws, regulations and directives in your country (FRG: ZH 1/119, ZH 1/342, ZH 1/343, ZH 1/598). -
Page 8: Areas Of Application
2. AREAS OF APPLICATION The BBD 6220 gassed incubator is an item of laboratory equipment for cell and tissue cultures and can be used to simulate the physiological environmental conditions of cells. The unit is generally suitable for setup and operation in the following areas: In laboratories for microbiological and biotechnological work. -
Page 9: Safety Instructions
3. SAFETY INSTRUCTIONS In the case of biological incubators, biological safety with regard to the protection of persons, its surroundings and the load is heavily dependent on the observance of the applicable regulations by the persons using the equipment. Even then, however, the possibility of hazards, especially health hazards, arising cannot be ruled out. -
Page 10: Setup And Installation
4. SETUP AND INSTALLATION When setting up, installing and operating the unit, be sure to comply with all applicable regulations in your country. (FRG: ZH 1/119, ZH 1/342, ZH 1/343, ZH 1/598) Transport Handle the unit with care during transport. Do not lift up the unit by its door. Refer to TECHNICAL SPECIFICATIONS for weights and dimensions. -
Page 11: Clearances
4. SETUP AND INSTALLATION Clearances When setting up the unit, the minimum clearances between the unit and adjacent surfaces and other units must be maintained. Larger clearances are recommended to facilitate accessibility for installation and the supply con- nections. Fig. 1: Minimum clearances in mm Supply connections Mains socket C0 gas connection... -
Page 12: Gas Supply Connection
4. SETUP AND INSTALLATION Gas supply connection The gas connection are located on the back of the unit. The gas must be supplied to each unit via a pressure reducer with the inlet pressure set to between 0.8 and max. 1 bar. This setting must not be changed for safety reasons (FRG: ZH 1/119). -
Page 13: Description Of The Unit
5. DESCRIPTION OF THE UNIT Levelling the shelves The shelves can be aligned with the aid of a spirit level with height-adjustable feet. Functional principle The atmosphere in the chamber can be regulated in relation to its temperature, CO concentra- tion, O concentration (optional) and relative humidity. -
Page 14: Humidification
5. DESCRIPTION OF THE UNIT Humidification The atmosphere in the chamber is humidified through the admission of steam generated in a aktiv humifier. Relative humidity (rH) can be regulated between 60% and 95%. To ensure reliable operation of the steam generator, only fill the reservoir with fully desali- nated or distilled water! The capacity of the reservoir is approx. -
Page 15: Operate
6. OPERATE Switch panel ( 5 ) (A) "Control and display panel" (refer to Fig. 7) (1) Indicator lamp "Fault, overtemp. protection" (2) Indicator lamp "Disinfection mode" Heraeus (3) Indicator lamp "Master Switch" (4) Vent/overflow "Water supply" & autozero (5) Level gauge "Water supply"... -
Page 16: Water Supply
6. OPERATE Water supply Level gauge (5) indicates the water level. The water level should be in the range between "min and max". This range is equivalent to a volume of 2.2 ltr. If the water supply falls below the "min." level, a reserve of 0.5 ltr. is still available. -
Page 17: Mains Switch
6. OPERATE As the outlet not only functions as an overflow but also as a vent, it must be unsealed while the unit is in operation. To drain the empty filling canister, place it on the floor and connect it using the quick- release coupling (9). -
Page 18: Temperature Protection Device (Temperature Limit Controller)
6. OPERATE Temperature protection device (temperature limit controller) A temperature protection device is built into the unit and acts as a temperature limit controller. It conforms to Thermal Safety Class 3.1 to DIN 12880 Part 1/11.78. In a functional state, the temperature limit controller assumes the control function if the set temperature is exceeded. - Page 19 6. OPERATE (11) ƒ& (12) autozero (13) &2 (14) (15) (16) (17) auto-start (18) (19) (10) control (20) Fig. 7: Control and display panel (A) (1) Indicator lamp "HEATING" (11) Indicator lamp "Disinfection mode" (2) Display "Temperature" (12) Key "Temperature setpoint"...
- Page 20 6. OPERATE In its as-delivered condition, the unit is preset to the following values: Temperature: 37.0°C concentration: 0.0% concentration: 21.0% (optional) Relative humidity: 60.0%, humidity control activated via function level 2 Setting the temperature setpoint: Instruction Entry / Keys / Remarks Display / Remarks / Status Example: Switch on unit...
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Page 21: Disinfection Routine
6. OPERATE Setting the relative humidity setpoint: Instruction Entry / Keys / Remarks Display / Remarks / Status Display the relative humidity Actual setpoint is displayed and press setpoint decimal point flashes Set the relative humidity set- Setpoint increases press &... - Page 22 6. OPERATE Disinfection Temp. Heating phase Cooling phase phase 180°C 37°C Time Start Flow chart of disinfection routine: Status display on CO display: Heat: The chamber is heated to 180°C; the disinfection temperature can be read off the temperature display. Disinfect: When the disinfection temperature of 180°C is reached, a disinfection phase with a duration of approx.
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Page 23: Auto-Start Routine / Auto-Zero Routine
6. OPERATE auto-start routine / auto-zero routine An auto-start routine / auto-zero routine will have to be run when putting the unit into operation or if the temperature setpoint is altered by more than 1°C. To ensure that there is only air, i.e. no CO in the chamber before the auto-start routine is acti- vated, ventilate the chamber by opening all doors. - Page 24 6. OPERATE Instruction Entry / Keys / Remarks Display / Remarks / Status Cancel auto-start routine Indicator lamp (page 19 (17)) "auto- & start" goes out and auto-zero cali- bration routine starts Press for approx. 5 seconds Indicator lamp (page 19 (13)) "auto- zero"...
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Page 25: Error Codes
Contact service Relative humidity be- Actual value < setpoint -5% Check the water reservoir low setpoint Contact the service department of Thermo Electron Gmbh if necessary Relative humidity sen- Sensor faulty/short- Contact the service department of sor faulty circuited... - Page 26 Electron Gmbh if necessary Actual value > 180°C Contact the service department of "des" temperature +10°C Thermo Electron Gmbh above setpoint Error during disinfec- Power failure > 1 sec Press the des buton (Fig.4, (7)) to re- tion procedure set the error and repeat the disinfec- tion procedure;...
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Page 27: Switching Functions
6. OPERATE Switching functions Press - Key (page 19 (17)) to select the individual switching functions as required. The indicator lamp "control" (page 19 (16)) indicates when one of the switching functions specified below is selected: Acoustic alarm ON/OFF Humidification ON/OFF Manual zero calibration of CO measuring system Setpoint lock-in ON/OFF... -
Page 28: Switching Function: Humidification On/Off
6. OPERATE Switching function: Humidification ON/OFF Factory setting: Humidification switched on (set to 60 % rel. humidity). Instruction Entry / Keys / Remarks Display / Remarks / Status Select function level 2 Indicator lamp "control" flashes Hold down and current function level is indi- cated by the flashing display °C Press... -
Page 29: Switching Function: Manual Zero Calibration Of Co 2 Measuring System
6. OPERATE Switching function: Manual zero calibration of CO measuring system Recommendation: Check the CO concentration, especially during long-term tests. If there is a large deviation (± 0.5 %) between the displayed actual value for CO concentration and the nominal value, the measuring system can be recalibrated during operation. Example: Display: 5.0 % CO... -
Page 30: Switching Function: Setpoint Lock-In On/Off
6. OPERATE Switching function: Setpoint lock-in ON/OFF To prevent unintentional or unauthorized modification of the setpoints for temperature, CO and rel. humidity, the setpoints can be "locked in". The setpoints cannot be changed until the interlock has be cancelled again. Factory setting: setpoints unlocked. -
Page 31: Switching Function: Door Heater On/Off
6. OPERATE Switching function: Door heater ON/OFF To adapt the unit to the ambient temperature in the place of installation, the door heater can be switched on or off (see page 13 for example). Condensation can occur inside the unit, especially on the glass door or glass screen, when the door heater is switched off. -
Page 32: Start-Up
7. START-UP The items specified in brackets refer to Fig. 4 on page 15. Instruction Entry / Keys / Remarks Display / Remarks / Status Open unit doors Remove transit restraints and accessories from inside the unit Carry out basic cleaning Water reservoir Fill unit with water -- observe max. -
Page 33: Operation
8. OPERATION Operating control The microbiological safety of the unit is largely dependent on the proper conduct of the persons using the unit. Refer to the Appendix "Basic rules of sound microbiological engineering practice". Be sure to comply with the instructions contained in this manual as well as all applicable regula- tions and directives in your country (FRG: ZH 1/119, ZH 1/342, ZH 1/343, ZH 1/598). -
Page 34: Shutdown
9. SHUTDOWN The unit may only be shut down by qualified staff trained to operate this equipment. The unit must not present any risk of contamination after shutdown. Shut off the gas supply. Remove, disinfect and clean any objects or auxiliaries put into the unit. Disinfect and clean the chamber. -
Page 35: Maintenance
Do not spray the O / rel. humidity sensors (porous sintered material) with disinfectant. Recommended disinfectant: A surface disinfectant recommended by Thermo Electron Corporation can be ordered under following part numbers: Spray bottle, 250 ml Part No.: 50052425 Refill bottle, 500 ml Part No.: 50051939 NOTE - Decription disingectant: Details for efficiency and approvals are available on request. -
Page 36: Testing
Testing The serviceability and safety of the unit are only guaranteed if the necessary testing, maintenance and repair work is carried out by the Thermo Electron Gmbh department or by staff authorized by us. The unit should be checked for safety, leakproofing and serviceability at least once a year. -
Page 37: Authorized Equipment Parts And Accessories
11. AUTHORIZED REPLACEMENT PARTS AND ACCESSORIES The safety and serviceability of the equipment are only guaranteed if the approved original replacement parts specified below are used. Use of other parts presents unknown risks and is not approved under any circumstances. Replacement part Type Order No. -
Page 38: Technical Specifications
12. TECHNICAL SPECIFICATIONS TECHNICAL SPECIFICATIONS UNITS: VALUE: MECHANICAL: Outer dimensions (W x H x D) 920 x 855 x 775 Inner dimensions (W x H x D) 607 x 669 x 585 Chamber volume approx. 220 Divided Shelves (W x D) 260 x 500 Quantity included in scope of supply Max. - Page 39 12. TECHNICAL SPECIFICATIONS TECHNICAL SPECIFICATIONS UNITS: VALUE: Humidification Water quality Distilled or fully desalinated min. - max. level Water demand to humidify: from 40 % rel. humidity to 95 % rel. humidity Water consumption ml/h Total capacity Measuring range and Setting range* % r.H.
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Page 40: Materials Used / Disposal
Member State, and this product should be disposed of or recycled through them. Further information on Thermo Electron`s compliance with these Directives, the recyclers in your country, and information on Thermo Electron products wich may assist the detection of substances subject to the RoHS Directive are available at www.thermo.com/WEEERoHS. -
Page 41: Gas Consumption
14. GAS CONSUMPTION Gas consumption during operation Gas consumption corresponds to the gas quantity re- of "BBD 6220 gassed incubator" leased at the back of the unit which has to be discharged safely into the outside air by ventilating the placement area. -
Page 42: Ph-Value Of Culture Media
PH-VALUE OF CULTURE MEDIA pH-value of culture media Dependency on the CO2-concentration Media Na HCO DMEM 3,70 2,20 2,20 Medium 199 2,20 Mc Coy 2,20 F 10 1,20 3,70 F 12 1,20 0,85 Media with Hanks Salts 0,35 2,20 1,20 0,85 0,35 pH-value... -
Page 43: Basic Rules Of Sound Microbiological Engineering Practice
BASIC RULES OF SOUND MICROBIOLOGICAL ENGINEERING PRACTICE * General rules Keep windows and doors in the working areas closed while work is in progress. Do not consume drinks, food or smoke in the working areas. Do not keep foodstuffs in the working areas. Lab coats or other protective garments must be worn in the working area. -
Page 44: Handling Of Human- And Animal-Pathogenic Biological Agents
BASIC RULES OF SOUND MICROBIOLOGICAL ENGINEERING PRACTICE * Potentially hazardous, genetically engineered organisms, viruses and subviral agents must be handled in accordance with employers´ liability insurance association guideline G 43 entitled "Biotechnology". Instructions for administering first aid in the event of accidents involving pathogenic microorganisms and viruses must be immediately to hand in the working area. - Page 45 Internet: www.thermo.com 50 079 041 45/45...
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