Self-Calibration Of Optical Circuits - Chrono-Log 490 4+4 Instruction Manual

A Soft, Lint-Free Cleaning Cloth -- Moisten the cleaning cloth with
the dishwashing detergent solution to clean the exterior of the
Aggregometer.
A Small Vacuum Cleaner with a Brush attachment -- Use the
vacuum cleaner to remove dust and dirt from the exterior of the
Aggregometer.
Cleaning the Aggregometer
1. Turn off the Aggregometer and any attached peripherals.
Disconnect them from the Aggregometer. Unplug the
instrument before cleaning to prevent a shock hazard should
any fluid enter the power switch.
2. Use a vacuum cleaner to remove any dust from the slots and
holes in the Aggregometer.
3. Moisten a soft cleaning cloth with a solution of three parts
water and one part liquid detergent.
NOTE -- DO NOT SOAK THE CLOTH IN THE SOLUTION; DO NOT
LET SOLUTION DRIP INSIDE THE AGGREGOMETER.
4. Use the moistened cloth to wipe the Aggregometer exterior.

SELF-CALIBRATION OF OPTICAL CIRCUITS

This procedure enables the Optical wells (PRP & PPP) to be
calibrated automatically with the push of a button and verifies
that the instrument's optical circuits are operating properly.
During the performance of this procedure, the light intensity of
the LEDs are adjusted and set to the proper calibrated voltage.
Use a stop watch or timer since some of the steps must be timed.
The calibration switch located on the front of the instrument is
key-activated. This is to prevent unauthorized use or accidental
initialization of the procedure. DO NOT LEAVE THE KEY IN THE
SWITCH WHEN NOT PERFORMING THE CALIBRATION
PROCEDURE. The key should be stored in a safe location
accessible only to personnel authorized by laboratory
management. If the key is lost, a replacement key may be
purchased from the factory. Please note your Key # here
____________________.
Failure to follow this procedure exactly may result in an
inaccurate calibration. The Post Calibration Test checks the
accuracy of the calibration and enables the operator to have a
record of the results. This calibration procedure is not a
substitute for the recommended yearly instrument calibration
and PM.
Preparation:
1. Materials Required
P/N 322. Calibration Kit with (1) black cuvette and (2)
a.
sealed water cuvettes.
Lint-Free Wipe
b.
Calibration Key
c.
2. Turn on the unit and let it heat up until the heater blocks
stabilize at 37°C.
3. Since each Channel is calibrated independently, confirm
that that PPP/Reference Select Switches are set to
reference each Channel's PPP sample, and are not
referencing Channel #1 PPP.
Document # 49044IM1
Revision 7.5
Dated February 16, 2017
NOTE: Additional steps required for CHRONO-LOG® instruments
that have a PPP Reference switch allowing multiple channels to
reference the PPP in Channel. [See "Additional Post Calibration
test for Instruments with PPP Select Switches" on Page 19 ]
4. Place the (2) water cuvettes from P/N 322 in the incubation
wells and pre-warm for a minimum of five (5) minutes. Be
sure to always wipe cuvettes with Kim Wipe® before placing
in test or reference wells.
5. Turn on computer and Start AGGRO/LINK® Opti8
Windows ® program.
6. Under the AGGREGOMETER window, select or set-up a Test
Procedure page for Optical mode. Click on OK.
7. Select RUN NEW PATIENT under AGGREGOMETER and Click
on OK.
8. Type, "Optical Calibration Pre", in Test Identification box at
top of screen. Click on OK and test will begin running.
9. After the five (5) minute incubation, check cuvettes for air
bubbles. Eliminate bubbles by tapping the cuvette.
Pre-Calibration Test
NOTE: Throughout the entire procedure, the Water Cuvette used
in the PRP well should only be used in PRP test wells and the PPP
Water Cuvette should only be used in PPP reference wells. Be
sure to use the Alignment Mark on side of each cuvette to ensure
consistent placement of cuvettes into test and reference wells.
1. Click on Aggregometer, select Reset Channel and select ALL
Channels. Click on OK.
2. After wiping the outside with a Kim Wipe®, place BLACK
cuvette in Channel 1 PRP well and a water cuvette in the PPP
well of Channel 1, with the alignment mark placed left
center.
3. Click on the Channel 1 "Activate" bar. [NOTE: If this pre-test
is to be SAVED, Click on the "Start" Bar for Channel 1. If not
saving, the following steps can be performed after clicking
on the "Activated" button. ]
4. Push and Hold the Set Baseline button until the tracing
reaches a full 100% of graph before releasing. Tracing will
then return to 0% of graph.
5. Replace the BLACK cuvette in the PRP well with the other
water cuvette, after wiping the outside with a Kim Wipe®.
Be sure alignment mark is placed left center. The tracing
should move near 100% on the graph. Allow to run for a
few seconds and note the position where the tracing
stabilizes. (Ex: 97%)
6. Repeat Steps 2 through 5 above for Channel 2, and then
continue to repeat for Channels 3, 4, 5, 6, 7 and 8. Be sure
to use the water cuvette in the PPP well in all PPP wells and
the water cuvette in the PRP well in all PRP wells. Also be
sure to wipe the outside with a Kim Wipe® and to place the
Alignment Mark to the left center of the test well.
7. If saving the test ... Once all channels are completed, Click on
the STOP icon followed by the SAVE icon and the PRINT icon
to save and print the test.
8. If any tracing is > 4% from 0% or 100% on the graph,
proceed with calibration steps below, calibrating all eight (8)
channels.
17
TM for