Chrono-Log 490 4+4 Instruction Manual page 11

be further diluted in isotonic glucose pH 2.7. Do not
dilute entire bottle, only enough for a day's testing.
Add 1 µL of reagent to 500 µL sample or 0.5 µL of
reagent to 250 µL sample for a final concentration of
2 µg/mL. Normal aggregation is seen with final
concentrations of 1-5 µg/mL.
Stability: Collagen does not contain any preservative,
but because of its very low pH, organisms do not grow
as readily. If asceptic techniques are used (sterile
syringe and needle to remove one day's use),
remaining reagent, if stored at 2 - 8°C, is stable until
expiration date. Reagent removed from the vial is
stable for one week at 2-8°C.
f. Epinephrine
Catalog No.: 393
Supplied As: Lyophilized preparation of l-Epinephrine
bitartarate with stabilizers
Stock Conc.: 10 mM for Whole Blood Testing; 1mM for
PRP testing
Stock Storage: Refrigerate at 2-8°C
Stock Shelf life: Until expiration date
Working Conc.: 1 mM for PRP testing
Working Storage: 2-8°C in dark container
Working Shelf life: 8 hours (in the dark)
Reconstitute with 5.0 mL sterile distilled water (dilute
1:10 with physiological saline for PRP testing).
Preparation: Tap vial gently to get contents to the
bottom. Remove stopper and reconstitute with 5.0 mL
sterile, distilled water. Dilute the stock 1:10 with
physiological saline for PRP testing. Allow to sit for ten
minutes with occasional inversion. Adding 5µL of 1:10
Diluted Solution to 500 µL sample of platelet rich
plasma or 2.5 µL of reagent to 250 µL sample gives a
final concentration of 10 µM. Normal aggregation is
seen with final concentrations of 5-10 µM in platelet
rich plasma.
Stability: Epinephrine is a comparatively unstable
reagent. The unused reconstituted Epinephrine can be
stored frozen at -70°C in the dark and in 100 µL
aliquots for 3 months or until the expiration date,
whichever comes first.
NOTE: Normal subjects exhibit considerable variability that is
not correlated with age, sex, stress, diet, platelet count or
hematocrit.
g. Ristocetin
Catalog No.: 396
Supplied As: 62.5 mg of stabilized freeze dried
Ristocetin.
Stock Conc.: 125 mg/mL
Stock Storage: Refrigerate at 2-8°C (in the dark)
Stock Shelf Life: Until expiration date
Working Conc.: 125 mg/mL
Working Storage: 2-8°C
Document # 49044IM1
Revision 7.5
Dated February 16, 2017
Working Shelf Life: 8 hours (in the dark)
Reconstitute with 0.5 mL of sterile distilled water.
Preparation: Tap vial gently to get contents to the
bottom. Remove stopper and reconstitute with 0.5 mL
of sterile distilled water. Do not shake or invert vial.
Re-stopper and allow to sit for 10-15 minutes. Visually
inspect bottom of vial to confirm reagent is fully in
suspension. Do not shake the reagent, invert gently, to
take up any reagent remaining in stopper and allow
vial to sit for another 10-15 minutes until all particulate
matter is well dissolved. Reagent may have a clear-to-
brownish color suspension after reconstitution. Never
shake reagent. Swirl gently just before use.
Add 5 µL of reagent to 500 µL PRP sample or 2.5 µL of
reagent to 250 µL sample for a concentration of 1.25
mg/mL. Normal aggregation is seen with final
concentration of 0.63 - 1.5 mg/mL.
Stability: The unused reconstituted Ristocetin reagent
can be stored frozen at -20°C in volumes suitable for a
days testing for 3 months or until the expiration date,
whichever comes first. DO NOT STORE AT -70
4. AGGRO/LINK® Opti8
a. Turn on Computer and Start AGGRO/LINK® Opti8
®
Windows program. Be sure "Aggregometer Ready"
appears in the bottom right-hand corner of the screen.
If not, check USB Port, cables and connectors. If
USB/Com Port is changed, go to AGGREGOMETER then
CONNECT.
b. Click on EDIT and CONFIGURATION. Type in your
institutions information under Report Header. This will
be printed out on top of Report Format when Report
Batch Print is selected under File. Click on OK when
completed.
c. To have Area Under Curve and Lag Time Calculated
and printed on test data, click on the box to select. To
disable, leave this box unchecked.
d. To display and print Start Indicators, click on the box to
select. To disable, leave this box unchecked.
e. Under the AGGREGOMETER window, select or set-up a
test Procedure page for Optical mode. To save the
procedure for future use, change the name in the
Procedure Name field. Click on OK and select Run New
Patient under AGGREGOMETER.
NOTE – AGGRO/LINK® Opti8
• CREATE MERGE – Creates a blank document for pasting
curves
• EXPORT - Export test data for use with another program
• ADJUST SLOPE LINE – Change slope calculation time
OFFSET CURVES – Change the physical start time of a test
•
Channel
• COPY SCREEN – Copies the test grid and curves to the
system Clipboard
• MERGE COPY ALL or SELECTED - To copy all the curves from
a file or to copy only selected curves from a file to a merge
document.
START & STOP TIMES – Automatic marking at addition of
•
reagent.
• CUSTOM COLOR SETUP – Use Default tracing colors or
customize
10
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