Table of Contents
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INSTRUCTION MANUAL
FOR THE
®
CHRONO-LOG
PLATELET AGGREGOMETER
MODEL 490 4+4
490 4+ Four Channel Optical Aggregometer
490 4+4 Eight Channel Optical Aggregometer
FOR IN-VITRO DIAGNOSTIC USE
For Measuring platelet aggregation in platelet rich plasma
● 2 West Park Road ● Havertown PA 19083
● TEL 800-247-6665 ● 610-853-1130
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Table of Contents
Summary of Contents for Chrono-Log 490 4+4
- Page 1 CHRONO-LOG PLATELET AGGREGOMETER MODEL 490 4+4 490 4+ Four Channel Optical Aggregometer 490 4+4 Eight Channel Optical Aggregometer FOR IN-VITRO DIAGNOSTIC USE For Measuring platelet aggregation in platelet rich plasma ● 2 West Park Road ● Havertown PA 19083 ● TEL 800-247-6665 ● 610-853-1130...
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Page 2: Table Of Contents
TABLE OF CONTENTS Manufacturer/EC Rep Details Instrument Function Verification Power Requirements / Fuses Operating Instructions Warnings Specimen Collection Ventilation Equipment & Materials Hazards Preparation Misuse Calibration Summary and Explanation Quality Control Overview of Hemostasis Procedure Stepwise Abnormalities of the hemostatic Calculations mechanism Platelet function... -
Page 3: Manufacturer/Ec Rep Details
Manufacturer: Chrono-log Corp. Connect the equipment into an outlet on a circuit different • 2 West Park Road from that to which the receiver is connected. Havertown, PA 19083 • Consult the dealer or an experienced radio/TV technician for help. -
Page 4: Overview Of Hemostasis
patients are not routinely screened for platelet defects despite hemostatic balance. These defects can be hereditary or they can the fact that many cases of platelet dysfunction are discovered be acquired, through a pathological process, for example. Platelets - disorders of the platelet component of hemostasis can only after excessive or recurrent post-surgical bleeding. -
Page 5: Principles Of Platelet Aggregation
The selection of these reagents has some basis in theory. Both The CHRONO-LOG® sample chambers are designed so that a ADP and epinephrine (adrenaline) are contained within the beam of infra-red light shines through two cuvettes, one... -
Page 6: Instrument Specifications
Intended Use • Stir Bars - reusable teflon coated P/N 313, disposable P/N The CHRONO-LOG® Model 490 4+4 Aggregometer is intended for • Incubation Wells – two (2) wells for each channel for P/N use for in-vitro diagnostic use for measuring Platelet Aggregation 312 cuvettes at 36.5°... -
Page 7: Instrument Controls
Computer Connections: (See Connection Charts in Appendix • Windows-based PC with a Pentium/compatible processor B ) If the computer was purchased through Chrono-log the running at 133 MHz or higher. software was pre-installed, proceed to step 9. •... -
Page 8: Instrument Function Verification
Heparin, Warfarin, Direct Thrombin Inhibitors (lepirudin, OPERATING INSTRUCTIONS argatroban, bivalirudin) • Cardiovascular Agents For Model 490 4+/490 4+4, install the AGGRO/LINK® Opti8 β-adrenergic blockers (propranolol), Vasodilators Software as described previously under INSTALLATION section of (nitroprusside, nitroclygerin), Diuretics (furosemide), this manual. -
Page 9: Equipment & Materials
Put cuvettes containing stir bar in the incubation wells during sample preparation. to warm up. When the Model 490 4+4 test channels are set to Handling Conditions: Reference Channel 1, a single sample of PPP can be Testing can start 30 minutes after venipuncture and continue for used as the reference sample for all tests run with the about 2.5 hours after. - Page 10 Stock Storage: Frozen below -20°C for Arachidonic Acid, Water Refrigerate at 2-8°C for albumin. Catalog No.: 398 Stock Shelf Life: Until expiration date. Supplied As: Purified Water 15mL Working Conc.: 50 mM Sterile distilled, bottled water suitable for CHRONO- Working Storage: 2-8°C [in the dark] PAR®...
- Page 11 be further diluted in isotonic glucose pH 2.7. Do not Working Shelf Life: 8 hours (in the dark) dilute entire bottle, only enough for a day’s testing. Reconstitute with 0.5 mL of sterile distilled water. Add 1 µL of reagent to 500 µL sample or 0.5 µL of Preparation: Tap vial gently to get contents to the reagent to 250 µL sample for a final concentration of bottom.
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Page 12: Calibration
ACTIVATE Bar – provides the ability to control each test Remove the tip slowly from the liquid. For large • individually or in sets of 2 or 4 channels, eliminating the volumes, wait approximately 3 seconds before risk of some samples sitting in test well for extended removing the tip from the liquid. - Page 13 5 minutes. Slope length can be set from 16 to 14. Repeat steps 12 and 13 for each test channel. 99 seconds. Chrono-log standard setting is 16 seconds. Click 15. Allow all tests to run for a minimum of five (5) minutes.
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Page 14: Calculations
To change the length of a slope line, click on EDIT, then ADJUST SLOPE LINE. Click on Trace number and use arrows to adjust slope length up to 99 seconds. Chrono-log standard setting is 16 seconds (32 points). REPORTING RESULTS: Report Hematocrit. - Page 15 AGGREGATION RESPONSE WITH SELECTED ABNORMALITIES Final Von Willebrand & Storage Pool/ Reagent Aspirin Effect Glanzmann's Thrombasthenia Concentration Bernard Soulier Secretion Defect N, R * N, R * 5 – 10 µ µ µ µ M Arachidonic Acid 0.5 mM 1 or 2 5 µ...
- Page 16 Reporting Format: PLATELET AGGREGATION STUDIES: DATE __________________________________ PHYSICIAN _________________________________ TIME BLOOD DRAWN: ___________ INSTITUTION: ______________________________ DEPARTMENT: ____________________ PATIENT ___________________ I.D. _______________ DATE OF BIRTH: ______________ AGE: ________ SEX: (M) (F) HCT ___________ PLT CT _________ BT _________ CLINICAL HISTORY: ______________________________________________________________________________________ ________________________________________________________________________________________________________ ________________________________________________________________________________________________________ OPTICAL AGGREGATION TEST RESULTS...
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Page 17: Interpretation
Protective Earth Ground: The instrument is connected to ground at the instrument chassis. This sole purpose connection point is With the Model 490 4+4, spacers are not required when made between the power entry module and the chassis with testing micro-volume samples (250 µL of PRP) and all green/yellow wire and is marked with the IEC 60417-5019 CHRONO-PAR®... -
Page 18: Self-Calibration Of Optical Circuits
A Soft, Lint-Free Cleaning Cloth -- Moisten the cleaning cloth with NOTE: Additional steps required for CHRONO-LOG® instruments the dishwashing detergent solution to clean the exterior of the that have a PPP Reference switch allowing multiple channels to Aggregometer. reference the PPP in Channel. [See “Additional Post Calibration test for Instruments with PPP Select Switches”... - Page 19 If, after the second attempt, the results are move the samples. Moving the samples may cause > 4%, the system may need Service. Contact Chrono-log Service inaccuracies in the calibration. Wait for the one minute [or Department at 1-800-247-6665 for further assistance.
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Page 20: Troubleshooting Guide
If, after the second attempt, the results are Assistance and Distributor Information still > 4%, the system may need service. Contact Chrono-log Service Department at 1-800-247-6665 for further assistance. For Assistance on training, service, software updates and other... -
Page 21: Bibliography
BIBLIOGRAPHY Born, GVR: Quantitative investigation into the aggregation of blood platelets. J. Physiol. London 162, 67, 1962. Genton E, Gent M, Hirsh J, and Harker LA: Platelet- Inhibiting drugs in the prevention of clinical thrombotic disease. New Eng. J. Med. 293: 1174-1178, 1236-1240, 1291-1300 (4, 11 and 18 Dec.) 1975. -
Page 22: Appendix A
If the difference between the two numbers is > 4%, the two Water cuvettes are not matched and should be replaced. Contact Chrono-log to order P/N 322 Calibration Kit containing (1) BLACK Cuvette and (2) sealed Water Cuvettes. NOTE: If it is determined that Calibration cannot be delayed to wait for delivery of a new Kit, standard P/N 312 cuvettes can be substituted, as described in Step 2. -
Page 23: Appendix B
APPENDIX B Document # 49044IM1 Revision 7.5 Dated February 16, 2017...