Esco CelCulture CO2 User Manual
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Esco CelCulture CO2 User Manual

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Thank you for purchasing this Esco CO
this manual thoroughly to familiarize yourself with the many
unique features and exciting innovations we have built into
your new equipment. Esco provides many other resources at
our website, www.escoglobal.com, to complement this manual
and help you enjoy many years of productive and safe use of
your Esco products.
Esco Technologies, Inc.
2940 Turnpike Drive, Units 15-16 • Hatboro, PA 19040, USA
Toll-Free USA and Canada 888-375-ESCO
Tel 215-441-9661 • Fax 215-441-9660
us.escoglobal.com • usa@escoglobal.com
Esco Micro Pte. Ltd.
21 Changi South Street 1 • Singapore 486 777
Tel +65 6542 0833 • Fax +65 6542 6920
www.escoglobal.com • mail@escoglobal.com
Incubator. Please read
2
User
Manual
CelCulture

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Summary of Contents for Esco CelCulture CO2

  • Page 1 Esco provides many other resources at our website, www.escoglobal.com, to complement this manual and help you enjoy many years of productive and safe use of your Esco products.

  • Page 2: Copyright Information

    Esco makes no representations or warranties as to the accuracy of the information contained in this manual. In no event shall Esco be held liable for any damages, direct or consequential, arising out of or related to the use of this manual.”...

  • Page 3: Table Of Contents

    Introductory Page Table of Contents Warranty Terms and Conditions Introduction 1. Products Covered 2. Safety Warning 3. Limitation of Liability 4. European Union Directives on WEEE and RoHS Declaration of Conformity Chapter 1 – Product Information 1.1 About CelCulture CO Incubator 1.2 Labels 1.3 Quick View...
  • Page 4 Chapter 4 – General Maintenance 4.1 Scheduled Maintenance 4.1.1 Check CO Gas Tank Level 4.1.2 Check Water Level in the Humidity Pan 4.1.3 Cleaning and Decontamination 4.1.4 General Inspection 4.1.5 Calibration of Temperature, CO and Humidity 4.1.6 Change ULPA Filter 4.1.7 Change Inline Filter 4.2 Maintenance/Service Log Chapter 5 –...
  • Page 5 Esco that these parts were defective at the time of being sold, and that all defective parts shall be returned, properly identified with a Return Authorization.
  • Page 6 3 years limited. 60 months on Compressor. The warranty period starts two months from the date your equipment is shipped from Esco facility for international distributors. This allows shipping time so the warranty will go into effect at approximately the same time the equipment is delivered to the user.

  • Page 7: Products Covered

    The disposal and / or emission of substances used in connection with this equipment may be governed by various local regulations. Familiarization and compliance with any such regulations are the sole responsibility of the users. Esco’s liability is limited with respect to user compliance with such regulations. 4. European Union Directive on WEEE and RoHS The European Union has issued two directives: •...
  • Page 8 CelCulture...
  • Page 9 Low Voltage : EN 61010-1:2010 : EN 61326-1:2006 Class B More information may be obtained from Esco’s authorized distributors located within the European Union. A list of these parties and their contact information is available on request from Esco _______________________________...
  • Page 10 viii CelCulture...

  • Page 11: About Celculture Co Incubator

    1.1 About CelCulture CO Incubators A CO Incubator is a device for controlling the temperature, humidity, CO₂ level, and other conditions in which a cell culture is being grown or maintained. Incubators are essential for experimental work in cell biology, microbiology and molecular biology and are widely used in scientific research to grow and maintain cell cultures.

  • Page 12: Quick View

    1.3 Quick View 1.3.1 Front Quick View 1. Top Cover 2. Door switch 3. Circulation fan 4. ULPA filter 5. Shelves 6. Sampling port 7. Glass door gasket 8. Humidity water pan 9. Leveling feet 10. Control panel 11. Outer door 12.

  • Page 13: Back Quick View

    1.3.2 Back Quick View 1. Main switch 2. Power inlet 3. RS485 communication 4. Analog output (optional) 5. Alarm contacts 6. CO gas inlet 7. CO gas inlet (CO backup system) 8. N gas inlet (for suppressed O control) 9. N gas inlet (for suppressed O control - N backup system)
  • Page 14 CelCulture...

  • Page 15: General Requirements

    – 2.1 General Requirement 2.1.1 Location Requirements The CelCulture CO Incubator can be placed on the floor or working surface or floor stand. Do not place equipment close to flammable materials or devices that produce excess heat. Before moving or relocating the unit, make sure to: Remove the water in the humidity to avoid spillage.

  • Page 16: Power Requirements

    2.1.3 Power Requirements The equipment is designed to work with an electrical supply of 220 VAC – 240 VAC, 50/60 Hz or 110VAC – 130 VAC, 50/60 Hz. If your available electrical supply is not within these parameters, then a suitable power supply must be used, otherwise damage may be caused to the device or a hazardous situation may result.

  • Page 17: Shelves System Installation

    2.2.4 Shelves System Installation Pilasters are held against the chamber walls using thumbscrews. There are 2 pilaster models – the shelf railing holders in the front pilasters (2) is open upward, while the one in the back pilasters (1) are open toward the back of the unit. Shelf railings (3) are installed by sliding them in the shelf railing holders in the back pilaster, then the front.

  • Page 18: Preliminary Cleaning

    If high humidity level is required, the filtered air exchange can be removed and “Elevated Humidity” can be activated in the control panel. (Call Esco or your distributor to activate this function) 2.6 First Run After the incubator has been properly installed, and connected to the power supply, humidity pan filled, and the unit connected to gas supply, follow the procedures for the unit’s initial start-up.

  • Page 19: Disclaimer

    Set %O2 Set Point. Use UP/DOWN buttons to select the %O2 set point. Press SET to confirm. (For suppressed O model only) 4. Incubator set-up is finish. 5. The unit will run a memory check. 6. Incubator will do initial start-up and will continue to the main menu. The outer door has a magnetic closure, which can be opened by pulling on the rim of the door.
  • Page 20 CelCulture...

  • Page 21: Celculture Control System

    3.1 CelCulture Control System DECON LED HEAT LED C/%RH Display MENU Button Main LCD UP Button ALARM LED DECON Button INJECT LED Display SET Button DOWN Button MUTE Button DECON Button & DECON LED To activate 90 C moist heat decontamination procedure During decontamination procedure, the yellow DECON LED will light up MENU Button When the main LCD shows normal display –...

  • Page 22: Menu Options

    3.2 Menu Options SET TEMP SETTINGS SET %CO2 TEMPERATURE SENSOR SET %O2 %CO2 SENSOR CALIBRATION %O2 SENSOR OVER TEMP ALARM %RH SENSOR LOW TEMP ALARM HIGH %CO2 ALARM LOW %CO2 ALARM NEW CO2 TANK HIGH %O2 ALARM LOW %O2 ALARM LOW %RH ALARM NEW PIN AUDIBLE ALARM...

  • Page 23: Settings

    3.2.1 Settings 3.2.1.1 Set Temperature Your Incubator has an operating temperature range of ambient +3°C to 60°C. The default temperature set point is 37 MENU SETTINGS SET TEMP 20 < X < 60 3.2.1.2 Set %CO2 Your Incubator has a range of 0% -20% CO₂. The default CO₂ set point is 5%. MENU SETTINGS SET %CO2...
  • Page 24 3.2.3.6 High %O2 Alarm (for suppressed O model) The unit will display/sound the alarm when the O level goes above the high %O2 alarm value. The default high %O2 alarm is %O2 set point + 1%. MENU ADMIN HIGH %O2 ALARM SP + 1 ≤...

  • Page 25: Datalogging

    3.2.3.14 Set ULPA Reminder The ULPA Reminder will alert the user to replace ULPA filter at a set period. Default is set to 12 months. MENU ADMIN SET ULPA REMINDER 0 ≤ X ≤ 12 3.2.3.15 Replace ULPA Filter The replace ULPA filter settings must be set when replacing ULPA filter in order for the Set ULPA Reminder to reset based on the set period.

  • Page 26: Service

    3.2.4.2 Data Log Period The Data Log Period allows the user to specify the period in between the system log the data. The period can be set from 1 minute up to 24 hours. MENU DATALOGGING DATA LOG PERIOD 1 MINUTES ≤ X ≤ 24 HOURS 3.2.5 Service 3.2.5.1 Diagnostic Readout This menu will show all parameter and set points that is currently being set or operating in the incubator.

  • Page 27: Analog Output Calibration

    3.3.2 Analog Output Calibration (for units with optional analog output) A set of relay contacts are provided at the rear of the incubator that allows that allow the incubator to output analog signals representing the temperature, %CO2, %O2 (for suppressed O model) and %RH depending on the options available in your incubator.

  • Page 28: Diagnostic Readout

    3.4 Diagnostic Readout Messages Description MAIN_BOARD Main board firmware version. DISP_BOARD Display board firmware version. TEMP READ Snap shot of actual temperature read. ADC TEMP Snap shot of actual Analog to Digital Conversion value of temperature read. TEMP OFFSET Offset value applied for temperature reading on 7 segment display. TEMP SP Temperature set point.

  • Page 29: Alarm

    3.5 Alarm The CelCulture CO Incubator alarm system is shown in the table below. When an alarm is active, the error message appears in the display of the controller. Pressing MUTE button will disable audible alarm for the ring back period. However, the visual alarm continues until the incubator returns to a normal condition.
  • Page 30 CelCulture...
  • Page 31 4.1 Scheduled Maintenance Proper and timely maintenance is crucial for trouble free functioning of any device and your CO incubator is no exception to this rule. We strongly recommend that you follow the maintenance schedule suggested hereunder in order to obtain optimal performance from your incubator. Maintenance to be carried out every Description of Task to Perform Week...
  • Page 32 4.2 Maintenance/Service Log It is good practice (and in some cases regulatory requirement) to maintain a log of all maintenance work carried out on your incubator. CelCulture...
  • Page 33 5.1 Cleaning Procedure 1. Transfer all samples to another CO₂ incubator or store them in a safe place. 2. Turn off and unplug the device. If needed; mark that the unit is deactivated or being serviced. 3. Strip the unit for cleaning: Remove the ULPA filter.
  • Page 34 IR Sensor Removal IR sensor is located behind the blower in the top duct. To remove the IR sensor: 1. Unclip the sensor out of its place 2. Disconnect the probe (1) from its cable (2) Do not touch this part (slotted filter cap) of the sensor TC and O Sensor Removal TC and O...
  • Page 35 Under no circumstances should the unit be disassembled, repaired, or converted by unqualified people. Failure to follow these instructions may cause personal injury and/or loss of property due to malfunctions, electric shocks or fire. Should the unit malfunction, turn off the incubator and disconnect the power supply. Continuing to operate the unit when it shows signs of malfunctioning may cause electric shocks or fire.
  • Page 36 Problem 3: Temperature is above or below temperature set-point Possible Cause Corrective Actions Temperature set-point was Check temperature set-point. Allow 2 hours for the unit to stabilize after recently lowered or raised. adjustment of new set-point. Unit is still not stabilized after If the temperature was lowered, open the outer and inner doors.
  • Page 37 Problem 9: Condensation occurs on the surface of inner door after decontamination process. Possible Cause Corrective Actions Sampling port on inner door is Ensure the sampling port is capped. not installed or capped. Leakage through magnetic Check to make sure outer door can close fully and no gap. gasket on outer door.
  • Page 38 CelCulture...
  • Page 39 APPENDIX...
  • Page 41 Incubator : _______________________________________________________________ Serial Number : _______________________________________________________________ Person in Charge : _______________________________________________________________ 1. This log record should be used by the operator to record any new agent that has been introduced to the incubator during its operation, problems encountered, etc. 2.

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