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HemoCue 201 DM Tutorial

Hemoglobin analyzer
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Hemocue Hb
201 DM
Hemoglobin Analyzer
* READ POLICY PRIOR TO
STARTING TUTORIAL

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Summary of Contents for HemoCue 201 DM

  • Page 1 Hemocue Hb 201 DM Hemoglobin Analyzer * READ POLICY PRIOR TO STARTING TUTORIAL...
  • Page 2 Before Testing a Patient  ORIENT YOURSELF TO YOUR WORKING AREA – Locate testing meter & docking station – Locate testing supplies • Microcuvettes • Liquid Quality Control solutions • Lancets • Alcohol swabs • 2x2 gauze • Gloves • Biohazard container •...
  • Page 3 -Ten microliters of blood are blood at the point of care. drawn into the optical eye by capillary action and  -The HemoCue Hb 201 DM spontaneously mixed with the system consists of a reagents. photometer analyzer with specially designed microcuvettes containing dry ...
  • Page 4  Upon completion of the Initial Orientation and Training and Competency Exam, approved staff will be issued a barcode to be able to use the HemoCue Analyzer.  This barcode in embedded with unique identifiers specific to each staff member.
  • Page 5 Equipment, Reagents and Materials • Analyzer • Docking station Keep meter in docking station when not in use. • Microcuvettes • Liquid Quality control: Level 1 (Lo) and Level 3 (Hi)
  • Page 6 Equipment , Reagents and materials • Alcohol swab • Lancet • 2x2 sterile gauze • Gloves...
  • Page 7 Equipment, Reagents and Materials  Biohazard container  70% isopropanol or mild soap solution for cleaning the analyzer and laser scan window  Refrigerator for Liquid Quality Control storage  Daily Temperature Log (room and refrigerator)  Daily Maintenance Log...
  • Page 8 HemoCue Hb 201 Microcuvettes  Microcuvettes are stored at room temperature away from any direct heat source.  Microcuvettes are moisture sensitive. Always keep the container properly closed.  Unopened containers of microcuvettes may be used until the expiration date listed on bottle.
  • Page 9 HemoCue Liquid Quality Control  Two Levels: Level 1 (Low control) & Level 3 (High control).  An open vial is stable for 30 days when stored at room temperature: 15–  Or is also stable for 30 days when stored in the refrigerator at 2–8...
  • Page 10 Specimen  Capillary whole blood (obtained by fingerstick) required and must be tested immediately.  Specimen labeling is not required when testing is performed in the presence of the patient and only the sample from one patient is tested at a time. ...
  • Page 11 Quality Control Testing Internal Electronic Quality Control (EQC) – The HemoCue Hb 201 DM analyzer has an internal Electronic Quality Control performed when analyzer displays “Selftesting…” – Every time the analyzer is turned on, it will automatically verify the performance of the optronic unit during the “Selftesting”...

  • Page 12: Quality Control Testing

    Quality Control Testing  Liquid Quality Controls (LQC) – Quality Control tests should be performed and passed every day prior to patient testing. (The analyzer will lock down at 0300 and will remain locked until QC is performed.) – Push black button to turn on. –...
  • Page 13 Quality Control Testing • Click level 1 test. • Observe universal precautions. • Wear gloves. • Take one microcuvette out of the vial. • Mix the liquid QC thoroughly by gently rolling the vial between your palms. • Touch the tip of the microcuvette to the hanging drop from the solution vial.
  • Page 14 Quality Control Testing • Gently wipe the edges of the microcuvette on the gauze - avoid touching the opening. • Examine microcuvette for bubbles. • Place the filled microcuvette in the cuvette holder. • Push the cuvette holder into the measuring position (closed).
  • Page 15 Quality Control Testing • Scan the liquid QC barcode. • Scan microcuvette vial. • Analyzer will beep and display a numeric result. • If the result are acceptable press ok. • You may add predefined comments or add your own if desired. •...
  • Page 16 Quality Control Testing • Pull the cuvettle holder out to the “loading” position. • Dispose microcuvette in the biohazard waste container. • Repeat above procedure for Level 3 QC.

  • Page 17: Patient Testing

    Patient Testing • Using two patient identifiers (patient’s full name and date of birth), verify patient identification, and explain the procedure to patient and/or family. • Observe universal precautions. • Turn on the analyzer.
  • Page 18 Patient Testing • Scan Operator Bar Code ID. • Run Liquid Quality Control if it has not been performed. • To run a patient test, press the Microcuvette symbol.
  • Page 19 Patient Testing • The analyzer will display “Enter patient ID”. Click on the icon to scan the patient ID. • Scan the patient’s ID band (barcode) or manually enter the patient’s MRN. • The analyzer will display “Please Fill and Insert a Cuvette”.
  • Page 20 Patient Testing  Obtain patient sample by fingerstick: – After cleaning the patient’s finger with alcohol swab and letting dry use a lancet to prick it. – Wipe away the first 2 or 3 drops of blood with a lint free wipe (gauze). Do not use cotton balls! –...
  • Page 21 Patient Testing • Gently wipe the outside of the microcuvette with gauze using a sideways motion. Do not touch the opening of the microcuvette. • Examine the microcuvette for bubbles. If bubbles are present, use a new microcuvette and repeat sampling. •...
  • Page 22 Patient Testing • The analyzer will beep and the patient test result will be displayed. • Read and record (document) result. • If results are out of the normal range, repeat the test. • Values >23.5 g/dL must be confirmed by sending a STAT patient specimen to the Clinical Laboratory.
  • Page 23 Limitations • Air bubbles in the microcuvette will result in erroneously low values. The microcuvette should be inspected for bubbles before testing. • The microcuvette should be filled in a continuous process. It should never be topped off after the initial filling.
  • Page 24 Liquid QC contain human red cells. Use Universal Precaution when handling the solutions. • Excessive squeezing of the finger can dilute the sample with tissue fluid / interstitial fluid, and may give lower results. MSDS – Located on the SFGH-POCT.org website under “HemoCue”.
  • Page 25 For Further Questions or Information Contact POCT Services SFGH-POCT.org...