Biotek PowerWave Operator's Manual page 154

MULTIPLE WAVELENGTH READS
To cause a read to return data for each indicated wavelength (rather than performing
dual wavelength subtracts or blanking), use the following procedure:
a) Set both wavelengths in the standard reader definition table (see 'V'
command) to '000'. In this case, the single/dual wavelength select parameter
(byte #30, bit #6) will be ignored.
b) Starting at byte #67 in the same table, replace blanking map values with six
wavelength's worth of ASCII characters (3 bytes each, 24 bytes total - if
wavelength not to be used, set to '000').
c) Send reader definition table to reader. ('V' command).
d) If reading a range of selected strip(s), send Select Strips command ('%') as
defined above.
e) Send Read command ('S') to perform the actual read.
Response data from the read will consist of an entire 8 x 12 plate for each indicated
wavelength, or an 8 x <#strips> plate consisting of strip(s) selected by the Select
Strips command ('%').
Wavelengths indicated by '000' will return no data.
NOTE: Data returned for each plate read at an indicated wavelength will start with
<CR> followed by that wavelength's well ODs as defined in the 312
protocol spec. Each plate will be terminated with a <^Z> character
followed by a checksum. Checksum calculation starts with the first byte
AFTER the data start code (<CR>), up through and including the <^Z>
data terminator code. The checksum is transmitted as a single hex data
byte.
B-18 Computer Control