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Summary of Contents for IRIS Cytofuge 12
- Page 1 Cytofuge 12 Cytocentrifuge System ® Operator’s Manual A Division of IRIS International, Inc.
- Page 2 Operator’s Manual Cytofuge 12 Cytocentrifuge System ® Model Number M802 For in-vitro diagnostic use Product Number CF12 – Cytofuge 12 for 100-240 VAC, 50/60 Hz StatSpin and Iris are registered trademarks of IRIS International, Inc. Copyright 2009 Printed in U.S.A.
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Page 3: Table Of Contents
Checking/Replacing Rotor Seal ..............11 Checking/Replacing Drive Cone O-rings ............11 Checking the Rotor Speed ................12 Troubleshooting .................... 12 Service ......................13 Section 6 ......................14 Specifications ....................14 References ....................15 Iris Sample Processing Warranty ..............16 Limitation of Liability ..................16... -
Page 4: How To Use This Manual
CAUTION - The cover interlock by-pass is for emergency use only. Do not attempt to operate the Cytofuge 12 while the latch release tool is inserted in the housing. Misuse of the interlock bypass may expose the operator to unsafe conditions. - Page 5 CAUTION – Do not lean, or place materials, on the cytocentrifuge. CAUTION - Follow Universal Precautions with all biological specimens, regardless of whether the specimen is known to contain an infectious agent. Bioseals cannot be relied upon as a sole safeguard against contamination by pathogens.
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Page 6: Section 1
Section Unpacking and Installation Inspect Packaging ® Cytofuge 12 Cytocentrifuge and its accessories are delivered in a single carton. If the cytocentrifuge or accessories have been damaged in transport, please inform your carrier immediately. NOTE: Save the shipping carton and packing materials to facilitate return should service be required. Verify Contents The package contains: ®... -
Page 7: Connect Power
Turn on the power switch (located on the rear panel, above the line cord connector). When the Cytofuge 12 completes the power-on check it will give a two-beep ready signal. Press the STOP/OPEN button to release the latch and open cover. -
Page 8: Section 2
Speeds from 200 rpm to 2000 rpm and cycle times from 1 minute to 99 minutes may be selected. The system features three acceleration rates and dynamic braking to protect delicate samples. Symbols and Definitions Indicates the Iris Sample Processing Product/Reference Number product/catalog number... -
Page 9: Operator Controls
Operator Controls Start Initiates the selected cycle Interrupts the cycle and releases the cover when the rotor has stopped. Stop/Open Cancels Programming Mode Enters Programming Mode to store speed, time and Save acceleration settings Depress to select acceleration profile - Low, Medium or Acceleration Select High Increments program, speed or time... -
Page 10: Error Indicators
Filter Concentrators , Disposable; Box of 200 Filter Cards, 192 Concentrators FFR1 Filter Concentrators, Reusable; Box of 200 Filter Cards, 20 Concentrators FFCL Stainless Steel Clips for Filter Concentrators; Box of 4 CFRT-12 Cytofuge 12 Rotor Assembly, replacement or spare, includes cover CFRC-12 Cytofuge 12 Rotor Lid, replacement... -
Page 11: Section 3
The approximate cell concentration of the specimen should be established prior to slide preparation on the Cytofuge 12. Samples containing higher than optimal cell concentration will produce slides with cells too closely packed or overlapping. Samples containing too low a cell concentration will yield slides where cells are difficult to find, count, or examine. -
Page 12: Section 4
Cytofuge 12 into the small hole located in the front of the housing. Push the tool inward to release the latch. Remove the tool before re-starting the cytocentrifuge. -
Page 13: Spinning Samples
Programming - pg 8). The table below lists the relative centrifugal force (RCF) at several speeds. Press start to begin cycle. The Cytofuge 12 will spin for the set time and automatically stop. Note: Settings cannot be changed once the cycle begins. -
Page 14: Programming
Cytofuge 12 operating parameters generally depend upon the size and specific gravity of the cells to be concentrated onto the microscope slide. The operator should experiment with different settings to achieve optimum performance for specific applications. The following are general guidelines:... -
Page 15: Section 5
Rinse the parts under running tap water or soak for 5 minutes. Dry the parts with a clean absorbent paper towel or allow to air dry. Replace the rotor seal, re-attach the lid and re-install the rotor in the Cytofuge 12. Rotor Sterilization If necessary, remove rotor from the cytocentrifuge. -
Page 16: Checking/Replacing Rotor Seal
Checking/Replacing Rotor Seal Over time seals may become brittle and develop cracks. To ensure optimal rotor performance, check the inner and outer seals of the rotor regularly for any tears or cracks. The center seal is user replaceable. The outer seal is part of the rotor bowl – if this seal becomes worn or damaged a new rotor bowl should be purchased. -
Page 17: Checking The Rotor Speed
Checking the Rotor Speed Rotor speeds ( 5%) may be checked with a stroboscope or photoelectric tachometer. Remove the rotor and locate the reflective patch located on the top of the drive cone. Close the instrument cover. Set the instrument to the desired speed. Point the tachometer through the transparent cover at the reflective patch. -
Page 18: Service
Service Refer all service to Iris Sample Processing or an Authorized Repair Facility. Instrument fuse(s) are located on the rear panel. Remove the line cord to access the fuse holder. Replace with fuses of the same type and rating. 2.5A 250V F 5 x 20 mm Be sure to complete the Warranty Registration Card as directed. -
Page 19: Section 6
Section Appendix Specifications ® Product Name Cytofuge 12 Cytocentrifuge Product No. CF12 Model No. M802 Capacity 12 Filter and/or Cell concentrators Programs 24 user configurable Range 200-2000 rpm Speed Accuracy ± 5% Range 1-99 minutes Time Accuracy ± 2 seconds Range Acceleration LO (45-50 RPM/sec);... -
Page 20: References
CDC. Updated: US Public Health Service Guidelines for the Management of Occupational Exposures to HBV, HCV and HIV and Recommendations for Post Exposure Prophylaxis. Appendix A and B. MMWR 50 (RR-11): 1-42, June 29, 2001. A Division of IRIS International, Inc. Westwood, MA 02090 USA Tel: (781) 551-0100 Fax: (781)551-0036 www.proiris.com... -
Page 21: Iris Sample Processing Warranty
The purchaser shall be deemed liable for any and all claims, losses, or damages incurred by the use or misuse of the Iris Sample Processing instrument by the purchaser, its employees or others, following receipt of the...
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