Toyota 2006 Highlander Owner's Manual page 350

To detach the spare tire:
1. Vehicles with aluminium wheels—
Remove the inside spare tire cov-
er by turning it counterclockwise.
340
'06 HIGHLANDER_U (L/O 0508)
2. Pull the strap and open the center
deck board.
3. Vehicles with third seat—
If the third seat is upright, hang
the center deck board on the
right−side head restraint.