Zeiss KF 2 ICS Operating Manual
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Zeiss KF 2 ICS Operating Manual

Transmitted light microscope
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KF 2
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Transmitted Light Microscope
Operating Manual

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Summary of Contents for Zeiss KF 2 ICS

  • Page 1 KF 2 Transmitted Light Microscope Operating Manual...
  • Page 2 Carl Zeiss KF 2 Knowledge of this manual is required for the operation of the instrument. Would you therefore please make yourself familiar with the contents of this manual and pay special attention to hints concerning the safe operation of the instrument.

  • Page 3: Table Of Contents

    KF 2 Carl Zeiss CONTENTS Page INTRODUCTION ......................... I Title page ..........................I Copyright ........................... II Contents ..........................III Notes on instrument safety ....................IV Notes on warranty ......................V Overall view........................VI DESCRIPTION ......................... 1-3 Terms, intended use ......................1-3 Instrument description ..................... 1-3 Modules ..........................

  • Page 4: Notes On Instrument Safety

    If it is established that the protection measures are no longer effective, the instrument must be switched off and safeguarded against inadvertent operation. For the repair of the KF 2 microscope, please contact the Carl Zeiss Microscopy Service in Germany (see page 4-6) or your local Carl Zeiss agency.

  • Page 5: Notes On Warranty

    All repairs inside the instrument may only be performed by the Carl Zeiss Microscopy Service in Germany (see page 4-6) or your local Carl Zeiss agency.

  • Page 6: Overall View

    Carl Zeiss KF 2 Overall view of the KF 2 microscope B 40-028 e 10/97...
  • Page 7 KF 2 Carl Zeiss DESCRIPTION Contents DESCRIPTION .........................1-3 Terms, intended use ......................1-3 Instrument description .....................1-3 Modules ..........................1-4 Technical data .........................1-6 List of illustrations Fig. 1-1 KF 2 microscope equipment..................1-4 B 40-028 e 10/97...
  • Page 8 Carl Zeiss KF 2 B 40-028 e 10/97...

  • Page 9: Terms, Intended Use

    KF 2 Carl Zeiss DESCRIPTION Terms, intended use Manufacturer's name: KF 2 microscope The KF 2 microscope is a universal microscope for the visualization of fine structures and forms in biology and medicine. These are typical application fields of this microscope:...

  • Page 10: Modules

    Carl Zeiss KF 2 Modules Fig. 1-1 KF 2 microscope equipment B 40-028 e 10/97...
  • Page 11 KF 2 Carl Zeiss KF 2 microscope equipment Cat. No. KF 2 microscope stand with 4-position W 0.8 nosepiece, condenser 0.9 AS with aperture diaphragm and additional lens for low-power observation, built-in illuminator, stabilized power supply, power cable with safety plug or American flat plug, 2 halogen lamps 6V/10W and instrument protection cover.

  • Page 12: Technical Data

    Carl Zeiss KF 2 Technical Data Dimensions Dimensions (width depth height) ..............185 400 mm Weight Stand equipment......................... approx. 3 kg Binocular tube 45°/20 ICS....................approx. 1 kg KF 2 microscope, complete ..................... approx. 5 kg Ambient conditions Storage and transport (in packaging): Permissible ambient temperature ..................

  • Page 13: Start-Up

    KF 2 Carl Zeiss START-UP Contents START-UP........................2-3 Installation of the instrument ...................2-3 List of illustrations Fig. 2-1 Attachment of binocular tube ..................2-3 Fig. 2-2 Positions of binocular tube....................2-3 Fig. 2-3 Screwing in objectives......................2-4 Fig. 2-4 Attachment of eyepieces ....................2-4 Fig. 2-5 Switch on line operation mode ..................2-5...
  • Page 14 Carl Zeiss KF 2 B 40-028 e 10/97...

  • Page 15: Installation Of The Instrument

    KF 2 Carl Zeiss START-UP Installation of the instrument The KF 2 microscope, including accessories, is delivered in standard packaging. We would recommend you to keep the packaging material so that the instrument can be stored for a longer period of time or returned to the manufacturer.

  • Page 16: Fig

    Carl Zeiss KF 2 Screwing in objectives Remove dust caps (2-2/2) according to the number of objectives and screw objectives (2-2/3) in nosepiece clockwise one by one, starting with the lowest magnification. Nosepiece eyes which required should be closed with dust caps.

  • Page 17: Fig

    View the type label (4-2/2) to check whether the built-in lamp supply complies with the present line voltage. Should the line voltage not lie within the range of the built-in lamp supply, please get in touch with the nearest Carl Zeiss agency. Switch on line operation Connect instrument to the power plug via the power cable.

  • Page 18: Fig

    Carl Zeiss KF 2 Switch on the line/battery operation Fig. 2-5 Switching on the line/battery operation Line operation Connect KF 2 to line plug via line cable. Set switch (2-6/4) to "Mains ON". Set the required brightness using the "Dimmer Mains" control (2-6/2).

  • Page 19: Operation

    KF 2 Carl Zeiss OPERATION Contents OPERATION ........................3-3 Objective selection......................3-3 Visual overall magnification....................3-4 Eyepiece reticles.......................3-4 Setting the microscope in brightfield ................3-6 Setting the microscope for phase contrast ................3-8 Setting the microscope in darkfield ................3-10 Photomicrography ......................3-11 Videomicroscopy ......................3-12 Illumination via mirror and condenser 0.9 AS..............
  • Page 20 Carl Zeiss KF 2 B 40-028 e 10/97...

  • Page 21: Operation

    KF 2 Carl Zeiss OPERATION Choice of objectives The objectives are the centerpiece of the microscope. The following is an example of how objectives can be labeled: CP-"Achromat" 10 /0.25 where objective magnification 0.25 numerical aperture infinite tube length usable for covered and uncovered specimens 0.17...

  • Page 22: Visual Overall Magnification

    Carl Zeiss KF 2 Visual overall magnification The M visual overall magnification of the microscope results from the multiplication of the M objective magnification with the M eyepiece magnification: eyep. Example: eyep. 10 = 400 Eyepiece reticles The 40 objective mentioned in the example under 3.2 produces an intermediate image of the object with the magnification 40 : 1.
  • Page 23 KF 2 Carl Zeiss Compensating for myopia Two focusing eyepieces PL 10 /18 Br. foc. are required to allow the user to compensate for differences in the visual performance of his two eyes. Use the eyelens of the focusing eyepiece to focus on the line figure of the eyepiece reticle; focus on the edge of the field of view if no eyepiece reticle is used.

  • Page 24: Setting The Microscope In Brightfield

    Carl Zeiss KF 2 Setting the microscope in brightfield Place specimen on the sample stage with the cover slip pointing upwards. The condenser 0.9 AS (3-2/3) is aligned with the specimen stage and firmly attached to it. It contains an aperture diaphragm to control the image contrast.
  • Page 25 KF 2 Carl Zeiss Look through the fixed eyepiece of the binocular tube first and focus on the object via the focusing drive (3-3/2). Then set the focus for the other eye by turning the eyelens of the focusing eyepiece (3-3/1). Set the distance of the tubes in such a way that you can see a sharply limited field of view with both eyes.

  • Page 26: Setting The Microscope For Phase Contrast

    Carl Zeiss KF 2 Setting the microscope for phase contrast The condenser lever (3-2/1) can be exchanged for a condenser lever with phase stop Ph 2 (46 52 14) (3-4/3). For this, unscrew the knurled knob (3-2/4), pull out the changer in downward direction, slide in the new changer until stop and fix it via the knurled knob.

  • Page 27: Fig

    KF 2 Carl Zeiss Turn the screws (3-4/2) to superimpose the dark Ph ring on the bright ring. The dark phase ring of the objective must fully cover the bright illumination ring. A centering telescope (3-5/1) can also be inserted into the tube instead of a diopter. If a centering telescope is used, its eyelens must be shifted until the illuminating ring is in focus.

  • Page 28: Setting The Microscope In Darkfield

    Carl Zeiss KF 2 Setting the microscope in darkfield The condenser lever (3-2/1) can be exchanged for a condenser lever with darkfield stop D 0.7) (3-7/2). For this, unscrew the knurled knob (3-2/4), pull out the changer in downward direction, slide in the new changer until stop and fix it via the knurled knob.

  • Page 29: Photomicrography

    KF 2 Carl Zeiss Photomicrography The KF 2 microscope (with phototube) can be changed from observation to photography via the pushrod (3-8/7). Special connectors and the T 2 adapter allow commercially available 35 mm SLR cameras and special microscope cameras (e.g. MC 80 ) to be attached to the camera port of the KF 2 .

  • Page 30: Videomicroscopy

    Carl Zeiss KF 2 Videomicroscopy Various TV 60 adapters allow 1-chip black-and-white and color cameras to be attached to the KF 2 with phototube. Illumination via mirror and condenser 0.9 AS Align (turn and tilt) the flatfield surface of the...

  • Page 31: Care, Maintenance And Troubleshooting

    KF 2 Carl Zeiss CARE, MAINTENANCE AND TROUBLESHOOTING Contents CARE, MAINTENANCE AND TROUBLESHOOTING ............4-3 Care and maintenance of the instrument .................4-3 Troubleshooting and requesting service................4-4 List of illustrations Fig. 4-1 Changing the fuse for line/battery operation ..............4-4 Fig. 4-2 Changing the halogen lamp.....................4-5 Fig.
  • Page 32 Carl Zeiss KF 2 B 40-028 e 10/97...

  • Page 33: Care And Maintenance Of The Instrument

    KF 2 Carl Zeiss CARE, MAINTENANCE AND TROUBLESHOOTING Care and maintenance of the instrument Maintenance of the KF 2 is limited to the following operations: Cover the instrument with the dust cover after every use. Do not install instrument in a damp room.

  • Page 34: Troubleshooting And Requesting Service

    Carl Zeiss KF 2 Troubleshooting and requesting service Troubleshooting on the KF 2 is limited to only a few actions: Checking the line voltage Changing the fuses for line/battery operation Change and adjustment of the 6 V / 10 W halogen lamp.

  • Page 35: Changing The Halogen Lamp

    KF 2 Carl Zeiss Change and adjustment of the 6 V / 10 W halogen lamp. Place the microscope on its side on the table. Loosen the knurled screw (4-2/1) and pull out the illuminator (4-2/4). Remove the halogen lamp (4-3/1) from the metal clamps (4-3/2).
  • Page 36 Carl Zeiss. In the case of subsequent orders or when service is required, please get in touch with the Carl Zeiss Microscopy Service in Germany or your local Carl Zeiss agency.

  • Page 37: Annex

    KF 2 Carl Zeiss ANNEX List of abbreviations ........................A-3 Physical and technical units ......................A-3 EC conformity declaration ......................A-5 Certification in accordance with DIN EN ISO 9001 / DIN EN 46001 ..........A-7 B 40-028 e 10/97...
  • Page 38 Carl Zeiss KF 2 B 40-028 e 10/97...

  • Page 39: List Of Abbreviations

    KF 2 Carl Zeiss List of abbreviations aspheric for eyeglass wearers Charge-Coupled Device Canadian Standards Association diameter direct current German industrial standards coding system for the storage of electronically legible information, e.g. film speed European Economic Community European standards free working distance foc.
  • Page 40 Carl Zeiss KF 2 B 40-028 e 10/97...

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