Agilent Technologies 7697A Advanced Operation Manual page 44

3
Method Development
44
• The HS will start the method, running one vial at a
time, until it would exceed the specified upper limit on
the parameter.
• View the current method parameters by pressing
[Temp], [Time], or [Vial]. As the HS increments the
method parameter for each new vial, the new value is
displayed.
Oven temperature
When incrementing oven temperature, consider the following:
• Higher temperatures generally improve peak areas.
• Do not exceed the solvent (or analyte) boiling point.
• Incrementing temperature can increase throughput.
• All thermal zones increment at the same rate. If a heated
zone reaches (or would exceed) its maximum temperature,
it will hold at its maximum temperature for any
remaining vials. For example, consider an oven
temperature of 250 °C with a transfer line temperature of
275 °C. If incrementing 10 °C, on the third run, transfer
line temperature would be 305 °C while the oven would
be at 280 °C. The maximum temperature of the transfer
line would be exceeded. Instead, the temperature holds at
300 °C. See the example in
Table 10 Example temperatures, in °C, during parameter increment of
10°C per step
Oven
250
260
270
280
290
300
• Vials in this case are run in series. There is no overlap
since the oven temperature differs for each vial.
• Do not enter a series that exceeds the number of
available vials in the tray (or carousel).
Table 10
Transfer line
275
285
295
300
300
300
below.
Sample loop
265
275
285
295
300
300
Advanced Operation