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Kit Components
Table 1
DNA 1000 Kit
Agilent DNA 1000 Kit (reorder number 5067-1504)
DNA Chips
25 DNA Chips
1 Electrode Cleaner
Syringe Kit
1 Syringe
3 Spin Filters
1
"This product is provided under a license by Life Technologies Corporation to Agilent Technologies. The purchase of this
product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the
product only as described in accompanying product literature. The sale of this product is expressly conditioned on the buyer
not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliat-
ed third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell or otherwise transfer
this product or its components to any third party, or use for any use other than use in the subfields of research and develop-
ment, quality control, forensics, environmental analysis, biodefense or food safety testing. For information on purchasing a
license to this product for purposes other than described above contact Life Technologies Corporation, Cell Analysis Busi-
ness Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354."
Assay Principles
Agilent DNA kits contain chips and reagents designed for sizing and analysis of DNA fragments. Each Agilent
DNA kit contains an interconnected set of microchannels that is used for separation of nucleic acid
fragments based on their size as they are driven through it electrophoretically. Agilent DNA kits are designed
for use with the Agilent 2100 bioanalyzer only.
Assay Kits
The Agilent DNA 1000 kit provides higher resolution of smaller fragments in comparison of our other DNA
sizing kits. It can be used for the following applications:
• Analysis of PCR and RT-PCR products
• RFLP analyses
• Heteroduplex analysis using mismatch cleavage enzymes
• QC of sequencing templates
The complete DNA 1000 kit guide can be found in the online help of the 2100 Expert Software.
Agilent DNA 1000 Kit
Quick Start Guide
DNA 1000 Reagents (reorder number 5067-1505)
(yellow) DNA Ladder
(green) DNA Markers (2 vials)
(blue) DNA Dye Concentrate
(red) DNA Gel Matrix (3 vials)
1
(1 vial)
Agilent Technologies
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Related Manuals for Agilent Technologies DNA 1000 Kit
Summary of Contents for Agilent Technologies DNA 1000 Kit
- Page 1 • Analysis of PCR and RT-PCR products • RFLP analyses • Heteroduplex analysis using mismatch cleavage enzymes • QC of sequencing templates The complete DNA 1000 kit guide can be found in the online help of the 2100 Expert Software. Agilent Technologies...
- Page 2 Agilent DNA 1000 Kit Quick Start Guide Storage Conditions • Keep all reagents and reagent mixes refrigerated at 4 °C when not in use to avoid poor results caused by reagent decomposition. • Protect dye and dye mixtures from light. Remove light covers only when pipetting. Dye decomposes when exposed to light.
- Page 3 Agilent DNA 1000 Kit Quick Start Guide Setting up the Chip Priming Station Replace the syringe: Unscrew the old syringe from the lid of the chip priming station. Release the old syringe from the clip. Discard the old syringe. Remove the plastic cap of the new syringe and insert it into the clip.
- Page 4 Agilent DNA 1000 Kit Quick Start Guide Preparing the Gel-Dye Mix Allow DNA dye concentrate (blue ) and DNA gel matrix (red ) to equilibrate to room temperature for 30 min. Add 25 μL of DNA dye concentrate (blue ) to a DNA gel matrix vial (red ).