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TomTec QUADRA 4 Automated Liquid Handling Manuals
Manuals and User Guides for TomTec QUADRA 4 Automated Liquid Handling. We have
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TomTec QUADRA 4 Automated Liquid Handling manual available for free PDF download: Operator's Manual
TomTec QUADRA 4 Operator's Manual (217 pages)
Brand:
TomTec
| Category:
Laboratory Equipment
| Size: 5 MB
Table of Contents
Table of Contents
2
Preface
9
Unpacking Instructions
10
Locating the Quadra 4
10
Section I - General Information
11
Specifications & Technical Information
12
Warnings, Cautions, Notes, & Symbols
13
Introduction
14
Quadra 4, Front View
15
Quadra 4, Side Views
16
Quadra 4 (with Stackers), Rear View
17
Quadra 4 (Without Stackers)
18
Quadra 4 Access Panel
19
Compressor & Pneumatics
20
Section II - Operational Functionality
21
Pipetting System
21
Quadra 4 General Information
22
Quadra 4 Product Line
23
Section III - Quadra Command System Op Manual
24
Introduction
25
What's New
25
Version 4.8.0
25
Version 4.7.7.30
25
Version 4.7.7.29
25
Major Changes to the Quadra 4 Command Software
26
Drag and Drop Operations
26
Simplified Dialogs
26
Shuttle Labware Labels
27
Labware and Program Function Icon Changes
28
Tip Load Counter
29
PSID2 Mirror Rack Checking
30
Quadra 4 - Getting Started
32
Preparing the Quadra to Run a Program
33
Main Screen
34
Labware - Components Area
35
Shuttle Layout Area
36
Shuttle Position Data Dialog
36
Pump Volume Box
37
Program Operations Box
38
Program Steps Area
39
Menu Bar
40
File Menu
40
Edit Menu
41
System Menu
42
Database Menu
43
Tools Menu
43
Utilities Menu
44
Options Menu
44
View Menu
45
Reports Menu
45
Help Menu
46
Program Operations
48
Alert Timer
48
Arm Plate Transfer Operation
49
Aspirate Sample
50
Autotrap Operations
52
Delay Program
53
Dispense Sample
54
Empty Sample
56
External Device
58
GOSUB Program
59
GOTO Program
60
Load Tips
61
Lockout Shuttle Position
62
Loop
62
Magnetic Nest
63
MIX Sample
64
Move Arm
66
Move Shuttle
67
Move Stage
68
Nitrogen Dry
69
Pause
70
Pressure Control
71
Program Note
71
Quit
72
Restack Plates
72
Serial Dilution
73
Shaker Nest
75
Shuck Tips
77
Shuttle Layout Change
77
SPE Pressure Vacuum Operation
78
SPE Vacuum Box Operation
80
Stacker Operations
81
Target Method
82
Timed Dispense
84
Vacuum Box Operation
85
Vacuum Control
86
Wash
87
Program Operations Common Options
89
Additional Options
89
Dip Tips
90
Select Shuttle Position
90
Stage Operations
91
Touch off
93
Database Editor
94
The Menu Bar
95
The Plate Panel
96
The Fixture Panel
97
The Touch off Panel
98
Importing Plates-Reservoirs-Fixtures
98
Options
99
User Preferences
99
Default Plates
101
Default Reservoirs
102
Configure Model
103
Configure Com Port
104
Set User Defined Output Names
105
Set Arm Position Names and Heights
106
Displayed Fixtures
106
Edit Display and Order of Fixtures
107
Current File Settings Options
108
Logging Options
108
Runtime Database Usage Options
109
View Preferences
110
Utility
111
Shuttle/Stacker
111
Stage
112
Pinch Tubes
114
Pump
115
System
116
Trap Operations
116
Miscellaneous
118
Arm - Vacuum Box
119
Status
120
Pump Calibration Tables
121
Utility User Outputs
124
Tools
125
Tools Serial Dilution Wizard
125
Setup
125
Set Source Row/Column
126
Set Sample Aspirate Volume and Height, Row, and Column Offsets
127
Set Initial Dilution Row/Column
128
Serial Dilution
128
Technical Tips
129
Introduction
129
Pre-Planning
129
Labware
129
Labware Location
129
Writing the Protocol
130
System Parameters
132
System Parameters - Stage
133
System Parameters - Shuttle
134
System Parameters - Pump
135
System Parameters - Miscellaneous
136
System Parameters - System Info
137
System Parameters - Shuttle Alignment Utility
138
System Parameters - View System Parameters
139
Vacuum Calibration
140
File Management
141
File Management - Plate & Fixture Databases
143
File Management - Configuration Database
145
File Management - Curve Database
146
File Management - System Parameters Database
148
Tomtec Filesystem Watcher Service
149
Filesystem Watch Marketing Information
149
System Security
149
File System Monitoring
150
Program Step Logging
150
Why Use CFR11
150
Installation
151
FDA CFR11 Documentation
151
Filesystem Installation Information
154
Overview
154
Installation
154
Configuration
155
Usage
156
OS Requirements
157
Quick Start
158
Providing 21CFR Part 11 Security for the Quadra 4
159
Section IV - Pipettor Heads
163
Quadra 4 Air Displacement Pipettor Heads
163
Quadra 450Μl Disposable Tips
163
Organic to Aqueous Pipetting
163
Non Contact Dispensing
164
Clearing Tip Residual
164
Quadra 450Μl Tip Design
164
Loading the 450Μl Disposable Tips
164
Adjusting Tip Load Switch
165
Quadra 4 Positive Displacement Pipettor Heads
166
Quadra 4 Disposable Multi Volume Head (DMV)
167
Setting the Zero Volume Adjustment for Air Displacement Pipettor Heads
170
Quadra Shuttle
172
Stackers
172
Quadra Stage
173
Section V - Suggested Pipetting Methods & Recommendations
175
Aspirating
175
Pipeline Pipetting
175
Dispensing
176
Mixing
176
Titration
176
Bubbling
177
Injection
177
Section VI - Pipetting with the Positive Displacement Pipettor Head
178
Pipetting 0.5Μl with Positive Displacement
178
Dispensing 0.5Μl Spots - Aqueous
178
Dispensing 0.5Μl Spots - DMSO
179
Protocol Verification
179
Section VII - Bioanalytical Applications
180
Aqueous and Organic Pipetting
180
Protein Precipitation, Liquid/Liquid, and SPE
180
Protocol or Program Security
181
Calibration for the Quadra 4
181
Section VIII - Quadra 4 Accessories
184
Custom Reservoirs
184
Keeping Participate Matter in Suspension
184
Stirring Reservoir
184
Constant Level Reservoir
185
Shaker Nest
185
Live Bottom Reservoir
185
Thermal Retention Reservoir
185
Peltier Nest
186
SPE Vacuum Box
186
Filtration System for the Quadra 4
186
Auto Regulated Mini Vacuum Trap
187
Fully Automated Filtration System
187
Section IX - Pipetting Recommendations
188
Tip# 1
188
Tip #2
188
Tip #3
188
Tip #4
188
Tip #5
189
Section X - Calibration of the Quadra 4 ® 450Μl
190
Equipment Required
191
Weighing Vessels
191
Calibration Curve Procedure
192
Verification of Calibration Curve Procedure
193
Evaporation
193
Pipetting to Weighing Cycle Time
194
Setup Procedure
194
Adding the New Calibration Curve into the Quadra Command System from Data
194
Points
194
Calibration Template - Q4" Spreadsheet
195
Creating a New Calibration Curve with Water
196
Creating a Verification Curve
198
Calibration Template" for the Verification Curve
200
Calibration Instructions for 450 Μl Head
201
Definitions
201
Liquid Used for Calibration Curve Set Volumes
201
Ambient Calibration Conditions
201
Importing an Existing Calibration Curve
201
Section XI - some Properties of Liquids that Affect Pipetting Accuracy
202
The Relationship between Liquid and Gaseous States of a Solvent Have an Effect on Pipetting
202
Additional Properties of Liquids that Might Have an Effect on Pipetting
202
To Compensate for These Properties of Liquids, the Aspirate and Dispense Steps Have Options to Improve Performance
203
Section XII - Head Removal, Installation and Alignment
204
Top Rear View
204
Top Front View (Open Panel)
205
Head Installation
206
450Μl Backfill Head Back View
208
Tip Block Assembly
209
55Μl SV Head
210
SV Tip and Seal Assembly
211
Section XIII - Routine & Preventive Maintenance by Operator
212
Maintenance and Schedule
212
Routine & Preventive Maintenance by Operator
212
Lubrication Kit
212
Cleaning the Pipettor Tips
213
Fuse Replacement
214
Warranty
215
Return Policy
215
Quadra 4 Decontamination Form
216
Tomtec Contact Information - if You Need Help
217
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