TomTec QUADRA 4 Operator's Manual
  1. Manuals
  2. Brands
  3. TomTec Manuals
  4. Laboratory Equipment
  5. QUADRA 4
  6. Operator's manual

TomTec QUADRA 4 Operator's Manual

Hide thumbs
Table of Contents

Advertisement

Quick Links

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the QUADRA 4 and is the answer not in the manual?

Questions and answers

Summary of Contents for TomTec QUADRA 4

  • Page 2: Table Of Contents

    Version 4.8.0 ......................25   Version 4.7.7.30 ....................25   Version 4.7.7.29 ....................25   1.2 Major Changes to the Quadra 4 Command Software ........26   1.2.1 Drag and Drop Operations ................26   1.2.2 Simplified Dialogs ..................26  ...
  • Page 3 3.32 Target Method ....................82   3.33 Timed Dispense .................... 84   3.34 Vacuum Box Operation ................. 85   3.35 Vacuum Control ..................... 86   3.36 Wash ......................87   3.37 Program Operations Common Options ............89   Tomtec Quadra 4 Operator Manual...
  • Page 4 7.1.2 Set Source Row/Column ................126   7.1.3 Set Sample Aspirate Volume and Height, Row, and Column Offsets ..127   7.1.4 Set Initial Dilution Row/Column ..............128   7.1.5 Serial Dilution ..................... 128   Tomtec Quadra 4 Operator Manual...
  • Page 5 11.2.5 OS Requirements ..................157   11.2.6 Quick Start ....................158   11.3 Providing 21CFR Part 11 Security for the Quadra 4 ........159   Section IV – Pipettor Heads ..............163   1.0 Quadra 4 Air Displacement Pipettor Heads ............. 163  ...
  • Page 6 2.2 Adjusting Tip Load Switch ................165   3.0 Quadra 4 Positive Displacement Pipettor Heads ............ 166   4.0 Quadra 4 Disposable Multi Volume Head (DMV) ............ 167   5.0 Setting the Zero Volume Adjustment for Air Displacement Pipettor Heads .... 170  ...
  • Page 7 3.0 Tip #3 ......................188   4.0 Tip #4 ......................188   5.0 Tip #5 ......................189   ® Section X – Calibration of the Quadra 4 450µL ........190   1.0 Equipment Required ....................191   2.0 Weighing Vessels ....................191  ...
  • Page 8   3.0 Fuse Replacement ....................214   WARRANTY ........................215   RETURN POLICY ......................215   QUADRA 4 DECONTAMINATION FORM ..............216   Tomtec Contact Information - If You Need Help ............217   Tomtec Quadra 4 Operator Manual...

  • Page 9: Preface

    This manual provides general information, installation and operation instructions for the TOMTEC QUADRA 4 ® Every effort has been made to avoid errors in text and diagrams, however, TOMTEC assumes no responsibility for any errors or omissions which may appear in this manual.

  • Page 10: Unpacking Instructions

    When selecting a suitable place to set up your Quadra 4, please consider the following: The Quadra 4 should be placed on a level surface. A slanted surface can cause the liquid to pool to one side of a well or reservoir and may impair your ability to fully pipette out small volumes.

  • Page 11: Section I - General Information

    BY THE MANUFACTURER, THE PROTECTION PROVIDED BY THE EQUIPMENT MAY BE IMPAIRED. The Quadra 4 is designed for use with various liquids, aqueous or organic solutions with a pH near neutral. As the pH of the solution moves away from neutral, various parts may be affected, depending on the length of time they are in contact.

  • Page 12: Specifications & Technical Information

    9.5” (241mm) L/R Shuttle Extension: 6” (153mm) (Front to Back) Electrical Specifications Power requirements: 100VAC NOM, 60Hz @ 4.0 AMP 105-125VAC 60Hz @ 4.0 AMP 230-240, 50Hz @ 2.0 AMP Pneumatic Specifications Internal Compressed Air Tomtec Quadra 4 Operator Manual...

  • Page 13: Warnings, Cautions, Notes, & Symbols

    The exclamation point within an equilateral triangle is intended to alert the user to the presence of important operating and maintenance (servicing) instructions in the literature accompanying the instrument. Tomtec Quadra 4 Operator Manual...

  • Page 14: Introduction

    Quadra 4 Command Software, and wireless keyboard. In addition, the embedded PC provides 4 USB ports and internet network capabilities. This manual will describe the standard Quadra 4 Pipettor - both with or without stackers. Additional pipetting head configurations and stage options are outlines in APPENDIX A at the end of this manual.

  • Page 15: Quadra 4, Front View

    Quadra 4 Pictorials Quadra 4, Front View Tomtec Quadra 4 Operator Manual...

  • Page 16: Quadra 4, Side Views

    Quadra 4, Side Views Tomtec Quadra 4 Operator Manual...

  • Page 17: Quadra 4 (With Stackers), Rear View

    Quadra 4 (with stackers), Rear View Aux. Pres sure Tomtec Quadra 4 Operator Manual...

  • Page 18: Quadra 4 (Without Stackers)

    Quadra 4 (without stackers) Tomtec Quadra 4 Operator Manual...

  • Page 19: Quadra 4 Access Panel

    Quadra 4 Access Panel (Front Access Panel) Recommended to be used by TOMTEC Service Technicians only! Tomtec Quadra 4 Operator Manual...

  • Page 20: Compressor & Pneumatics

    This affects the life of the pressure gauge. Blowout Pressure: This is set by the regulator on the right hand side of the Quadra 4. It will range from 0psi to 5psi. A more accurate pressure reading is available on the front display. Elevator Pressure:...

  • Page 21: Section Ii - Operational Functionality

    This, combined with the multiple stations on the Quadra 4 shuttle, means all of the reagents can be added in one pass. The larger volume buffer can be used to wash out the smaller volume of the compound.

  • Page 22: Quadra 4 General Information

    30 inches of bench top space. Tomtec has elected to supply the Quadra 4 in a variety of configurations. This is to allow the end user the freedom of selecting the most cost effective model for their specific requirements.

  • Page 23: Quadra 4 Product Line

    Quadra 4 Product Line QUADRA 4 NS This is the Quadra 4 without the stacker option but with the indexing stage for reformatting i.e., 96 to 384 to 1536. It does not have the full pneumatic system of the Quadra 4.

  • Page 24: Section Iii - Quadra Command System Op Manual

    Section III – Quadra Command System Op Manual Quadra Command System v4 Current Version Level 4.8.x Copyright © 2015. All rights reserved. TOMTEC, Inc. 1000 Sherman Ave. Hamden, CT 06514 Phone: (203) 281-6790 Fax: (203) 248-5724 http://www.tomtec.com Tomtec Quadra 4 Operator Manual...

  • Page 25: Introduction

    1.0 Introduction 1.1 What's New The Quadra 4 Command Software contains several enhancements and improvements over previous versions of the software. Version 4.8.0  The Quadra Command Software now runs on a Windows 7 platform. ®  The Drag and Drop operations have been changed to simple point and Click operations.

  • Page 26: Major Changes To The Quadra 4 Command Software

    1.2 Major Changes to the Quadra 4 Command Software 1.2.1 Drag and Drop Operations When adding labware to a Shuttle Position it is no longer necessary to Drag the labware item and Drop it onto a Shuttle position. Instead simply click the desired Shuttle Position and then click the Labware Item(s) to be placed there.

  • Page 27: Shuttle Labware Labels

    These names can be assigned when the Labware is added to the Shuttle Position, or can be added or changed by Right Clicking on the Shuttle Position icon and selecting the "View/Edit Labware" option. This option can be enabled or disabled through the System Parameters → View Preferences Dialog. Tomtec Quadra 4 Operator Manual...

  • Page 28: Labware And Program Function Icon Changes

    When using the "Not Shown" option, it is important to note that the Labware Icon area will be completely EMPTY when the program is started. The appropriate icons will only be displayed with a Shuttle Position is highlighted. Tomtec Quadra 4 Operator Manual...

  • Page 29: Tip Load Counter

    Shuttle Position 2 is highlighted (as shown above) which already contains a Stacker Nest. Therefore, the only valid Labware choices are Microplates and Reservoirs thus these are the only two icons shown. 1.2.5 Tip Load Counter Tomtec Quadra 4 Operator Manual...

  • Page 30: Psid2 Mirror Rack Checking

    2. The Black Mirror Rack, the White Mirror Rack, and the Destination Plate barcodes all match the values specified within the PSID2 software. In order to use this validation, a barcode reader must be installed on the Quadra 4 on the RIGHT side of the pump.
  • Page 31 Running a protocol with Mirror Rack Checking enabled is done the same way as running any protocol on the Quadra 4. However, during the run, the Quadra software will scan the barcode of the incoming rack and look to find the corresponding PSID2 file in the above directory.

  • Page 32: Quadra 4 - Getting Started

    A distinct advantage of the Quadra 4 is the ability to make a non-contact dispense. The target method may be used to make a clean dispense at the top of the well, without touching the contents of the receiving plate.

  • Page 33: Preparing The Quadra To Run A Program

    This information may be recorded in an initial pause statement. Tomtec personnel are available to assist you in creating a stacker program. When using this program for the first time check that: 1. The Calibration Height is set on all fixtures in use. These values can be set in the Database Editor.

  • Page 34: Main Screen

    2.0 Main Screen The Quadra Main Screen, shown above, contains Six major sections. 1. Labware - Components Area 2. Shuttle Layout Area 3. Pump Volume Box 4. Program Operations Box 5. Program Steps Area 6. Menu Bar Tomtec Quadra 4 Operator Manual...

  • Page 35: Labware - Components Area

    “Options” → “User Preferences” Dialog under the “View Preferences” Tab. Setting the icons to have the Text Descriptions may be useful for users who are unfamiliar with all of the icons. Tomtec Quadra 4 Operator Manual...

  • Page 36: Shuttle Layout Area

    Labware - indicates the current fixture on the shuttle position. Component - indicates the current plate or reservoir on the fixture. Tomtec Quadra 4 Operator Manual...

  • Page 37: Pump Volume Box

    Blue and light blue sections represent liquid volumes, aspirated separately. The example above shows 50uL air gap with 150uL of liquid. If "ERROR" is displayed, then either an over and under volume condition has occurred. Tomtec Quadra 4 Operator Manual...

  • Page 38: Program Operations Box

    “Options” → “User Preferences” Dialog under the “View Preferences” Tab. Setting the icons to have text descriptions may be useful for users who are unfamiliar with all of the icons. Tomtec Quadra 4 Operator Manual...

  • Page 39: Program Steps Area

    Move the selected line(s) UP in the Program Move the selected line(s) DOWN in the Program Program steps can be edited by double clicking on them, or by selecting the Edit → “Edit Step” menu function. Tomtec Quadra 4 Operator Manual...

  • Page 40: Menu Bar

    2.6 Menu Bar The Quadra 4 Menu Bar (shown above) allows you to access many of the Quadra 4 functions. Each menu is described below. 2.6.1 File Menu New - Clears the program window for new program entry. Open - Opens an existing program.

  • Page 41: Edit Menu

    Undelete - Undeletes the last line(s) deleted. Move Up, Move Down - Moves the selected line(s) either up or down in the program. Edit Step - The selected step's function dialog window will be displayed for parameter changes. Tomtec Quadra 4 Operator Manual...

  • Page 42: System Menu

    Configure Model – Refer to the Section “5.4 Configure Model”on page 103. Configure Com Ports – Refer to the Section “5.5 Configure Com Port” On Page 104 Set System Parameters - Refer to the Section “9.0 System Parameters” on page 132. Tomtec Quadra 4 Operator Manual...

  • Page 43: Database Menu

    File Management – Plate & Fixture Databases - Refer to section “10.1 File Management – Plate & Fixture Databases” On Page 143 2.6.5 Tools Menu Serial Dilution Wizard – Refer to Section “7.1 Tools Serial Dilution Wizard” on page 125. Tomtec Quadra 4 Operator Manual...

  • Page 44: Utilities Menu

    Pump Calibration - Refer to Section “6.10 Pump Calibration Tables” on page 121. File Manager – Pump Curve DB – Refer to Section “10.3 File Management – Curve Database” on Page 146. 2.6.7 Options Menu User Preferences – Refer to Section “5.1 User Preferences” on page 99. Tomtec Quadra 4 Operator Manual...

  • Page 45: View Menu

    Show Program Operations - When checked the Functions and Program will be visible. The default is to show (checked). 2.6.9 Reports Menu The "Reports" menu allows the user to view/print Programs, Logs, Databases, and System Parameters. Tomtec Quadra 4 Operator Manual...

  • Page 46: Help Menu

    The online help is the standard windows help engine. Note: The online Help can be brought up at anytime by hitting the F1 key. Quick Start - This invokes the Quadra 4's Quick Start Guide. Getting Started - Quick Start Guide...
  • Page 47 Quadra96 to Quadra 4 Program Conversion - This will guide you through the procedure to convert a Quadra96 program to run on the Quadra 4.Quadra 96 to Quadra 4 Program Conversion Tomtec FileSystem Watcher Service (21CFR Part 11 option) - This section will appear if the optional software package has been installed and is running.

  • Page 48: Program Operations

    Screen. This timer will count down, to show how much time is left before the Alert. When the warning time is reached, the shuttle will turn RED, and the cancel alert button will be displayed. Tomtec Quadra 4 Operator Manual...

  • Page 49: Arm Plate Transfer Operation

    The arm plate transfer operation is used to get plates from and put plates to a stacker nest, vacuum box, or auxiliary station. Conditions Requires a plate/reservoir nest Only shuttle positions 3 & 4 are valid for the Quadra 4 Remarks Set Transfer Type Indicates whether the hand is getting or putting the plate.

  • Page 50: Aspirate Sample

    1 and 4, 4 being the fastest. By default, 3 is automatically selected. Aspirate Height This indicates whether the Aspirate height should be set to its default location near the Bottom of the well, or if another value has been set. Tomtec Quadra 4 Operator Manual...
  • Page 51 The Calibration Table drop down menu consists of user defined pump data for the liquid type used. See documentation on distribution CD. Additionally,refer to Section”3.37 Program Operations Common Options” on page 89 for information on other parameters used by this function. Tomtec Quadra 4 Operator Manual...

  • Page 52: Autotrap Operations

    Important Note: Powered accessories such as Vacuum Traps, Autotraps, and Shaker Nests, should not be connected with the Quadra 4 powered on. Please turn off AC power BEFORE connecting these accessories. Tomtec...

  • Page 53: Delay Program

    Designate the number of minutes the program is suppose to wait, in conjunction with the number of seconds specified, before program continues. Seconds Designate the number of seconds the program is suppose to wait, in conjunction with the number of minutes specified, before the program continues. Tomtec Quadra 4 Operator Manual...

  • Page 54: Dispense Sample

    Aspirate Step will have to be present before a Dispense Step. Remarks Basic Settings Dispense Volume The amount of liquid (in µL) that is to be pipetted into the target plate or reservoir. Tomtec Quadra 4 Operator Manual...
  • Page 55 Dip Tips is another method of capturing hanging drops. Dip Tips is a vertical Touch Off. What this function accomplishes is capturing drops held on the tips by placing the tips in very close proximity to the liquid that has already been dispensed into the wells. Tomtec Quadra 4 Operator Manual...

  • Page 56: Empty Sample

    3.7 Empty Sample To dispense all liquid from tips completely. Conditions To Empty Sample, you will need to select a shuttle position that contains a plate or reservoir capable fixture. Remarks Tomtec Quadra 4 Operator Manual...
  • Page 57 Dispense function has completed. Additionally, refer to Section “3.37 Program Operations Common Options” on page 89 for information on other parameters used by this function. Tomtec Quadra 4 Operator Manual...

  • Page 58: External Device

    There are no requirements to run this step. Remarks External Device Channel The Quadra 4 has two dedicated external control channels. Quadra 4 User Defined Outputs Contact OUT Action The user can select to do nothing, No Action, toggle the contact, Contact Closed, Contact Open, or toggle the contact with a timed pause, Contact Closed, Pause, Contact Open.

  • Page 59: Gosub Program

    Main Program continues. A Text box will appear after the Pause function is selected, giving the user an opportunity to generate a message to be displayed when the program pauses Tomtec Quadra 4 Operator Manual...

  • Page 60: Goto Program

    A Text box will appear after the Pause function is selected, giving the user an opportunity to generate a message to be displayed when the program pauses. Tomtec Quadra 4 Operator Manual...

  • Page 61: Load Tips

    That is if a 50ul tip fixture is selected, all liquid handling functions after the tip load will set their maximum volume to 50ul. The firmware will set its maximum volume to 50ul. This is done to prevent any pump range errors. Tomtec Quadra 4 Operator Manual...

  • Page 62: Lockout Shuttle Position

    Designates the number of seconds to prevent operation on that position. 3.13 Loop Enables the user to repeat a series of steps and allows the user the ability to perform repetitive stacker functions. Conditions There are no requirements for this function. Tomtec Quadra 4 Operator Manual...

  • Page 63: Magnetic Nest

    The magnetic surface or probes are moved by programming the Quadra to deliver pressurized air to the nest bladder which raises the magnetic platform Conditions A Magnetic Nest has to be present on the selected shuttle position. Tomtec Quadra 4 Operator Manual...

  • Page 64: Mix Sample

    Alternating aspirate and dispense steps to achieve a uniform suspension or thorough mixing of chemicals, reagents, and/or buffers. Conditions To Mix, you will need to select a shuttle position that contains a plate or reservoir capable fixture. Tomtec Quadra 4 Operator Manual...
  • Page 65 The Mix Volume is the amount of liquid (in µL) that is to be aspirated and dispensed to the designated well for x number of cycles. Air Gap/Blow Out See Aspirate / Dispense Function. Blow Out Height See Dispense Function. Touch Off See Dispense Function. Dip Tips See Dispense Function. Tomtec Quadra 4 Operator Manual...

  • Page 66: Move Arm

    The move arm operation is used to change the position of the device as well as open / close the hand. Conditions None Remarks Set Hand (before move) Indicates the state of the hand before and during the move. Tomtec Quadra 4 Operator Manual...

  • Page 67: Move Shuttle

    Allows a simple move of the shuttle to any position. Conditions There are no physical requirements for this function. Remarks Shuttle Position Select the shuttle position that will be moved. The position selected is positioned above the stage. Tomtec Quadra 4 Operator Manual...

  • Page 68: Move Stage

    3.18 Move Stage Moves the stage to the specified height and position. Conditions There are no requirements for this operation. Remarks Tomtec Quadra 4 Operator Manual...

  • Page 69: Nitrogen Dry

    A 450µL backfill pump with the optional nitrogen gas valve installed is required. Remarks Seconds indicates the amount of time for nitrogen gas to be blown out the tips. Height indicates the desired stage position to perform the operation. Tomtec Quadra 4 Operator Manual...

  • Page 70: Pause

    By selecting a shuttle position, the program pauses at the specified shuttle position and waits for user intervention. Message In the Text Box below, the user has an opportunity to generate a message that will appear on the screen when the system pauses. Tomtec Quadra 4 Operator Manual...

  • Page 71: Pressure Control

    Remarks Set Pressure Sets the pressure regulator from 0 (off) to 15 PSI. 3.22 Program Note This is a non-function. "Note" is a comment line added to the program for any reason. Conditions None Remarks Tomtec Quadra 4 Operator Manual...

  • Page 72: Quit

    Restack Plates is the repeated Infeed of a plate from one stacker and Outfeed that plate to another stacker. Conditions A plate capable fixture must be located on a shuttle position that has access to both the source and destination stackers. Remarks Tomtec Quadra 4 Operator Manual...

  • Page 73: Serial Dilution

    Conditions The Serial Dilution function requires a selected shuttle position. Note: This function is only available when a removable tip pump is installed. Tomtec Quadra 4 Operator Manual...
  • Page 74 Initial Dispense Volume The initial concentration pipetted from a sample plate. Plate Direction The direction in which the dilution is performed. This depends on the plate and tip arrangement. Tomtec Quadra 4 Operator Manual...

  • Page 75: Shaker Nest

    Quadra. The oscillation amplitude is controlled by a dial on the Shaker Nest. Conditions You must have a shaker nest on the specified shuttle position, properly connected to the Quadra in order for this function to work. Tomtec Quadra 4 Operator Manual...
  • Page 76 Remarks Important Note: Powered accessories such as Vacuum Traps, Autotraps and Shaker Nests, should not be connected with the Quadra 4 powered on. Please turn off AC power BEFORE connecting these accessories. Specify which shaker nest to control, and whether or not to switch the device on or off.

  • Page 77: Shuck Tips

    SPE Block currently loaded. In this case the “Shuck Tips” verbiage will be replaced by “UnLoad SPE”. 3.28 Shuttle Layout Change Allows the user to pause the program that is running, and change the fixtures and components loaded onto the shuttle. Tomtec Quadra 4 Operator Manual...

  • Page 78: Spe Pressure Vacuum Operation

    The SPE Pressure/Vacuum function allows for both positive pressure and vacuum to be applied to a filter plate. Conditions This function requires that the vacuum controlled mini-trap and the software controlled pressure regulator options be installed. Tomtec Quadra 4 Operator Manual...
  • Page 79 When checked, a puff of air will be cycled to free the plate from the pump pressure fixture. Vacuum Pressure Relief Time Time required to bleed off the vacuum/pressure to free the plate from the vacuum box. Tomtec Quadra 4 Operator Manual...

  • Page 80: Spe Vacuum Box Operation

    The SPE Vacuum Box operation requires a vacuum box and a vacuum controlled Mini- Trap. Remarks Important Note: Powered accessories such as Vacuum Traps, Autotraps and Shaker Nests, should not be connected with the Quadra 4 powered on. Please turn off AC power BEFORE connecting these accessories. Tomtec Quadra 4 Operator Manual...

  • Page 81: Stacker Operations

    Conditions The Stacker Operations Function requires you to select a shuttle position that has a plate capable fixture. The fixture also has to allow stacker related functions. Remarks Tomtec Quadra 4 Operator Manual...

  • Page 82: Target Method

    This is necessary for thin plates. 3.32 Target Method The "Target Method" function is available for the 450ul Timed Dispense head and the 20/50/250ul DMV head. The function is a tightly coupled Aspirate and Dispense of small volumes. Remarks Tomtec Quadra 4 Operator Manual...
  • Page 83 The small 3µL volume and 3 psi driving force, accelerates the liquid fast enough to minimize or eliminate the clinging drops on the tip exterior. Tomtec Quadra 4 Operator Manual...

  • Page 84: Timed Dispense

    The number of times the pinch tube clamp opens to deliver air. Height The height which the tips will deliver air to the well. Note: Check air pressure to avoid high pressure dispenses that spill liquid (this can be done from the Utility). Tomtec Quadra 4 Operator Manual...

  • Page 85: Vacuum Box Operation

    If Run Raw is checked then the vacuum will just turn off. Raise Tray performs vacuum off and then raises the tray. If Run Raw is checked then the tray will just raise (Warning: It is not recommended to raise the tray while vacuum is on). Tomtec Quadra 4 Operator Manual...

  • Page 86: Vacuum Control

    Important Note: Powered accessories such as Vacuum Traps, Autotraps and Shaker Nests, should not be connected with the Quadra 4 powered on. Please turn off AC power BEFORE connecting these accessories. Tomtec...

  • Page 87: Wash

    This function requires Ultrasonic Wash to be on the specified shuttle position in order for this function to operate properly. Remarks Blow Out Volume See Dispense Function. Blow Out Height See Dispense Function. Mix/Wash Volume See Mix Function. Tomtec Quadra 4 Operator Manual...
  • Page 88 Dry/Rinse Tips The Dry/Rinse Tips option allows the user to blow out an air gap at the end of the wash. Timed Dispense See Timed Dispense Function. Drop Stage after Tip Wash See Additional Options. Tomtec Quadra 4 Operator Manual...

  • Page 89: Program Operations Common Options

    This rate of adjustment is based on the Well Rate parameters assigned in the micro-plate and reservoir database. Tomtec Quadra 4 Operator Manual...

  • Page 90: Dip Tips

    1000, the stage will move 19 steps to 1019 and then back to 1000. 3.37.3 Select Shuttle Position This function allows the user to change the shuttle position of a step without having to recreate the step. Grayed positions are unavailable for the current function. Tomtec Quadra 4 Operator Manual...

  • Page 91: Stage Operations

    96 tip head, and pipette to a 384-Well plate. Quad & Row/Column Offsets Note, since the Quadra 4 SPE does not have an indexing stage, the Stage Operations selection is locked out. Using the Quadrant Formatter feature, it is easy to take a standard 96 Tip head, and format it to pipette from a 384-Well Plate.
  • Page 92 Cartesian plane (although the stage actually moves.) In a standard 96-Well plate, there are 577 steps between each well, and in a standard 384- Well plate, there are 288 steps between each well. Here are some more examples: Tomtec Quadra 4 Operator Manual...

  • Page 93: Touch Off

    The XY, and XY -X -Y touch offs are 2 and 4 point movements. All touch off movements start from the center of the well to the offset and back to the center for each point. Tomtec Quadra 4 Operator Manual...

  • Page 94: Database Editor

    Tomtec’s Quadra design allows another important pipetting feature. It provides the ability to blow low air pressure through the tip orifices. This is referred to as the “Timed Dispense Function”.

  • Page 95: The Menu Bar

    Tomtec’s software provides a third tip height in relation to the well. This is “Clear Height”. This is defined when the tips are .060 inches above the top of the wells. This height is used if the stage must index the plate under the tips, for serial dilution as an example.

  • Page 96: The Plate Panel

    Bottom Steps is the number of steps the stage has to travel beyond the fixture in order for the tips to touch the bottom of the wells. (Not necessarily the bottom of the plate or reservoir.) Tomtec Quadra 4 Operator Manual...

  • Page 97: The Fixture Panel

    After setting the Load Height, if you are unsatisfied with the result, you may click the Reset Calibration button to return to the original value, only before you save. The application will prompt you to save any changes when you switch records, or close the application. Tomtec Quadra 4 Operator Manual...

  • Page 98: The Touch Off Panel

    Click on the Add and Remove buttons to carry records from one side to another. When you are finished selecting those items you wish to import, click on the Import button to finalize the procedure. Tomtec Quadra 4 Operator Manual...

  • Page 99: Options

    Auto - Disconnect On Exit When disabled, the operator has to use the "Disconnect" tool bar or menu item to shut down the Quadra. When enabled, the application will automatically call the "Disconnect" function when the application is exited. Tomtec Quadra 4 Operator Manual...
  • Page 100 When enabled it will open the pinch tubes after a ten second count down: The operator has the option to abort the operation anytime during the countdown. When this option is disabled, the operator will be prompted to open the pinch tubes. Tomtec Quadra 4 Operator Manual...

  • Page 101: Default Plates

    When this button is pressed, all non-standard plates that are in the database will be displayed. All Plates When this button is pressed, all plates that are in the database will be displayed. When this button is pressed, the selected plate will be moved to the default list. Tomtec Quadra 4 Operator Manual...

  • Page 102: Default Reservoirs

    5.3 Default Reservoirs When this button is pressed, the selected reservoir will be moved to the default list. Delete When this button is pressed, the selected reservoir in the default list (lower dialog box) will be removed. Tomtec Quadra 4 Operator Manual...

  • Page 103: Configure Model

    Functions Palette The operator can select or deselect which optional functions to display. Note that only the functions buttons are affected. Editing of functions not displayed on the buttons, but in the program is allowed. Tomtec Quadra 4 Operator Manual...

  • Page 104: Configure Com Port

    5.5 Configure Com Port Com Port The operator can set the serial com port (1 to 32), the baud rate, and flow control. These values are set at the factory and should not need to be changed. Tomtec Quadra 4 Operator Manual...

  • Page 105: Set User Defined Output Names

    Note if the Quadra AC Control Interface Module is used, right output 1 will control the vacuum pump, and right output 2 will control the ultra sonic tip wash pump. Outputs three(3) and six(6) are used by the External Device program operation. Tomtec Quadra 4 Operator Manual...

  • Page 106: Set Arm Position Names And Heights

    Open the hand and position it as desired. Upon exiting the Utility dialog a prompt will occur to automatically set the value to the current arm position. Upon exiting the user defined arm heights dialog a prompt will occur to retract the arm if it is extended. 5.8 Displayed Fixtures Tomtec Quadra 4 Operator Manual...

  • Page 107: Edit Display And Order Of Fixtures

    This function will remove the selected fixture from the display list. This function will move the selected fixture up in the display list order. Down This function will move the selected fixture down in the display list order. Tomtec Quadra 4 Operator Manual...

  • Page 108: Current File Settings Options

    "NO READ Errors" occur when the reader is unable to properly scan the bar code on the applicable plate. Some conditions that would generate this error include a missing bar code label or bar code label is on the wrong side. Tomtec Quadra 4 Operator Manual...

  • Page 109: Runtime Database Usage Options

    The default is to use the plate and fixture database heights, which requires plates and fixtures present at runtime. You might want to check this if you are moving a program to another system where plates and fixtures may not be in the database. Tomtec Quadra 4 Operator Manual...

  • Page 110: View Preferences

    5.10 View Preferences These options are described in Section “1.2.4 Labware and Program Function Icon Changes”, on page 28. Tomtec Quadra 4 Operator Manual...

  • Page 111: Utility

    This is necessary for thin plates that do not have lids. will reset all stacker elevators to the down position. This Initialize Stackers would be useful in the event of a stacker lost or interlock error has occurred. Tomtec Quadra 4 Operator Manual...

  • Page 112: Stage

    Note: If a stage safety occurred, the stage override UP/DOWN can be enabled by holding down the "Alt" key when clicking on the stage up/down arrows. The Stage MUST be reinitialized after clearing the safety error. Tomtec Quadra 4 Operator Manual...
  • Page 113 Select the button to move to the desired Quadrant. WARNING: Stage indexing will occur at its current height. Make sure that the plate has sufficient clearance of the tips and the stage is not completely below the shuttle. Tomtec Quadra 4 Operator Manual...

  • Page 114: Pinch Tubes

    Set Pinch Tubes Open Pinch Tubes will open the inch tubes. Close Pinch Tubes will close the pinch tubes. Pressure Valve The pressure valve can be closed to keep the compressor from constantly running. Tomtec Quadra 4 Operator Manual...

  • Page 115: Pump

    "DMV" head is detected, the tip volume can be set. The tip volume limits the aspirate and dispense function's maximum volume. Shuck Tips will shuck tips from the pump to the current shuttle position. Make sure the appropriate tip jig is present. Tomtec Quadra 4 Operator Manual...

  • Page 116: System

    Initialize System will reset all devices on the Quadra to their default positions. 6.6 Trap Operations Standard Trap: Trap Control Refresh Status will update the adjacent progress bar to indicate the current level of fluid in the flow wash trap. Empty Trap will empty the trap Tomtec Quadra 4 Operator Manual...
  • Page 117 Vac On turn on the vacuum to the vacuum box. Important Note: Powered accessories such as Vacuum Traps, Autotraps and Shaker Nests, should not be connected with the Quadra 4 powered on. Please turn off AC power BEFORE connecting these accessories. Tomtec...

  • Page 118: Miscellaneous

    Note: The air pressure is changed by turning the black gauge knob on the side of the Quadra. Important Note: Powered accessories such as Vacuum Trap and Shaker Nests, should not be connected with the Quadra 4 powered on. Please turn off AC power BEFORE connecting these accessories. Tomtec Quadra 4 Operator Manual...

  • Page 119: Arm - Vacuum Box

    Initialize Arm will reset the arm. Set Vacuum Box Vacuum On/Off turns the vacuum on or off. Open/Close Box opens or closes the box top Tray Up/Down raises or lowers the tray. Make sure the vacuum is off first. Tomtec Quadra 4 Operator Manual...

  • Page 120: Status

    6.9 Status The light switch status utility gives the user a real time assessment of the Quadra 4's inputs. When depressed, the status of the pump, stage, shuttle, stackers, trap, arm, interlocks, and auxiliary inputs are displayed. Tomtec Quadra 4 Operator Manual...

  • Page 121: Pump Calibration Tables

    6.10 Pump Calibration Tables The pump calibration tables are supplied by Tomtec to insure the accuracy of the pump over its range. The curve(s) supplied are in a special ".csv" format. The data entry application reads in the supplied data, displays the curve, and saves it in the "QCURVEDB.DAT"...
  • Page 122 The "csv" formatted curve supplied by Tomtec can be selected, read in and displayed: The file is checked against any duplicate named curves. If a duplicate is found, the operation will be prompted: A "Yes" reply will add " - Ver: xx" after the curve name.
  • Page 123 + requestedlow steps = (calvol * stepsperul) /1000.0 The above data is "Micro Logged" as: CalI: Vol: 135.0, Data: ReqL: 125.0 MeasL: 125.8600 ReqH: 200.0 MeasH: 207.5100, CalVol: 133.39559094, Steps: 2134 = 133395.59094 * 16) / 1000.0 Tomtec Quadra 4 Operator Manual...

  • Page 124: Utility User Outputs

    The On/Off buttons control and show the status of outputs 1 to 6. Note that outputs three(3) AND six(6) are used by the External Device program operation. Refresh Refreshes the status of the On/Off buttons. Tomtec Quadra 4 Operator Manual...

  • Page 125: Tools

    Conditions The Serial Dilution Wizard requires a selected shuttle position. 7.1.1 Setup Sample Source Position Select the source plate shuttle position. Dilution Destination Position Select the destination plate shuttle position. Sample and Dilution Volumes Tomtec Quadra 4 Operator Manual...

  • Page 126: Set Source Row/Column

    If the source plate is a 96 well plate, and a preset tip arrangement is selected, the row or column for the initial Sample Volume aspirate can be selected by clicking on the plate graphic. Back The "< Back" button moves the wizard back to the previous page Tomtec Quadra 4 Operator Manual...

  • Page 127: Set Sample Aspirate Volume And Height, Row, And Column Offsets

    7.1.3 Set Sample Aspirate Volume and Height, Row, and Column Offsets See Aspirate Function for more details. Tomtec Quadra 4 Operator Manual...

  • Page 128: Set Initial Dilution Row/Column

    If the destination plate is a 96 well plate, and a preset tip arrangement is selected, the row or column for the initial Dispense Volume can be selected by clicking on the plate graphic. 7.1.5 Serial Dilution See Serial Dilution Function for more details. Tomtec Quadra 4 Operator Manual...

  • Page 129: Technical Tips

    3 can access the front & rear stackers so they are too valuable. Of the 40 micro-titer plates, only two are being used at the same time and therefore will only occupy 2 shuttle positions. Tomtec Quadra 4 Operator Manual...

  • Page 130: Writing The Protocol

    For more efficient mixing, aspirate off the bottom of the well and dispense higher up in the well. The magnetic nest needs to be turned on in position. Tomtec Quadra 4 Operator Manual...
  • Page 131 The step has now gone from being step two to step three. Keep pressing the move down icon until the end loop is the last step. The protocol is now completed and ready to run. Tomtec Quadra 4 Operator Manual...

  • Page 132: System Parameters

    9.0 System Parameters The Quadra 4 firmware parameters are set by qualified Tomtec personnel during assembly. Conditions A password is required to change all but a few non-critical system parameters. If a critical parameter has to be changed, you should contact Tomtec's Technical Support department at 203.281.6790.

  • Page 133: System Parameters - Stage

    9.1 System Parameters – Stage Tomtec Quadra 4 Operator Manual...

  • Page 134: System Parameters - Shuttle

    9.2 System Parameters – Shuttle Tomtec Quadra 4 Operator Manual...

  • Page 135: System Parameters - Pump

    9.3 System Parameters – Pump Tomtec Quadra 4 Operator Manual...

  • Page 136: System Parameters - Miscellaneous

    9.4 System Parameters – Miscellaneous Tomtec Quadra 4 Operator Manual...

  • Page 137: System Parameters - System Info

    9.5 System Parameters - System Info System Firmware and Software Version levels, Board Check Sums, and Serial Number data. Serial Number - The serial number is entered by Tomtec personnel when setting up the instrument. Tomtec Quadra 4 Operator Manual...

  • Page 138: System Parameters - Shuttle Alignment Utility

    1 to 6 continuously until aborted by the stop button. Note: that once the end positions, Front, Rear, Left and Right, are set. They should be the same for all shuttle positions Tomtec Quadra 4 Operator Manual...

  • Page 139: System Parameters - View System Parameters

    This utility allows the user to view, print or save to a text file the system settings. Note: the default parameter file (Q4SYSPARM.DAT) is displayed. User named files can be displayed by using the "Open File" button. Tomtec Quadra 4 Operator Manual...

  • Page 140: Vacuum Calibration

    Passes The number of 0 to 25 inch passes to perform. The default is 3. Apply accepts the calibration values for inclusion into the configuration file. records & saves the values for program use. Tomtec Quadra 4 Operator Manual...

  • Page 141: File Management

    This file can be shared between any model Quadra 4, and it can also be saved to either a local folder or on a server. The default location for this file is in the application's database folder.
  • Page 142 The programs can be write protected by using the windows file management, EXPLORE.EXE. program_name.log - Quadra 4's program runtime log. The log file is created when the program is run. it has the same name as the program, or it can have the a time-date stamp incorporated in the file name.

  • Page 143: File Management - Plate & Fixture Databases

    Q4FIXTUREDB.DAT, contains nest, tip jig, tips, wash station, and other Labware. The plate and fixture databases can be shared by multiple Quadra 4's. To do this the Quadra 4's have to be networked with a central location for the databases. Note that this location can be write protected using windows file management (Sharing &...
  • Page 144 The databases can be backed up to a remote folder. This folder can be either on the Quadra 4 or if networked, on a server. Tomtec Quadra 4 Operator Manual...

  • Page 145: File Management - Configuration Database

    10.2 File Management – Configuration Database The Quadra 4 configuration database, Q4SOFTCFG.DAT, can be shared by other Quadras. This is handy feature if you have multiple Quadra 4's with the SAME hardware configuration. The file can also be backed up to a server.

  • Page 146: File Management - Curve Database

    Note that when backing up to a server, a separate folder must be created for each Quadra 4. If this is not done, the saved curve database will be useless. If multiple Quadra 4's run the same set of programs, the Curve Name may be different between the Quadras.
  • Page 147 The highlighted fields, CURVE NAME="", DATESTRING="", and DATE="" have to by synced on the Quadra. The data between the "s should be the same for all units. Tomtec Quadra 4 Operator Manual...

  • Page 148: File Management - System Parameters Database

    10.4 File Management – System Parameters Database The Quadra 4's system parameters, Q4SYSPARM.DAT, file is UNIQUE and CANNOT be shared. It can be backed up to either another folder or a server. Note that this file is used to verify the machine's setup parameters on the initial connect. If any parameters have changed, they will be corrected to the setting in the file.

  • Page 149: Tomtec Filesystem Watcher Service

    11.1 FileSystem Watch Marketing Information Overview The Tomtec File System Monitor and Logging service is one component in a CFR11 compliant system. The other component is the Operating System that runs the software. The Operating system is responsible for general security.

  • Page 150: File System Monitoring

    CFR11 documentation on the FDA website, and look at the real information that is there it is actually quite simple. • Who did it. • When did they do it. • What did they do. • Be able to reproduce it. Tomtec Quadra 4 Operator Manual...

  • Page 151: Installation

    (a) The regulations in this part set forth the criteria under which the agency considers electronic records, electronic signatures, and handwritten signatures executed to electronic records to be trustworthy, reliable, and generally equivalent to paper records and handwritten signatures executed on paper. Tomtec Quadra 4 Operator Manual...
  • Page 152 (e.g., specific center, office, division, branch) to which such submissions may be made. Documents to agency receiving unit(s) not specified in the public docket will not be considered as official if they are submitted in electronic form; Tomtec Quadra 4 Operator Manual...
  • Page 153 (9) Open system means an environment in which system access is not controlled by persons who are responsible for the content of electronic records that are on the system. Tomtec Quadra 4 Operator Manual...

  • Page 154: Filesystem Installation Information

    Source: 62 FR 13464, Mar. 20, 1997, unless otherwise noted. 11.2 FileSystem Installation Information 11.2.1 Overview The Tomtec File System Monitor and Logging Service (FSMLS) is a component of Tomtec software’s CFR11 compliance system. This program’s purpose is to store all user programs, and to store all machine operational parameters.

  • Page 155: Configuration

    Installation of .Net framework 2.0 The .net framework 2.0 is a Microsoft product, Tomtec has included the Microsoft installer on the installation CD, but the user is free to download directly from Microsoft, either through windows update or any other means. The installer is pretty straightforward, and will indicate to you any other items that must be installed prior to the framework.

  • Page 156: Usage

    11.2.4 Usage a. Usage of logging The logging portion of the FSMLS is transparent to the user, if the user is using one of the supported GUI applications. The currently supported platforms are: Tomtec Quadra 4 Operator Manual...

  • Page 157: Os Requirements

    Dotnetfx.exe “.Net framework 2.0” WindowsInstaller-KB893803-v2-x86.exe “Windows Installer 3.1” Windows XP-KB835935-SP2-ENU.exe “Windows XP service pack 2” These are all English language versions. For other languages, all of these products can be downloaded from Windows update http://windowsupdate.microsoft.com/ . Tomtec Quadra 4 Operator Manual...

  • Page 158: Quick Start

    • Install .net framework 2.0 if it isn't already. • Visit windows update, not required but recommended. • Run setup.exe from the install CD. • Launch the configuration program. • Configure to needs. • Start service. Tomtec Quadra 4 Operator Manual...

  • Page 159: Providing 21Cfr Part 11 Security For The Quadra 4

    ( presumably the FSWatch directories will receive similar protection ). Running a Quadra 4 program will leave a log file in the designated directory. Because we configured FSWatch to monitor that directory, writing the file will be noted in the...
  • Page 160 C:\LogDir\C\Program Files\Tomtec\Quadra 4 Command System v4\logs contains a copy of each file which existed during that time: Now suppose that someone attempts to use an editor to modify that log file. Because FSWatch continues to monitor the contents of the specified directory, any changes to the file will be logged at the end of the FSWatch log: and copies of the file ( in this case saved as Sup Transfer_50ul.0.qpg, etc.
  • Page 161 In addition to using the FSWatch system to monitor the security of files, a Quadra system also provides several reports which of themselves contribute to meeting CFR 11 requirements (and, of course, these are some of the files whose integrity should be monitored using the FSWatch system). Tomtec Quadra 4 Operator Manual...
  • Page 162 End Of Run Report Runtime Log: Tomtec Quadra 4 Operator Manual...

  • Page 163: Section Iv - Pipettor Heads

    1.0 Quadra 4 Air Displacement Pipettor Heads 1.1 Quadra 450µL Disposable Tips This is the primary pipettor head for the Quadra 4. It is the dominant pipettor for general applications with a long history of reliability and dependable operation. It has several primary advantages.

  • Page 164: Non Contact Dispensing

    96 individual pipettor pistons. Tips may be removed for 48 well pipetting or 24 well pipetting. With the indexing stage of the Quadra 4, a single row of tips may be used in the 8 or 12 direction for serial dilution applications.

  • Page 165: Adjusting Tip Load Switch

    If there is a problem loading tips, it is typically due to one of two causes: 1. The primary cause of the tip load failure can be loss of lubrication on the tip pin “O” rings. Use the tip pin lubricator that was supplied with the Quadra (TOMTEC P/N 196- 107).

  • Page 166: Quadra 4 Positive Displacement Pipettor Heads

    In all of the testing by Tomtec and others, we have not been able to measure any carry- over with Fluorescence, Colorimetric, and Scintillation detection. In all cases, the carry over wells were equivalent to the blank wells.

  • Page 167: Quadra 4 Disposable Multi Volume Head (Dmv)

    4.0 Quadra 4 Disposable Multi Volume Head (DMV) QUADRA 4 – DMV PIPETTING HEAD The Quadra 4 has an optional DMV Pipetting Head that offers the user the ability to operate the instrument with three different volume pipetting tips with one pipetting head.
  • Page 168 20) Select OK 21) Return to Tip Utility 22) Select System Utilities 23) Select Shuck Tips 24) Place another set of pipette tips (different volume) on the nest 25) Repeat procedure for other tip volumes Tomtec Quadra 4 Operator Manual...
  • Page 169 ELECTRONIC (Z) OFFSET PROCEDURE 1) The DMV pipette head should be installed and aligned in the Quadra 4 instrument. 2) Turn on Quadra 4 POWER 3) Connect and Initialize the Quadra 4 4) Place actual Tip Load Fixture under pipetting head...

  • Page 170: Setting The Zero Volume Adjustment For Air Displacement Pipettor Heads

    For this procedure, a Zero Volume Setting Adjustment Gauge is required: 450uL AIR TIP SHUCK PIPETTING HEAD 026929 0.156” (3.062mm) DMV AIR TIP SHUCK PIPETTING HEAD 026928 0.110” (2.794mm) (1) Under “System Parameters” select “Pump Zero Volume Alignment Utility”. Tomtec Quadra 4 Operator Manual...
  • Page 171 (5) Select “Record Max Travel” button. Same number of steps, as #4, will be Displayed. (6) Select “Set Parameter” button. (7) Select “Initialize Pump” button to re-initialize pump. (8) Select “OK” button. Pump Zero Setting is now set into factory settings. Tomtec Quadra 4 Operator Manual...

  • Page 172: Quadra Shuttle

    6.0 Quadra Shuttle The concept of the shuttle is what sets the Quadra 4 apart from its competition. The shuttle can take plates from any of the stackers and move them to and from the pipettor. On a flat bed pipettor, a separate device, such as a conveyor or robotic arm, is required. The six stations of the shuttle add to its utility.

  • Page 173: Quadra Stage

    An error signal is generated at that time. 8.0 Quadra Stage The stage lifts the plate nests from the Quadra 4 shuttle to the tips. Four pins on the top of the stage locate into the corresponding holes on the bottom of the nests. This permits the precision necessary for 384 and 1536 operation.
  • Page 174 When "escape" is pressed, a "jog stage "window will appear on the screen. The stage can then be "jogged" (moved) accordingly using "Page Up" or "Page Down" to remove any obstacle. DO NOT MOVE THE STAGE BY HAND-DAMAGE MAY OCCUR. Tomtec Quadra 4 Operator Manual...

  • Page 175: Section V - Suggested Pipetting Methods & Recommendations

    Normally, aspirations are made near the bottom of the container. These values are set in the Quadra 4 Database. When setting these values, do not pin the tip to the bottom of the well. The software provides for "slow withdrawal". The speed with which the tip leaves the liquid has an effect on drops that can cling to the tip exterior.

  • Page 176: Dispensing

    As an example, assume the protocol requires 50µL of buffer, 30 microliters of reagents A, 20 microliter of reagent B, and 10µL of drug compound. The Quadra 4 shuttle will present these devices in order to the pipettor head. First is to aspirate 50µL of buffer, then 10µL of air gap, followed by 30µL of reagent A, then 10µL of air...

  • Page 177: Bubbling

    The air pressure is set by the black knob on the side that regulates the air pressure in the internal air source. 1 to 2psi is recommended as a starting point. Tomtec Quadra 4 Operator Manual...

  • Page 178: Section Vi - Pipetting With The Positive Displacement Pipettor Head

    2.0 Dispensing 0.5µl spots - Aqueous There are some applications that 0.5µL aliquots need to be dispensed into a dry well without the use of washing out. Testing has shown the following when pipetting aqueous solutions. Tomtec Quadra 4 Operator Manual...

  • Page 179: Dispensing 0.5Μl Spots - Dmso

    On a mix function, is liquid level following the piston motion? If not, slow the aspirate/dispense speeds. d. Are the tips being cleared on a dispense? e. Is there liquid clinging to the tip exterior? Tomtec Quadra 4 Operator Manual...

  • Page 180: Section Vii - Bioanalytical Applications

    BioAnalytical applications. It lives on its history of use in the Quadra 96 Model 320 and Quadra 3. Quadra 4 platform is the third generation design and may be supplied with stackers and automated filtration for full walk away automation.

  • Page 181: Protocol Or Program Security

    While the Quadra 4 calculates the checksum each time any program is called, it does not verify that it matches a previous record. This must be accomplished external to the Quadra 4 normal software configuration.
  • Page 182 25µL, the accuracy will be within 3%. From 25µL to10µL, the accu- racy will be within 5%. The user may create a specific calibration curve for the pipettor head in the Quadra 4. This will improve the accuracy. With specific calibration, accuracy of less than 1% can be obtained from 100µL...
  • Page 183 Quadra 4 with the "Zero" of the command control software. Making a new entry in the database is intuitive. Open to the Quadra 4 and software initialize the instrument. Click on the database on the tool bar, follow the description.

  • Page 184: Section Viii - Quadra 4 Accessories

    Section VIII - Quadra 4 Accessories Tomtec provides a wide variety of accessories for the Quadra 4 to expand its value for specific applications. 1.0 Custom Reservoirs Tomtec will specially fabricate custom reservoirs to match your pipetting pattern. For example, many clients leave the first row of wells open without compounds to facilitate adding standards and controls to the pattern.

  • Page 185: Constant Level Reservoir

    This is simply a block of aluminum that provides thermal mass for a reservoir. The aluminum may be heated or cooled before hand and will exchange heat over a limited period of time. This is not stacker accessible. Tomtec Quadra 4 Operator Manual...

  • Page 186: Peltier Nest

    This is a 3 part vacuum box for SPE applications. It provides means of capturing the diluent in a microplate or deepwell plate. It is manually operated. 1.9 Filtration System for the Quadra 4 This system consists of a manually operated vacuum box, and a manually regulated mini vacuum trap.

  • Page 187: Auto Regulated Mini Vacuum Trap

    This must be factory installed. This system consists of a robotic hand for plate transfer. A fully pneumatically operated vacuum box and a vacuum regulated mini trap. It is designed to provide a walk-away automation for all filter plate protocols. Tomtec Quadra 4 Operator Manual...

  • Page 188: Section Ix - Pipetting Recommendations

    Set the tip height carefully to obtain 100% touch off of all needles. If set too loose or too tight to the late well bottom, some spots will be missed. There is about a 0.010 inch (thickness of three sheets of copy paper) variation that is allowed for 100% touch off. Tomtec Quadra 4 Operator Manual...

  • Page 189: Tip #5

    When mixing reagents in the well, aspirate from the bottom of the well and dispense above the final liquid level. This will turn the liquid over. It is recommended that you mix with 70-75% of the well volume. Tomtec Quadra 4 Operator Manual...

  • Page 190: Section X - Calibration Of The Quadra 4 ® 450Μl

    Otherwise, cumulative error will appear in the last volume. The QUADRA 4 calibration software, recognizes this condition, by comparing the asked for aspirated volume to the dispensed volume to prevent an out of range error from occurring.

  • Page 191: Equipment Required

    Weighing Vessels – (60) Used to Create a calibration curve (30) Used to Verify the calibration curve Cat. No. 320-50 96 Well Tip Loading Jig  (Supplied with the QUADRA 4 Cat. No. 196-130-00 Polypropylene Reagent Reservoir with 3 baffles  (Supplied with the QUADRA 4 Cat.

  • Page 192: Calibration Curve Procedure

    110-2 110-3 125µL 125-1 125-2 125-3 150µL 150-1 150-2 150-3 200µL 200-1 200-2 200-3 300µL 300-1 300-2 300-3 450µL 450-1 450-2 450-3 Using the 4 Place Balance – Record tare weight of each Weighing Vessel. Tomtec Quadra 4 Operator Manual...

  • Page 193: Verification Of Calibration Curve Procedure

    To keep the error, due to evaporation, as small as possible the following should be considered: A balance with the appropriate accessories such as an evaporation trap. Pipetting to weighing Cycle Time. Tomtec Quadra 4 Operator Manual...

  • Page 194: Pipetting To Weighing Cycle Time

    Click on the “CONNECT TO SYSTEM” button.  Click on the “INITIALIZE” button to initialize the QUADRA 4 Verify the Air Pressure for BLOW-OUT is set between 1.00 and 1.25psi. Verify that the Backlash Factor, per factory specifications, has been set into the Instrument Parameters of the Quadra.

  • Page 195: Calibration Template - Q4" Spreadsheet

    TIME TEMP º C (To nearest 0.2ºC) HUMIDITY % (To nearest 10%) BAROMETRIC PRESSURE (in. Hg) HEAD SERIAL NUMBER Fill in the tare weight of each Calibration weighing Vessel in the area marked “TARE WT.”. Tomtec Quadra 4 Operator Manual...

  • Page 196: Creating A New Calibration Curve With Water

    4. The average % Error of the 3 weights for each set volume is used in creating the Calibration Curve data points. 5 Tomtec provides a spreadsheet to calculate the % ERROR. It also calculates the mean, standard deviation, and CV of the specific samples that are entered for each volume.
  • Page 197 Continue this routine using the supplied programs for the remaining 15 SET VOLUMES (10L to 450L ) doing 3 pipettings of each volume. Shuck and dispose of the QUADRA Pipetting Tips using the “SHUCK TIPS” program. Tomtec Quadra 4 Operator Manual...

  • Page 198: Creating A Verification Curve

    5. The average % Error of the 3 weights for each set volume is used in creating the Verification Curve data points. 6 Tomtec provides a spreadsheet to calculate the % ERROR. It also calculates the mean, standard deviation, and CV of the specific samples that are entered for each volume.
  • Page 199 Continue this routine using the supplied programs for the remaining 9 SET VOLUMES (25L to 350L ) doing 3 pipettings of each volume. Shuck and dispose of the QUADRA Pipetting Tips using the “SHUCK TIPS” program Tomtec Quadra 4 Operator Manual...

  • Page 200: Calibration Template" For The Verification Curve

    TIME TEMP º C (To nearest 0.2ºC) HUMIDITY % (To nearest 10%) BAROMETRIC PRESSURE (In. Hg) HEAD SERIAL NUMBER Fill in the tare weight of each weighing Verification Vessel in the area marked “TARE WT.”. Tomtec Quadra 4 Operator Manual...

  • Page 201: Calibration Instructions For 450 Μl Head

    RELATIVE HUMIDITY 50 % to 65 % 12.1 Importing an Existing Calibration Curve  a. Open the “QUADRA 4 COMMAND SYSTEM” program. b. In the upper menu bar select “UTILITIES”. c. On the pull down menu under “UTILITIES” select “IMPORT DATA”.

  • Page 202: Section Xi - Some Properties Of Liquids That Affect Pipetting Accuracy

    If the surface is high energy, it will want to be covered with a liquid because this interface will lower its energy, and so on. NOTE: All of the above properties are affected by temperature. Tomtec Quadra 4 Operator Manual...

  • Page 203: To Compensate For These Properties Of Liquids, The Aspirate And Dispense Steps Have Options To Improve Performance

    Slow tip withdraws can compensate for liquids with high wetting properties (liquids that tend to stick to the outside of the tip). Tip-touch option can remove drops of liquids with high surface tension that cling to the end of the tip. Tomtec Quadra 4 Operator Manual...

  • Page 204: Section Xii - Head Removal, Installation And Alignment

    Place the head in the storage stand. Never stand the head on the tips or place it where the tips may be damaged. Tomtec Quadra 4 Operator Manual...

  • Page 205: Top Front View (Open Panel)

    Close the front panel and turn on the power. The stage needs 24VDC to work and the front door interlock shuts it off. Initialize the Quadra 4. Put a nest with a 384 well plate on the station over the stage. The 384 well plate is used for the alignment of either 96 or 384 well heads.

  • Page 206: Head Installation

    Secure the B Q96 control board mounting plate, if necessary, and install the top cover. NOTE: The software will configure to the head installed. 3.0 Head Installation Q96 “B” board mounting plate Hose Fitting 3/8” allen wrench head Electrical Connectors (2) Tomtec Quadra 4 Operator Manual...
  • Page 207 Tomtec Quadra 4 Operator Manual...

  • Page 208: 450Μl Backfill Head Back View

    5.0 450µL Backfill Head Back View Tomtec Quadra 4 Operator Manual...

  • Page 209: Tip Block Assembly

    6.0 Tip Block Assembly Tomtec Quadra 4 Operator Manual...

  • Page 210: 55Μl Sv Head

    7.0 55µL SV Head Tomtec Quadra 4 Operator Manual...

  • Page 211: Sv Tip And Seal Assembly

    8.0 384 SV Tip and Seal Assembly TOMTEC Quadra 4 Operator’s Manual...

  • Page 212: Section Xiii - Routine & Preventive Maintenance By Operator

    Clean any spilled liquid promptly so it will not dry on moving parts. 1.0 Lubrication Kit Enclosed with the Quadra 4 is a tool designed to help insure optimum positive seal of the pipetting tips. It is used to lubricate the “O” rings on each tip pin with a small amount of mineral oil.

  • Page 213: Cleaning The Pipettor Tips

    2.0 Cleaning the Pipettor Tips (Applies to the 96SV and 384 heads) While using your Tomtec SV pipettor, wash the tips using the ultrasonic cleaner by performing the mix function while the tips are immersed in the flowing bath. The wash volume should exceed the transfer volume by at least 5 µL.

  • Page 214: Fuse Replacement

    Replace fuses according to the following chart: Return the fuse holder to its position in the power entry module by pushing in until it is seated. Re-connect the power cord to the power entry module. Turn Main Power switch on. TOMTEC Quadra 4 Operator’s Manual...

  • Page 215: Warranty

    Tomtec's liability is, in all cases, limited to the replacement price of its product. Tomtec shall not be liable for any other damages, whether consequential, indirect, and incidental, arising from the sale and use of its products.

  • Page 216: Quadra 4 Decontamination Form

    (Biological-infectious material or Radioactive isotope) are removed from the equipment in question. This form must be signed and accompany the Tomtec instrument when it is returned for service or repair. If not, the service department may be unable to perform service on the instrument.

  • Page 217: Tomtec Contact Information - If You Need Help

    Tomtec offers distribution and service in many countries throughout the world. Please contact Tomtec, USA (8:30 am to 4:30 pm EST), if you require technical support, or have questions/concerns regarding Tomtec products. Contact Tomtec in the United States:...

Table of Contents

Save PDF