Thermo Scientific Invitrogen Countess II FL Manuals

Manuals and User Guides for Thermo Scientific Invitrogen Countess II FL. We have 1 Thermo Scientific Invitrogen Countess II FL manual available for free PDF download: User Manual

Thermo Scientific Invitrogen Countess II FL User Manual (82 pages)

automated cell counter

Brand: Thermo Scientific | Category: Cash Counter | Size: 15 MB

Table of Contents
3
CHAPTER 1 About this Guide

7
- Audience
  7
- User Documentation
  7
- Text and Keyboard Conventions
  7
- User Attention Words
  8
- Safety Alert Words
  8
CHAPTER 2 Product Information

9
CHAPTER 3 Getting Started

12
- Installation
  12
  - Operating Environment
    12
  - Install the Instrument
    12
  - Turn on the Instrument
    13
- Load Profile
  14
  - Profiles Screen
    14
  - Automatic Instrument Functions
    14
  - Count Parameters
    15
  - Load a Profile
    16
  - Add/Edit a Profile
    16
  - Display of Profile Names
    18
- Prepare Sample
  19
CHAPTER 4 Cell Count and Cell Viability Assays

23
- Count Cells in Brightfield
  23
  - Capture and Count
    23
  - Next Steps
    25
- View Results
  25
  - Results Screen for Brightfield
    25
- Identify Objects Counted
  26
  - Advanced ™ Screen
    26
  - Identify Live and Dead Cells
    26
- Graph Count Results
  27
  - View Graph
    27
- Gate Count Results
  27
  - Adjust Screen
    27
- Save as New Protocol
  29
  - Edit and Save Profile as New Protocol
    29
CHAPTER 5 Fluorescence Assays

31
- Count Cell Fluorescence
  31
  - Overview
    31
  - Count Procedure
    31
  - Next Steps
    33
- View Results
  34
  - Results Screen for Cell Fluorescence Assays
    34
- Identify Objects Counted
  34
  - Advanced Screen
    34
  - Identify Cells Counted in Fluorescence Assays
    35
- Graph Count Results
  36
  - View Graph for Cell Fluorescence Assays
    36
- Gate Count Results
  37
  - Adjust Screen
    37
- Save as New Protocol
  39
  - Edit and Save Profile as New Protocol
    39
CHAPTER 6 Dilution Calculator

41
- Calculate Dilution
  41
  - Dilution Calculator
    41
CHAPTER 7 Save Results

44
- Save Count Results
  44
  - Save Screen
    44
  - Save Procedure
    44
- Report
  47
  - Report File
    47
CHAPTER 8 Instrument Settings

50
- Overview
  50
  - Instrument Settings Screen
    50
- Software Update
  50
  - Guidelines for Software Update
    50
  - Update the Countess ™ II/II FL Software
    51
- Date/Time
  52
  - Set the Date and Time
    52
- Change Light Cube
  54
  - Install or Change EVOS ™ Light Cube
    54
CHAPTER 9 Maintenance

56
- Instrument Care
  56
  - General Guidelines for Care
    56
  - Power Supply
    56
- Clean the Countess ™ II FL Automated Cell Counter
  56
  - Introduction
    56
  - Clean the Touch-Screen
    57
  - Clean the Instrument Case
    57
  - Decontaminate the Instrument
    57
- Set Nominal Focus
  57
  - Overview
    57
  - Set Nominal Focus
    58
APPENDIX A Troubleshooting

60
APPENDIX B Product Specifications

63
- Technical Specifications
  63
  - Physical Characteristics
    63
  - Optics
    63
  - Analysis Slide
    63
- EVOS ™ Light Cubes
  64
- LED Illumination
  64
APPENDIX C Ordering Information

66
- Accessory Products
  66
- Countess ™ II FL Automated Cell Counter and Accessories
  66
APPENDIX D CSV File Format

68
- CSV File Format, Explained
  68
  - Overview
    68
APPENDIX E Safety

71
- Safety Alert Words
  71
- Electrical Symbols
  72
- Safety Symbols
  72
- Environmental Symbols
  73
- Safety Labels on Instruments
  74
- General Instrument Safety
  74
- Chemical Safety
  76
- Chemical Waste Safety
  76
  - Chemical Waste Hazard
    76
  - Chemical Waste Safety Guidelines
    77
  - Waste Disposal
    77
- Electrical Safety
  77
- Biological Hazard Safety
  78
- Safety and Electromagnetic Compatibility (EMC) Standards
  79
- Documentation and Support
  80
- Customer and Technical Support
  80
- Limited Product Warranty
  80

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Thermo Scientific Invitrogen Countess II FL Manuals

Thermo Scientific Invitrogen Countess II FL User Manual (82 pages)

Table of Contents

CHAPTER 1 About this Guide

CHAPTER 2 Product Information

CHAPTER 3 Getting Started

CHAPTER 4 Cell Count and Cell Viability Assays

CHAPTER 5 Fluorescence Assays

CHAPTER 6 Dilution Calculator

CHAPTER 7 Save Results

CHAPTER 8 Instrument Settings

CHAPTER 9 Maintenance

APPENDIX A Troubleshooting

APPENDIX B Product Specifications

APPENDIX C Ordering Information

APPENDIX D CSV File Format

APPENDIX E Safety

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