SCIEX PA 800 Plus Manuals

Manuals and User Guides for SCIEX PA 800 Plus. We have 3 SCIEX PA 800 Plus manuals available for free PDF download: Development Manual, System Maintenance Manual, System Overview Manual

SCIEX PA 800 Plus Development Manual (158 pages)

Pharmaceutical Analysis System Methods Development Guide

Brand: SCIEX | Category: Laboratory Equipment | Size: 4 MB

Example
2
Table of Contents
3
CHAPTER 1 About this Guide

9
- Overview
  9
- Data
  10
  - Purpose of These Files
    11
  - Using the Files
    11
- Instructions for Using the Sample
  10
CHAPTER 2 Introduction to Capillary

13
- Overview
  13
  - General Description
    13
  - Electrophoresis
    13
- Common Modes of Electrophoresis
  14
  - Capillary Zone Electrophoresis
    14
  - Micellar Electrokinetic Capillary Chromatography
    14
  - Capillary Gel Electrophoresis
    14
  - Capillary Isoelectric Focusing
    14
  - (Cze)
    14
  - (Cge)
    14
  - (Cief)
    14
  - Capillary Electrochromatography
    15
  - (Cec)
    15
CHAPTER 3 System Overview

17
- The Sample Handling System
  19
- Capillary Cartridge
  22
- Fluid Delivery, Power Supply and Syringe Pump
  24
  - High Voltage Power Supply
    24
  - LED Indicators
    24
  - Cartridge and Sample Cover
    24
  - Interlock
    24
  - Interlocks
    24
- UV Detector Optics
  25
- The Photodiode Array (PDA) Detector
  26
- Detector
  27
  - Laser Module
    29
    
    488 Nm Laser Module
    29
- Laser-Induced Fluorescence (LIF)
  27
CHAPTER 4 32 Karat Software

31
- Starting the Software
  31
  - Tool Bar
    32
  - Menu Bar
    32
- Creating and Configuring an Instrument
  32
  - Creating a New Instrument
    33
  - Configuring the New Instrument
    33
  - Auto Configuration
    35
  - Configuration Options
    37
    
    Analysis Options
    37
    
    Pda
    37
    
    System Suitability
    37
    
    Qualitative Analysis
    38
    
    Caesar Integration
    38
  - Instrument Options
    38
    
    Standard CE
    38
    
    Cec/Lc
    38
  - Manual Configuration
    39
    
    Manual Configuration of the UV Detector
    39
    
    Manual Configuration of the PDA and LIF Detectors
    40
- Instrument
  17
CHAPTER 5: Direct Control

41
- Introduction
  41
- Direct Control Window
  41
- Exercise: Conditioning the Capillary
  44
CHAPTER 6: Creating and Editing a Method

49
- Introduction
  49
- Creating and Editing a Method
  49
- Initial Conditions Tab
  50
- Detector Initial Conditions
  52
  - UV Detector Initial Conditions
    52
  - Electropherogram Channel
    53
  - Filter
    53
  - Relay 1 and Relay 2
    53
  - Absorbance Signal
    53
- PDA Detector Initial Conditions
  54
  - Electropherogram Scan Data
    54
  - Filter
    54
  - Relay 1 and Relay 2
    54
  - Reference Channel
    55
  - Absorbance Signal
    55
  - Electropherogram Channel Data
    55
- LIF Detector Initial Conditions
  56
  - Electropherogram Channel 1 and 2
    56
  - Signal
    57
  - Data Rate
    57
  - Relay 1 and 2
    57
- Time Program
  57
  - Separate Dialog
    58
  - Rinse Dialog
    61
  - Inject Dialog
    61
  - Autozero
    63
  - Stop Data
    63
  - End
    63
  - Saving the Method
    67
CHAPTER 7 Running the System

69
- Single Run and Sequence Runs
  69
  - Materials Needed
    69
- Single Run
  70
- Programming a Sequence
  72
- Running the Sequence
  77
- Sequence Validation
  78
CHAPTER 8 Integration

81
- Description
  81
- Integrating Data
  82
- Optimizing Integration
  84
  - Integration Parameters
    85
  - Width
    86
  - Threshold
    87
    
    Integrating a Peak Cluster
    88
    
    Integration off
    89
- Integration Results and Reports
  91
CHAPTER 9 Calibration

93
- Introduction
  93
- Analysis Components
  93
  - Qualitative Analysis
    93
  - Quantitative Analysis
    93
- Creating a Calibration
  94
  - Developing a Calibration Method
    94
  - Generating the Calibration Curve
    97
- Analyzing Unknowns
  102
CHAPTER 10: Qualitative Analysis

103
- Data Analysis and Reporting
  103
CHAPTER 11: Mobility

107
- Functions of Mobility
  107
- Mobility Markers
  108
  - Assigning Mobility Values
    108
  - Mobility Plot
    110
- Demonstration: Using the Mobility Features
  111
- Peak Identification by Mobility
  113
CHAPTER 12: Creating Reports

115
- Introduction
  115
- Creating a Method Custom Report
  116
- Modifying a Report Template
  116
  - Saving a Report as a Template
    119
    
    Saving a Report as a Default Report
    120
  - Report Templates
    120
  - Method Custom Report Templates
    120
  - Sequence Custom Report Templates
    120
    
    Standard Report Templates
    121
    
    Sequence Reports
    121
APPENDIX A: Additional Resources

123
- 32 Karat Software Online Help
  123
- Installation and Maintenance Guide
  123
- SCIEX Service
  123
- Other References
  123
APPENDIX B: PA 800 Plus System Calculations

125
- Calculations
  125
  - CE Calculations
    125
  - Ohm's Law
    125
  - Corrected Migration Time
    125
  - Corrected Area
    126
  - Mobility
    126
APPENDIX C: Understanding PDA Data

129
- Overview
  129
- Mixed View
  131
- Contour Plot
  132
- Spectrum
  132
- Electropherogram
  132
- 3D Plot
  132
APPENDIX D: System Administration

135
- Overview
  135
APPENDIX E: Vial Incrementing

137
- Introduction
  137
- Examples of Use
  139
  - Example 2
    142
- Summary
  145
APPENDIX F: Sequence Vial Report

147
- Overview
  147
  - Sequence Vial Increment Preview
    147
- Sequence Vial Report-Confirmed
  149
- Revision History
  157

SCIEX PA 800 Plus System Maintenance Manual (86 pages)

Pharmaceutical Analysis System

Brand: SCIEX | Category: Medical Equipment | Size: 2 MB

Table of Contents
3
Overview
5
Introduction
6
Install UV or PDA Detectors
7
Calibrate the PDA Detector
10
Install UV Detector Wavelength Filters
11
Integrated 488 Nm Solid-State Laser
17
Capillary Cartridge
25
- Rebuild a Capillary Cartridge
  25
- Install a Capillary Cartridge into the PA 800 Plus System
  44
Universal Vials and Caps
46
- Fill the Universal Vials
  46
- Fill the Micro Vials
  47
Maintenance Procedures
49
- Opening Levers, Electrodes, and the Interface Block
  50
- Clean Electrodes, Opening Levers, and the Interface Block
  53
Coolant
54
Fiber Optics (UV and PDA Detectors)
56
Lamp
58
Instrument Care
65
- LIF Care and Inspect the LIF
  65
- How to Clean the How to Store the
  65
- Maintenance
  65
- Lif
  65
- Replace the Quad-Rings
  66
- Replace the Fuses
  67
Connect the System to a Mass
69
Spectrometer
69
- Install the Capillary in the Standard
  70
- Cartridge
  70
- Ground Connection
  72
CE-MS/MS Mode Cartridge Procedures
69
APPENDIX A: LIF Test MIX Calibration and Performance Tests

73
- (Ccf)
  73
- Calibration Correction Factor
  73
- LIF Test MIX Calibration and Performance
  73
- Relative Fluorescent Units (RFU)
  73
- Tests
  73
- Applying the CCF
  74
- Automatic Calibration
  74
- Calibration Method
  75
- Capillaries
  76
- LIF Calibration for Different Dimension
  76
- Non-Fluorescent Solutions
  76
- LIF Calibration for Different Fluorescent
  77
- Solutions
  77
- Standard System Performance
  77
- Test
  77
- Troubleshooting
  79
APPENDIX B: Evaluating PA 800 Plus System UV Filters

81
- Filters
  81
- Overview
  81
- Revision History
  85

SCIEX PA 800 Plus System Overview Manual (56 pages)

Pharmaceutical Analysis System

Brand: SCIEX | Category: Analytical Instruments | Size: 2 MB

Table of Contents
3
CHAPTER 1 Safety, Notices and Labels

7
- Alerts for Warning, Caution, Important, and
  8
- Instrument Safety Precautions
  8
  - Moving Parts or Sharp
    8
  - Note
    8
  - Objects
    8
- Chemical Precautions
  9
- Safety Symbols and Labels
  9
  - High Voltage Electric Shock Risk
    9
  - Sharp Object Label
    9
  - Symbol
    9
  - General Biohazard Symbol
    10
  - Caution, Biohazard Label
    10
  - Class 1 Laser Caution Label
    10
- Rohs Notices
  10
  - China Rohs Caution Label
    10
  - China Rohs Environmental
    11
- Other Instrument Labels
  11
  - Recycling Label (WEEE)
    11
  - Label
    11
  - Disposal of Devices Containing Mercury
    12
  - Components
    12
Overview
7
General Safety Information
7
CHAPTER 2 System Overview

13
- Sample Handling System
  15
- Capillary Cartridge
  17
- Fluid Delivery, Power Supply, and Syringe Pump
  18
  - High Voltage Power Supply
    18
  - LED Indicators
    18
  - Cartridge and Sample Cover
    18
  - Interlocks
    18
- UV Detector Optics
  19
- The Photodiode Array (PDA) Detector
  20
- The Laser Induced Fluorescence (LIF) Detector
  21
  - Laser Module
    22
    
    488 Nm Laser Module
    22
- Supported Applications
  23
- Instrument
  13
CHAPTER 3: System Procedures and Training Guide

25
- Controller and Instrument Start up
  25
  - Controller/Network Logon
    25
  - License Key
    25
- PA 800 Plus Software
  26
- Enterprise Window
  26
  - Instrument Start up
    27
- System Administration
  27
  - Configuration
    29
  - Online Versus Offline
    30
- How to Load the Cartridge and Samples
  30
- How to Run a Method
  33
- Analysis
  36
  - Area Calculation
    40
  - Annotations
    41
- Peak Identity
  42
- Run Multiple Methods with a Sequence
  42
- Viewing Reports
  47
  - Standard Reports
    47
  - Method Custom Reports
    47
  - Sequence Custom Report
    47
  - Advanced Reports
    47
  - Suitability Reports
    49
  - Viewing and Printing Reports
    49
- Maintenance
  49
APPENDIX A: Specifications

51
- PA 800 System
  51
- Computer Requirements
  52
- UV Detector
  52
- PDA Detector
  53
- Revision
  55

SCIEX Categories

Laboratory Equipment Portable Generator Medical Equipment Control Unit Analytical Instruments

More SCIEX Manuals

SCIEX PA 800 Plus Manuals

SCIEX PA 800 Plus Development Manual (158 pages)

Table of Contents

CHAPTER 1 About this Guide

CHAPTER 2 Introduction to Capillary

CHAPTER 3 System Overview

CHAPTER 4 32 Karat Software

CHAPTER 5: Direct Control

CHAPTER 6: Creating and Editing a Method

CHAPTER 7 Running the System

CHAPTER 8 Integration

CHAPTER 9 Calibration

CHAPTER 10: Qualitative Analysis

CHAPTER 11: Mobility

CHAPTER 12: Creating Reports

APPENDIX A: Additional Resources

APPENDIX B: PA 800 Plus System Calculations

APPENDIX C: Understanding PDA Data

APPENDIX D: System Administration

APPENDIX E: Vial Incrementing

APPENDIX F: Sequence Vial Report

SCIEX PA 800 Plus System Maintenance Manual (86 pages)

Table of Contents

APPENDIX A: LIF Test MIX Calibration and Performance Tests

APPENDIX B: Evaluating PA 800 Plus System UV Filters

SCIEX PA 800 Plus System Overview Manual (56 pages)

Table of Contents

CHAPTER 1 Safety, Notices and Labels

CHAPTER 2 System Overview

CHAPTER 3: System Procedures and Training Guide

APPENDIX A: Specifications

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SCIEX Categories