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Xdrop
Samplix Xdrop Manuals
Manuals and User Guides for Samplix Xdrop. We have
2
Samplix Xdrop manuals available for free PDF download: Manual
Samplix Xdrop Manual (23 pages)
Droplet PCR (dPCR)
Brand:
Samplix
| Category:
Laboratory Equipment
| Size: 1.76 MB
Table of Contents
Table of Contents
2
Chapter 1: Xdrop
3
Targeted Enrichment Overview
3
Xdrop™ Instrument Overview
4
Required Items for Xdrop™ Dpcr
7
Suggested Samplix Products
7
Chapter 2 : Dpcr - General Considerations
9
Dpcr Assay Design
9
Target Sequence
9
Primer Design Guidelines
10
DNA Sample Preparation
10
Primer Optimization
11
Chapter 3: Dpcr Setup
12
Preparations for Dpcr
12
Setup of Dpcr Reaction
12
Prepare Dpcr Cartridge
14
Collect Generated Droplets
19
Chapter 4: Single DNA Molecule Detection and Sorting of Droplets
20
Requirements for Flow Cytometer Analyzer and Cell Sorter
20
Notes to Operator
20
Preparation of Droplets for Flow Cytometry
20
Flow Cytometry Analysis of Dpcr Droplets
21
Sorting of PCR Positive Dpcr Droplets
23
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Samplix Xdrop Manual (22 pages)
Brand:
Samplix
| Category:
Laboratory Equipment
| Size: 1.98 MB
Table of Contents
Table of Contents
2
Chapter 1: Xdrop™ at a Glance
3
Targeted Enrichment Overview
3
Xdrop™ Instrument Overview
4
Required Items for Xdrop™ Dmda
7
Suggested Samplix Products
7
Chapter 2: General DNA Amplification by Dmda
9
Break Sorted Droplets
9
Set up Dmda Reaction
10
Load the Dmda Cartridge
11
Generate Dmda Droplets on the Xdrop™ Instrument
14
Chapter 3: Evaluation of Targeted Enrichment
19
Quantify Total DNA
19
Evaluate the Enrichment of Target DNA
20
Calculate Fold Enrichment of Target DNA
21
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