PerkinElmer Nuance 3.0.2 Manuals

Manuals and User Guides for PerkinElmer Nuance 3.0.2. We have 1 PerkinElmer Nuance 3.0.2 manual available for free PDF download: User Manual

PerkinElmer Nuance 3.0.2 User Manual (132 pages)

MULTISPECTRAL IMAGING SYSTEM

Brand: PerkinElmer | Category: All in One Printer | Size: 8.46 MB

Table of Contents
3
Part I Welcome to Nuance
6
Part II Introduction to Multispectral Imaging
7
Light
7
Human Perception of Light Intensity and of Color
8
Light Absorbance and Reflection
9
Fluorescence
10
The Multispectral Solution
12
Nuance FLEX Multispectral Imaging
15
Part III Getting Started with Nuance
17
Operator and Equipment Safety
17
About this Manual
18
CE, CSA, and UL Testing and Certification
19
Nuance Imaging Module
19
Installing & Starting Nuance
22
Understanding the Nuance Work Area
23
Window Layout
25
Specifying Nuance Hardware Settings
27
Calibrating Pixel Size
29
Using Low Screen Resolution Mode
30
Reinitializing Nuance Hardware
30
Part IV Method Development
31
Methods for Building Spectral Libraries
31
Saving Spectral Libraries
35
Saving Protocols
35
Importing Spectra into a Library
36
Practice Exercise
37
Part V Brightfield and Fluorescence Imaging
40
Setting up Your Microscope for Brightfield Imaging
40
Setting up Your Microscope for Fluorescence Imaging
41
About Optical Density Images
42
Tips for Obtaining Quantitative Results
43
Acquiring Images Using a Saved Nuance Protocol
43
Viewing a Live Image Stream
44
Selecting Camera Binning and a Region of Interest
44
Specifying the Current Wavelength and Exposure
45
Acquiring Cubes in Brightfield
47
Acquiring RGB (Color) Images
48
Acquiring Fluorescence Cubes
49
Acquiring a Mono Image (Snapshot)
52
Assigning Sample Ids and Notes
52
Acquiring Timed Sequences of Image Cubes
53
Saving Images and Image Cubes
54
Viewing Cube Information
55
Extracting an Image Plane from a Cube
56
Part VI Unmixing Spectral Images
57
Tips for Accurate Unmixing
57
Opening a Spectral Library
57
Opening an Image Cube
58
Computing and Unmixing Spectra Automatically
58
Computing and Unmixing Spectra Manually
61
Saving an Unmixed Result Set
65
Working with Saved Result Sets
65
Checking Your Spectral Library
66
Subtracting Spectra from a Cube
67
Using Line Profiles to Analyze Signals
67
Comparing Multiple Images
69
Processing a Batch of Cubes
72
Part VII Quantifying Results
75
Measuring Regions
75
Ignoring Smaller Regions
80
Adjusting Region Transparency and Color
80
Understanding Region Measurements
80
Part VIII Customizing Spectral Displays
83
Adjusting Brightness and Contrast Levels
83
Applying Overlays
84
Adjusting a Cube's RGB Mapping
84
Changing Components in a Composite Image
85
Advanced Display Controls
86
Displaying the Intensity Legend
88
Part IX Macros
89
Overview of the Macros Dialog Box
89
Running Macros
91
Recording Macros
91
Saving Macros
91
Part X Co-Localization Staining Detection
92
Opening a Dataset for Co-Localization Analysis
92
Adjusting Threshold Mask Values
93
Selecting Markers for Co-Localization
94
Saving and Loading Co-Localization Settings
95
Interpreting the Statistics
96
Customizing the Statistics Display
97
Drawing Regions of Interest
97
Customizing the Composite Image Display
98
Copying Images and Data
99
Exporting Images and Data
99
Part XI Faqs
100
Part XII Troubleshooting
102
Part XIII System Specifications & Dimensions
104
Part XIV Filter Selection Guide
107
Part XV Metamorph Support
108
Installing and Configuring Metamorph
109
Installing Nuance with Metamorph Support
112
Import the Journal Suite for Metamorph
113
Troubleshooting
116
Part XVI Koehler Aligning the Microscope
117
Part XVII about Legacy Hardware
118
Part XVIII Windows User Management
119
Part XIX Software EULA
123
Index
126

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PerkinElmer Nuance 3.0.2 Manuals

PerkinElmer Nuance 3.0.2 User Manual (132 pages)

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