Miltenyi Biotec autoMACS Pro Separator Manuals

Miltenyi Biotec autoMACS Pro Separator Manuals

Manuals and User Guides for Miltenyi Biotec autoMACS Pro Separator. We have 1 Miltenyi Biotec autoMACS Pro Separator manual available for free PDF download: User Manual

Miltenyi Biotec autoMACS Pro User Manual

Miltenyi Biotec autoMACS Pro User Manual (166 pages)

Brand: Miltenyi Biotec | Category: Water Filtration Systems | Size: 4 MB

Table of Contents

Table of Contents
6
Important Safety Information

11
- General Safety Instructions
  14
Wichtige Sicherheitsinformationen

19
Información Importante de Seguridad

29
Informations de Sécurité Importantes

39
Importanti Istruzioni DI Sicurezza

49
1 Introduction

59
- MACS® Technology - the Gold Standard in Cell Separation
  59
- The Automacs Pro Separator
  60
2 Installation

63
- Components Included in Delivery
  64
- Materials Required for Operation
  64
- Automacs Pro Separator Accessories
  66
- Unpacking the Automacs Pro Separator
  66
- Installing and Replacing Fluid Bottles
  69
- Installing the MACS Minisampler, MACS Reagent Rack 4 and Tube Rack
  71
- Switching on or off
  
  75
  - Switching on the Instrument
    75
  - When Switching on the Instrument for the First Time
    75
  - Installing the Pump Syringe
    77
  - Installing Automacs Columns
    78
  - Set the Date and Time
    80
  - Testing the Bar Code Scanner
    80
  - Checking Fluid Levels
    81
  - Test the Needle Calibration
    81
  - Priming
    82
  - Long-Term Storage
    83
- The Automacs® Pro User Interface
  
  85
  - Shutting down the Instrument
    83
  - Sleep Modus
    83
  - The Status Menu
    86
  - Fluid Bottle Status
    87
  - Sample Status
    87
  - Column Status
    88
  - Tube Rack Status
    88
  - MACS Minisampler Status
    89
  - The Reagent Menu
    89
  - Entry of Reagents with the 2D Code Reader
    90
  - Manual Entry of Reagents
    91
  - Deleting a Single Reagent from the Reagent List
    92
  - Deleting Reagents
    92
  - Deleting the Entire Reagent List
    92
  - The Separation Menu
    92
  - Apply an Assigned Labeling Option to Other Samples
    93
  - Assign a Labeling Option to a Single Sample
    93
  - Assign One Labeling Option to Several Samples
    93
  - Assigning Sample Volumes
    93
  - Sample Labeling Options
    93
  - Sample Rack Template
    93
  - Assigning a Cell Separation Program
    94
  - Assigning a Wash Program
    94
  - Delete Program Combinations
    94
  - The Log List Menu
    95
  - Special
    96
  - The Option Menu
    96
  - User Settings
    97
5 Calibration

99
- Calibrate the Needle Position
  99
- Running a Test Calibration
  99
- Calibrating the Needle Position
  103
- Calibrate the Washing Station: Uptake Needle (Step 1/5)
  103
- Calibrate the Washing Station: NEG Needle (Step 2/5)
  104
- Calibration of the Z-Axis (Step 3/5)
  105
- Calibration of the X-Axis (Step 4/5)
  106
- Test Current Calibration Settings (Step 5/5)
  106
- Calibrating the Uptake Volume
  108
- Cell Separation
  
  109
  - Cell Labeling Strategies
    110
  - Cell Separation Strategies
    110
  - Direct Magnetic Labeling
    110
  - Indirect Magnetic Labeling
    110
  - Positive Selection
    110
  - Depletion
    111
  - Depletion Followed by Positive Selection
    111
  - Sequential Sorting
    111
  - Untouched Isolation
    111
  - Positive Selection Programs
    112
  - Separation Programs
    112
  - Two Subsequent Positive Selections
    112
  - Depletion Programs
    113
  - Maintenance Programs
    116
  - Wash and Maintenance Programs
    116
  - Wash Programs
    116
  - Preparation of Single-Cell Suspensions
    117
  - Adjusting Sample Volumes
    118
  - Selecting the Appropriate Tube Rack
    120
  - Interrupting Cell Separation
    121
  - Monitoring the Separation Process
    121
  - Program Status
    121
  - Fluid Bottle Illumination
    122
  - Priming the Automacs Pro Separator
    122
  - Cell Labeling and Separation
    123
  - Cell Separation after Manual Labeling
    124
  - Cell Separation Using the Autolabeling Function
    124
  - Deleting a Template
    125
  - Loading a Template
    125
  - Saving a Reagent Template
    125
  - Saving a Separation Template
    125
  - Working with Templates
    125
  - Shutting down the Instrument
    126
7 Maintenance

127
- Daily Maintenance
  127
- Instrument Priming
  127
- Automatic Instrument Priming at Startup
  128
- Clean Uptake/Outlet Ports
  128
- Periodic Maintenance
  129
- Column Exchange
  129
- Exchange of Hydrophobic Air Filters
  130
- Clean the Pump Syringe
  130
- Cleaning the Washing Station
  133
- Instrument Disinfection
  134
- Corrective Maintenance
  135
- Valve Exchange
  135
- Dilutor Valve Exchange
  138
- Exchange of the Peristaltic Pump Head
  142
- Exchange of Tubing System
  143
- Exchange of Fuses
  143
- Quality Control
  
  145
  - Cell Recovery
    145
  - Purity of Isolated Cell Population
    146
  - Viability of the Cells
    146
9 Troubleshooting

147
- Issues with Moving Hardware
  147
- Hardware Issues Not Indicated by an Error Screen
  148
- Column Leakage
  148
- Tubing Leakage
  148
- Pump Syringe Leakage
  149
- Pump Syringe Is Filled with Air During Operation
  149
- Washing Station Overflow
  149
- Outlet Port Is Clogged
  150
- Contamination of Tubing System
  150
- MACS Minisampler Does Not Move Properly
  150
- Touchscreen Remains Dark
  150
- Disruption of Power Supply During Cell Separation
  151
- Rescue Procedure a
  151
- Rescue Procedure B
  151
- Hardware Issues Indicated by an Error Screen
  152
10 Technical Data and Specifications

157
- Compliance with FCC Rules
  159
- EC Declaration of Conformity
  160
11 Technical Support

161
12 Limited Warranty

163

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