Lonza AAF-1001B Transfection Unit Manuals

Manuals and User Guides for Lonza AAF-1001B Transfection Unit. We have 2 Lonza AAF-1001B Transfection Unit manuals available for free PDF download: Manual, Hardware Manual

Lonza AAF-1001B Hardware Manual

Lonza AAF-1001B Hardware Manual (40 pages)

Brand: Lonza | Category: Laboratory Equipment | Size: 1.67 MB

Table of Contents

Table of Contents
3
Nucleofector Technology
4
How It Works
4
Transfection of any Cell
4
Transfection of any Substrate
4
Moving Transfection into the Fourth Dimension
5
Experience the Advanced Nucleofector® Platform
5
Operating Instructions
6
Restrictions
6
Maintenance
6
Safety Instructions - Please Read Carefully
6
Consignes de Sécurité - à Lire Attentivement
7
Waste Disposal
8
Nucleofector® System Components
9
Set-Up Instructions
10
General Use Instructions
10
Turning on the 4D-Nucleofector® System
10
Operating Software
10
Turning off the 4D-Nucleofector® System
11
General Instructions for Running a
11
Nucleofection® Experiment
11
Overview
11
Unit and Vessel Selection
11
Experiment Parameters - Overview
12
Using a Predefined Experiment
13
Nucleofector® X Unit
14
Defining a New Experiment
14
Loading Samples
16
Running the Experiment
16
Nucleofector® y Unit
17
Defining a New Experiment
17
Loading Samples
18
Running the Experiment
18
Nucleofector® 96-Well Unit
19
Defining a New Experiment
19
Loading Samples
21
Running the Experiment
21
Nucleofector® LV Unit
22
Using the 1 Ml Nucleocuvette® Cartridge (Fixed Volume)
23
Defining a New Experiment
23
Loading Samples
24
Running the Experiment
24
Using the LV Nucleocuvette® Cartridge (Scalable Volume)
25
Defining a New Experiment
26
Mounting an LV Nucleocuvette® Cartridge
26
Loading Samples
28
Running the Experiment
30
Additional Settings and Options
31
Settings Menu on the Right
31
System Settings
31
Cleaning the Nucleofection® Vessel Tray (X, Y, or 96-Well Unit)
31
Left Menu
32
E Xperiments
32
Results
32
Custom Cell Type Codes
32
Lonza Programs
33
Firmware Update
33
Troubleshooting
34
Suboptimal Transfection Results
34
Rescue of Samples (X and y Unit)
35
Error Codes of the 4D-Nucleofector ® System
36
Color Coding of Wells
36
Error Codes
36
Arc Discharge Correction
37
Purchaser Notification
37
Representations and Warranties
37
Technical Specifications
39

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Lonza AAF-1001B Manual

Lonza AAF-1001B Manual (40 pages)

Brand: Lonza | Category: Laboratory Equipment | Size: 2.16 MB

Table of Contents

Table of Contents
3
The Nucleofector™ Technology
4
Operating Instructions
6
Restrictions
6
Maintenance
6
Waste Disposal
7
Nucleofector™ System Components 8 5 Purchaser Notification
8
Set-Up Instructions
9
General Use Instructions 10 6 Technical Specifications
10
Turning on the 4D-Nucleofector™ System
10
Adjusting the Position of the Touch Screen
10
Operating Software
11
Turning off the 4D-Nucleofector™ System
11
General Instructions for Running a Nucleofection Experiment
12
Overview
12
Unit and Vessel Selection
13
Experiment Parameters - Overview
13
Using a Predefined Experiment
14
Nucleofector™ X Unit
15
Defining a New Experiment
15
Loading Samples
16
Running the Experiment
17
Nucleofector™ y Unit
18
Defining a New Experiment
18
Loading Samples
19
Running the Experiment
19
Nucleofector™ LV Unit
20
Using the 1 Ml Nucleocuvette™ Cartridge
21
(Fixed Volume)
21
Defining a New Experiment
21
Loading Samples
21
Running the Experiment
22
Using the LV Nucleocuvette™ Cartridge (Scalable Volume)
23
Mounting an LV Nucleocuvette™ Cartridge
23
Defining a New Experiment
25
Running the Experiment
27
Additional Settings and Options
28
Experiments
28
Results
29
Custom Programs
29
Synchronize
30
Cleaning the Nucleofection Vessel Tray
30
(X or y Unit)
30
Lonza Programs
30
Firmware Update
30
PC Editor Software
31
Suboptimal Transfection Results
33
96-Well Shuttle™ Mode
33
Troubleshooting
33
Rescue of Samples (X and y Unit)
34
Nucleofector™ System
35
Safety Instructions - Please Read Carefully 7 4.1 Arc Discharge Correction
35

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