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nanowizard afm
JPK instruments nanowizard afm Manuals
Manuals and User Guides for JPK instruments nanowizard afm. We have
1
JPK instruments nanowizard afm manual available for free PDF download: Handbook
JPK instruments nanowizard afm Handbook (59 pages)
atomic force microscope
Brand:
JPK instruments
| Category:
Laboratory Equipment
| Size: 2.96 MB
Table of Contents
Table of Contents
3
1 Introduction
5
About this Handbook
5
What Is an Atomic Force Microscope
5
Scanning Probe Microscopy
6
Atomic Force Microscopy
7
AFM Cantilevers
8
2 Imaging Modes
9
Feedback and Imaging Control
9
Amplitude Feedback in Dynamic Modes
9
Another Way of Thinking about Imaging Modes
10
Operation
12
Phase Imaging
13
Force Adjustment in Imaging Modes
14
Applications
15
3 Force Spectroscopy
16
Introduction
16
Data Processing for Analysis
17
Applications
20
4 More about Cantilevers
22
General Points
22
Handling Information
22
Cantilever Types for Different Imaging Modes
23
Tip Modification
24
Spring Constant
25
5 Cell Imaging
28
AFM in Relation to Other Cell Imaging Techniques
28
Sample Preparation
29
Imaging Modes
31
Critical Imaging Parameters
32
Using the Oscilloscope to Optimize the Imaging Parameters
32
Artifacts
34
6 Single Molecule Imaging
35
Preparation Is Key
35
Imaging Hints - Intermittent Contact Mode in Liquid
37
Imaging Hints - Contact Mode in Liquid
38
Imaging Hints - Imaging in Air
39
Simple DNA Protocol for Imaging Tests
39
7 Artifacts
41
Tip Shape Issues
41
Artifacts from Damaged Tips
42
Contamination
43
Other Imaging Considerations
43
8 Useful Physics for SPM
45
The Cantilever Resonance
45
Thermal Noise Spring Constant Calibration
45
Young's Modulus of Materials
49
9 Useful Chemistry and Sample/Tip Preparations
51
Cleaning Cantilevers and Tips
51
Silanization and APTES Treatment
52
Home Made Gel Packs for Cantilever Storage
53
Suppliers of AFM Accessories
53
10 References
55
General AFM Papers
55
Spring Constant Calibration References
57
Books
58
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